[Objective] The study was to immunohistochemical localize the endocrine cells from digestive tract of Misgurnus [ Method ] Using six gastrointestinal hormone antisera, the endocrine cells from digestive tract of M. an...[Objective] The study was to immunohistochemical localize the endocrine cells from digestive tract of Misgurnus [ Method ] Using six gastrointestinal hormone antisera, the endocrine cells from digestive tract of M. anguillicaudatus was localized. [ Result ]The 5-hydroxytryptamine immunoreactive（5-HT-IR） cells distribute in oesophagus, foregut and midgut; the distribution density was determined to be forepart of foregut 〉 oesophagus and hindpart of foregut 〉 gut, and the differences in the three density gradients reached significant level. Like PP-IR, SS-IR cells were observed mostly in oesophagus, followed by hindpart of foregut, least in forepart of foregut, but never found in gut and hindgut. The three kinds of immunocompetent cells Gas-IR, Glu-IR and SP-IR were not detected in each part of digestive tract. [ Conctusion] This study may provide basic data for studying the nutritional and digestive physiology, as well as the preparation of meridic diets for M. anguillicaudatus.展开更多
AIM： To darify the types, regional distributions and distribution densities as well as morphological features of gastrointestinal （GI） endocrine cells in various parts of the gastrointestinal track （GIT） of four ...AIM： To darify the types, regional distributions and distribution densities as well as morphological features of gastrointestinal （GI） endocrine cells in various parts of the gastrointestinal track （GIT） of four reptiles, Gekko japonicus, Eumeces chinensis, Sphenomorphus indicus and Eumeces elegans. METHODS： Paraffin-embedded sections （5μm） of seven parts （cardia, fundus, pylorus, duodenum, jejunum, ileum, rectum） of GIT dissected from the four reptiles were prepared. GI endocrine cells were revealed by using immunohistochemical techniques of streptavidin-peroxidase （S-P） method. Seven types of antisera against 5-hydroxytryptamine （5-HT）, somatostatin （SS）, gastrin （GAS）, glucagon （GLU）, substance P （SP）, insulin and pancreatic polypeptide were identified and then GI endocrine cells were photomicrographed and counted. RESULTS： The GI endocrine system of four reptiles was a complex structure containing many endocrine cell types similar in morphology to those found in higher vertebrates. Five types of GI endocrine cells, namely 5-HT, SS, GAS, SP and GLU immunoreactive （IR） cells were identified in the GIT of G. faponicus, E. chinensis and S. indicus, while in the GIT of E. elegans only the former three types of endocrine cells were observed. No PP- and INS- IR cells were found in all four reptiles. 5-HT-IR cells, which were most commonly found in the pylorus or duodenum, distributed throughout the whole GIT of four reptiles. However, their distribution patterns varied from each other. SS-IR ceils, which were mainly found in the stomach especially in the pylorus and/or fundus, were demonstrated in the whole GIT of E. chinensis, only showed restricted distribution inthe other three species. GAS-IR cells, with a much restricted distribution, were mainly demonstrated in the pylorus and/or the proximal small intestine of four reptiles. GLU-IR cells exhibited a limited and species-dependent variant distribution in the GIT of four reptiles. SP-IR cells were found throughout the GIT except for jejunum in E. elegans and showed a restricted distribution in the GIT of G. japonicus and S. indicus. In the GIT of four reptiles the region with the highest degree of cell type heterogeneity was pylorus and most types of GI endocrine cells along the GIT showed the peak density in pylorus as well. CONCLUSION： Some common and unique features of the distribution and morphology of different types of GI endocrine cells are found in four reptiles. This common trait may reflect the similarity in digestive physiology of various vertebrates.展开更多
目的研究中华蟾蜍(Bufo gar garizans)变态发育过程中消化道5-羟色胺(5-HT)、生长抑素(SS)、胃泌素(GAS)、胰高血糖素(GLU)和胰多肽(PP)等5种胃肠激素内分泌细胞的分布密度、组织学定位及形态的演变。方法应用链霉卵白素-过氧化物酶(SP...目的研究中华蟾蜍(Bufo gar garizans)变态发育过程中消化道5-羟色胺(5-HT)、生长抑素(SS)、胃泌素(GAS)、胰高血糖素(GLU)和胰多肽(PP)等5种胃肠激素内分泌细胞的分布密度、组织学定位及形态的演变。方法应用链霉卵白素-过氧化物酶(SP)免疫组织化学方法,每个变态发育时期取6只动物。结果 5-HT细胞在中华蟾蜍变态发育各个时期消化道中均有分布,在孵出期起始出现于消化道中部,变态前期之后整个消化道均有分布,消化道中部5-HT细胞的密度显著增加(P<0.05),从变态高峰期至幼蟾期,食道和贲门5-HT细胞的密度增加非常显著(P<0.05);GAS细胞在幼蟾期才开始出现,主要集中于十二指肠,幽门处偶见;SS细胞在变态高峰期开始出现,仅在胃体和十二指肠偶有分布,幼蟾期分布于食道至空肠的各段,细胞密度显著增加(P<0.05);GLU细胞在中华蟾蜍变态发育各个时期消化道中均有分布,变态前期之后消化道中部GLU细胞的分布密度增加非常显著(P<0.05);PP细胞在变态发育的各个时期均未检测出。在孵出期,内分泌细胞主要位于固有层,呈圆形;变态早期和前期,主要位于单层柱状上皮细胞之间,呈圆形、椭圆形或梭形;变态高峰期,内分泌细胞主要位于腺泡细胞或上皮细胞基部,固有膜中偶见,呈椭圆形或锥形;在幼蟾期,主要位于腺泡细胞和黏膜上皮细胞之间,呈圆形或椭圆形,且个别细胞具有较长的胞突伸向肠腔。结论与其他两栖动物相比,中华蟾蜍消化道内分泌细胞在变态发育过程中的演变既有一定的共性,也存在着种间差异,内分泌细胞的演变与消化生理活动及个体生理活动的变化相适应。展开更多
We studied the distribution and density of the endocrine cells in the digestive tract of Paa spinosa using immunohistochemical method (streptavidin peroxidase method) using eight gut hormone antisera. The 5 hydroxytry...We studied the distribution and density of the endocrine cells in the digestive tract of Paa spinosa using immunohistochemical method (streptavidin peroxidase method) using eight gut hormone antisera. The 5 hydroxytryptamine immunoreactive cells were distributed throughout the digestive tract with the highest density in the stomachus pyloricus, the second highest in the duodenum, fewer in the oesophagus, stomachus cardiacus and rectum. The gastrin immunoreactive cells were located mainly in the stomachus pyloricus and occasionally in different parts of the intestine. The somatostatin immunoreactive cells occured mainly in the stomach, frequently in the stomachus pyloricus, and occasionally in different parts of the intestine. The pancreatic polypeptide immunoreaetive cells were found with the highest density in the duodenum, the second highest in the stomachus cardiacus, and rarely in the rectum. No immunoreactive cells were observed with the antisera to glucagon, substance P, growth hormone and calcitonin. but there were glucagon and substance P mucosal nerve plexus throughout the digestive tract, and both with the highest density in the duodenum .展开更多
Immunohistochemical techniques incorporating the streptavidin peroxidase method were used to study the morphological structure and the distribution density of 5-hydroxytryptamine(5-HT),Somatostatin(SS),Gastrin(Gas),Gl...Immunohistochemical techniques incorporating the streptavidin peroxidase method were used to study the morphological structure and the distribution density of 5-hydroxytryptamine(5-HT),Somatostatin(SS),Gastrin(Gas),Glucagon(Glu),Pancreatic Polypeptide(PP) and Substance P(SP) in the digestive tract of the Arctic fox(Alopex lagopus).The results indicated that the shape of endocrine cells in the digestive tract were of many forms: round,ellipse or spindle shape.The endocrine cells were mainly located between the epithelia of the gastric gland,intestine and intestinal gland.The distributive density of 5-HT immunoreactive cells were the maximum in the colon,medium in the rectum and jejunum,and the least in the fundus,duodenum,ileum,and pylorus.They were not found in the esophagus,cardia or cecum.SS positive cells were located in the pylorus mostly,but were not detected in the esophagus,cardia and cecum.Gas positive cells were the most in the duodenum,while they were not found in the esophagus,cardia,colon,rectum and cecum.Glu positive cells were distributed in the pylorus,fundus and jejunum,but were not found in other parts of digestive tract.PP positive cells were the most in the jejunum,the second-most in frequency in the colon and rectum,while they were not found in the other parts.SP positive cells were mainly distributed in the pylorus,while fewer were found in the colon,and they were not found in other parts.The location of 5-HT positive cells were mostly distributed in the colon.SS,Gas,Glu and SP positive cells were mostly distributed in the pylorus,and PP positive cells were mostly distributed in the jejunum.展开更多
基金Supported by Anhui Key Laboratory of Plant Resources and Biology~~
文摘[Objective] The study was to immunohistochemical localize the endocrine cells from digestive tract of Misgurnus [ Method ] Using six gastrointestinal hormone antisera, the endocrine cells from digestive tract of M. anguillicaudatus was localized. [ Result ]The 5-hydroxytryptamine immunoreactive（5-HT-IR） cells distribute in oesophagus, foregut and midgut; the distribution density was determined to be forepart of foregut 〉 oesophagus and hindpart of foregut 〉 gut, and the differences in the three density gradients reached significant level. Like PP-IR, SS-IR cells were observed mostly in oesophagus, followed by hindpart of foregut, least in forepart of foregut, but never found in gut and hindgut. The three kinds of immunocompetent cells Gas-IR, Glu-IR and SP-IR were not detected in each part of digestive tract. [ Conctusion] This study may provide basic data for studying the nutritional and digestive physiology, as well as the preparation of meridic diets for M. anguillicaudatus.
基金Supported by Special Foundation for Anhui Young Leaders Fellowship, No. 04043049, Natural Science Foundation of Anhui Province, No. 01043501, Special Foundation on Key Laboratories on Conservation and Use of Important Biological Resources of Anhui Province and Nature Science Research Program for Young Teacher in University of Anhui Province, No. 2004jq0123
文摘AIM： To darify the types, regional distributions and distribution densities as well as morphological features of gastrointestinal （GI） endocrine cells in various parts of the gastrointestinal track （GIT） of four reptiles, Gekko japonicus, Eumeces chinensis, Sphenomorphus indicus and Eumeces elegans. METHODS： Paraffin-embedded sections （5μm） of seven parts （cardia, fundus, pylorus, duodenum, jejunum, ileum, rectum） of GIT dissected from the four reptiles were prepared. GI endocrine cells were revealed by using immunohistochemical techniques of streptavidin-peroxidase （S-P） method. Seven types of antisera against 5-hydroxytryptamine （5-HT）, somatostatin （SS）, gastrin （GAS）, glucagon （GLU）, substance P （SP）, insulin and pancreatic polypeptide were identified and then GI endocrine cells were photomicrographed and counted. RESULTS： The GI endocrine system of four reptiles was a complex structure containing many endocrine cell types similar in morphology to those found in higher vertebrates. Five types of GI endocrine cells, namely 5-HT, SS, GAS, SP and GLU immunoreactive （IR） cells were identified in the GIT of G. faponicus, E. chinensis and S. indicus, while in the GIT of E. elegans only the former three types of endocrine cells were observed. No PP- and INS- IR cells were found in all four reptiles. 5-HT-IR cells, which were most commonly found in the pylorus or duodenum, distributed throughout the whole GIT of four reptiles. However, their distribution patterns varied from each other. SS-IR ceils, which were mainly found in the stomach especially in the pylorus and/or fundus, were demonstrated in the whole GIT of E. chinensis, only showed restricted distribution inthe other three species. GAS-IR cells, with a much restricted distribution, were mainly demonstrated in the pylorus and/or the proximal small intestine of four reptiles. GLU-IR cells exhibited a limited and species-dependent variant distribution in the GIT of four reptiles. SP-IR cells were found throughout the GIT except for jejunum in E. elegans and showed a restricted distribution in the GIT of G. japonicus and S. indicus. In the GIT of four reptiles the region with the highest degree of cell type heterogeneity was pylorus and most types of GI endocrine cells along the GIT showed the peak density in pylorus as well. CONCLUSION： Some common and unique features of the distribution and morphology of different types of GI endocrine cells are found in four reptiles. This common trait may reflect the similarity in digestive physiology of various vertebrates.
文摘目的研究中华蟾蜍(Bufo gar garizans)变态发育过程中消化道5-羟色胺(5-HT)、生长抑素(SS)、胃泌素(GAS)、胰高血糖素(GLU)和胰多肽(PP)等5种胃肠激素内分泌细胞的分布密度、组织学定位及形态的演变。方法应用链霉卵白素-过氧化物酶(SP)免疫组织化学方法,每个变态发育时期取6只动物。结果 5-HT细胞在中华蟾蜍变态发育各个时期消化道中均有分布,在孵出期起始出现于消化道中部,变态前期之后整个消化道均有分布,消化道中部5-HT细胞的密度显著增加(P<0.05),从变态高峰期至幼蟾期,食道和贲门5-HT细胞的密度增加非常显著(P<0.05);GAS细胞在幼蟾期才开始出现,主要集中于十二指肠,幽门处偶见;SS细胞在变态高峰期开始出现,仅在胃体和十二指肠偶有分布,幼蟾期分布于食道至空肠的各段,细胞密度显著增加(P<0.05);GLU细胞在中华蟾蜍变态发育各个时期消化道中均有分布,变态前期之后消化道中部GLU细胞的分布密度增加非常显著(P<0.05);PP细胞在变态发育的各个时期均未检测出。在孵出期,内分泌细胞主要位于固有层,呈圆形;变态早期和前期,主要位于单层柱状上皮细胞之间,呈圆形、椭圆形或梭形;变态高峰期,内分泌细胞主要位于腺泡细胞或上皮细胞基部,固有膜中偶见,呈椭圆形或锥形;在幼蟾期,主要位于腺泡细胞和黏膜上皮细胞之间,呈圆形或椭圆形,且个别细胞具有较长的胞突伸向肠腔。结论与其他两栖动物相比,中华蟾蜍消化道内分泌细胞在变态发育过程中的演变既有一定的共性,也存在着种间差异,内分泌细胞的演变与消化生理活动及个体生理活动的变化相适应。
文摘We studied the distribution and density of the endocrine cells in the digestive tract of Paa spinosa using immunohistochemical method (streptavidin peroxidase method) using eight gut hormone antisera. The 5 hydroxytryptamine immunoreactive cells were distributed throughout the digestive tract with the highest density in the stomachus pyloricus, the second highest in the duodenum, fewer in the oesophagus, stomachus cardiacus and rectum. The gastrin immunoreactive cells were located mainly in the stomachus pyloricus and occasionally in different parts of the intestine. The somatostatin immunoreactive cells occured mainly in the stomach, frequently in the stomachus pyloricus, and occasionally in different parts of the intestine. The pancreatic polypeptide immunoreaetive cells were found with the highest density in the duodenum, the second highest in the stomachus cardiacus, and rarely in the rectum. No immunoreactive cells were observed with the antisera to glucagon, substance P, growth hormone and calcitonin. but there were glucagon and substance P mucosal nerve plexus throughout the digestive tract, and both with the highest density in the duodenum .
文摘Immunohistochemical techniques incorporating the streptavidin peroxidase method were used to study the morphological structure and the distribution density of 5-hydroxytryptamine(5-HT),Somatostatin(SS),Gastrin(Gas),Glucagon(Glu),Pancreatic Polypeptide(PP) and Substance P(SP) in the digestive tract of the Arctic fox(Alopex lagopus).The results indicated that the shape of endocrine cells in the digestive tract were of many forms: round,ellipse or spindle shape.The endocrine cells were mainly located between the epithelia of the gastric gland,intestine and intestinal gland.The distributive density of 5-HT immunoreactive cells were the maximum in the colon,medium in the rectum and jejunum,and the least in the fundus,duodenum,ileum,and pylorus.They were not found in the esophagus,cardia or cecum.SS positive cells were located in the pylorus mostly,but were not detected in the esophagus,cardia and cecum.Gas positive cells were the most in the duodenum,while they were not found in the esophagus,cardia,colon,rectum and cecum.Glu positive cells were distributed in the pylorus,fundus and jejunum,but were not found in other parts of digestive tract.PP positive cells were the most in the jejunum,the second-most in frequency in the colon and rectum,while they were not found in the other parts.SP positive cells were mainly distributed in the pylorus,while fewer were found in the colon,and they were not found in other parts.The location of 5-HT positive cells were mostly distributed in the colon.SS,Gas,Glu and SP positive cells were mostly distributed in the pylorus,and PP positive cells were mostly distributed in the jejunum.
