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Adams-Oliver综合征5型合并IgA肾病1例并文献复习
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作者 史慧远 宋纯东 张博 《疑难病杂志》 CAS 2024年第8期999-1001,共3页
报道1例Adams-Oliver综合征5型合并IgA肾病患儿的临床资料,并进行文献复习。
关键词 Adams-oliver综合征5型 NOTCH1基因 IGA肾病 诊断 治疗
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Olive Leaf Disease Detection via Wavelet Transform and Feature Fusion of Pre-Trained Deep Learning Models
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作者 Mahmood A.Mahmood Khalaf Alsalem 《Computers, Materials & Continua》 SCIE EI 2024年第3期3431-3448,共18页
Olive trees are susceptible to a variety of diseases that can cause significant crop damage and economic losses.Early detection of these diseases is essential for effective management.We propose a novel transformed wa... Olive trees are susceptible to a variety of diseases that can cause significant crop damage and economic losses.Early detection of these diseases is essential for effective management.We propose a novel transformed wavelet,feature-fused,pre-trained deep learning model for detecting olive leaf diseases.The proposed model combines wavelet transforms with pre-trained deep-learning models to extract discriminative features from olive leaf images.The model has four main phases:preprocessing using data augmentation,three-level wavelet transformation,learning using pre-trained deep learning models,and a fused deep learning model.In the preprocessing phase,the image dataset is augmented using techniques such as resizing,rescaling,flipping,rotation,zooming,and contrasting.In wavelet transformation,the augmented images are decomposed into three frequency levels.Three pre-trained deep learning models,EfficientNet-B7,DenseNet-201,and ResNet-152-V2,are used in the learning phase.The models were trained using the approximate images of the third-level sub-band of the wavelet transform.In the fused phase,the fused model consists of a merge layer,three dense layers,and two dropout layers.The proposed model was evaluated using a dataset of images of healthy and infected olive leaves.It achieved an accuracy of 99.72%in the diagnosis of olive leaf diseases,which exceeds the accuracy of other methods reported in the literature.This finding suggests that our proposed method is a promising tool for the early detection of olive leaf diseases. 展开更多
关键词 olive leaf diseases wavelet transform deep learning feature fusion
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Eco-physiological characteristics of Tetracentron sinense Oliv.saplings in response to different light intensities
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作者 Rong Wang Xueheng Lu +4 位作者 Hongyan Han Xuemei Zhang Yonghong Ma Qinsong Liu Xiaohong Gan 《Journal of Forestry Research》 SCIE EI CAS CSCD 2024年第3期29-44,共16页
The regeneration of Tetracentron sinense Oliv.is poor in the understory and in open areas due to the charac-teristics of natural regeneration of the species on forest edges and in gaps.It is unclear whether different ... The regeneration of Tetracentron sinense Oliv.is poor in the understory and in open areas due to the charac-teristics of natural regeneration of the species on forest edges and in gaps.It is unclear whether different light intensities in various habitats affect eco-physiological characteristics of saplings and their natural regeneration.In this study,the light intensity in T.sinense habitats was simulated by artificial shading(L1:100%NS(natural sunlight)in the open;L2:50%NS in a forest gap or edge;L3:10%NS in understory)to investigate differences in morphology,leaf structure,physiology,and photosynthesis of 2-year-old sap-lings,and to analyze the mechanism of light intensity on sapling establishment.Significant differences were observed in morphology(including leaf area,and specific leaf area)under different light intensities.Compared to L1 and L3,chloroplast structure in L2 was intact.With increasing time,superoxide dismutase(SOD)and catalase(CAT)activities in L2 became gradually higher than under the other light intensities,while malondialdehyde(MDA)content was opposite.Shading decreased osmoregulation substance contents of leaves but increased chlorophyll.The results suggest that light intensities significantly affect the eco-physiological characteristics of T.sinense saplings and they would respond most favorably at intermediate levels of light by optimizing eco-physiological characteristics.Therefore,50%natural sunlight should be created to promote saplings establishment and population recovery of T.sinense during in situ conservation,including sowing mature seeds in forest edges or gaps and providing appropriate shade protection for seedlings and saplings in the open. 展开更多
关键词 Chloroplast ultrastructure Eco-physiological characteristics Light intensities Sapling establishment Tetracentron sinense oliv
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Protective effects of oleic acid and polyphenols in extra virgin olive oil on cardiovascular diseases
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作者 Yan Lu Jun Zhao +6 位作者 Qiqi Xin Rong Yuan Yu Miao Manli Yang Hui Mo Keji Chen Weihong Cong 《Food Science and Human Wellness》 SCIE CSCD 2024年第2期529-540,共12页
The Mediterranean diet has long been recognized as one of the most effective ways to prevent and improve cardiovascular disease.Extra virgin olive oil(EVOO)is the typical sources of fat in the Mediterranean diet which... The Mediterranean diet has long been recognized as one of the most effective ways to prevent and improve cardiovascular disease.Extra virgin olive oil(EVOO)is the typical sources of fat in the Mediterranean diet which have been shown to have noteworthy nutritional value and positive impact on human health.It is worth noting that EVOO owes its superior nutritional value to its bioactive composition.The main component of EVOO is monounsaturated fatty acids(MUFAs)in the form of oleic acid.Oleic acid accounts for up to 70%-80%of EVOO.Secondly,EVOO contains approximately more than 30 phenolic compounds,of which HT is essential for the protection against cardiovascular diseases.In this review,we focused on the potential mechanisms of oleic acid and polyphenols combat cardiovascular diseases risk in terms of oxidative stress,inflammation,blood pressure,endothelial function and cholesterol.This review might provide a reference for the studies on cardiovascular protective effects of EVOO. 展开更多
关键词 Cardiovascular diseases Extra virgin olive oil Oleic acid POLYPHENOLS
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Thermal Decomposition of Olive-Solid Waste by TGA: Characterization and Devolatilization Kinetics under Nitrogen and Oxygen Atmospheres
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作者 Yahya H. Khraisha 《Journal of Power and Energy Engineering》 2024年第3期31-47,共17页
Despite the fact that a few countries in the Mediterranean and the Middle East have limited crude oil reserves, they have abundant biomass feedstocks. For instance, Jordan relies heavily on the importation of natural ... Despite the fact that a few countries in the Mediterranean and the Middle East have limited crude oil reserves, they have abundant biomass feedstocks. For instance, Jordan relies heavily on the importation of natural gas and crude oil for its energy needs;but, by applying thermochemical conversion techniques, leftover olive oil can be used to replace these energy sources. Understanding the chemical, physical, and thermal characteristics of raw materials is essential to obtaining the most out of these conversion processes. Thermogravimetric analysis was used in this study to examine the thermal behavior of olive-solid residue (kernel) at three different heating rates (5, 20 and 40 C/min) in nitrogen and oxygen atmospheres. The initial degradation temperature, the residual weight at 500 and 700˚C and the thermal degradation rate during the devolatilization stage (below 400˚C) were all determined. It was found that in N<sub>2</sub> and O<sub>2</sub> atmospheres, both the initial degradation temperature and the degradation rate increase with increasing heating rates. As heating rates increase in the N<sub>2</sub> atmosphere, the residual weight at 500 or 700˚C decreases slightly, but at low heating rates compared to high heating rates in the O<sub>2</sub> atmosphere, it decreases significantly. This suggests that a longer lignin oxidation process is better than a shorter one. Coats and Redfern approach was used to identify the mechanism and activation energy for the devolatilization stage of pyrolysis and oxidation reactions. The process mechanism analysis revealed that the model of first-order and second-order reactions may adequately describe the mechanism of heat degradation of the devolatilization step of olive-solid waste for pyrolysis and oxidation processes, respectively. 展开更多
关键词 Biomass olive-Solid Waste THERMOGRAVIMETRY Pyrolysis Oxidation Heating Rates Kinetics
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Integrated Use of Organic and Bio-fertilizers to Improve Yield and Fruit Quality of Olives Grown in Low Fertility Sandy Soil in an Arid Environment 被引量:1
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作者 Bassam F.Alowaiesh M.M.Gad Mohamed Saleh M.Ali 《Phyton-International Journal of Experimental Botany》 SCIE 2023年第6期1813-1829,共17页
Olive productivity should be improved through stimulating nutrition,particularly under poor fertility soils.Consequently,the objective of this study was to assess the efficacy of applying organic and bio-fertilizers o... Olive productivity should be improved through stimulating nutrition,particularly under poor fertility soils.Consequently,the objective of this study was to assess the efficacy of applying organic and bio-fertilizers on the physiological growth,yield and fruit quality of olive trees under newly reclaimed poor-fertility sandy soil in an arid environment.During a field experiment carried out at El-Qantara,North Sinai,Egypt over two consecutive seasons(2019-2020 and 2020-2021),olive Kalamata trees were evaluated under three organic fertilizer treatments alone or in combination with three bio-fertilizers treatments.Organic fertilizer was applied as goat manure(16.8 kg/tree/year),or olive pomace(8.5 kg/tree/year)in mid-December of each season vs.untreated trees.The bio-fertilizers were applied as N-fixing bacteria(150 g/tree)was inculated in early March of each season,or amino acid mixture(1.5%)was applied three times,at 70%of full bloom,21 days after full bloom,and a month later in comparison to a non-fertilized trees(control).The cultivar used was Kalamata,a dual-purpose cultivar for oil and table olives whose value increases when processed as table olives.The results indicated that the goat manure followed by olive pomace significantly enhanced photosynthetic pigments(chlorophyll a,b,and carotenoids),leaf mineral contents(N,P,K,Ca,Mg and Fe),tree canopy volume,number of flowers per inflorescence,number of inflorescences per shoot,initial fruit set,fruit retention.For fruit quality,fruit length and width,fruit weight,and total fruit yield was increased compared to the non-fertilized control.Likewise,The bio-fertilizer N-fixing bacteria followed by the amino acid mixture significantly improved all of the aforementioned parameters.Accordingly,it is recommended,both environmentally and economically to utilize organic and bio-fertizers,particularly goat manure combined with N-fixing bacteria,in low-fertility soil to sustain olive production as well as reducing mineral fertilization. 展开更多
关键词 Organic and bio-fertilizers olivES kalamata vegetative growth leaf mineral contents fruit quality
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Production of Biogas from Olive Mill Waste Waters Treated by Cow Manure
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作者 Fatma Laabidi Rafik Besbès Bassem Jamoussi 《Green and Sustainable Chemistry》 CAS 2023年第1期62-71,共10页
The olive mill waste waters (OMWW) generated from olive oil extraction is a major environmental concern since they are characterized by their role as a pollutant (high organic and mineral matters) and their pH acid. T... The olive mill waste waters (OMWW) generated from olive oil extraction is a major environmental concern since they are characterized by their role as a pollutant (high organic and mineral matters) and their pH acid. The aim of this study was to valorize (OMWW) by anaerobic fermentation in the presence of cow manure in order to produce biogas and reduce their toxic load. Many tests were carried out by fermenting (OMWW) with polyphenols in the presence of cow manure in thermophile temperatures. The performance of this treatment was valuated through measurements of biogas production and by the determination of different parameters of fermented media (pH, volume of the biogas and polyphenols). 展开更多
关键词 olive Mill Waste Waters Anaerobic Fermentation Cow Manure BIOGAS POLYPHENOLS
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Olive oil ameliorate allergic response in ovalbumin-induced food allergy mouse by promoting intestinal mucosal immunity
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作者 Yu Ma Ming Liu +5 位作者 Donghui Li Jie Li Zixin Guo Yunjun Liu Shengnan Wan Yixiang Liu 《Food Science and Human Wellness》 SCIE CSCD 2023年第3期801-808,共8页
The numerous health benefits of olive oil are widely known,however,it also provides anti-allergic properties that have not yet been fully defined.In this study,the anti-allergic activity of olive oil was evaluated by ... The numerous health benefits of olive oil are widely known,however,it also provides anti-allergic properties that have not yet been fully defined.In this study,the anti-allergic activity of olive oil was evaluated by analyzing the clinical symptoms and immune-related factors in BALB/c mice that had ingested600 mg/(kg·day)olive oil for two weeks prior to the evaluation.An allergy model was subsequently constructed for analysis,the results of which showed that the olive oil reduced the scores of allergic symptoms in the mice,and up-regulated the hypothermia and the decline in the immune organ index.Moreover,fewer allergy-related cytokines and reduced intestinal inflammation was discovered in the olive oil-treated group.In addition,analysis of intestinal mucosal immune-related factors revealed that the olive oil promoted the expression of intestinal tight junction proteins(Claudin-1,Occludin,and ZO-1)and IL-22,and helped maintain the integrity of the intestinal epithelial physical barrier.Increased levels of mucin 2 andβ-defensin were also found in the intestinal mucus of the olive oil-treated mice.These findings suggest that the oral administration of olive oil effectively attenuated the ovalbumin-induced allergic immune response in the mice,and had a positive effect on intestinal epithelial mucosal immunity. 展开更多
关键词 olive oil Ovalbumin-induced food allergy Anti-food allergy Intestinal mucosal immunization
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Nesting Biology and Site Selection of Olive Ridley—A Coherence of Nature
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作者 Arun Malarvizhi P. M. Mohan 《Open Journal of Marine Science》 2023年第2期29-39,共11页
Nesting behaviour of sea turtles remains a subject to study, due to their enigmatic pattern of seasonal breeding activities. In the present study, characteristics of the breeding turtle, Olive Ridley (Lepidochely... Nesting behaviour of sea turtles remains a subject to study, due to their enigmatic pattern of seasonal breeding activities. In the present study, characteristics of the breeding turtle, Olive Ridley (Lepidochelys olivacea) at Ramnagar along N-E coast of North Andaman Islands were investigated, for the nesting periods 2016-2017. Olive Ridley is the dominant sea turtles, with more than 300 individuals nesting every season with at least a 75% hatching success rate. It is one of the conducive, highly protective and undisturbed nesting sites for Olive Ridley on North Andaman coast. In the present study an initiative is made, to identify nature’s cues and the biological characteristics of Olive Ridley, which leads it to nest at Ramnagar beach, with a high hatchling success rate. 展开更多
关键词 Sea Turtle olive Ridley Ramnagar Beach Nesting Beach Sand Grain Hatch-ling North Andaman
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Chimonanthus nitens Oliv. leaves flavonoids alleviate hyperuricemia by regulating uric acid metabolism and intestinal homeostasis in mice
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作者 Wenya Meng Lingli Chen +4 位作者 Kehui Ouyang Suyun Lin Yang Zhang Jing He Wenjun Wang 《Food Science and Human Wellness》 SCIE CSCD 2023年第6期2440-2450,共11页
This study aimed to evaluate the alleviating effect of Chimonanthus nitens Oliv.leaves flavonoids(CLF)on hyperuricemia induced by potassium oxonate in mice.The results showed that CLF lowered the serum levels of uric ... This study aimed to evaluate the alleviating effect of Chimonanthus nitens Oliv.leaves flavonoids(CLF)on hyperuricemia induced by potassium oxonate in mice.The results showed that CLF lowered the serum levels of uric acid(UA),creatinine and blood urea nitrogen,downregulated hepatic mRNA expressions of xanthine oxidase(XO),phosphate ribose pyrophosphate synthetase(PRPS)and adenosine deaminase(ADA)in hyperuricemia mice.In addition,CLF repaired renal injury by significantly down-regulating mRNA and protein expressions of renal UA reabsorption-related proteins and up-regulating the mRNA and protein expressions of UA secretory-related proteins.Finally,CLF inhibited UA synthesis and promoted UA excretion to alleviate hyperuricemia.Besides,CLF supplementation repaired the intestinal barrier function as demonstrated by significant increased mRNA levels of intestinal zonula occludens-1(ZO-1),Occludin,mucin 2(MUC2)and mucin 4(MUC4),as well as decreased mRNA levels of toll-like receptor 4(TLR4)and myeloid differentiation factor 88(MyD88)in mice.Further research showed that CLF treatment restored intestinal homeostasis mediated by improving the composition of gut microbiota and elevating the abundance of beneficial bacteria like Lactobacillus,Alistipes,Prevotellaceae_UCG-001 and Parasutterella.Overall,our findings revealed a novel function of CLF as a promising therapeutic candidate for the treatment of hyperuricemia. 展开更多
关键词 Chimonanthus nitens oliv.leaves FLAVONOIDS HYPERURICEMIA Renal damage Gut microbiota
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The Probable Cause for Nesting Pattern of Olive Ridley (Lepidochelys olivacea) at Ramnagar Beach, North East Coast of Andaman Island, India
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作者 Arun Malarvizhi M. M. Ilaamurughu P. M. Mohan 《Open Journal of Marine Science》 2023年第1期7-27,共21页
The nesting behaviour of sea turtles remains a subject to study, due to their enigmatic pattern of seasonal breeding activities. Over a period of time, several reports have been made in this context associated with th... The nesting behaviour of sea turtles remains a subject to study, due to their enigmatic pattern of seasonal breeding activities. Over a period of time, several reports have been made in this context associated with the nesting behaviour of the Olive Ridley turtles. In the present study, characteristics of the breeding beach and nesting pattern of Olive Ridley (Lepidochelys olivacea) at Ramnagar along N-E coast of Andaman Islands were investigated, during the nesting periods 2016-2017. The study area hosts Olive Ridley, the dominant sea turtles with more than 300 individuals nesting each year. For this study, the number of sea turtles visited, nested, the sediment characters, salinity, and temperature were taken. The exposed sandy nesting beach characteristics are prone to varying degrees of morphological changes every day. The results depict that even though similar grain size (Coarse Sand to Fine Sand and Very well sorted to Poorly Sorted), with an ambient incubating temperature, pH and salinity with wide nesting area, the selective nesting in the particular location of the beach identified because of comfortable energy conditions in the waters (1.5 m/s) favours the female turtles to reach the beach at the preferable site of Ramnagar and nest. 展开更多
关键词 Sea turtle olive Ridley Ramnagar Beach Nesting Environment Morphological Changes Sand Grain HATCHLING North Andaman
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橄榄仁抗氧化肽的分离鉴定及分子对接 被引量:2
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作者 张迎阳 董亚云 +1 位作者 邹平 郑文武 《食品与发酵工业》 CAS CSCD 北大核心 2024年第5期189-198,共10页
该研究利用酶解法结合凝胶色谱柱从橄榄仁中分离纯化得到6个抗氧化肽,采用分子对接模拟6种抗氧化肽与超氧化物歧化酶(superoxide dismutase, SOD)的结合机制。采用酶解法从橄榄仁中提取抗氧化活性肽,以水解度、DPPH自由基清除率为指标,... 该研究利用酶解法结合凝胶色谱柱从橄榄仁中分离纯化得到6个抗氧化肽,采用分子对接模拟6种抗氧化肽与超氧化物歧化酶(superoxide dismutase, SOD)的结合机制。采用酶解法从橄榄仁中提取抗氧化活性肽,以水解度、DPPH自由基清除率为指标,从6种商用酶中筛选出最适蛋白酶为木瓜蛋白酶,以底物浓度、酶底比、酶解时间、温度进行单因素试验,选取影响酶解反应的3个因素(酶底比>温度>时间)进行响应面法优化得出最佳工艺条件为底物质量浓度50 g/L、酶底比为3.3%(质量分数)、温度20℃、时间88 min。对最优条件下的粗肽液进行SephadexG-25和SephadexG-15分离纯化得到6个具有良好活性的抗氧化肽,分别为WLSF、TSVLR、LVYLVR、AGGKPFQ、YYPFKGFA、AFNVDERLAR。TSVLR显示出最高的DPPH自由基清除活性81.07%和羟自由基清除能力42.48%。分子对接研究表明,纯化的肽通过疏水效应和氢键与抗氧化相关的关键蛋白SOD有效结合。结果表明,橄榄仁可作为天然食物来源制取功能性食品的一个新的探究方向。 展开更多
关键词 橄榄仁 抗氧化肽 响应面优化 分离鉴定 分子对接
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富含玉米黄质色素橄榄油延缓D-半乳糖诱导的小鼠衰老作用
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作者 叶文斌 陈文东 +3 位作者 王昱 何九军 黄新异 邸多隆 《现代食品科技》 CAS 北大核心 2024年第6期66-73,共8页
该文研究了富含玉米黄质色素橄榄油(Olive Oil Rich Zeaxanthin,OZT)对衰老小鼠的影响。将亚急性衰老模型造模成功的小鼠分为模型组、OZT高中低处理组、橄榄油组(Olive Oil,OL)、维生素E阳性(Vitamin E,VE)对照组,以生理盐水为CK(0)组... 该文研究了富含玉米黄质色素橄榄油(Olive Oil Rich Zeaxanthin,OZT)对衰老小鼠的影响。将亚急性衰老模型造模成功的小鼠分为模型组、OZT高中低处理组、橄榄油组(Olive Oil,OL)、维生素E阳性(Vitamin E,VE)对照组,以生理盐水为CK(0)组。连续灌胃28 d后,分析不同剂量OZT对衰老小鼠的体重、脾脏和胸腺指数、耐缺氧和抗疲劳作用、血清中SOD(Superoxide Dismutase)、CAT(Catalase)、GSH-Px(Glutathione Peroxidase)、ALT(Alanine Aminotransferase)、AST(Aspartate Aminotransferase)活性和MDA(Malondialdehyde)、IL-2(Interleukin-2)、IL-4、IL-10、IFN-γ(Interferon-γ)生成水平等指标的影响。结果显示,建模灌喂给药28 d后,Model组体质量与CK组小鼠相比存在极显著差异(P<0.01),而灌胃OZT的高中低剂量小鼠的体质量之间均存在显著差异(P<0.05);灌胃后OL组小鼠的耐缺氧时间和游泳时间最短,而OZT的高剂量组的小鼠时间最长,与VE组相比无显著差异(P>0.05);OZT还能降低衰老小鼠血清中的ALT、AST、SOD、CAT和GSH-Px的活性,提高IL-2、IL-4、IL-10和IFN-γ生成,降低MDA含量,且OZT高剂量组效果显著优于OL组,与VE接近。综上,OZT对D-半乳糖致亚急性衰老动物模型有很好的干预效果,能有效提高小鼠机体抗氧化能力,延长耐缺氧时间和提高抗疲劳作用,延缓衰老的作用。 展开更多
关键词 玉米黄质色素 橄榄油 衰老小鼠 影响
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基于特级初榨橄榄油两种不同特性的DSC掺假分析方法研究 被引量:1
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作者 魏燕 宋志强 武卫东 《食品与发酵工业》 CSCD 北大核心 2024年第1期183-188,共6页
该文基于油脂物理特性与氧化稳定性2种特性,利用差示扫描量热法(differential scanning calorimetry,DSC)对市面上常见的特级初榨橄榄油(extra virgin olive oil,EVOO)中大豆油掺假问题进行研究。在物理特性方面,采用DSC冷却曲线来分析E... 该文基于油脂物理特性与氧化稳定性2种特性,利用差示扫描量热法(differential scanning calorimetry,DSC)对市面上常见的特级初榨橄榄油(extra virgin olive oil,EVOO)中大豆油掺假问题进行研究。在物理特性方面,采用DSC冷却曲线来分析EVOO掺假。随着大豆油掺假比例升高,油样在-40~-60℃结晶焓值ΔH由EVOO的29.78 J/g减小为40%掺假混合油的6.133 J/g,对EVOO质量比构建的一元回归方程Y=57.556X-28.44(R2=0.997)。在氧化稳定性方面,氧化温度160℃下,不同油样诱导氧化时间随大豆油掺假比例上升逐渐变短,由EVOO的25.8 min逐渐减小为40%掺假混合油的17.8 min,Y=-19.4X+25.64(R^(2)=0.996),掺假模型相关性较好。这2种DSC检验掺假分析方法都能有效鉴别EVOO中大豆油的掺假且各有特点,可根据实际情况选取合适的方法更好处理EVOO掺假问题。 展开更多
关键词 特级初榨橄榄油 大豆油 物理特性 氧化稳定性 差示扫描量热法 掺假鉴定
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基于代谢组学和网络药理学的橄榄类黄酮组分评价
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作者 赖瑞联 沈朝贵 +3 位作者 龙宇 林炎娟 韦晓霞 吴如健 《食品科学》 EI CAS CSCD 北大核心 2024年第12期42-51,共10页
目的:探究橄榄类黄酮组分的潜在功效及其在不同种质资源中的差异。方法:通过网络药理学和分子对接技术挖掘橄榄功效类黄酮组分,并结合代谢组学评价橄榄种质资源中类黄酮组分的积累水平。结果:从橄榄中鉴定获得44种类黄酮组分,基于TCMSP... 目的:探究橄榄类黄酮组分的潜在功效及其在不同种质资源中的差异。方法:通过网络药理学和分子对接技术挖掘橄榄功效类黄酮组分,并结合代谢组学评价橄榄种质资源中类黄酮组分的积累水平。结果:从橄榄中鉴定获得44种类黄酮组分,基于TCMSP数据库、类药性和口服生物利用度筛选获得18种潜在的功效类黄酮组分及对应的180个作用靶点,其中木犀草素、槲皮素、儿茶素和表儿茶素是潜在影响橄榄药理活性的重要化学物质。基于类黄酮组分与靶点连通度筛选的35个关键作用靶点共富集到348条基因本体通路和136条京都基因与基因组百科全书信号通路,涉及抗癌、抗病毒、抗菌消炎、调节血糖和保护肝脏等药理活性。通过分子对接及可视化分析表明,所筛选的橄榄功效类黄酮组分能与关键作用靶点稳定结合。结论:代谢组学和网络药理学可为橄榄药用价值开发提供依据,橄榄类黄酮组分通过多成分-多靶点-多通路协同作用发挥多样化的药理作用。 展开更多
关键词 橄榄 种质资源 代谢组学 网络药理学 类黄酮
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超声波辅助橄榄油提取大麻二酚工艺研究
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作者 高宝昌 石雨 +7 位作者 田媛 李国巍 李纯思 张正海 王义志 张治国 徐海鑫 孙宇峰 《黑龙江科学》 2024年第8期6-9,13,共5页
以工业大麻花叶为原料,采用超声波辅助橄榄油提取大麻二酚(CBD),利用中心组合(CCD)方法对提取工艺的4种因素进行优化。实验结果表明,最佳提取条件为:超声波功率95 W、提取温度62℃、提取时间45 min、料液比1:9(g/mL),该条件下CBD浓度为3... 以工业大麻花叶为原料,采用超声波辅助橄榄油提取大麻二酚(CBD),利用中心组合(CCD)方法对提取工艺的4种因素进行优化。实验结果表明,最佳提取条件为:超声波功率95 W、提取温度62℃、提取时间45 min、料液比1:9(g/mL),该条件下CBD浓度为39.01μg/mL,提取率达到91.35%,此结果为CBD的多元化开发应用奠定了技术基础。 展开更多
关键词 工业大麻 大麻二酚 橄榄油 响应面法
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不同种植年限川党参根际土壤细菌群落结构研究
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作者 罗敏 朱晓富 +5 位作者 徐广 罗舜 卢胜鄂 郭瑀 杨丹 邓才富 《中国土壤与肥料》 CAS CSCD 北大核心 2024年第3期32-39,共8页
为了探究生长年限对川党参根际土壤细菌群落结构特征及其多样性的影响,阐明细菌群落变化规律,采用Illumina MiSeq高通量测序技术对1年生、2年生、3年生和4年生的川党参根际土壤细菌16S rRNA序列进行测序分析。结果表明:(1)生长年限显著... 为了探究生长年限对川党参根际土壤细菌群落结构特征及其多样性的影响,阐明细菌群落变化规律,采用Illumina MiSeq高通量测序技术对1年生、2年生、3年生和4年生的川党参根际土壤细菌16S rRNA序列进行测序分析。结果表明:(1)生长年限显著影响川党参根际土壤细菌群落多样性,其OTU数、Alpha多样性指数均随生长年限增加呈先升高后降低的趋势;(2)生长年限改变了细菌群落组成,但在门水平上优势菌门(相对丰度>5%)相对稳定,均为变形菌门(Proteobacteria)、未分类细菌门(unclassified_Bacteria)、酸杆菌门(Aci-dobacteria)、放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes)和绿弯菌门(Chloroflexi),其相对丰度总和占85.71%~91.39%;在属水平上优势菌群相差较大,仅有全噬菌属(Holophaga)和念珠菌属(Candidatus Udaeo-bacter)是4个年限所共有的优势菌属,革兰氏阴性菌cTPY-13(Gram-negative bacterium cTPY-13)则为2年生独有优势菌属,其余则分别为不同年限有相应共有优势菌属;(3)PCoA分析与UPGMA聚类分析显示,各生长年限间细菌群落差异明显,随生长年限增加,不同组间细菌群落结构差异先增大后逐渐减小,细菌群落在2~3年生时演变较为剧烈;(4)土壤速效养分是影响根际土壤细菌群落的主要环境因子,其程度大小为水解氮(HN)>有效磷(AP)>速效钾(AK),优势菌门中酸杆菌门与AP、AK呈显著负相关(P<0.05),拟杆菌门与AP、AK呈显著正相关(P<0.05),而绿弯菌门与HN呈显著负相关(P<0.05)。综上所述,川党参生长年限延长会影响根际土壤细菌群落结构和多样性,从而改变其根际微生态系统。 展开更多
关键词 川党参 根际土壤 细菌群落 高通量测序
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杜仲协同拟茎点霉XP-8合成木脂素类的条件优化及其功能活性
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作者 朱静 陈晖 +1 位作者 陈亚蓝 师俊玲 《食品工业科技》 CAS 北大核心 2024年第15期360-368,共9页
对杜仲协同拟茎点霉XP-8生物合成松脂醇(Pinoresinol,Pin)、松脂醇单葡萄糖苷(Pinoresinol-4-O-β-Dglucopyranoside,PMG)和松脂醇二葡萄糖苷(Pinoresinol diglucoside,PDG)的条件进行优化,研究培养方式、杜仲添加量和静息处理时间对Pin... 对杜仲协同拟茎点霉XP-8生物合成松脂醇(Pinoresinol,Pin)、松脂醇单葡萄糖苷(Pinoresinol-4-O-β-Dglucopyranoside,PMG)和松脂醇二葡萄糖苷(Pinoresinol diglucoside,PDG)的条件进行优化,研究培养方式、杜仲添加量和静息处理时间对Pin、PMG和PDG产量的影响,并对相关产物的抗氧化性和抑癌性进行研究。结果表明,3%杜仲PDB培养基培养菌体并静息处理5 d时Pin、PMG和PDG产量较高。该条件下代谢产物液对DPPH和ABTS+自由基的清除能力显著高于杜仲空白培养基,说明发酵过程中产生了增强抗氧化功能的物质。其还能抑制肝癌细胞HepG-2的增殖,通过纯品验证活性说明,Pin是其中主要抑制癌细胞的活性成分,代谢产物液抑制肝癌细胞的作用是一种协同效应。 展开更多
关键词 木脂素类物质 拟茎点霉XP-8 杜仲 抗氧化 抑癌
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响应面结合模糊数学感官评价法优化油橄榄果渣曲奇饼干的配方及其品质测定
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作者 樊亮 叶文斌 +3 位作者 胡文斌 杨文 孙娜 王林林 《甘肃农业大学学报》 CAS CSCD 北大核心 2024年第2期282-295,共14页
【目的】开发油橄榄果渣曲奇饼干。【方法】以低筋面粉、黄油和鲜鸡蛋为材料复配陇南油橄榄果渣、麦芽糖醇、金耳和纹党多糖,添加碳酸氢钠和氯化钠等食品添加剂研制油橄榄果渣低糖复合曲奇饼干,采用响应面结合模糊数学感官评价法优化了... 【目的】开发油橄榄果渣曲奇饼干。【方法】以低筋面粉、黄油和鲜鸡蛋为材料复配陇南油橄榄果渣、麦芽糖醇、金耳和纹党多糖,添加碳酸氢钠和氯化钠等食品添加剂研制油橄榄果渣低糖复合曲奇饼干,采用响应面结合模糊数学感官评价法优化了配方,对其抗氧化性、理化指标和微生物指标进行了检验。【结果】油橄榄果渣添加量为15%、麦芽糖醇添加量为30%、金耳添加量为10%和纹党多糖添加量为0.35%,曲奇饼干形态完整,断面结构细密、无粉末,硬度适中,薄厚均匀、口感细腻、咀嚼性好,酥脆、无颗粒感,油橄榄风味突出,气味协调、颜色均一卫生指标符合国家标准,具有很好的营养价值和抗氧化功能。【结论】为油橄榄果渣的进一步利用提供了参考。 展开更多
关键词 油橄榄果渣 曲奇饼干 配方优化 响应面试验 模糊数学法 抗氧化
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种苗期川党参根际土壤细菌群落变化研究
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作者 罗敏 邓才富 +2 位作者 朱晓富 徐广 罗舜 《亚太传统医药》 2024年第1期51-55,共5页
目的:研究川党参种苗期根际土壤细菌群落的结构特征和多样性变化,掌握种苗期土壤微生物群落的演替规律,为川党参连作障碍的防治提供理论依据。方法:采集1年生(Op1)和2年生(Op2)的川党参根际土壤,采用Illumina MiSeq高通量测序技术进行细... 目的:研究川党参种苗期根际土壤细菌群落的结构特征和多样性变化,掌握种苗期土壤微生物群落的演替规律,为川党参连作障碍的防治提供理论依据。方法:采集1年生(Op1)和2年生(Op2)的川党参根际土壤,采用Illumina MiSeq高通量测序技术进行细菌16S rRNA序列V3-V4区的高通量测序,对种苗期根际土壤细菌群落变化进行分析。结果:通过MiSeq测序共得到882369条优化序列,其中1年生注释到9926个OTU,2年生注释到7560个OTU。种苗期随生长年限的增加,川党参根际土壤速效养分、细菌群落丰富度和多样性均呈增加趋势。在细菌门分类水平上,1年生有5个优势菌群(相对丰度均大于5%),2年生有7个优势菌群,共有优势菌群为变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes);在属分类水平上排名前五菌群中只有全噬菌属(Holophaga)与念珠菌属(Candidatus Udaeobacter)为共有优势菌群,且1年生有11个独有菌群。PCoA分析与UPGMA聚类分析表明1年生与2年生川党参根际土壤细菌群落具有显著差异,随生长年限延长根际土壤细菌群落结构演变明显。结论:川党参种苗期根际土壤速效养分、土壤细菌群落多样性呈递增变化,细菌群落结构相似性呈递减变化。研究为揭示川党参种植和连作障碍形成的原因提供了理论依据。 展开更多
关键词 川党参 根际土壤 细菌群落 种苗期
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