The leaf development of Podocarpus macrophyllus var.maki Endl.started from the leaf primordium formed by the cell division in shoot apical meristem.During the leaf development,three resin canals from the inferior part...The leaf development of Podocarpus macrophyllus var.maki Endl.started from the leaf primordium formed by the cell division in shoot apical meristem.During the leaf development,three resin canals from the inferior part of vascular bundles firstly began to differentiate,then protoxylem in vascular bundles differentiated from adaxial side to abaxial side.Meanwhile,a layer of parenchyma cells from the inferior part of protoderm developed into the sclerenchyma,which played an important role in supporting young leaves.When xylem became mature basically,transfusion tissue,phloem and accessory transfusion tissue began to differentiate,so mesophyll tissue finally differentiated into developmental palisade cells and sponge cells.The thicker cuticle was formed in the lateral side of epidermal cells at the same time of differentiation and development for the inner structure of leaves.Therefore,the developmental palisade cells and the cuticle in the leaf structure of Podocarpus macrophyllus var.maki Endl.is greatly significant for photosynthesis and transpiration when it grows in arid and cold regions.展开更多
A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene diversity and genotype identification for clones of Sequoia sempervirens (D. Don) Endl. in Chil...A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene diversity and genotype identification for clones of Sequoia sempervirens (D. Don) Endl. in Chile. Ten (out of 34) clones from introduction trial located in Voipir-Villarrica, Chile, were studied. The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction. The PCR tests were carried out employing 10-mer random primers. The amplification products were detected by electrophoresis in agarose gels. Forty nine polymorphic bands were obtained with the selected primers (BG04, BF07, BF12, BF13, and BF14) and were ordered according to their molecular size. The genetic similarity between samples was calculated by the Jaccard index and a dendrogram was con- structed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA). Of the primers tested, 5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism. A total of 49 polymorphic RAPD bands were detected out of 252 bands. The genetic similarity analysis demonstrates an extensive genetic variability between the tested clones and the dendrogram depicts the genetic relationships among the clones, suggesting a geographic relationship. The results indicate that the RAPD markers permitted the identification of the assayed clones, although they are derived from the same geo- graphic origin.展开更多
Nutritional and functional components in leaves of Smallanthus sonchifolius （Poepp. et Endl.） H. Robinson were analyzed. The contents of crude protein and total amino acid were 14.533% and 13.18% respectively. It wa...Nutritional and functional components in leaves of Smallanthus sonchifolius （Poepp. et Endl.） H. Robinson were analyzed. The contents of crude protein and total amino acid were 14.533% and 13.18% respectively. It was rich in contents of polysaccharide （22.793%）, flavone (0.089 8 mg·g-1) and mineral elements. This species can be utilized widely in functional food.展开更多
The essential oil was extracted from fresh leaf of Chamaecyparis pisifera(Sieb.et Zucc.) Endl.using steam distillation.Chemical components in the essential oil were identified by GC-MS and their relative contents were...The essential oil was extracted from fresh leaf of Chamaecyparis pisifera(Sieb.et Zucc.) Endl.using steam distillation.Chemical components in the essential oil were identified by GC-MS and their relative contents were determined by peak area normalization.The results show that the extraction rate of the essential oil is 0.23%,and fifty-four compounds in the essential oil are identified.The main chemical components are cedrol,3,5-dimethyl-4-benzyl-isoxazole,(5α)-D-homoandrostan-17a-one,etc,and their relative contents are 32.32%,11.31% and 5.64%,respectively.展开更多
基金Supported by Natural Basic Research Program of Universities in Jiangsu(06KJD180201)~~
文摘The leaf development of Podocarpus macrophyllus var.maki Endl.started from the leaf primordium formed by the cell division in shoot apical meristem.During the leaf development,three resin canals from the inferior part of vascular bundles firstly began to differentiate,then protoxylem in vascular bundles differentiated from adaxial side to abaxial side.Meanwhile,a layer of parenchyma cells from the inferior part of protoderm developed into the sclerenchyma,which played an important role in supporting young leaves.When xylem became mature basically,transfusion tissue,phloem and accessory transfusion tissue began to differentiate,so mesophyll tissue finally differentiated into developmental palisade cells and sponge cells.The thicker cuticle was formed in the lateral side of epidermal cells at the same time of differentiation and development for the inner structure of leaves.Therefore,the developmental palisade cells and the cuticle in the leaf structure of Podocarpus macrophyllus var.maki Endl.is greatly significant for photosynthesis and transpiration when it grows in arid and cold regions.
基金Project (No.CONICYT-FONDEF PROYECT D01 I 1008) supported by the National Commission of Science and Research of Chile
文摘A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene diversity and genotype identification for clones of Sequoia sempervirens (D. Don) Endl. in Chile. Ten (out of 34) clones from introduction trial located in Voipir-Villarrica, Chile, were studied. The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction. The PCR tests were carried out employing 10-mer random primers. The amplification products were detected by electrophoresis in agarose gels. Forty nine polymorphic bands were obtained with the selected primers (BG04, BF07, BF12, BF13, and BF14) and were ordered according to their molecular size. The genetic similarity between samples was calculated by the Jaccard index and a dendrogram was con- structed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA). Of the primers tested, 5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism. A total of 49 polymorphic RAPD bands were detected out of 252 bands. The genetic similarity analysis demonstrates an extensive genetic variability between the tested clones and the dendrogram depicts the genetic relationships among the clones, suggesting a geographic relationship. The results indicate that the RAPD markers permitted the identification of the assayed clones, although they are derived from the same geo- graphic origin.
文摘Nutritional and functional components in leaves of Smallanthus sonchifolius （Poepp. et Endl.） H. Robinson were analyzed. The contents of crude protein and total amino acid were 14.533% and 13.18% respectively. It was rich in contents of polysaccharide （22.793%）, flavone (0.089 8 mg·g-1) and mineral elements. This species can be utilized widely in functional food.
文摘The essential oil was extracted from fresh leaf of Chamaecyparis pisifera(Sieb.et Zucc.) Endl.using steam distillation.Chemical components in the essential oil were identified by GC-MS and their relative contents were determined by peak area normalization.The results show that the extraction rate of the essential oil is 0.23%,and fifty-four compounds in the essential oil are identified.The main chemical components are cedrol,3,5-dimethyl-4-benzyl-isoxazole,(5α)-D-homoandrostan-17a-one,etc,and their relative contents are 32.32%,11.31% and 5.64%,respectively.
文摘在拉萨当雄拉屋矿山尾矿库设置客土+鸡蛋框、客土+保水剂、客土+保水剂+羊粪、客土+鸡蛋框+羊粪、客土+鸡蛋框+保水剂、客土+鸡蛋框+保水剂+羊粪和对照7种处理进行植被恢复,研究了不同恢复措施对爬地柏(Sabina procumbens(Endl.)Iwata et Kusaka)生长指标和生理生化指标的影响。结果表明:爬地柏在不同处理下,苗高、地径和冠幅都有了不同程度的提高,其中提高最多的是客土+鸡蛋框+保水剂+羊粪处理,苗高、地径、平均冠幅分别比对照提高了100.99%、49.23%、102.31%;生理指标方面,客土+鸡蛋框+保水剂+羊粪处理叶片相对含水量最高,为82.11%,比对照提高了54.37%,叶绿素a和叶绿素b质量分数最多,可溶性糖、脯氨酸质量分数及丙二醛摩尔质量浓度最低,且所有指标与其它处理都差异显著,说明"客土+鸡蛋框+保水剂+羊粪"处理较适于尾矿治理。
