Development and validation of a circulating tumor DNA-based optimization-prediction model for short-term postoperative recurrence of endometrial cancer

BACKGROUND Endometrial cancer(EC)is a common gynecological malignancy that typically requires prompt surgical intervention;however,the advantage of surgical management is limited by the high postoperative recurrence rates and adverse outcomes.Previous studies have highlighted the prognostic potential of circulating tumor DNA(ctDNA)monitoring for minimal residual disease in patients with EC.AIM To develop and validate an optimized ctDNA-based model for predicting shortterm postoperative EC recurrence.METHODS We retrospectively analyzed 294 EC patients treated surgically from 2015-2019 to devise a short-term recurrence prediction model,which was validated on 143 EC patients operated between 2020 and 2021.Prognostic factors were identified using univariate Cox,Lasso,and multivariate Cox regressions.A nomogram was created to predict the 1,1.5,and 2-year recurrence-free survival(RFS).Model performance was assessed via receiver operating characteristic(ROC),calibration,and decision curve analyses(DCA),leading to a recurrence risk stratification system.RESULTS Based on the regression analysis and the nomogram created,patients with postoperative ctDNA-negativity,postoperative carcinoembryonic antigen 125(CA125)levels of<19 U/mL,and grade G1 tumors had improved RFS after surgery.The nomogram’s efficacy for recurrence prediction was confirmed through ROC analysis,calibration curves,and DCA methods,highlighting its high accuracy and clinical utility.Furthermore,using the nomogram,the patients were successfully classified into three risk subgroups.CONCLUSION The nomogram accurately predicted RFS after EC surgery at 1,1.5,and 2 years.This model will help clinicians personalize treatments,stratify risks,and enhance clinical outcomes for patients with EC.

2020款比亚迪秦EV高压互锁结构原理及故障诊断

介绍2020款比亚迪秦EV高压互锁结构和原理,基于结构和原理,分析和排除实车故障,分享整体检修思路和过程,以便体现掌握高压互锁结构和原理对故障检修的重要性。

灰树花水溶物对EV71病毒的抑制作用

【目的】探讨灰树花水溶物(GFWE)的化学成分及其对EV71病毒的抑制作用,为促进灰树花综合利用、提高其经济附加值,以及开发预防和治疗EV71病毒感染的功能性食品提供依据。【方法】采用色谱法测定了GFWE的主要化学成分组成、多糖组成和分子质量。基于体外细胞培养采用CCK-8法确定了GFWE的安全上样量;以EV71病毒感染Vero细胞为模型,探究GFWE对EV71病毒的抑制率及对Vero细胞形态的影响,并采用荧光定量PCR检测了EV71病毒衣壳蛋白(VP1)编码基因mRNA的表达水平,初步评价了GFWE对EV71病毒的抑制作用。【结果】GFWE主要由低分子质量多糖、氨基酸、肽及其衍生物组成。GFWE富含的13种氨基酸中包含7种必需氨基酸;其多糖由葡萄糖、半乳糖、盐酸氨基葡萄糖和古罗糖醛酸4种单糖组成,物质的量比为0.907∶0.038∶0.029∶0.026,重均分子质量分别为15.67、10.10、6.60 ku和<1.00 ku。GFWE表现出较高的抗EV71病毒活性,100、200μg·mL^(-1) GFWE对EV71病毒的抑制率分别为91.32%、91.83%,25~400μg·mL^(-1) GFWE能显著降低EV71 VP1 mRNA的表达水平。【结论】GFWE主要由低分子质量多糖、氨基酸、肽及其衍生物组成,具有较高的抗EV71病毒活性,能够抑制感染EV71病毒的Vero细胞中VP1的合成。

考虑EV充放电意愿的园区综合能源系统双层优化调度

随着电动汽车(electric vehicle,EV)普及度的不断提高,工业园区内的EV用户日益增多,其充放电行为给园区综合能源系统(park integrated energy system,PIES)的规划运行带来极大挑战。文中提出考虑EV充放电意愿的PIES双层优化调度。首先,基于动态实时电价、电池荷电量、电池损耗补偿、额外参与激励等因素建立充放电意愿模型,在此基础上得到改进的EV充放电模型;然后,以PIES总成本最小和EV充电费用最小为目标建立双层优化调度模型,通过Karush-Kuhn-Tucker(KKT)条件将内层模型转化为外层模型的约束条件,从而快速稳定地实现单层模型的求解;最后,进行仿真求解,设置3种不同场景,对比所提模型与一般充放电意愿模型,验证了文中所提引入EV充放电意愿模型的PIES双层优化调度的有效性和可行性。

Value of Intrauterine Autologous Platelet-Rich Plasma Therapy on Endometrial Receptivity:A Literature Review

Endometrial receptivity is an important factor that influences embryo implantation.Thus,it is important to identify an applicable approach to improve endometrial receptivity in women undergoing assisted reproductive technology.Recently,growing evidence has indicated that intrauterine platelet-rich plasma(PRP)infusion is an effective method to obtain a satisfactory reproductive outcome by increasing endometrial thickness and improving endometrial receptivity.Therefore,the present review aims to outline the possible mechanisms of PRP on endometrial receptivity and summarize the present literature on the effects of PRP therapy in improving endometrial receptivity.

基于多胞体系统的FWID EV鲁棒加权增益调度轨迹跟踪控制

为了提高四轮独立驱动电动汽车(FWID EV)轨迹跟踪控制性能,针对车辆横向运动系统的不确定性与非线性问题,考虑转向执行机构的动态特性,提出了基于线性变参数(LPV)的鲁棒加权增益调度轨迹跟踪控制策略。首先利用凸分解技术将LPV系统转化为有限多胞顶点凸组合的多胞体系统,然后基于离线设计的每个多胞顶点控制器和线性组合在线计算任意时变参数对应的系统控制器参数,并利用MATLAB和CarSim进行联合仿真。结果表明:在加速变道场景下,所设计的控制器可以保证横向偏移误差不超过2 cm,横向偏移平均绝对误差不超过0.9 cm的较高跟踪精度及行驶稳定性和鲁棒性。

circRNA3669 promotes goat endometrial epithelial cells proliferation via miR-26a/RCN2 to activate PI3K/AKT-mTOR and MAPK pathways

The development of receptive endometrium(RE) from pre-receptive endometrium(PE) for successful embryo implantation is a complex dynamic process in which the morphology and physiological states of the endometrial epithelium undergo a series of significant changes, including cell proliferation and apoptosis. However, the molecular mechanisms are not yet fully understood. In this study, a higher circRNA3669 level was observed in PE than in RE of goats. Functional assays revealed that this overexpression promoted the proliferation of goat endometrial epithelial cells(GEECs) by activating the expression of genes related to the PI3K/AKT-mTOR and MAPK pathways,thereby inhibiting apoptosis in vitro. Furthermore, circRNA3669 functioned as a competing endogenous RNA(ceRNA) to upregulate Reticulocalbin-2(RCN2) expression at the post-transcriptional level by interacting with and downregulating miR-26a in GEECs. In addition, RCN2, which is highly expressed in the PE of goats, was found to be regulated by β-estradiol(E2) and progesterone(P4). Our results demonstrated that RCN2 also affected the key proteins PI3K, AKT, mTOR, JNK, and P38 in the PI3K/AKT-mTOR and MAPK pathways, thereby facilitating GEECs proliferation and suppressing their apoptosis in vitro. Collectively, we constructed a new circRNA3669-miR-26aRCN2 regulatory network in GEECs, which further provides strong evidence that circRNA could potentially play a crucial regulatory role in the development of RE in goats.

EVS系统在演播室体育赛事转播中的应用

基于增强语音服务(Enhanced Voice Services, EVS)在体育赛事转播中的应用,对其系统组成、组织架构和应用场景等进行深入研究,以期提高演播室体育赛事转播的质量和效率。

ZNF554 Inhibits Endometrial Cancer Progression via Regulating RBM5 and Inactivating WNT/β-Catenin Signaling Pathway

Objective:Uterine corpus endometrial carcinoma(UCEC),a kind of gynecologic malignancy,poses a significant risk to women’s health.The precise mechanism underlying the development of UCEC remains elusive.Zinc finger protein 554(ZNF554),a member of the Krüppel-associated box domain zinc finger protein superfamily,was reported to be dysregulated in various illnesses,including malignant tumors.This study aimed to examine the involvement of ZNF554 in the development of UCEC.Methods:The expression of ZNF554 in UCEC tissues and cell lines were examined by qRT-PCR and Western blot assay.Cells with stably overexpressed or knocked-down ZNF554 were established through lentivirus infection.CCK-8,wound healing,and Transwell invasion assays were employed to assess cell proliferation,migration,and invasion.Propidium iodide(PI)staining combined with fluorescence-activated cell sorting(FACS)flow cytometer was utilized to detect cell cycle distribution.qRT-PCR and Western blotting were conducted to examine relative mRNA and protein levels.Chromatin immunoprecipitation assay and luciferase reporter assay were used to explore the regulatory role of ZNF554 in RNA binding motif 5(RBM5).Results:The expression of ZNF554 was found to be reduced in both UCEC samples and cell lines.Decreased expression of ZNF554 was associated with higher tumor stage,decreased overall survival,and reduced disease-free survival in UCEC.ZNF554 overexpression suppressed cell proliferation,migration,and invasion,while also inducing cell cycle arrest.In contrast,a decrease in ZNF554 expression resulted in the opposite effect.Mechanistically,ZNF554 transcriptionally regulated RBM5,leading to the deactivation of the Wingless(WNT)/β-catenin signaling pathway.Moreover,the findings from rescue studies demonstrated that the inhibition of RBM5 negated the impact of ZNF554 overexpression onβ-catenin and p-glycogen synthase kinase-3β(p-GSK-3β).Similarly,the deliberate activation of RBM5 reduced the increase inβ-catenin and p-GSK-3βcaused by the suppression of ZNF554.In vitro experiments showed that ZNF554 overexpression-induced decreases in cell proliferation and migration were counteracted by RBM5 knockdown.Additionally,when RBM5 was overexpressed,it hindered the improvements in cell proliferation and migration caused by reducing the ZNF554 levels.Conclusion:ZNF554 functions as a tumor suppressor in UCEC.Furthermore,ZNF554 regulates UCEC progression through the RBM5/WNT/β-catenin signaling pathway.ZNF554 shows a promise as both a prognostic biomarker and a therapeutic target for UCEC.

Endometrial carcinoma with cervical stromal invasion:Three case reports

BACKGROUND Endometrial cancer is a kind of well-known tumors of female genitourinary system.Cervical stromal invasion is an adverse factor for poor prognosis of endometrial cancer.There is still controversy regarding the use of magnetic resonance imaging(MRI)in the diagnosis of cervical stromal invasion of endometrial cancer.The diagnosis of cervical stromal invasion varies significantly between different observers and institutions.We present a limited case series of the particular pattern of endometrial cancer,which infiltrates the cervical stroma and is often overlooked.CASE SUMMARY We present three cases of endometrial carcinoma with cervical stromal invasion with cancer-free uterine cavity.One patient,a reproductive-aged woman,exhibited irregular menstruation and was diagnosed with endometrial polyps by hysteroscopy and segmental curettage.A MRI scan revealed polypoid nodules within the internal cervical orifice.The other two cases were postmenopausal women who presented with abnormal vaginal bleeding.Hysteroscopy and segmental curettage suggested atypical hyperplasia of the endometrium.MRI scans did not detect any malignant signs in the endometrium.In one case,a nonthickened endometrium was observed,while in another,hyperplasia of the endometrium was seen.Notably,none of these patients had malignant tumors identified in the uterine cavity via MRI scans.However,postoperative pathological results following hysterectomy consistently indicated cervical stromal invasion.CONCLUSION Cervical stromal invasion is easily missed if no cancer is found in the uterine body on MRI.Immunohistochemistry of endoscopic curettage specimens should be conducted to avoid underestimation of the disease.

Edge Impulse Based ML-Tensor Flow Method for Precise Prediction of Remaining Useful Life (RUL) of EV Batteries

Electric Vehicle (EV) adoption is rapidly increasing, necessitating efficient and precise methods for predicting EV charging requirements. The early and precise prediction of the battery discharging status is helpful to avoid the complete discharging of the battery. The complete discharge of the battery degrades its lifetime and requires a longer charging duration. In the present work, a novel approach leverages the Edge Impulse platform for live prediction of the battery status and early alert signal to avoid complete discharging. The proposed method predicts the actual remaining useful life of batteries. A powerful edge computing platform utilizes Tensor Flow-based machine learning models to predict EV charging needs accurately. The proposed method improves the overall lifetime of the battery by the efficient utilization and precise prediction of the battery status. The EON-Tuner and DSP processing blocks are used for efficient results. The performance of the proposed method is analyzed in terms of accuracy, mean square error and other performance parameters.

比亚迪秦EV低压无法上电故障分析

随着国家对新能源汽车产业支持,新能源汽车保有量不断升高,汽车故障率随之提升。文章以市场保有量较高的比亚迪秦EV车型为例,主要介绍低压上电工作原理和常见故障。在诊断过程中,严谨的诊断思路对于维修人员极为重要,文章从故障现象描述、分析故障可能原因,诊断步骤,故障机理分析四个方面进行详细说明。通过分析,建立起诊断思路,为职业院校师生和维修从业人员在维修教学过程中提供一定的参考信息。

一种适用于EV纯电动乘用车的轮胎子口结构

由青岛双星轮胎工业有限公司申请的专利(公布号CN 114872496A,公布日期2022-08-09)“一种适用于EV纯电动乘用车的轮胎子口结构”,公开了一种适用于纯电动乘用车的轮胎胎圈结构,包括钢丝圈、设于钢丝圈上端的三角胶和胎体,其中胎体包括一层胎体、胎圈耐磨胶和胎侧胶;一层胎体末端内端点和三角胶交汇处形成一层胎体反包端点;以胎圈耐磨胶和胎侧胶两者相接处下端的胎圈耐磨胶下端点为原点,向三角胶做距离最近的垂线得胎圈耐磨胶下端点法向点.

吉利帝豪EV450无法交流慢充故障诊断与排除

吉利帝豪EV450交流慢充系统控制着动力电池组到充电桩之间能量的传输,通过CC以及CP的数据传输,确保充电枪的准确连接,同时通过BMS对充电电流、电压、温度、绝缘情况等精准监控,保证了交流慢充的正常进行。本文详细介绍了EV450的交流慢充系统无法充电的故障排除,从构造组成、工作模式及详细的故障检修过程进行阐述。

EV-A71疫苗接种后手足口病流行及临床特点

目的探讨肠道病毒71型(EV-A71)疫苗接种后手足口病的流行及临床特点。方法收集2018年1月至2021年12月杭州市儿童医院688例肠道病毒感染手足口病患儿的临床资料,分析EV-A71疫苗接种史的手足口病的病原学及临床特点。结果共有EV-A71疫苗接种史手足口病患儿47例(接种一次疫苗11例,接种二次疫苗36例)。其中男28例,女19例;年龄8个月~4岁。<1岁5例,1~3岁40例,>3~4岁1例。临床表现:47例均发热,口、咽、手、足和臀部出疹分别47例、36例、33例、32例;颜面、颈部、四肢、躯干、肘部等其他部位出皮疹25例。47例疫苗后手足口病中,肠道病毒A6型(CV-A6)感染31例,肠道病毒A16型(CV-A16)感染9例,其他型肠道病毒感染7例,无EV-A71感染。血清EV-A71 IgM抗体阳性14例,其中阳性3例,弱阳性11例,接种1次EV-A71疫苗6例,接种2次EV-A71疫苗8例,接种与发病时间最长12个月。结论全面接种EV-A71疫苗能有效预防EV-A71感染,建议积极推广。

EVS三维地质建模技术在某地铁工程中的应用

EVS(earth volumetric studio)三维地质模型可为城市规划建设和资源评估计算等提供数据支持,在轨道交通领域同样产生积极影响。为解决浅部地层建模出现不准确区域的问题,以某地铁工程实测钻孔为例,采用调整Pinch Factor和层序优化的方法,并对建模成果进行误差验证。结果表明:优化后的地质模型相比原模型,平均相对误差和均方根误差分别降低了2.40%~21.20%、0.10~0.72 m,优化后的模型精度更高。其应用分析可为同类工程建设提供参考。

EV408智能搅拌车无法自动驾驶故障诊断与分析

文章重点介绍EV408智能搅拌车无法自动驾驶的故障现象、故障原因和故障诊断与排除的方法。通过分析其故障诊断的原因,并结合实训操作,介绍诊断故障的基本检测流程。

吉利帝豪EV450无法上高压的故障诊断与排除

本文阐述了一辆帝豪EV450的纯电动汽车,由于高压互锁故障而导致整车不能上高压的故障。通过对高压系统的结构及高压系统各监测控制原理以及相关检测知识点的分析,查阅维修手册对故障排除的过程。

吉利帝豪EV450常见电控故障诊断与排除

该文针对吉利帝豪EV450常见电控故障,如室内天线故障、CAN信号线故障、制动踏板故障、启动按钮故障、仪表故障、高压互锁故障等,结合该车的系统结构、电路图与控制原理,对这些故障进行诊断与排除,以保证电动汽车行驶的安全性和稳定性。

吉利帝豪EV450汽车高压互锁系统故障定位与维修研究

随着电动汽车的快速发展,高压互锁作为确保车辆安全运行的关键系统之一,其工作原理和故障定位与维修的研究显得尤为重要。该文选择吉利帝豪EV450这款具有代表性的电动汽车作为研究对象,介绍高压互锁系统常见故障类型,探讨高压互锁系统故障定位方法,并提出高压互锁系统故障维修建议,以提高维修效率,消除电动汽车运行过程中的安全隐患。