Metastasis of colorectal adenocarcinoma of the ovary is not an uncommon occurrence and ovarian metastases from colorectal carcinoma frequently mimic endometrioid and mucinous primary ovarian carcinoma.The clinical and...Metastasis of colorectal adenocarcinoma of the ovary is not an uncommon occurrence and ovarian metastases from colorectal carcinoma frequently mimic endometrioid and mucinous primary ovarian carcinoma.The clinical and pathologic features of metastatic colorectal adenocarcinoma involving the ovary is reviewed with particular focus on the diagnostic challenge of distinguishing these secondary ovarian tumors from primary ovarian neoplasm.Immunohistochemical stains that may be useful in the differential diagnosis of metastatic colorectal tumors to the ovary and primary ovarian tumors are detailed.展开更多
AIM： To examine the expressions of N-cadherin and E-cadherin in specimens of 62 normal esophageal epithela, 31 adjacent atypical hyperplastic epithelia and 62 esophageal squamous cell carcinomas （ESCCs）, and to inv...AIM： To examine the expressions of N-cadherin and E-cadherin in specimens of 62 normal esophageal epithela, 31 adjacent atypical hyperplastic epithelia and 62 esophageal squamous cell carcinomas （ESCCs）, and to investigate the roles of N-cadherin in the invasiveness of ESCC cell line EC9706 transfected by N-cadherin shRNA.METHODS： PV immunohistochemistry was used to detect the expression pattern of N-cadherin and E-cadherin in specimens of 62 normal esophageal epithelia, 31 adjacent atypical hyperplastic epithelia and 62 ESCCs. The invasiveness of ESCC line EC9706 was determined by transwell assay after EC9706 was transfected by N-cadherin shRNA.RESULTS： The positive rotes of N-cadherin decreased in the carcinoma, adjacent atypical hyperplastic and normal esophageal tissues （75.8%, 61.3% and 29.0%, P 〈 0.05）, respectively, while those of E-cadherin increased （40.3%, 71.0% and 95.2%, P 〈 0.05）. The increased expression of N-cadherin and decreased expression of E-cadherin were related to invasion, differentiation, and lymph node metastasis （P 〈 0.05）. The expression level of N-cadherin decreased in the N-cadherin knocked down cells, and the invasiveness of those cells decreased significantly as well. The number of cells which crossed the basement membrane filter decreased from 123.40 ± 8.23 to 49.60 ±6.80 （P 〈 0.05）.CONCLUSION： E-cadherin and N-cadherin expression is correlated with the invasion and aggravation of ESCC. The down-regulation of N-cadherin lowers the invasiveness of EC9706 cell line.展开更多
Objective:The present study was designed to evaluate the effects of adjuvant chemotherapy(CT)vs.radiotherapy(RT,alone or combined with CT)on the prognosis of patients with high-risk,early-stage(stage I and stage II)en...Objective:The present study was designed to evaluate the effects of adjuvant chemotherapy(CT)vs.radiotherapy(RT,alone or combined with CT)on the prognosis of patients with high-risk,early-stage(stage I and stage II)endometrioid endometrial carcinoma.Methods:This single-center retrospective clinical study was conducted in Union Hospital,Tongji Medical College,Huazhong University of Science and Technology between 2010 and 2019.展开更多
BACKGROUND Ovarian endometrioid carcinoma resembling sex cord-stromal tumor(ECSCSs)is rare.CASE SUMMARY We present a rare case of primary ECSCSs in the left ovary.A 39-year-old female patient had persistent dull pain ...BACKGROUND Ovarian endometrioid carcinoma resembling sex cord-stromal tumor(ECSCSs)is rare.CASE SUMMARY We present a rare case of primary ECSCSs in the left ovary.A 39-year-old female patient had persistent dull pain in the lower abdomen for more than 1 mo,and she was initially diagnosed with pelvic inflammatory disease at a hospital.The patient received transabdominal hysterectomy,bilateral salpingo-oophorectomy,and pelvic and para-aortic lymph node dissection at our hospital and finally diagnosed with ECSCSs.After the operation,the patient received eight courses of cisplatinum+etoposide+bleomycin chemotherapy treatment and no evidence of tumor recurrence or metastasis was found in a 2-year follow-up period.CONCLUSION Ovarian endometrioid carcinoma is similar to the ovary sex cord-stromal tumor,especially when the cord-like structure is obvious.The clinical diagnosis for this tumor is difficult before surgery and pathology examination.The necessary immunohistochemical markers are of positive significance for assisting diagnosis and differential diagnosis.展开更多
Objectives. To determine overexpression of p53, EGFR, c-erbB-2 and c-erbB-3 in endometrioid carcinoma of the ovary and to evaluate the prognostic significance of these results, especially, coexisting overexpression of...Objectives. To determine overexpression of p53, EGFR, c-erbB-2 and c-erbB-3 in endometrioid carcinoma of the ovary and to evaluate the prognostic significance of these results, especially, coexisting overexpression of p53 and one of the member of type I growth factor receptor family.Methods. Overexpressions of the p53, EGFR, c-erbB-2 and c-erbB-3 protein were studied by im-munohistochemistry in paraffin-embedded tumor tissue from 28 patients with endometrioid carcinoma of the ovary.Results. 11 (39.3%), 13 (46. 4%), and 14 (50. 0%) were stained positively with p53, c-erbB-2 and c-erbB-3 monoclonal antibodies. 13 (46- 4%) was stained positively with EGFR polyclonal antibody. There were no relationship between p53, EGFR, C-erbB-2, c-erbB-3 and histologic grade, lymph node metastasis. The percentage of tumors with over expression of p53, EGFR, C-erbB-2 and c-erbB-3 was higher in those with stage Ⅱ-Ⅲ tumors compared with those with stage Ⅰ , in patients with residual tumor after initial surgery compared with those without. A high survival rate was observed in patients without p53, EGFR, c-erbB-2 and c-erbB-3 overexpression respectively than those with. A highest survival rate was observed in patients with both p53 and one of EGFR, c-erbB-2 and c-erbB-3 negative compared with those both positive or either of both positive.Conclusion. Overexpression of p53, EGFR, c-erbB-2 and C-erbB-3 resulted in a poorer prognosis respectively. Overexpression of both p53 and one of the EGFR, c-erbB-2 and c-erbB-3 is a worse prognostic indicator in patients with endometrioid carcinoma of the ovary.展开更多
目的探讨子宫低级别子宫内膜样癌患者淋巴脉管间隙浸润(lymphovascular space invasion,LVSI)的影响因素。方法回顾性分析2015年1月~2023年5月167例手术病理诊断低级别(G1、G2)子宫内膜样癌患者的临床资料。选择年龄、是否绝经、异常阴...目的探讨子宫低级别子宫内膜样癌患者淋巴脉管间隙浸润(lymphovascular space invasion,LVSI)的影响因素。方法回顾性分析2015年1月~2023年5月167例手术病理诊断低级别(G1、G2)子宫内膜样癌患者的临床资料。选择年龄、是否绝经、异常阴道出血时间、合并代谢综合征、CA125升高(≥35 U/ml)、子宫内膜厚度、宫腔占位、合并子宫腺肌症、国际妇产科联盟(International Federation of Gynecology and Obstetrics,FIGO)2009分期9项指标进行单因素分析,对P<0.05的因素进行二元logistic回归分析。结果167例子宫低级别子宫内膜样癌患者中,LVSI 24例(14.4%)。对单因素分析中P<0.05的4项因素(异常阴道出血时间≥4个月、CA125升高、合并子宫腺肌症、分期Ⅱ期及以上)进行二元logistic回归分析,结果显示分期Ⅱ期及以上(OR=7.357,95%CI:2.140~25.288,P=0.002),CA125升高(OR=4.883,95%CI:1.612~14.794,P=0.005)为子宫低级别子宫内膜样癌LVSI的独立预后因素。结论FIGO 2009分期Ⅱ期及以上、CA125≥35 U/ml与子宫低级别子宫内膜样癌患者LVSI有关,间接提示淋巴结转移风险,术前应高度关注,以便制定更为精准的手术方案。展开更多
目的探讨用重组苦瓜蛋白MAP30(Momordica anti-HIV protein of 30 ku)诱导人食管癌细胞株EC-1.71细胞凋亡状况。方法 MAP30诱导EC-1.71细胞后,透射电镜观察细胞凋亡的形态特征;JC-1荧光探针检测细胞线粒体膜电位(ΔΨm)变化;ELISA法检...目的探讨用重组苦瓜蛋白MAP30(Momordica anti-HIV protein of 30 ku)诱导人食管癌细胞株EC-1.71细胞凋亡状况。方法 MAP30诱导EC-1.71细胞后,透射电镜观察细胞凋亡的形态特征;JC-1荧光探针检测细胞线粒体膜电位(ΔΨm)变化;ELISA法检测细胞培养上清液中细胞色素c(Cytc)和肿瘤坏死因子-α(TNF-α)的水平;免疫细胞化学法检测细胞ERK1/PERK1,AKT/PAKT和NF-κB表达;荧光微板阅读仪检测细胞caspase-12活性。结果 MAP30诱导可以使EC-1.71细胞固缩,核膜扭曲,核染色质聚集成块并靠近核膜,内质网高度膨胀、扩张;ΔΨm水平下降;Cytc和TNF-α含量增加;胞质中ERK1、AKT表达增强而胞核中PERK1、PAKT表达降低,NF-κB活性降低,caspase-12活性增强。结论 MAP30可以诱导EC-1.71细胞凋亡。展开更多
文摘Metastasis of colorectal adenocarcinoma of the ovary is not an uncommon occurrence and ovarian metastases from colorectal carcinoma frequently mimic endometrioid and mucinous primary ovarian carcinoma.The clinical and pathologic features of metastatic colorectal adenocarcinoma involving the ovary is reviewed with particular focus on the diagnostic challenge of distinguishing these secondary ovarian tumors from primary ovarian neoplasm.Immunohistochemical stains that may be useful in the differential diagnosis of metastatic colorectal tumors to the ovary and primary ovarian tumors are detailed.
基金Supported by The National Natural Science Foundation of China,072102310054
文摘AIM： To examine the expressions of N-cadherin and E-cadherin in specimens of 62 normal esophageal epithela, 31 adjacent atypical hyperplastic epithelia and 62 esophageal squamous cell carcinomas （ESCCs）, and to investigate the roles of N-cadherin in the invasiveness of ESCC cell line EC9706 transfected by N-cadherin shRNA.METHODS： PV immunohistochemistry was used to detect the expression pattern of N-cadherin and E-cadherin in specimens of 62 normal esophageal epithelia, 31 adjacent atypical hyperplastic epithelia and 62 ESCCs. The invasiveness of ESCC line EC9706 was determined by transwell assay after EC9706 was transfected by N-cadherin shRNA.RESULTS： The positive rotes of N-cadherin decreased in the carcinoma, adjacent atypical hyperplastic and normal esophageal tissues （75.8%, 61.3% and 29.0%, P 〈 0.05）, respectively, while those of E-cadherin increased （40.3%, 71.0% and 95.2%, P 〈 0.05）. The increased expression of N-cadherin and decreased expression of E-cadherin were related to invasion, differentiation, and lymph node metastasis （P 〈 0.05）. The expression level of N-cadherin decreased in the N-cadherin knocked down cells, and the invasiveness of those cells decreased significantly as well. The number of cells which crossed the basement membrane filter decreased from 123.40 ± 8.23 to 49.60 ±6.80 （P 〈 0.05）.CONCLUSION： E-cadherin and N-cadherin expression is correlated with the invasion and aggravation of ESCC. The down-regulation of N-cadherin lowers the invasiveness of EC9706 cell line.
文摘Objective:The present study was designed to evaluate the effects of adjuvant chemotherapy(CT)vs.radiotherapy(RT,alone or combined with CT)on the prognosis of patients with high-risk,early-stage(stage I and stage II)endometrioid endometrial carcinoma.Methods:This single-center retrospective clinical study was conducted in Union Hospital,Tongji Medical College,Huazhong University of Science and Technology between 2010 and 2019.
文摘BACKGROUND Ovarian endometrioid carcinoma resembling sex cord-stromal tumor(ECSCSs)is rare.CASE SUMMARY We present a rare case of primary ECSCSs in the left ovary.A 39-year-old female patient had persistent dull pain in the lower abdomen for more than 1 mo,and she was initially diagnosed with pelvic inflammatory disease at a hospital.The patient received transabdominal hysterectomy,bilateral salpingo-oophorectomy,and pelvic and para-aortic lymph node dissection at our hospital and finally diagnosed with ECSCSs.After the operation,the patient received eight courses of cisplatinum+etoposide+bleomycin chemotherapy treatment and no evidence of tumor recurrence or metastasis was found in a 2-year follow-up period.CONCLUSION Ovarian endometrioid carcinoma is similar to the ovary sex cord-stromal tumor,especially when the cord-like structure is obvious.The clinical diagnosis for this tumor is difficult before surgery and pathology examination.The necessary immunohistochemical markers are of positive significance for assisting diagnosis and differential diagnosis.
文摘Objectives. To determine overexpression of p53, EGFR, c-erbB-2 and c-erbB-3 in endometrioid carcinoma of the ovary and to evaluate the prognostic significance of these results, especially, coexisting overexpression of p53 and one of the member of type I growth factor receptor family.Methods. Overexpressions of the p53, EGFR, c-erbB-2 and c-erbB-3 protein were studied by im-munohistochemistry in paraffin-embedded tumor tissue from 28 patients with endometrioid carcinoma of the ovary.Results. 11 (39.3%), 13 (46. 4%), and 14 (50. 0%) were stained positively with p53, c-erbB-2 and c-erbB-3 monoclonal antibodies. 13 (46- 4%) was stained positively with EGFR polyclonal antibody. There were no relationship between p53, EGFR, C-erbB-2, c-erbB-3 and histologic grade, lymph node metastasis. The percentage of tumors with over expression of p53, EGFR, C-erbB-2 and c-erbB-3 was higher in those with stage Ⅱ-Ⅲ tumors compared with those with stage Ⅰ , in patients with residual tumor after initial surgery compared with those without. A high survival rate was observed in patients without p53, EGFR, c-erbB-2 and c-erbB-3 overexpression respectively than those with. A highest survival rate was observed in patients with both p53 and one of EGFR, c-erbB-2 and c-erbB-3 negative compared with those both positive or either of both positive.Conclusion. Overexpression of p53, EGFR, c-erbB-2 and C-erbB-3 resulted in a poorer prognosis respectively. Overexpression of both p53 and one of the EGFR, c-erbB-2 and c-erbB-3 is a worse prognostic indicator in patients with endometrioid carcinoma of the ovary.
文摘目的探讨子宫低级别子宫内膜样癌患者淋巴脉管间隙浸润(lymphovascular space invasion,LVSI)的影响因素。方法回顾性分析2015年1月~2023年5月167例手术病理诊断低级别(G1、G2)子宫内膜样癌患者的临床资料。选择年龄、是否绝经、异常阴道出血时间、合并代谢综合征、CA125升高(≥35 U/ml)、子宫内膜厚度、宫腔占位、合并子宫腺肌症、国际妇产科联盟(International Federation of Gynecology and Obstetrics,FIGO)2009分期9项指标进行单因素分析,对P<0.05的因素进行二元logistic回归分析。结果167例子宫低级别子宫内膜样癌患者中,LVSI 24例(14.4%)。对单因素分析中P<0.05的4项因素(异常阴道出血时间≥4个月、CA125升高、合并子宫腺肌症、分期Ⅱ期及以上)进行二元logistic回归分析,结果显示分期Ⅱ期及以上(OR=7.357,95%CI:2.140~25.288,P=0.002),CA125升高(OR=4.883,95%CI:1.612~14.794,P=0.005)为子宫低级别子宫内膜样癌LVSI的独立预后因素。结论FIGO 2009分期Ⅱ期及以上、CA125≥35 U/ml与子宫低级别子宫内膜样癌患者LVSI有关,间接提示淋巴结转移风险,术前应高度关注,以便制定更为精准的手术方案。
文摘目的探讨用重组苦瓜蛋白MAP30(Momordica anti-HIV protein of 30 ku)诱导人食管癌细胞株EC-1.71细胞凋亡状况。方法 MAP30诱导EC-1.71细胞后,透射电镜观察细胞凋亡的形态特征;JC-1荧光探针检测细胞线粒体膜电位(ΔΨm)变化;ELISA法检测细胞培养上清液中细胞色素c(Cytc)和肿瘤坏死因子-α(TNF-α)的水平;免疫细胞化学法检测细胞ERK1/PERK1,AKT/PAKT和NF-κB表达;荧光微板阅读仪检测细胞caspase-12活性。结果 MAP30诱导可以使EC-1.71细胞固缩,核膜扭曲,核染色质聚集成块并靠近核膜,内质网高度膨胀、扩张;ΔΨm水平下降;Cytc和TNF-α含量增加;胞质中ERK1、AKT表达增强而胞核中PERK1、PAKT表达降低,NF-κB活性降低,caspase-12活性增强。结论 MAP30可以诱导EC-1.71细胞凋亡。
