Experimental Study of Emilia Sonchifolia Combined with Coptidis Rhizoma for the Prevention and Treatment of Oral Ulcer

Objective: This paper aims to investigate the therapeutic effect of the combination of Emilia Sonchifolia and Coptidis Rhizoma on oral ulcer rats. Methods: 36 SD rats of half male and half female were kept for 7 days, and 6 rats among them were selected as normal group by random sampling method, and the rest rats were randomly divided into model group, positive control group, Emilia Sonchifolia group, Coptidis Rhizoma group and combined group after the establishment of oral ulcer model. The normal group and model group were given blank film, the positive control group was given Guilin Watermelon Frost, and the Emilia Sonchifolia group, Coptidis Rhizoma group and combined group were given the corresponding oral film, which was administered to the ulcer for 7 days continuously, 2 times per day. The healing of oral ulcer was assessed at the end of the last day of administration, and the ulcer area was calculated on the 1st, 3rd, 5th and 7th days after successful modeling, and the serum levels of IL-2 and TNF-α in rats were detected by Emilia Sonchifolia. Results: The grading of ulcer healing in the positive control group and the combined group was better than the other groups, and the difference was statistically significant when compared with the model group (p Coptidis Rhizoma group, Emilia Sonchifolia group, combined group and positive control group was smaller than that in the model group, and the difference was statistically significant (p Emilia Sonchifolia group, Coptidis Rhizoma group and combined group were all effective in reducing the area of oral ulcer, among which the combined group was more effective. The level of pro-inflammatory factor TNF-α was reduced and the level of anti-inflammatory factor IL-2 was increased in the Emilia Sonchifolia group, Coptidis Rhizoma group and combined group, and the difference was statistically significant (p Conclusion: The combination of Coptidis Rhizoma and Emilia Sonchifolia is effective in the treatment of oral ulcer in rats, and the effect of the combination is better than that of the drug alone.

Optimization of the Ultrasonic-assisted Extraction Process for Protocatechuic Acid from Emilia sonchifolia DC by Response Surface Methodology

[Objectives] This study was conducted to optimize the extraction process of protocatechuic acid from Emilia sonchifolia DC. [Methods] The optimal extraction conditions were determined by single factor,response surface analysis and variance analysis,and the content of protocatechuic acid was determined by HPLC. [Results] The protocatechuic acid standard curve equation was: y = 1 435 x + 8 403,R^2= 0. 999 8,indicating a good linear relationship. The optimal extraction conditions were as follows: a temperature at 80 ℃,an extraction time of 1 h,a material-to-liquid ratio at 1:10 and an ultrasonic power of 600 W,and the content of protocatechuic acid extracted was 1. 93 mg/g. The method showed a RSD of 0. 41%,less than 2%,and the detection limit was 0. 0000047261 g/ml.The experimental sample X1 was the low-level 0. 1 mg/ml standard solution,which showed recovery of protocatechuic acid between 100.8% and 105.2%,with a RSD of 0. 013%;and the sample X2 was the high-level 1. 0 mg/ml standard solution,which exhibited recovery between 100. 6% and 102. 2%,with a RSD of 0.076%. Thus,the recovery was high,and the requirements of the performance index were met. [Conclusions] The detection method is stable and reliable and can produce satisfactory results.

Computational Stimulation and Experimental Study on Corrosion Inhibition Qualities of <i>Emilia sonchifolia</i>Leaf Extract for Copper (CU131729) in Hydrochloric Acid

Copper corrosion inhibition by Emilia sonchifolia (ES) leaf extract has been studied in 2 M hydrochloric acid solution using electrochemical measurement, energy dispersive X-ray emission spectroscopy and surface examination techniques. Computational simulations were adopted to describe probable reactiveness of individual ES leaf constituents and mechanism of interaction with copper crystal. Results obtained from potentiodynamic polarization revealed a shift in corrosion potentials of copper (CU131729) to more positive potentials in the presence of ES leaf extract with increased effect as inhibitor concentration increased and also a decrease in both current densities suggesting a mixed type inhibitor characteristics with pronounced anodic protection. Surface elemental characterization revealed presence of the inhibitor species in the corrosion products formed on corroded CU131729 surface confirming the formation of complex chelating ligands through interactions with the metal surface. Quantum chemical calculations and molecular dynamics simulations were employed to theoretically analyse the interactions of individual ES leaf constituent with copper surface at the molecular level and obtained results revealed strong and spontaneous adsorption with high binding energies which affirms observed quality inhibition action. Quantum chemical descriptors such as energy of HOMO and LUMO, energy gap, number of transferred electrons, global hardness and softness, electrophilicity, and interaction energy were computed and discussed.

Effect of Emilia sonchifolia(Linn.)DC on alcohol- induced oxidative stress in pancreas of male albino rats

Objective:To explore the efficacy of n-hexane extract of Emilia sonchifolia(E.sonchifobia) against ethanol induced pancreatic dysfunction in the young Wistar albino rats.Methods:The rats were divided into four groups.Control rats in group I received distilled water orally,groupⅡreceived oral administration of 20%(w/v) ethanol dissolved in drinking water,groupⅢreceived oral administration of 20%(w/v) ethanol in distilled water+n-hexane extract of E.sonchifolia (250 mg/kg body weight),and groupⅣreceived oral administration of n-hexane extract of E.sonchifolia(250 mg/kg body weight) alone.Liver marker enzymes aspartate aminotransferase (AST),alanine aminotransferase(ALT),pancreatic enzymatic antioxidants superoxide dismutase. lipid peroxidation,catalase,glutathione peroxidase,non-enzymatic antioxidants glutathione and vitamin C were measured and compared.Results:Administration of 20%ethanol lor 16 weeks significantly increased the liver marker enzymes AST,ALT(P【0.05).reduced the pancreatic enzymatic antioxidants superoxide dismutase.lipid peroxidation,catalase,glutathione peroxidase,glutathione and vitamin C(P【0.05).Histopathological examination showed that the ethanol provoked the oxidative stress which was demonstrated as pancreatic necrosis and oedema.Simultaneous administration of n-hexane extract of E.sonchifolia(250 mg/kg body weight) protected the pancreas against the damage induced by ethanol which was confirmed by the histopathological studies and the normalization of biochemical parameters.Conclusions: Thus n-hexane extract of E.sonchifolia shows a promise in therapeutic use in alcohol induced oxidative stress.

不同烹饪方式对一点红抗氧化活性的影响

为了探究不同烹饪方式对一点红抗氧化活性的影响,分别采用9种不同烹饪方式(漂烫0.5 min、漂烫1.5 min、漂烫2.5 min、煮制5 min、煮制10 min、煮制15 min、油炒2 min、油炒4 min、油炒6 min)对其处理后测定总多酚含量、总黄酮含量、DPPH清除率、总抗氧化能力、维生素C含量、叶绿体色素含量等指标。结果表明,总多酚含量最高、总抗氧化能力最强、叶绿素含量、类胡萝卜素含量最高的烹饪方式是油炒6 min;总黄酮含量、DPPH清除率最高的是漂烫2.5 min;维生素C含量最高的是漂烫0.5 min。主成分综合分析结果显示,综合得分排名最高的是油炒6 min。因此,油炒6 min是保留一点红最高抗氧化活性的最佳烹饪方式。

一点红黄酮成分的分析及含量测定

对一点红中黄酮类成分进行了定性分析和定量测定,结果表明,在一点红中含有黄酮、黄酮醇、二氢黄酮、二氢黄酮醇等多种黄酮类化合物,并以芦丁为标样,用分光光度法测出总黄酮含量为4.02%.

一点红黄酮类化合物分离及其抗菌性能研究

本实验采用70%的乙醇水溶液回流提取一点红中的黄酮类化合物,然后通过石油醚萃取除掉其中色素等杂质。提取物进一步通过聚酰胺薄板进行薄层色谱分析[展层剂为正丁醇:水:醋(10:5:1)],且通过聚酰胺柱进行分离纯化(洗脱液为70%乙醇水溶液)。薄层色谱分析结果(以芦丁标样为对照)以及相关的黄酮化学检识实验结果表明,一点红黄酮类化合物具有与芦丁成分相似的黄酮类化合物和较高的纯度,对金黄色葡萄球菌具有较强的抑菌作用,对大肠杆菌及枯草杆菌的抑菌效果次之。

一点红总黄酮的提取及对羟自由基的清除作用研究

目的探讨一点红总黄酮的提取、鉴别及对羟自由基清除作用,以充分利用一点红植物资源,避免资源的浪费。方法采用超声波乙醇浸提法从一点红中提取黄酮类物质,对所提取的黄酮类物质进行验证,并用分光光度法测定含量,用一点红总黄酮对羟自由基清除作用进行试验。结果测得样品中总黄酮的含量C=0.816 8 mg/ml,回收率为100%,其纯度和产率均较高。结论该方法采用全物理过程,无任何污染,是提取一点红黄酮类物质的有效途径。一点红总黄酮提取液对Fenton体系产生的.OH自由基有很好的清除作用。

广西一点红总生物碱的提取和抗菌活性研究

目的考察广西一点红总生物碱的分离提纯及其抗菌活性。方法通过95％乙醇回流提取一点红药材中的生物碱类物质，利用酸水提取法及有机溶剂萃取对其进行分离纯化，同时用化学检识法与薄层色谱法进行定性鉴别。采用滤纸片法研究一点红生物碱类物质的抗菌活性。结果一点红生物碱浓度在600～800mg／ml时对金黄色葡萄球菌、大肠杆菌及枯草杆菌中度敏感，随着药物（生物碱）浓度的增大，抗菌作用越明显。结论说明一点红生物碱类物质对供试菌有一定的抑菌活性。

一点红黄酮分离及抗氧化研究

研究一点红黄酮的分离提纯及其清除1,1-二苯基-2-苦基苯肼自由基(DPPH·)和羟基自由基(·OH)作用。经乙醇回流提取、聚酰胺柱层析分离提纯得到一点红黄酮;采用薄层层析对其进行定性分析;采用紫外-可见分光光度法测定其清除DPPH·和·OH的能力。结果表明,一点红黄酮清除DPPH·和·OH的IC50分别是4.8×10-3、0.53mg/ml,说明它具有清除DPPH·和·OH的作用。

广西传统中药一点红总黄酮含量分析

目的建立一点红总黄酮的含量测定方法，考察一点红原药材质量的优劣。方法利用正交设计法考察最佳提取条件，采用紫外-可见分光光度法，以芦丁为对照品，于510nm波长处测定总黄酮含量。结果芦丁在0．0312～0．0832mg／ml范围内呈良好线性关系（R^2=0．9984），平均加样回收率为96．8％，RSD=1．21％（n=6）。3批样品总黄酮平均含量为9．918mg／g。结论该法重复性较好，对仪器要求不高，准确可靠，可用于一点红质量控制。

一点红化学成分的研究

从一点红全草中分得5个化合物,经化学和光谱方法鉴定为simiral,β-谷甾醇,豆甾醇,棕榈酸和蜂蜜酸。

一点红不同营养器官中总黄酮和总酚含量测定

目的:建立一点红不同营养器官中总黄酮和总酚含量测定方法。方法:以芦丁为对照品,采用NaNO2-Al(NO3)3-NaOH比色法测定了总黄酮含量;以没食子酸为对照品,采用Folin-Ciocalteu比色法测定了总酚含量。结果:芦丁浓度在0.01～0.08mg/ml范围内与吸光度呈良好线性关系,平均加样回收率为100.30%,RSD=1.72%,一点红叶、茎、根中总黄酮含量分别为2.54%、1.78%、1.27%;没食子酸浓度在0.002～0.010mg/ml范围内与吸光度呈良好线性关系,平均加样回收率为99.86%,RSD=1.92%,一点红叶、茎、根中总酚含量分别为21.28%、12.77%、8.51%。结论:这2种方法灵敏,准确性强,精密度高,重复性好,可以用作一点红中总黄酮和总酚含量的测定。

不同采收期广西一点红总生物碱含量分析

目的:建立一点红总生物碱的含量测定方法,测定不同采收期一点红中总生物碱含量,考察一点红原药材质量的优劣。方法:利用单因素法考察最佳提取条件,采用紫外可见分光光度法,以苦参碱为对照品,于414nm波长处测定生物碱含量。结果:苦参碱在0.0021～0.0294mg/ml范围内呈良好线性关系(R2=0.9997),平均加样回收率为97.6%,RSD为0.447%(n=6)。样品的总生物碱含量在4.31～13.99mg/g之间,八月为最佳采收期,其样品中总生物碱含量最高。结论:本法测定,重复性较好,对仪器要求不高,准确可靠,可用于一点红质量控制。

不同产地与不同采收期一点红总黄酮含量分析

本实验通过测定不同产地、不同采收期一点红中总黄酮含量,建立了一种以总黄酮含量变化为基础的一点红质量测定方法,确定了一点红最佳采收期。利用单因素与正交试验设计法考察最佳提取条件,采用紫外-可见分光光度法,以芦丁为对照品,于510nm波长处测定总黄酮含量。芦丁在0.0104～0.8160mg/ml范围内呈良好线性关系(R2=0.9994),平均加样回收率为99.2%,RSD为1.069%(n=6)。样品的总黄酮含量在6.30～14.16mg/g之间,结果表明,广东韶关的一点红总黄酮含量较高,一点红最佳采收期为九月份。

一点红的人工栽培试验

目的探讨人工栽培一点红的可行性,旨在实现规范化种植。方法用种子繁殖试种。结果一年能种植两造,以早造产量较高;可以大田直播,也可以育苗移栽;适宜的种植密度为行株距25cm×20cm。结论人工栽培一点红是可行的。

一点红不同生长期氨基酸含量分析

[目的]分析一点红营养生长期和花期的氨基酸含量。[方法]利用日立L-8800氨基酸自动分析仪测定氨基酸含量。[结果]营养生长期和花期的一点红分别含16种氨基酸,氨基酸总量分别达13.892%、13.746%;其中7种人体必需氨基酸占氨基酸总量分别为43.968%、44.507%;人体必需氨基酸总质量分数与非必需氨基酸总质量分数比分别为0.785、0.802;它们分别含有9种药效氨基酸,分别占氨基酸总量的60.834%、61.851%。[结论]一点红具有较高的营养价值和药用价值,值得大力开发和利用。

一点红不同营养器官中鞣质和绿原酸含量测定

目的:建立一点红不同营养器官中鞣质和绿原酸含量测定方法。方法:以没食子酸为对照品,采用磷钼钨酸-干酪素比色法测定了鞣质含量;以绿原酸为对照品,采用高效液相色谱法测定了绿原酸含量。结果:没食子酸浓度在2～10mg·L-1范围内,与吸光度呈良好线性关系,平均加样回收率为100.32%,RSD=1.45%,一点红叶、茎、根中鞣质含量分别为0.25%、0.15%、0.22%;绿原酸含量在0.0356～0.5340μg范围内与峰面积积分值呈良好线性关系,平均加样回收率为99.37%,RSD=1.52%,一点红叶、茎、根中绿原酸含量分别为1.28%、1.14%、0.91%。结论:这两种方法灵敏,准确性强,精密度高,重复性好,适用于一点红中鞣质和绿原酸含量的测定。

一点红挥发油化学成分的分析

目的:研究一点红挥发油的化学成分。方法:采用水蒸气蒸馏法提取一点红挥发油,用气相色谱-质谱联用法,分离分析其成分及相对含量。结果:共检出80个化合物,鉴定其中28个化合物,占挥发油总量的76.97%。结论:一点红挥发油的主要成分为Oplopenone和石竹烯氧化物。

一点红不同溶剂浸提物的抑菌作用

[目的]研究一点红不同溶剂浸提物的抑菌性。[方法]采用甲醇、乙醇、丙酮乙酸乙酯及乙醚5种溶剂提取一点红,并且采用用滤纸片扩散法研究一点红不同溶剂提取物对金黄色葡萄球菌、大肠杆菌的抑菌作用。[结果]提取效率顺序为甲醇>乙醇>丙酮>乙醚>乙酸乙酯,而一点红提取物对金黄色葡萄球菌和大肠杆菌的抑菌作用强弱顺序为乙醚>乙醇>甲醇>丙酮>乙酸乙酯。[结论]乙醇浸提一点红的效率较高,抑菌作用较好。