In this study, the comparison of Elsholtzia ciliata volatile compounds from the stem (with leaf) and flower was acquired. The volatile compounds of these two parts from Elsholtzia ciliata aerial parts were respectivel...In this study, the comparison of Elsholtzia ciliata volatile compounds from the stem (with leaf) and flower was acquired. The volatile compounds of these two parts from Elsholtzia ciliata aerial parts were respectively analyzed by a rapid and convenient static headspace injection technique coupled with gas chromatography-mass spectrometry and the relative contents of each constituent between the two different parts were determined by peak area normalization. 61 compounds were identified in the stem (with leaf) and flower, among which 39 components simultaneously exist. 47 and 53 compounds were separated and identified in the volatile compounds from the different parts of Elsholtzia ciliata, respectively. There were differences among some volatile constituents of the two parts, but the main constituents were all Elsholtzia ketone, caryophyllene, 3-octanol and Dehydroelsholtzia ketone. The most abundant components in the stem (with leaf) included Elsholtzia ketone (84.20%), caryophyllene (4.3%) and 3-octanol (3.11%), while Elsholtzia ketone (88.03%), caryophyllene (3.33%) and 3-octanol (1.53%) were the main components in the flower. 8 single constituents were identified in the stem (with leaf) volatiles including 3-heptanone and linalool, while 14 constituents only including 2-methylbutanoic acid and Perillene have been found in the flower volatiles. Elsholtzia ketone was the main compound, and its concentration was significantly higher than other substances (>80% of the total oils in Elsholtzia ciliata). The study provided an important scientific base for the further utilization of Elsholtzia ciliata resources and may be helpful for systematically understanding the constituents of volatile compounds of Elsholtzia ciliata.展开更多
目的分析香薷Elsholtzia ciliata(Thunb.)Hyland.、野草香Elsholtzia cypriani(Pavol.)C.Y.Wu et S.Chow挥发油,并评价其生物活性。方法水蒸气蒸馏法提取样品花、茎叶中的挥发油,GC-MS法进行分析,DPPH、ABTS法分别测定抗氧化活性,纸片...目的分析香薷Elsholtzia ciliata(Thunb.)Hyland.、野草香Elsholtzia cypriani(Pavol.)C.Y.Wu et S.Chow挥发油,并评价其生物活性。方法水蒸气蒸馏法提取样品花、茎叶中的挥发油,GC-MS法进行分析,DPPH、ABTS法分别测定抗氧化活性,纸片琼脂扩散法测定对大肠杆菌、金黄色葡萄球菌、伤寒沙门氏菌、肺炎克雷伯氏菌、铜绿假单胞杆菌的抑制作用。结果从中共鉴定出59种成分,其中香薷以柠檬烯、芳樟醇、β-蛇床烯为主,野草香以环氧玫瑰呋喃、百里醌、β-石竹烯为主。香薷花、茎叶挥发油的抗氧化活性相近,野草香茎叶挥发油的抗氧化活性略强于花。野草香花挥发油可显著抑制金黄色葡萄球菌,对伤寒沙门氏菌和肺炎克雷伯氏菌也有一定抑制作用。结论香薷、野草香挥发油主要成分差异明显,并均具有一定生物活性,有着较好的开发价值和前景。展开更多
Objective To compare the chemical constituents in the essential oils from the leaves, flowers, and seeds of Elsholtzia ciliata and their antimicrobial activities. Methods The chemical constituents in essential oils we...Objective To compare the chemical constituents in the essential oils from the leaves, flowers, and seeds of Elsholtzia ciliata and their antimicrobial activities. Methods The chemical constituents in essential oils were extracted by the hydro-distillation method and analyzed by GC-MS. The chemical constituents in essential oils were identified on the basis of comparison on their retention indices and MS spectrum with published data. Moreover, the antimicrobial activities of the chemical constituents in the oils against the growth of six bacteria strains and one pathogenic yeast strain were evaluated by using minimum inhibitory concentration and minimum bactericidal concentration methods. Results A total of 58 compounds were identified, while compounds 31, 35, and 36 were identified in the essential oils from the leaves, flowers, and seeds, respectively. Fifteen compounds were identified as shared constituents in the leaves, flowers, and seeds. The chemical constituents in the essential oils showed the inhibitory activities against the six bacteria strains and the yeast strain. Conclusion The major constituents are different in the essential oils of the leaves, flowers, and seeds. The major chemical constituents in the essential oils are monoterpenoids and sesquiterpenoids. And the chemical constituents in the essential oils obtained from the leaves show higher inhibitory activities especially against Bacillus subtillis CMCC63501 and Escherichia coli ATCC25922.展开更多
文摘In this study, the comparison of Elsholtzia ciliata volatile compounds from the stem (with leaf) and flower was acquired. The volatile compounds of these two parts from Elsholtzia ciliata aerial parts were respectively analyzed by a rapid and convenient static headspace injection technique coupled with gas chromatography-mass spectrometry and the relative contents of each constituent between the two different parts were determined by peak area normalization. 61 compounds were identified in the stem (with leaf) and flower, among which 39 components simultaneously exist. 47 and 53 compounds were separated and identified in the volatile compounds from the different parts of Elsholtzia ciliata, respectively. There were differences among some volatile constituents of the two parts, but the main constituents were all Elsholtzia ketone, caryophyllene, 3-octanol and Dehydroelsholtzia ketone. The most abundant components in the stem (with leaf) included Elsholtzia ketone (84.20%), caryophyllene (4.3%) and 3-octanol (3.11%), while Elsholtzia ketone (88.03%), caryophyllene (3.33%) and 3-octanol (1.53%) were the main components in the flower. 8 single constituents were identified in the stem (with leaf) volatiles including 3-heptanone and linalool, while 14 constituents only including 2-methylbutanoic acid and Perillene have been found in the flower volatiles. Elsholtzia ketone was the main compound, and its concentration was significantly higher than other substances (>80% of the total oils in Elsholtzia ciliata). The study provided an important scientific base for the further utilization of Elsholtzia ciliata resources and may be helpful for systematically understanding the constituents of volatile compounds of Elsholtzia ciliata.
文摘目的分析香薷Elsholtzia ciliata(Thunb.)Hyland.、野草香Elsholtzia cypriani(Pavol.)C.Y.Wu et S.Chow挥发油,并评价其生物活性。方法水蒸气蒸馏法提取样品花、茎叶中的挥发油,GC-MS法进行分析,DPPH、ABTS法分别测定抗氧化活性,纸片琼脂扩散法测定对大肠杆菌、金黄色葡萄球菌、伤寒沙门氏菌、肺炎克雷伯氏菌、铜绿假单胞杆菌的抑制作用。结果从中共鉴定出59种成分,其中香薷以柠檬烯、芳樟醇、β-蛇床烯为主,野草香以环氧玫瑰呋喃、百里醌、β-石竹烯为主。香薷花、茎叶挥发油的抗氧化活性相近,野草香茎叶挥发油的抗氧化活性略强于花。野草香花挥发油可显著抑制金黄色葡萄球菌,对伤寒沙门氏菌和肺炎克雷伯氏菌也有一定抑制作用。结论香薷、野草香挥发油主要成分差异明显,并均具有一定生物活性,有着较好的开发价值和前景。
基金The Society Progress Project Supported by Shaanxi Province of China(2012K19-03-03)Shaanxi Provincial Education Department of China(2010JK471)
文摘Objective To compare the chemical constituents in the essential oils from the leaves, flowers, and seeds of Elsholtzia ciliata and their antimicrobial activities. Methods The chemical constituents in essential oils were extracted by the hydro-distillation method and analyzed by GC-MS. The chemical constituents in essential oils were identified on the basis of comparison on their retention indices and MS spectrum with published data. Moreover, the antimicrobial activities of the chemical constituents in the oils against the growth of six bacteria strains and one pathogenic yeast strain were evaluated by using minimum inhibitory concentration and minimum bactericidal concentration methods. Results A total of 58 compounds were identified, while compounds 31, 35, and 36 were identified in the essential oils from the leaves, flowers, and seeds, respectively. Fifteen compounds were identified as shared constituents in the leaves, flowers, and seeds. The chemical constituents in the essential oils showed the inhibitory activities against the six bacteria strains and the yeast strain. Conclusion The major constituents are different in the essential oils of the leaves, flowers, and seeds. The major chemical constituents in the essential oils are monoterpenoids and sesquiterpenoids. And the chemical constituents in the essential oils obtained from the leaves show higher inhibitory activities especially against Bacillus subtillis CMCC63501 and Escherichia coli ATCC25922.
