[ Objective ] The purpose was to provide a reference for crossbreeding of grass carp. [ Method] We conducted hybridization between Ctenopharyngodon ideUus （ ♀ ） and Elopichthys bambusa ♂ ） by artificial methods. ...[ Objective ] The purpose was to provide a reference for crossbreeding of grass carp. [ Method] We conducted hybridization between Ctenopharyngodon ideUus （ ♀ ） and Elopichthys bambusa ♂ ） by artificial methods. The process of embryo and postembryonic development were observed and recorded. [ Result ] The fertilization rate, hatching rate and survival rate of the hybrid F1 were （75.8 ± 6. 2）%, （41.9 ± 8.2）% and （9.3± 3.7）%, respectively. At the water temperature of 20.1 -21.6℃, the larvae was hatched for about 34 h and 25 rain after fertilization. The whole length of newly-hatched larvae of the hybrid Fl was （5.8 ± 0.12） mm. The larvae could feed rotifer and unicellular algae after 3 or 4 days hatching. Postembryonic development of the hybrid at the formation of scales, lasted for 65 days when the young was（74.0 ± 2.1 ） mm in whole length. [ Conclusion] The embryonic development of hybrid F1 was intermediate to their parents, the form of the hybrid F1 was similar to that of their female line of grass carp. The growth rate of larva was faster than that of grass carp and similar to that of their paternal fish.展开更多
[ Objective] This study aimed to investigate the biochemical and genetic characteristics of F1 hybrids of Ctenopharyngodon idellus ♀× Elopichthys bambusa ♂ - [ Method ] By using vertical polyacrylamide gel elec...[ Objective] This study aimed to investigate the biochemical and genetic characteristics of F1 hybrids of Ctenopharyngodon idellus ♀× Elopichthys bambusa ♂ - [ Method ] By using vertical polyacrylamide gel electrophoresis technology, three isozymes （LDH, EST, MDH） in nine tissues （heart, brain, eyes, liver, kidney, spleen, fin, muscle, plasma） of F1 hybrids of C. ideUus and parents were compared. [Result] Three isozymes in F1 hybrids of ♀× E. bambusa ♀ were analyzed; differences in isozyme and proteins between F1 hybrids idellus ♀× E. bambusa ♀ exhibited varying degrees of tissue-specificity; isozymes and proteins in F, hybrids were signiticandy different from the parents. [ Conclusion] These nine differences could be used as indicators to identify F1 hybrids and parents.展开更多
Plant of Bambusa vulgaris is used in traditional medicine practice for the management of many pathologies including hypertension. The effect of the aqueous extract of the leaves of Bambusa vulgaris on blood pressure w...Plant of Bambusa vulgaris is used in traditional medicine practice for the management of many pathologies including hypertension. The effect of the aqueous extract of the leaves of Bambusa vulgaris on blood pressure was evaluated in normotensive male rabbits. Blood pressure was measured by the invasive method. Thus, aqueous extract of Bambusa vulgaris leaves had been injected intravenously at doses ranging from 1 mg/kg to 30 mg/kg in rabbits anesthetized with thiopental. The effects of this extract on blood pressure were also evaluated in rabbits having previously received different doses of atropine. The aqueous extract of Bambusa vulgaris leaves induced a dose-dependent hypotension which is not canceled in the presence of atropine. The results obtained show that the extract has blood pressure lowering effect which may be mediated by muscarinic-type cholinergic receptors.展开更多
基金Supported by Wuhan Science and Technology Plan Projects(2013021001010464)
文摘[ Objective ] The purpose was to provide a reference for crossbreeding of grass carp. [ Method] We conducted hybridization between Ctenopharyngodon ideUus （ ♀ ） and Elopichthys bambusa ♂ ） by artificial methods. The process of embryo and postembryonic development were observed and recorded. [ Result ] The fertilization rate, hatching rate and survival rate of the hybrid F1 were （75.8 ± 6. 2）%, （41.9 ± 8.2）% and （9.3± 3.7）%, respectively. At the water temperature of 20.1 -21.6℃, the larvae was hatched for about 34 h and 25 rain after fertilization. The whole length of newly-hatched larvae of the hybrid Fl was （5.8 ± 0.12） mm. The larvae could feed rotifer and unicellular algae after 3 or 4 days hatching. Postembryonic development of the hybrid at the formation of scales, lasted for 65 days when the young was（74.0 ± 2.1 ） mm in whole length. [ Conclusion] The embryonic development of hybrid F1 was intermediate to their parents, the form of the hybrid F1 was similar to that of their female line of grass carp. The growth rate of larva was faster than that of grass carp and similar to that of their paternal fish.
基金Supported by Project of Project of Science and Technology of Wuhan City(2013021001010464)
文摘[ Objective] This study aimed to investigate the biochemical and genetic characteristics of F1 hybrids of Ctenopharyngodon idellus ♀× Elopichthys bambusa ♂ - [ Method ] By using vertical polyacrylamide gel electrophoresis technology, three isozymes （LDH, EST, MDH） in nine tissues （heart, brain, eyes, liver, kidney, spleen, fin, muscle, plasma） of F1 hybrids of C. ideUus and parents were compared. [Result] Three isozymes in F1 hybrids of ♀× E. bambusa ♀ were analyzed; differences in isozyme and proteins between F1 hybrids idellus ♀× E. bambusa ♀ exhibited varying degrees of tissue-specificity; isozymes and proteins in F, hybrids were signiticandy different from the parents. [ Conclusion] These nine differences could be used as indicators to identify F1 hybrids and parents.
文摘Plant of Bambusa vulgaris is used in traditional medicine practice for the management of many pathologies including hypertension. The effect of the aqueous extract of the leaves of Bambusa vulgaris on blood pressure was evaluated in normotensive male rabbits. Blood pressure was measured by the invasive method. Thus, aqueous extract of Bambusa vulgaris leaves had been injected intravenously at doses ranging from 1 mg/kg to 30 mg/kg in rabbits anesthetized with thiopental. The effects of this extract on blood pressure were also evaluated in rabbits having previously received different doses of atropine. The aqueous extract of Bambusa vulgaris leaves induced a dose-dependent hypotension which is not canceled in the presence of atropine. The results obtained show that the extract has blood pressure lowering effect which may be mediated by muscarinic-type cholinergic receptors.
基金supported by the National Key Research&Development Program of China(Grant No.2016YFD0600901)the National Natural Science Foundation of China(Grant No.31000294)the Priority Academic Program Development of Jiangsu Higher Education Institutions
