Eleutheroside E(EE)is a monomer compound isolated from Acanthopanax senticosus,which has functions of antifatigue,antioxidation,and immune regulation.However,the function of EE in inhibiting cancer has rarely been rep...Eleutheroside E(EE)is a monomer compound isolated from Acanthopanax senticosus,which has functions of antifatigue,antioxidation,and immune regulation.However,the function of EE in inhibiting cancer has rarely been reported.In this study,we used bioinformatics to determine the role and mechanisms of EE in inhibiting breast cancer(BRCA),prostate cancer(PRAD),lung cancer(LUAD),and bladder cancer(BLCA).Finally,the MTT method was used to evaluate the inhibitory effect of EE on breast cancer MDA-MB-231 cells,and the target genes were determined by qRT-PCR.The results showed that the survival rate of MDA-MB-231 cells was reduced by 27.33%after 400μg/mL EE treatment.The results of qRT-PCR showed that EE down-regulated target genes in breast cancer cells,which was consistent with our predicted results.Moreover,our results indicated that EE may have impact on the transcription and translation of BLCA cells by targeting ADCY2,ADCY5 and JUN.In BRCA,we identified three targets for EE.In addition,EE affected DNA repair and the development of BRCA by affecting the expression of RAD51.In LUAD,we identified 9 targets for EE,8 of which were related to DNA repair.EE inhibited PRAD by targeting ADCY8.Our research was the first report on the targets and mechanisms of EE for suppressing cancer combined with clinical data.Our research also provides theoretical support for using EE to suppress cancer.展开更多
Eleutheroside B or E, the main component of Acanthopanax, can relieve fatigue, enhance memory, and improve human cognition. Numerous studies have confirmed that high doses of acetylcholine significantly attenuate clin...Eleutheroside B or E, the main component of Acanthopanax, can relieve fatigue, enhance memory, and improve human cognition. Numerous studies have confirmed that high doses of acetylcholine significantly attenuate clinical symptoms and delay the progression of Alzheimer＇s disease. The present study replicated a rat model of aging induced by injecting quinolinic acid into the hippocampal CA1 region. These rats were intraperitoneally injected with low, medium and high doses of eleutheroside B or E （50, 100, 200 mg/kg）, and rats injected with Huperzine A or PBS were used as controls. At 4 weeks after administration, behavioral tests showed that the escape latencies and errors in searching for the platform in a Morris water maze were dose-dependently reduced in rats treated with medium and high-dose eleutheroside B or E. Hematoxylin-eosin staining showed that the number of surviving hippocampal neurons was greater and pathological injury was milder in three eleutheroside B or E groups compared with model group. Hippocampal homogenates showed enhanced cholinesterase activity, and dose-dependent increases in acetylcholine content and decreases in choline content following eleutheroside B or E treatment, similar to those seen in the Huperzine A group. These findings indicate that eleutheroside B or E improves learning and memory in aged rats. These effects of eleutheroside B or E may be mediated by activation of cholinesterase or enhanced reuse of choline to accelerate the synthesis of acetylcholine in hippocampal neurons.展开更多
基金This research was supported by The National Key Research and Development Program of China(2017YFC1601900).
文摘Eleutheroside E(EE)is a monomer compound isolated from Acanthopanax senticosus,which has functions of antifatigue,antioxidation,and immune regulation.However,the function of EE in inhibiting cancer has rarely been reported.In this study,we used bioinformatics to determine the role and mechanisms of EE in inhibiting breast cancer(BRCA),prostate cancer(PRAD),lung cancer(LUAD),and bladder cancer(BLCA).Finally,the MTT method was used to evaluate the inhibitory effect of EE on breast cancer MDA-MB-231 cells,and the target genes were determined by qRT-PCR.The results showed that the survival rate of MDA-MB-231 cells was reduced by 27.33%after 400μg/mL EE treatment.The results of qRT-PCR showed that EE down-regulated target genes in breast cancer cells,which was consistent with our predicted results.Moreover,our results indicated that EE may have impact on the transcription and translation of BLCA cells by targeting ADCY2,ADCY5 and JUN.In BRCA,we identified three targets for EE.In addition,EE affected DNA repair and the development of BRCA by affecting the expression of RAD51.In LUAD,we identified 9 targets for EE,8 of which were related to DNA repair.EE inhibited PRAD by targeting ADCY8.Our research was the first report on the targets and mechanisms of EE for suppressing cancer combined with clinical data.Our research also provides theoretical support for using EE to suppress cancer.
基金supported by the Foundation from Department of Education of Hubei Province,No.D20111903
文摘Eleutheroside B or E, the main component of Acanthopanax, can relieve fatigue, enhance memory, and improve human cognition. Numerous studies have confirmed that high doses of acetylcholine significantly attenuate clinical symptoms and delay the progression of Alzheimer＇s disease. The present study replicated a rat model of aging induced by injecting quinolinic acid into the hippocampal CA1 region. These rats were intraperitoneally injected with low, medium and high doses of eleutheroside B or E （50, 100, 200 mg/kg）, and rats injected with Huperzine A or PBS were used as controls. At 4 weeks after administration, behavioral tests showed that the escape latencies and errors in searching for the platform in a Morris water maze were dose-dependently reduced in rats treated with medium and high-dose eleutheroside B or E. Hematoxylin-eosin staining showed that the number of surviving hippocampal neurons was greater and pathological injury was milder in three eleutheroside B or E groups compared with model group. Hippocampal homogenates showed enhanced cholinesterase activity, and dose-dependent increases in acetylcholine content and decreases in choline content following eleutheroside B or E treatment, similar to those seen in the Huperzine A group. These findings indicate that eleutheroside B or E improves learning and memory in aged rats. These effects of eleutheroside B or E may be mediated by activation of cholinesterase or enhanced reuse of choline to accelerate the synthesis of acetylcholine in hippocampal neurons.
