Objective To investigate the influence of endogenous transforming growth factor β1(TGFβ1) on the cell cycle regulation and proliferation of bladder cancer. Methods A constructed replication defective retroviral vect...Objective To investigate the influence of endogenous transforming growth factor β1(TGFβ1) on the cell cycle regulation and proliferation of bladder cancer. Methods A constructed replication defective retroviral vector pRevTβ-AS, which carried antisense RNA of TGFβ1.was transfected to a bladder cancer cell line EJ. The proliferation and clone-formation of transferred cells were observed in vitro,and the alteration of cell cycle was also detected by flow cytometric analysis. Results TGFβ1 antisense RNA was transferred into EJ cell and expressed efficiently. After the inhibition of target gene expression in EJ cells, the reduced growth and clone-formation rates were demonstrated, and the proliferative indexes were decreased by 12 % . The ratios of GO and G1 stage cells to June 2003 Vol12 No2 the antisense RNA-transfected EJ cells were increased, simultaneously,the ratio of S stage cells to the antisense RNA-transfected EJ cells ratios were decreased, compared with the control group. Conclusion The展开更多
Weibo,China’s largest microblogging platform,has become one of the key information-sharing platforms in modern society.This study examines topic propagation in relation to microblogging from the perspective of the“p...Weibo,China’s largest microblogging platform,has become one of the key information-sharing platforms in modern society.This study examines topic propagation in relation to microblogging from the perspective of the“peer effect.”Using data of hot topics from Weibo,we analyze how the social effect and propagation pathway influence the topic propagation process.We propose a spatial and temporal heterogeneity diffusion model that includes endogenous and exogenous social effects and is based on but different from the Bass diffusion model.We find that most propagation pathways end after a single level of propagation.The endogenous social effect in microblogs primarily influences the inflow of topics.Such endogenous social effect,combined with the multiplier effect,motivates most users to share a microblog topic in a short period of time.The exogenous social effect primarily influences the outflow of topics,and therefore,the microblog topics of a small number of popular users’account for most of the share volume.Our results are robust to potential serial correlation,reflection problem,and potential self-selection due to user status.The findings reveal that group characteristics affect individuals’behaviors and choices in relation to the topic propagation process on microblogging platforms.The use of a spatial and temporal heterogeneity diffusion model and the robustness of the analysis process provide new information for scholars in this field.展开更多
文摘Objective To investigate the influence of endogenous transforming growth factor β1(TGFβ1) on the cell cycle regulation and proliferation of bladder cancer. Methods A constructed replication defective retroviral vector pRevTβ-AS, which carried antisense RNA of TGFβ1.was transfected to a bladder cancer cell line EJ. The proliferation and clone-formation of transferred cells were observed in vitro,and the alteration of cell cycle was also detected by flow cytometric analysis. Results TGFβ1 antisense RNA was transferred into EJ cell and expressed efficiently. After the inhibition of target gene expression in EJ cells, the reduced growth and clone-formation rates were demonstrated, and the proliferative indexes were decreased by 12 % . The ratios of GO and G1 stage cells to June 2003 Vol12 No2 the antisense RNA-transfected EJ cells were increased, simultaneously,the ratio of S stage cells to the antisense RNA-transfected EJ cells ratios were decreased, compared with the control group. Conclusion The
基金the National Natural Science Foundation of China(Grant Nos.70801066,71071167,7107168,71371200)Sun Yat-sen University Basic Research Funding(Grant Nos.1009028,1109115,16wkjc13)。
文摘Weibo,China’s largest microblogging platform,has become one of the key information-sharing platforms in modern society.This study examines topic propagation in relation to microblogging from the perspective of the“peer effect.”Using data of hot topics from Weibo,we analyze how the social effect and propagation pathway influence the topic propagation process.We propose a spatial and temporal heterogeneity diffusion model that includes endogenous and exogenous social effects and is based on but different from the Bass diffusion model.We find that most propagation pathways end after a single level of propagation.The endogenous social effect in microblogs primarily influences the inflow of topics.Such endogenous social effect,combined with the multiplier effect,motivates most users to share a microblog topic in a short period of time.The exogenous social effect primarily influences the outflow of topics,and therefore,the microblog topics of a small number of popular users’account for most of the share volume.Our results are robust to potential serial correlation,reflection problem,and potential self-selection due to user status.The findings reveal that group characteristics affect individuals’behaviors and choices in relation to the topic propagation process on microblogging platforms.The use of a spatial and temporal heterogeneity diffusion model and the robustness of the analysis process provide new information for scholars in this field.
