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Responses of Membrane Lipid Peroxidation and Endogenous Hormones of Soybean Seedlings to UV-B Radiation and Rare Earth 被引量:7
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作者 闫生荣 杨春和 +2 位作者 张跃群 花海蓉 蒋云霞 《Agricultural Science & Technology》 CAS 2009年第6期72-75,90,共5页
[ Objective] The aim was to provide strategies for development of rare earth and control of environmental pollution. [ Method] Responses of membrane lipid peroxidation and endogenous hormones of soybean seedlings to U... [ Objective] The aim was to provide strategies for development of rare earth and control of environmental pollution. [ Method] Responses of membrane lipid peroxidation and endogenous hormones of soybean seedlings to UV-B radiation and rare earth were studied through hydroponics in laboratory. [ Result] The results showed that under irradiation of UV-B( T1-0.15 W/m^2 and T2-0.45 W/m^2), chlorophyll and indole-3-acetic acid(IAA) contents firstly decreased during the stress phase (1 -5 d) and then increased during the restoration phase (6 -9 d) while contents of malonadialdehyde(MDA) and abscisic acid(ABA) gradually increased during the imposition of UV-B radiation (1 -5 d) and subsequently decreased during recovery from UV-B stress (6 -9 d) . With adding of La (III) with the concentration of 20 mg · L^-1 , the decline/dse trend of chlorophyll, IAA, MDA and ABA contents was slowed down during the stress period while the rise/decline speed was accelerated during the recovery period. [ Conclusion] It suggests that the regulation of La ( III ) on membrane lipid peroxidation and endogenous hormones could increase chlorophyll and IAA contents, improve the metabolism of reactive oxygen species ( ROS), inhibit membrane lipid peroxidation, decrease the accumulation amount of ABA and alleviate injury of UV-B radiation to soybean seedlings. Further, the protective potential of La ( III ) was better under low UV-B radiation than under high one. 展开更多
关键词 La III UV-B radiation Soybean seedlings Membrane lipid peroxidation endogenous hormones
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Changes in Levels of Endogenous Plant Hormones During Floret Development in Wheat Genotypes of Different Spike Sizes 被引量:11
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作者 曹卫星 王兆龙 戴廷波 《Acta Botanica Sinica》 CSCD 2000年第10期1026-1032,共7页
The levels of endogenous plant hormones regulate floret development and degeneration, and thus grain set in flower crops. This study was undertaken to characterize the changes of endogenous hormone levels during flor... The levels of endogenous plant hormones regulate floret development and degeneration, and thus grain set in flower crops. This study was undertaken to characterize the changes of endogenous hormone levels during floret development in three wheat ( Triticum aestivum L.) genotypes: “97J1' with the highest grain set and fertile florets per spike, “H8679' with the lowest grain set and fertile florets per spike, and a medium, “YM158'. The results showed that the peak level of ABA appeared between stamen and pistil differentiation and antherlobe formation of floret development, and the timing delayed with the size of spike (earliest in “H8679” and latest in “97J1”). From antherlobe formation to meiosis, the levels of ABA and GA 1+3 decreased sharply in the ears of “97J1”, while in the ears of “H8679” there was only a slight decrease in ABA, and even an increase in GA 1+3 . The ratio of isopentenyladenosine (iPA)/ABA and IAA/ABA in the ears of “97J1” increased sharply from antherlobe formation to meiosis, but changed only slightly in the ears of “H8679”. At antherlobe formation, IAA and GA 1+3 levels were higher in the ears of “97J1”, but lower in the ears of “H8679” than in the leaves. At meiosis, ABA, GA 1+3 and IAA levels in the “97J1” ears were much lower than in the leaves, but similar in “H8679”. These results indicated that the sharp decreases of ABA and GA 1+3 in ears from antherlobe formation to meiosis and the lowest maintenance at meiosis may be favorable for development of fertile florets and enhancement of grain set in wheat. 展开更多
关键词 WHEAT endogenous hormones floret development genotypic difference large ear
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Effect of Paclobutrazol plus Ethephon Treatment on Endogenous Hormones and Carbon and Nitrogen Nutrients in Litchi Variety ‘Feizixiao' 被引量:5
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作者 陈炫 陶忠良 +4 位作者 吴志祥 王令霞 符惠珍 周兆德 范武波 《Agricultural Science & Technology》 CAS 2013年第8期1125-1131,共7页
[Objective] This study aimed to investigate the effect of spraying the mixture of paclobutrazol and ethephon on the endogenous hormones and carbon and nitrogen nutrients in litchi variety 'Feizixiao'.[Method] [Resul... [Objective] This study aimed to investigate the effect of spraying the mixture of paclobutrazol and ethephon on the endogenous hormones and carbon and nitrogen nutrients in litchi variety 'Feizixiao'.[Method] [Result] The results showed that foliar spraying of the mixture of paclobutrazol and ethephon could effectively inhibit litchi variety 'Feizixiao' to produce winter shoots,promote the flower bud differentiation and improve flower formation rate; meanwhile,this treatment could also increase the contents of ABA and ZR,and the ratios of ABA/IAA,ABA/GA3,ZR/IAA,ZR/GA3,and decrease the contents of IAA and GA3; additionally,it could increase the contents of soluble sugar,starch and total nitrogen,and raise C/N ratio,thus improving the flower formation rate.[Conclusion] Foliar spraying of the mixture of paclobutrazol and ethephon is an effective pathway for solving warmth damage to litchi. 展开更多
关键词 Litchi variety 'Feizixiao' Paclobutrazol and ethephon endogenous hormones Carbon and nitrogen nutrients
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Studies on Endogenous Hormones and Nutritional Physiology Related to the Premature Senescence of Super-hybrid Rice Liangyoupeijiu and Its Parents at Late Growth Stage 被引量:3
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作者 郭士伟 夏士健 +1 位作者 赵学强 朱虹霞 《Agricultural Science & Technology》 CAS 2014年第11期1914-1918,共5页
The amount of root bleeding sap, contents of chlorophyl , nutrients and hormones in flag leaves of a super-hybrid rice cultivar Liangyoupeijiu (LYPJ) and its parents 9311 and Pei’ai 64S after heading were measured ... The amount of root bleeding sap, contents of chlorophyl , nutrients and hormones in flag leaves of a super-hybrid rice cultivar Liangyoupeijiu (LYPJ) and its parents 9311 and Pei’ai 64S after heading were measured in this study. The re-sults revealed that compared with 9311, the chlorophyl content of LYPJ reduced more quickly after heading, and then kept at a lower level, which was an obvious characteristic of premature senescence. The other physiological indices of LYPJ af-ter heading except abscisic acid (ABA) content in leaf and root also maintained at a lower level than 9311, while al the physiological indices of the sterile line Pei'ai 64S were lower than LYPJ. So it was speculated that the early-aging characteristic of LYPJ may be inherited from Pei’ai 64S. Al the leaf and root early-aging traits reduced for LYPJ and its parent lines after heading, their leaf and root aged grad-ual y, which indicated that the above-ground (leaf) and under-ground (root) parts cor-related to each other closely, but there was not absolute correlations between them judged from the data. 展开更多
关键词 Super-hybrid rice Parent lines Premature senescence endogenous hormones Nutritional Physiology
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Relationship between Endogenous Hormones and Pod-length Development in Brassica napus L. 被引量:1
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作者 税红霞 汤天泽 牛应泽 《Agricultural Science & Technology》 CAS 2011年第5期710-714,共5页
[Objective] The aim was to study the relationship between endogenous hormone levels and the pod lengths in Brassica napus L.[Method] Two specially-long pod lines H218 and H203,two normal pod length lines MSP334,Zhongy... [Objective] The aim was to study the relationship between endogenous hormone levels and the pod lengths in Brassica napus L.[Method] Two specially-long pod lines H218 and H203,two normal pod length lines MSP334,Zhongyou 821 and two short pod lines "4101" and Zeng 11,were exploited as experimental materials,and the endogenous IAA,iPA,GA3 and ABA content in the pods of the materials were analyzed with the method of enzyme-linked immunosorbent assay(ELISA).[Result] IAA contents in the specially-long pod lines were lower than those in the short pod lines on the 1st and 5th day after blossom.IAA contents on the 5th and 20th day were negatively correlated to the final pod length respectively.iPA contents in the specially-long pod lines were lower than those in the short pod lines on the 5th and 20th day.iPA content on the first day was positively correlated to the final pod length,but iPA content on the fifth day,the tenth day and the fifteenth day were negatively correlated to the final pod length.GA3 content on the 1st day was positively correlated to the final pod length.ABA content on the 1st day was negatively correlated to the final pod length.[Conclusion] It was shown that iPA and IAA played important roles in the development of pod length in the B.napus lines. 展开更多
关键词 Brassica napus L. endogenous hormones Pod-length
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Relationship Between Heterosis and Endogenous Plant Hormones in Liriodendron 被引量:2
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作者 李周岐 王章荣 《Acta Botanica Sinica》 CSCD 2002年第6期698-701,共4页
To investigate the possible involvement of endogenous plant hormones in heterosis of Liriodendron interspecific hybrid, growth traits and contents of endogenous GA 1/3 (gibberellin A 1, A 3), IAA (indole_3_a... To investigate the possible involvement of endogenous plant hormones in heterosis of Liriodendron interspecific hybrid, growth traits and contents of endogenous GA 1/3 (gibberellin A 1, A 3), IAA (indole_3_acetic acid) and iPA (isopentenyl adenine) of tulip tree ( L. chinense (Hemsl.) Sarg.), yellow poplar ( L. tulipifera L.) and their interspecific hybrid ( L. chinense × L. tulipifera ) were examined. Results showed that: (1) the heterosis in height growth trait of the interspecific hybrid was mainly caused by the reletively greater elongation of internodes. Although the uppermost three internodes had the potential of elongation, the first one, which had the greatest elongation amount, contributed to the heterosis of the height growth trait; (2) the contents of endogenous GA 1/3 , IAA and iPA were greatly different among tulip tree, yellow poplar and their interspecific hybrid. All of the three interspecific hybrid families studied significantly contained higher amount of endogenous GA 1/3 and iPA in the uppermost first internode than their parental species. And thus, a correlation between the contents of endogenous GA 1/3 and iPA, and hybrid vigor for height growth trait in Liriodendron was observed; (3) the rankings of endogenous GA 1/3 and iPA contents in the uppermost first internode of three hybrid families studied were not similar to the ranking for height of 3_year_old trees. Therefore, the contents of endogenous GA 1/3 and iPA in the uppermost first internode could not be used in predicting hybrid vigor among hybrid families. 展开更多
关键词 LIRIODENDRON heterosis (hybrid vigor) endogenous plant hormone
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Effects of Drought Stress on Contents of Endogenous Hormones in Leaves of Different Clones of Liriodendron chinense×L. tulipifera
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作者 孙志勇 季孔庶 《Agricultural Science & Technology》 CAS 2010年第8期39-41,60,共4页
[Objective] The aim was to study the effects of drought stress on contents of endogenous hormones in leaves of different clones of Liriodendron chinense×L. tulipifera. Drought-resistant Liriodendron chinense×... [Objective] The aim was to study the effects of drought stress on contents of endogenous hormones in leaves of different clones of Liriodendron chinense×L. tulipifera. Drought-resistant Liriodendron chinense×L. tulipifera clones NE25,NE78 and drought-sensitive Liriodendron chinense×L. tulipifera clones NE04,NE23 were selected as experimental materials to study the content changes of four kinds of endogenous hormones such as abscisic acid (ABA),indoleacetic acid (IAA),ribosylzeatin (ZR) and jasmonic acid (JA) in leaves of different clones under drought stress. [Result] Under drought stress,ABA was accumulated I leaves of different clones,however,the content changes in drought-resistant clones were not obvious; IAA content showed a decreasing trend,and the content changes n drought-resistant clones during later period of drought stress was decreased; JA content showed an increasing trend in leaves of drought-sensitive clones,indicating that drought-sensitive clones had to maintain higher contents of JA to resist drought stress. [Conclusion] The result of this study had provided scientific basis for the screening,planting and application of drougt-resistant clones of Liriodendron chinense×L. tulipifera. 展开更多
关键词 Liriodendron chinense×L. tulipifera CLONE Drought stress endogenous hormone
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Effect of N Fertilizers on Root Growth and Endogenous Hormones in Strawberry 被引量:22
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作者 WANG Bo LAI Tao +2 位作者 HUANG Qi-Wei YANG Xing-Ming SHEN Qi-Rong 《Pedosphere》 SCIE CAS CSCD 2009年第1期86-95,共10页
Endogenous hormones play an important role in the growth and development of roots. The objective of this research was to study the effect of four types of N fertilizers on the root growth of strawberry (Fragaria anana... Endogenous hormones play an important role in the growth and development of roots. The objective of this research was to study the effect of four types of N fertilizers on the root growth of strawberry (Fragaria ananassa Duchesne) and the endogenous enzymes of indole-3-acetic acid (IAA), abscisic acid (ABA), and isopentenyl adenosine (iPA) in its roots and leaves using enzyme-linked immunosorbent assay. Application of all types of N fertilizers significantly depressed (P ≤ 0.05) root growth at 20 d after transplanting. Application of organic-inorganic fertilizer (OIF) as basal fertilizer had a significant negative effect (P ≤ 0.05) on root growth. The application of OIF and urea lowered the lateral root frequency in strawberry plants at 60 d (P ≤ 0.05) compared with the application of two organic fertilizers (OFA and OFB) and the control (CK). With the fertilizer treatments, there were the same concentrations of IAA and ABA in both roots and leaves at the initial growth stage (20 d), lower levels of IAA and ABA at the later stage (60 d), and higher iPA levels at all seedling stages as compared to those of CK. Thus, changes in the concentrations of endogenous phytohormones in strawberry plants could be responsible for the morphological changes of roots due to fertilization. 展开更多
关键词 endogenous hormone organic and inorganic N fertilizers root growth STRAWBERRY
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Response of root morphology, physiology and endogenous hormones in maize(Zea mays L.) to potassium deficiency 被引量:9
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作者 ZHAO Xin-hua YU Hai-qiu +4 位作者 WEN Jing WANG Xiao-guang DU Qi WANG Jing WANG Qiao 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2016年第4期785-794,共10页
Potassium (K) deficiency is one of the major abiotic stresses which has drastically influenced maize growth and yield around the world. However, the physiological mechanism of K deficiency tolerance is not yet fully... Potassium (K) deficiency is one of the major abiotic stresses which has drastically influenced maize growth and yield around the world. However, the physiological mechanism of K deficiency tolerance is not yet fully understood. To identify the differences of root morphology, physiology and endogenous hormones at different growing stages, two maize inbred lines 90-21-3 (tolerance to K deficiency) and D937 (sensitive to K deficiency) were cultivated in the long-term K fertilizer experimental pool under high potassium (+K) and low potassium (-K) treatments. The results indicated that the root length, volume and surface area of 90-21-3 were significantly higher than those of D937 under -K treatment at different growing stages. It was noteworthy that the lateral roots of 90-21-3 were dramatically higher than those of D937 at tasselling and flowering stage under-K treatment. Meanwhile, the values of superoxide dismutase (SOD) and oxidizing force of 90-21-3 were apparently higher than those of D937, whereas malondialdehyde (MDA) content of D937 was obviously increased. Compared with +K treatment, the indole-3-acetic acid (IAA) content of 90-21-3 was largely increased under-K treatment, whereas it was sharply decreased in D937. On the contrary, abscisic acid (ABA) content of 90-21-3 was slightly increased, but that of D937 was significantly increased. The zeatin riboside (ZR) content of 90-21-3 was significantly decreased, while that of D937 was relatively increased. These results indicated that the endogenous hormones were stimulated in 90-21-3 to adjust lateral root development and to maintain the physiology function thereby alleviating K deficiency. 展开更多
关键词 potassium deficiency MAIZE root morphology physiological variation endogenous hormone
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Effects of IBA on the rooting of branch cuttings of Chinese jujube (Zizyphus jujuba Mill.) and changes to nutrients and endogenous hormones 被引量:8
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作者 Fengxia Shao Sen Wang +1 位作者 Wen Huang Zhiming Liu 《Journal of Forestry Research》 SCIE CAS CSCD 2018年第6期1557-1567,共11页
‘Zhongqiusucui’ jujube secondary shoots were treated with 3-indolebutyric acid(IBA) at three concentrations, 500, 1000 and 1500 mg/L. Results show that IBA could significantly enhance rooting and root characteristic... ‘Zhongqiusucui’ jujube secondary shoots were treated with 3-indolebutyric acid(IBA) at three concentrations, 500, 1000 and 1500 mg/L. Results show that IBA could significantly enhance rooting and root characteristics of cuttings and were best with IBA at 1500 mg/L. In the rooting process, the formation of adventitious roots was related to the consumption and accumulation of nutrients(soluble sugars and proteins) and the changes in endogenous hormones in phloem, leaf tips and leaf bases. The rooting of cuttings had a positive correlation with the consumption of soluble sugars during the period of callus formation and with the accumulation of soluble sugars during adventitious root formation and growth. Rooting was positively related to the breakdown of soluble proteins in the phloem when the callus formed, and had a positive correlation with its accumulation during adventitious root formation and growth. Leaf tips and leaf bases showed a reverse trend in changes of soluble protein. However, together with the phloem, leaf tips and leaf bases regulated and controlled the formation and development of adventitious roots. The main activities of soluble proteins exist in the leaf tips as this was the main source of soluble proteins. The relation between rooting and IAA(indole-3-acetic acid) content in phloem was positive and thus a high concentration of IAA could benefit the induction and formation of adventitious roots. However, rooting was negatively related with ABA(abscisic acid) and GA(gibberellic acid) and a high concentration of both could inhibit the induction and formation of adventitious roots. Rooting had a positive correlation with phloem IAA/ABA ratios, and higher ratios could improve rooting. Low concentrations of ZR(zeatin riboside) triggered the induction of adventitious roots, while higher concentrations promoted root growth.Endogenous hormones in leaf tips and bases had an impact on rooting. The activities of endogenous hormones mainly existed in leaf tips because they play a major role in the production and consumption of IAA and its ABA content increased during rooting. The ZR in leaf tips influenced the rooting of cuttings, especially in the callus formation and rooting stage. Leaf tips were the main source of GA. 展开更多
关键词 Chinese jujube Ziziphus jujuba Mill. Rooting of cuttings Soluble sugars Soluble proteins endogenous hormones
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The Relation Between Endogenous Hormones and Late-Germination in Buds of Avrolles Apple 被引量:10
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作者 QIN Dong WANG Jin-zheng +1 位作者 GUO Jian-min ZHAI Heng 《Agricultural Sciences in China》 CAS CSCD 2009年第5期564-571,共8页
In order to provide the physiological bases for selecting late-germination cultivars that can avoid late frost damage, the very late-germination variety Avrolles (Malus domestica) was used to study the relation betw... In order to provide the physiological bases for selecting late-germination cultivars that can avoid late frost damage, the very late-germination variety Avrolles (Malus domestica) was used to study the relation between the dynamic changes and balance of endogenous hormones and germination time. The concentrations of endogenous GA3, ABA, IAA, and ZR were determined in buds of Avrolles and Judeline (Malus domestica) from dormancy releasing to germination by capillary electrophoresis. The dynamic changes of endogenous hormones concentration in buds of Avrolles and Judeline were similar; but the magnitude and time of the change were significantly different between the two varieties, especially for GA3. GA3 concentration increased with dormancy releasing, then decreased, and increased again before germination in the two varieties. GA3 concentration in Avrolles was 1.72 times that in Judeline at the first peak, the gap increased to 2.22 times at germination. ZR concentration exhibited a continuous increase trend, but it decreased sharply before germination. ZR accumulation in Avrolles took 36 days longer than in Judeline, the peak value was 44% higher than in Judeline. Before germination, ZR concentration in Avrolles was 2.12 times that in Judeline. The differences between IAA and ABA concentration were relatively small in the two varieties, while the ratios of GA3/ABA and (GA3 + IAA + ZR)/ABA in Avrolles were 2.08 and 1.58 times those in Judeline, respectively. The germination of apple bud was regulated by the endogenous hormones. For the late-germination apple Avrolles, its germination requires higher concentration of GA3 and ZR, which leads to the high ratios of GA3/ABA and (GA3+ IAA+ ZR)/ABA. 展开更多
关键词 Malus domestica cv. Avrolles Malus domestica cv. Judeline late-germination endogenous hormone
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Endogenous phytohormones and the expression of flowering genes synergistically induce flowering in loquat 被引量:4
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作者 CHI Zhuo-heng WANG Yong-qing +3 位作者 DENG Qun-xian ZHANG Hui PAN Cui-ping YANG Zhi-wu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2020年第9期2247-2256,共10页
Flowering is an important process for the reproduction of higher plants.Up to this point,the studies on flowering have mostly focused on the model plant Arabidopsis thaliana,and the flowering mechanism of fruit trees ... Flowering is an important process for the reproduction of higher plants.Up to this point,the studies on flowering have mostly focused on the model plant Arabidopsis thaliana,and the flowering mechanism of fruit trees remains mostly unknown.The diversity of the flowering time of loquat(Eriobotrya japonica Lindl.)makes it an ideal material to study the regulation of flowering.In this study,we first observed the inflorescence bud differentiation in two varieties of loquat that had different blooming times(cv.Dawuxing(E.japonica),that blooms in the fall and cv.Chunhua(E.japonica×Eriobotrya bengalensis Hook.f.)that blooms in the spring)and found that the starting time of inflorescence bud differentiation and the speed of inflorescence development were responsible for the difference in blooming times.The determination of endogenous phytohormones by high performance liquid chromatography(HPLC)indicated that abscisic acid(ABA),zeatin(ZT),and gibberellin(GA3)promoted flowering in loquat,while indole-3-acetic acid(IAA)was mainly involved in inflorescence bud differentiation in Chunhua.A transcription level analysis illustrated that multiple flowering-related genes could respond to different signals,integrate to the TFL1,AP1 and FT genes,and then synergistically regulate flowering in loquat.Thus,this study provides a new insight into flowering regulation mechanisms in loquat. 展开更多
关键词 LOQUAT inflorescence bud differentiation FLOWERING endogenous hormones gene expression
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The Influence of Endogenous Hormones on Culture Capability of Different Explants in Rice 被引量:2
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作者 WANGXiu-hong SHIXiang-yuan +2 位作者 WUXian-jun WANGXu-dong ZHOUKai-da 《Agricultural Sciences in China》 CAS CSCD 2005年第5期343-347,共5页
The endogenous hormones (EHs) content of different explants (anther, young panicle, young embryo and mature embryo) and calli with different culture capability were analyzed by means of high performance liquid chromat... The endogenous hormones (EHs) content of different explants (anther, young panicle, young embryo and mature embryo) and calli with different culture capability were analyzed by means of high performance liquid chromatography (HPLC). The results showed that the contents and ratio of endogenous hormones were one of the key factors affecting callus induction frequencies (CIF) and green plantlet differentiation frequencies (GPDF). The influence of endogenous hormones of different explants on CIF represented as: Zoatin ribosile (ZR) showed negative effect, indole-3-acetic acid (IAA) did positive effect, and gibberellic acid (GA) did negative effect except for mature embryos. The influence of endogenous hormones on green plantlet differentiation frequency (GPDF) showed: IAA and GA were negative effect; abscisic acid (ABA) and zeatin+ zeatin riboside (Z+ZR) were positive effect. The mixture ratio of endogenous hormones played a role on CIF and GPDF. IAA/Z+ZR had a positive effect on CIF, and there was a notable positive correlation between Z+ZR/ IAA and GPDF, so was between ABA/IAA and GPDF. 展开更多
关键词 RICE endogenous hormones (EHs) EXPLANT Callus induction frequency (CIF) Green plantlet differentiation frequency (GPDF)
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Content Changes of Several Endogenous Plant Hormones in Female-Sterile Pinus tabulaeformis Carr. 被引量:3
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作者 Bao Ren-yan Zheng Cai-xia 《Forestry Studies in China》 CAS 2005年第4期16-19,共4页
To determine the relationship between female sterility of Pinus tabulaeformis Carr. and plant hormones, content changes of several endogenous plant hormones in a female-sterile clone and a normal clone were analyzed d... To determine the relationship between female sterility of Pinus tabulaeformis Carr. and plant hormones, content changes of several endogenous plant hormones in a female-sterile clone and a normal clone were analyzed during the key period of female gametophyte abortion in mutant clones. The change of ABA content showed a similar pattern between normal and mutant clones, but the ABA content of the mutant clone was always much higher than that of the normal clone. In the normal clone, the IAA content decreased significantly at the early stage of the period and reached its minimum during the first ten days of April and stabilized thereafter. The endogenous ZR levels increased sharply at the early stages and reached a maximum in the middle of April, then decreased dramatically. The IAA and ZR contents of the mutant clone did not change perceptively through the entire period. We conclude that the high levels of ABA and the deficit of IAA and ZR may be the reasons of female gametophyte abortion in the female-sterile clones of P. tabulaeformis. 展开更多
关键词 endogenous hormones female sterility Pinus tabulaeformis Carr.
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Correlation Between Endogenous Hormones of Stem Apices and Fruit Locule Numbers in Tomatoes During Floral Bud Differentiation Stages 被引量:2
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作者 LI Yue LI Tian-lai WANG Dan 《Agricultural Sciences in China》 CAS CSCD 2008年第4期447-454,共8页
The study was designed to elucidate the changes in the endogenous hormones of stem apices in tomatoes and the function of correlative endogenous hormones in tomatoes during floral bud differentiation stages. The tomat... The study was designed to elucidate the changes in the endogenous hormones of stem apices in tomatoes and the function of correlative endogenous hormones in tomatoes during floral bud differentiation stages. The tomato parents were crossed and reverse crossed by using two inbred lines of multi-locule (MLK1) and few-locule (FL1) with significant difference, and the relationship between endogenous hormones GA3, IAA, and ABA levels and ovary locule numbers of parents and progeny during floral bud differentiation initial stage, floral bud differentiation stage, sepal petal formation stage, carpel formation initial stage, and ovary locule complete formation stage was studied. GA3 levels in P1, P2, F1, and RF1 were consistent with locule numbers, and IAA and ABA levels were reverse to ovary locule numbers during the key stage. The correlation showed that, during sepal petal formation stage, the ovary locule numbers were positively correlated with GA3, GA3/IAA, and GA3/ABA, and were negatively correlated with IAA and ABA. It was speculated that increasing GA3 levels or decreasing IAA and ABA levels of stem apices in tomato might be able to enhance ovary locule numbers. The sepal petal formation stage was an important stage which regulated endogenous hormones in the ovary locule formation. 展开更多
关键词 TOMATO endogenous hormones locule numbers CORRELATION
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The Use of HPLC in Determination of Endogenous Hormones in Anthers of Bitter Melon 被引量:4
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作者 Yi Tang Li Wang Chao Ma Ji Liu Bin Liu Huanxiu Li 《Journal of Life Sciences》 2011年第2期139-142,共4页
A simple, rapid and perfect extraction and determination method for the endogenous hormones in anthers of bitter melon with reversed-phase high performance liquid chromatography (HPLC) has been developed. The HPLC s... A simple, rapid and perfect extraction and determination method for the endogenous hormones in anthers of bitter melon with reversed-phase high performance liquid chromatography (HPLC) has been developed. The HPLC system consisted of Hypersil ODS C 18 reverse phase column (150 mm × 4.6 mm, 5 μm) with a methanol gradient in 0.6% acetic acid and UV detector set at 254 nm. The results showed that the method was accurate and efficient. 展开更多
关键词 HPLC endogenous hormones bitter melon (Momordica eharantia L.)
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Effects of chilling tolerance induced by spermidine pretreatment on antioxidative activity,endogenous hormones and ultrastructure of indica-japonica hybrid rice seedlings 被引量:4
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作者 ZENG Yan-hua ZAHNG Yu-ping +4 位作者 XIANG Jing WU Hui CHEN Hui-zhe ZHANG Yi-kai ZHU De-feng 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2016年第2期295-308,共14页
Spermidine(Spd) is known to be involved in the regulation of plant responses to chilling stress and counteract the adverse effect of stress conditions.Antioxidant activities,endogenous hormones and ultrastructure ch... Spermidine(Spd) is known to be involved in the regulation of plant responses to chilling stress and counteract the adverse effect of stress conditions.Antioxidant activities,endogenous hormones and ultrastructure change under chilling stress were investigated in indica-japonica hybrid rice seedlings.12-d-old seedlings were subjected to exogenous Spd(1 mmol L^(-1)) and then a chilling stress(6℃,4 d) was induced,followed by a subsequent recovery(25℃,4 d).Results showed that malondialdehyde(MDA) and proline content were enhanced significantly,whereas shoot fresh and dry weights decreased during chilling stress and after recovery;chlorophyll content of chilling-stressed seedlings increased slightly but declined after recovery;additionally,total soluble sugar,sucrose,fructose and starch contents increased significantly during chilling stress,and only soluble sugar and fructose contents were observed in increase after recovery;chilling stress-induced increases in superoxide dismutase(SOD),peroxidase(POD) and catalase(CAT) activities,but declined after recovery,and the level of ascorbate peroxidase was lower during chilling stress and after recovery;however,endogenous indole-3-acetic acid(IAA),zeatin riboside(ZR),gibberellic acid(GA_3),and abscisic acid(ABA) levels were induced decreased compared with Spd pretreatment.The microscopic analysis revealed that chilling stress-induced destruction of the chloroplast envelope during chilling stress and increased the number of plastoglobuli along with aberrations in thylakoid membranes after recovery.In contrast,exogenous Spd protected rice seedlings from chilling-induced injuries in terms of lower malondialdehyde,proline and carbohydrates accumulation coupled with increased endogenous hormones metabolism.After recovery,Spd pretreatment chilling-exposed seedlings showed higher activities of antioxidant enzymes and normal physiological function of chloroplasts.These results suggest that Spd could promote effectively chilling tolerance which might be largely attributable to the integrity of cell structure and normal metabolism of endogenous hormones in indica-japonica hybrid rice seedlings. 展开更多
关键词 polyamines chilling stress antioxidative activity endogenous hormones ultrastructure indica-japonica hybrid rice(Oryza sativa L.)
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Effects of Root Cutting on Morphological Characteristics and Endogenous Hormone Levels of Quercus variabilis Seedlings 被引量:1
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作者 Ruoyi Ni Hao Dou +3 位作者 Shuwen Bi Tiantian Wang Jin’e Quan Xitian Yang 《Phyton-International Journal of Experimental Botany》 SCIE 2023年第10期2907-2920,共14页
The objective of this study was to investigate the effects of root cutting stress on the dynamic changes of endogenous hormone content and growth characteristics of Quercus variabilis roots,and to explore the physiolo... The objective of this study was to investigate the effects of root cutting stress on the dynamic changes of endogenous hormone content and growth characteristics of Quercus variabilis roots,and to explore the physiological role of endogenous hormones in regulating root-crown interactions in the short term.The morphological characteristics and endogenous hormone contents of normal roots(no root cutting,CK)and cut roots(cut by 1/3 of the length of the main root,RP)were determined by liquid chromatography,which was combined with mass spectrometry at different levels of different developmental stages.The results showed that the root growth indexes and root endogenous hormones in the RP group were superior to those in the CK group.Through comprehensive analysis of endogenous hormones,it was found that the crosstalk of IAA,JA,ABA and SA could activate the root growth defense.After the root cutting treatment,the root growth of Quercus variabilis seedlings could compensate for the inhibition of taproot growth by promoting lateral root growth.The growth and development of compensatory lateral roots contribute to the increase the total root length of plants,thus promoting the absorption of water and nutrients.It is speculated that plant hormones may be the key factors affecting their development,but this is not only related to the content of a single hormone,but more importantly,it is the interaction between various hormones. 展开更多
关键词 Quercus variabilis root cutting injury endogenous hormones root morphology
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Separation and Measurement of Three Kinds of Endogenous Hormones in Rhizome of Alhagi sparsifolia
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作者 ZHAO Man-li YANG Ying-chun +1 位作者 YANG Shi-jie YUAN Zhen-yan 《Animal Husbandry and Feed Science》 CAS 2012年第3期142-144,共3页
[ Objective] The aim was to establish effective method for endogenous hormone extraction and explore conditions of chromatographic analysis for three endogenous hormones in rhizome of Alhagi sparsifolia. [ Methed ] Ac... [ Objective] The aim was to establish effective method for endogenous hormone extraction and explore conditions of chromatographic analysis for three endogenous hormones in rhizome of Alhagi sparsifolia. [ Methed ] Activol (GA3 ), zeatin (ZR) and indole-3-acetic acid (IAA) in rhizome were separated and measured as per RP-HPLC. [Result] The average recovery rates of GA3, ZR and IAA were 98.3%, 90.3% and 101.3%, respectively, indicating that the method is suitable for quantitative analysis with little errors. The chromatographic conditions were as follows: methanol/0. 75% of acetic acid at 45:55 (mobile phase) ; flow speed at 0.7 ml/min; wavelength at 254 nm; column temperature at 25 ℃. [Conclusion] The research preliminarily established HPLC conditions for separation of endogenous hormones in rhizome of Alhagi sparsifolia. 展开更多
关键词 Alhagi sparsifolia endogenous hormones Reversed-phase high performance liquid chromatography (RP-HPLC)
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Dynamic Changes of Endogenous Hormone Content in Dangshansu Pear during Fruit Development 被引量:2
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作者 张昂 徐义流 +4 位作者 潘海发 张金云 高正辉 束冰 郭传翔 《Agricultural Science & Technology》 CAS 2011年第7期940-942,962,共4页
[Objective] The aim was to clear the relationship between fruit growth and development and endogenous hormones content in the pulp of Dangshansu pear.[Method] Choosing Dangshansu pear as material,the dynamic changes o... [Objective] The aim was to clear the relationship between fruit growth and development and endogenous hormones content in the pulp of Dangshansu pear.[Method] Choosing Dangshansu pear as material,the dynamic changes of endogenous hormones in pulp during various development stages were determined by means of enzyme linked immunosorbent assay(ELISA).[Result] The results indicated that the content of endogenous hormones like ZR,GA and IAA promoting fruit growth showed increase trend within 15 d after pollination,and higher content of ZR,GA and IAA could promote ovary development and normal fruit setting of Dangshansu pear.The content of ZR in pulp had the highest value at the beginning of the first fast growth period,and then showed decrease trend until fruit became mature.The dynamic changes of IAA and GA were similar,namely their contents were higher in the first fast growth period,and decreased with fruit development,and then increased rapidly and was up to the maximum value in the second fast growth period,corresponding with the growth dynamic of fruit.The peaks of ABA content could be found on the 15th,45th and 120th day after pollination,which corresponded with the physiological fruit dropping and maturing period of Dangshansu pear.[Conclusion] The duration of various development periods and content of endogenous hormones of Dangshansu pear were different,and fruit growth and development were closely related to the content of endogenous hormones. 展开更多
关键词 Dangshansu pear endogenous hormones Fruit development
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