不同EMS处理时间对大豆诱变后代主要品质含量变化影响分析

为探究不同EMS处理时间对大豆诱变后代主要品质含量变化的影响,文章利用0.5%EMS溶液处理大豆高蛋白品系公交1057-1,分别浸泡4、7和10 h(清水冲洗处理为对照),对播种后获得的连续4代诱变后代主要品质含量变化进行分析。结果表明,与对照相较,经EMS处理后的植株成活率随处理时间延长而降低。其中,经EMS-4处理后的植株成活率最高,为43.5%;经EMS-10处理后的植株成活率最低,仅为16.0%。不同EMS处理时间对大豆诱变后代的蛋白质含量影响显著,对脂肪含量影响不明显。其中经EMS-4处理的诱变后代蛋白质含量高于其他3组处理;经EMS-4处理后的M2代蛋白质含量发生显著分离,并出现超亲个体,脂肪含量变化不显著。

达乌里胡枝子愈伤组织及种子EMS诱变研究

为优化达乌里胡枝子愈伤诱导培养基的激素含量及比例,明确甲基磺酸乙酯(EMS)诱变愈伤组织及种子的最佳条件,本研究以达乌里胡枝子种子为外植体,探究了不同激素配比对愈伤诱导的影响,并针对愈伤组织和种子处理分别设置了两时间两浓度(2 h和6 h,0.3%和0.9%)及三时间两浓度(6 h、8 h、10 h,0.4%、0.8%)的EMS处理时间和浓度的组合,研究各处理对愈伤分化系数和后期植株成活率以及种子发芽率、发芽势和幼苗表型变异的影响。结果表明,达乌里胡枝子种子愈伤的最佳激素配比为0.25 mg/L NAA+0.5 mg/L 6-BA+0.5 mg/L 2,4-D,此时达乌里胡枝子愈伤诱导率为94.9%。EMS诱变达乌里胡枝子愈伤组织的适宜浓度为0.3%,处理时间为6 h,分化系数和植株相对成活率分别为46.31%和47.78%,接近半致死范围,且生根数、根长和平均株高等指标与高浓度EMS处理相比受到的负面影响较小。EMS诱变达乌里胡枝子种子的适宜浓度为0.8%,处理时间10 h,发芽率、出苗率、成苗率分别为53.61%、51.06%和53.28%,均接近半致死量,同时发现多株幼苗叶片出现了明显的形态变异。本试验在达乌里胡枝子原有种质资源基础上,丰富了达乌里胡枝子突变体库,也为后续开展达乌里胡枝子诱变育种研究提供理论依据。

基于EMS管理模式的延续性护理在学龄前喘息性疾病儿童中的应用观察

目的·探讨基于EMS[环境管理(environment management,E)、用药指导(medicine direction,M)与自我监测(self monitoring,S)]管理模式的延续性护理在学龄前喘息性疾病儿童中的应用效果。方法·选取2019年12月至2020年11月,在上海交通大学医学院附属儿童医院呼吸科收治的67例0~6岁喘息性疾病患儿,按照随机数字表分为观察组33例和对照组34例,其中失访3例,最终每组32例。观察组采用基于EMS管理模式的延续性护理,对照组给予常规护理和出院电话随访。2组患儿出院后1、3、6个月随访评估儿童呼吸和哮喘测试(Test for Respiratory and Asthma Control in Kids,TRACK)结果、喘息复发情况;出院后6个月随访采用支气管哮喘用药依从性评分表(Medication Adherence Report Scale for Asthma,MARS-A)和护理工作满意度调查表评估用药依从性及护理工作满意度。结果·2组患儿人口学特征及临床基线特征差异无统计学意义。重复测量方差分析结果显示,时间、组别、组别×时间的交互作用对TRACK总分的影响均有统计学意义;出院后1、3、6个月,观察组TRACK总分均显著高于对照组(均P=0.000);2组患儿TRACK总分均随时间推移逐渐上升(P=0.000)。观察组1、3、6个月随访发现喘息复发率分别为25.0%、18.7%、9.4%,均显著低于对照组(分别为50.0%、43.7%、31.3%,均P<0.05);广义估计方程分析显示组间比较差异有统计学意义(P=0.013),观察组干预效果优于对照组(OR=0.292)。出院后6个月观察组MARS-A得分为(4.519±0.395)分,显著高于对照组[(3.994±0.739)分,P=0.001]。护理工作满意度调查结果显示,观察组显著高于对照组(P=0.000)。患儿MARS-A得分与护理工作满意度呈中度正相关(r=0.389,P=0.001)。结论·基于EMS管理模式的延续性护理可显著提高学龄前喘息性疾病儿童的用药依从性和喘息控制水平,明显降低喘息复发率,以及提高护理工作满意度。

EMS诱变菜豆子叶节定向筛选耐羟脯氨酸(HYP)突变体

以双丰一号菜豆子叶节为外植体,利用化学诱变剂EMS处理引发基因突变,进行突变体定向筛选抗逆突变细胞系。以0.17%EMS诱变处理2 h,子叶节的不再生率接近50%;以羟脯氨酸最大致死量3.5 mmol/L为筛选压力,定向筛选抗逆突变体。结果显示,在羟脯氨酸筛选培养基上,经EMS诱变的子叶节存活31个,突变率为8.61%,存活的子叶节再生率为74.19%,成功获得耐羟脯氨酸的突变株系。

上海某院EMS转运急性缺血性脑卒中患者院前延误的回顾性研究

目的 探究启动EMS(emergency medical service,紧急医疗服务)转运的急性缺血性脑卒中患者院前延误的影响因素,为急性缺血性脑卒中患者减少延误提高策略。方法 对132例启动EMS的急性缺血性脑卒中患者进行相关影响因素分析,包括年龄、性别、呼救时天气情况、脑卒中知识认知度,首发症状等,分为延误组(n=29)和及时组(n=103),通过多因素Logistics回归分析确定造成院前延误的影响因素。结果 两组患者在不同天气情况、卒中知识认知度、首发症状上存在差异(P<0.05)。多因素Logistics回归分析显示天气情况(OR=0.581,95%CI:0.358~0.943),卒中知识认知度(OR=0.238,95%CI:0.098~0.578)及首发症状为意识障碍(OR=0.076,95%CI:0.008~0.717)是减少院前延误的独立影响因素(P<0.05)。结论 较高卒中知识认知度、首发症状有意识障碍、天气情况良好有利于减少启动EMS转运的急性缺血性脑卒中患者的院前延误。因此,加强公众卒中知识普及培训,提高公众卒中意识,尽快启动EMS系统,可减少院前延误,改善患者预后。

保护装置交流回路EMS测试对源影响的研究

在继电保护电磁兼容标准中,对于保护装置交流回路端口有严格的电磁兼容抗扰度试验要求。作为一种辅助设备或回路——给保护装置交流采样回路加激励量的“源”,必须保持“干净”,这是抗干扰性能测试的重要影响因素。“源”干净与否,会对测试结果本身产生重要影响。本文旨在分析这些影响原因,并对如何消除影响提出了一些建议。

Distinct molecular targets of ProEGCG from EGCG and superior inhibition of angiogenesis signaling pathways for treatment of endometriosis

Endometriosis is a common chronic gynecological disease with endometrial cell implantation outside the uterus.Angiogenesis is a major pathophysiology in endometriosis.Our previous studies have demonstrated that the prodrug of epigallocatechin gallate(ProEGCG)exhibits superior anti-endometriotic and anti-angiogenic effects compared to epigallocatechin gallate(EGCG).However,their direct binding targets and underlying mechanisms for the differential effects remain unknown.In this study,we demonstrated that oral ProEGCG can be effective in preventing and treating endometriosis.Additionally,1D and 2D Proteome Integral Solubility Alteration assay-based chemical proteomics identified metadherin(MTDH)and PX domain containing serine/threonine kinase-like(PXK)as novel binding targets of EGCG and ProEGCG,respectively.Computational simulation and BioLayer interferometry were used to confirm their binding affinity.Our results showed that MTDH-EGCG inhibited protein kinase B(Akt)-mediated angiogenesis,while PXK-ProEGCG inhibited epidermal growth factor(EGF)-mediated angiogenesis via the EGF/hypoxia-inducible factor(HIF-1a)/vascular endothelial growth factor(VEGF)pathway.In vitro and in vivo knockdown assays and microvascular network imaging further confirmed the involvement of these signaling pathways.Moreover,our study demonstrated that ProEGCG has superior therapeutic effects than EGCG by targeting distinct signal transduction pathways and may act as a novel antiangiogenic therapy for endometriosis.

Endometriosis beyond the Pelvis: A Case Series of Cutaneous Endometriosis and Literature Review

Introduction: Cutaneous endometriosis is an uncommon but well-known skin disorder that represents about 0.5% to 1% of all endometriosis. The objective of this case series is to report clinical presentation, diagnosis, and management of various forms of cutaneous endometriosis. Material and Methods: It was an observational, retrospective and descriptive review of cases presenting with cutaneous endometriosis among Cameroonian women managed at the gynaecological outpatient department of Yaounde Gynaeco-Obstetric and Pediatric Hospital. All the following parameters were analysed: age, parity, previous pelvic surgery, presenting symptoms and duration, associated symptoms, localizations, imaging, size of the lesion, other localization of endometriosis, management and histopathological results. Results: we reported 4 cases of cutaneous endometriosis, with 3 umbilical endometriosis and 1 abdominal scar endometriosis. Patient age ranged from 28 to 39 years with an average of 33 years. All patients described infertility (two primary and two secondary) and two had a history of abdominal surgery. All patients presented local cyclical signs such as pain, swelling, color change and bleeding. The duration of symptoms varied from 2 to 3 years and the size of lesions ranged from 2 to 3.5 cm for umbilical lesions and was 9 cm for abdominal scar endometriosis. In all cases, no imaging was required for the diagnosis, which was suspected on the basis of patient’s history and the cyclical nature of local signs, followed by wide surgical excision and confirmation on histopathology. Conclusion: Cutaneaous endometriosis is a rare benign condition. Umbilical endometriosis seems to be the main cutaneous localization and can be described as primary or secondary. Even if its diagnosis must be confirmed by histopathology, it should be considered in patient with cutaneous cyclic signs such as pain, swelling or bleeding with or without history of abdominal surgery.

Can Tibetan medicine Honghua Ruyi pills relieve endometriosisassociated dysmenorrhea?Protocol for a randomized placebocontrolled trial

Objective:To provide high-quality clinical evidence of the efficacy of Tibetan medicine Honghua Ruyi(HHRY)pills for endometriosis-associated dysmenorrhea.Methods:This study constitutes a multicenter,randomized,double-blind,placebo-controlled trial encompassing a three-menstrual cycle intervention followed by a three-menstrual cycle follow-up period.A total of 164 eligible females with endometriosis-associated dysmenorrhea were randomly divided into HHRY pills and placebo groups in a 1:1 ratio.The primary outcome included dysmenorrhea symptoms assessed using Visual Analog Scale(VAS)scores and quality of life,whereas the secondary outcome measures included the maximum VAS for non-menstrual pelvic pain,duration of pain episodes(in days),frequency and quantity of the consumption of ibuprofen sustained-release capsules(or other non-steroidal anti-inflammatory drugs),and days off work/study for staff/student due to dysmenorrhea,ovarian cyst,and/or pelvic nodule size.The safety was monitored throughout the treatment period.All the analyses were based on the intention-to-treat principle.For continuous outcomes,simple or multiple linear regressions were used to estimate the differences between the HHRY pills and placebo groups,with categorical data expressed as the number and percentage of occurrences.Differences were compared using the chi-square test or Fisher's exact test.The predefined analysis was adjusted for concomitant treatment,a variable considered to be associated with outcomes but unaffected by treatment allocation.Estimates of treatment effects were reported with 95%confidence intervals.Two-tailed P values≤.05 were considered statistically significant.Conclusion:Positive results from this trial,upon completion would provide robust evidence for the efficacy and safety of HHRY pills in treating dysmenorrhea in patients with endometriosis.

Immune pathway through endometriosis to ovarian cancer

Endometriosis is an estrogen-dependent inflammatory disease,defined by the presence of functional endometrial tissue outside of the uterine cavity.This disease is one of the main gynecological diseases,affecting around 10%-15%women and girls of reproductive age,being a common gynecologic disorder.Although endometriosis is a benign disease,it shares several characteristics with invasive cancer.Studies support that it has been linked with an increased chance of developing endometrial ovarian cancer,representing an earlier stage of neoplastic processes.This is particularly true for women with clear cell carcinoma,low-grade serous carcinoma and endometrioid.However,the carcinogenic pathways between both pathologies remain poorly understood.Current studies suggest a connection between endometriosis and endometriosis-associated ovarian cancers(EAOCs)via pathways associated with oxidative stress,inflammation,and hyperestrogenism.This article aims to review current data on the molecular events linked to the development of EAOCs from endometriosis,specifically focusing on the complex relationship between the immune response to endometriosis and cancer,including the molecular mechanisms and their ramifications.Examining recent developments in immunotherapy and their potential to boost the effectiveness of future treatments.

Hupo powder promotes autophagy of menstrual blood-derived stem cells from patients with endometriosis

Objective:To explore the effect of Hupo powder(HP)on autophagy in menstrual blood-derived stem cells(MenSCs)with endometriosis(EMT).Methods:EMT MenSCs(E-MenSCs)and healthy MenSCs(H-MenSCs)were isolated from the menstrual blood of patients with EMT and healthy female participants,respectively.We identified their stem cells’characteristics via adipogenic and osteogenic differentiation.Twelve male SpragueeDawley rats received 0.9% NaCl and HP-dispensing granules by gastric irrigation to prepare blank serum and medicated serum,respectively.We used serum concentrations of 5%,10%,and 20%,each at administered times of 12,24,and 48 h to select the best condition.These cells were divided into three groups:blank serum of the control group,blank serum of the model group,and medicated serum of the HP group.H-MenSCs were used in the control group,while E-MenSCs were used in the model and HP groups.We analyzed cell viability using a cell counting kit-8 assay,observed cell morphology,evaluated the amounts of auto-phagosomes and autolysosomes by transmission electron microscopy,and detected the protein expression of autophagy markers(LC3-II and Beclin1)by Western blot.Results:E-MenSCs and H-MenSCs became long fusiform with a diffuse radial pattern,forming lipid droplets and calcium nodules after adipogenic and osteogenic differentiation.We then used the best conditiond 20% serum and 48 hdfor the subsequent experiments.In contrast to the model group,the HP group exhibited lower cell viability(=0.007),larger amounts of autophagosomes and autolysosomes(P<0.001 and P=0.001,respectively),and higher expression of LC3-II and Beclin1(P=0.021 and P=0.019,respectively).Conclusion:Hupo powder can promote autophagy in E-MenSCs,which might be one of the mechanisms underlying its therapeutic effects.

Modeling the Physical Parameters of Solenoids for MEMS Applications

The paper is devoted to study of the electrical parameters of the motion parts of the MEMS such as solenoids. The analytical background is given in order to describe the influence of the electrical field components on the forces, which are result of interaction of the electromagnetic (EM) field components with the parts of motion devices of MEMS. The given analytical formulas open the ability to calculate the self-inductance of the microsolenoids of the different kind, as well as the stored energy of such motion devices, that could be used for the modeling and optimization of parameters of running devices of MEMS such as actuators, sensors etc.

监控画面信号自动巡视技术在电化学储能EMS中的应用

电化学储能电站普遍采用组态型EMS,通过查看监控画面实现对储能电站电池、BMS、PCS、消防等设备运行状态的监视。提出一种在锂离子电化学储能电站EMS系统中自动巡视监控画面数据的方法,通过周期性自动扫描监控画面上的设备运行数据,识别信号点的数据品质类型,将设备的实时状态数据与健康特征值做对比,主动发现画面上遥测越限、数据不刷新、封锁、遥测遥信不匹配等异常数据,识别异常设备,生成异常数据报告。通过采用这种画面自动巡视技术,可以减轻储能电站监控员的画面监盘工作压力,提高储能电站的运行效率和安全性。

Difficulties Faced by Physicians in the Diagnosis of Endometriosis in the Brazilian Public Health System

Endometriosis is a clinical and recurrent condition characterized by the presence of functional endometrial tissue outside the uterine cavity and myometrium. It is estimated that 6% to 10% of women of reproductive age, 50% to 60% of adolescents and adults with pelvic pain, and up to 50% of women with infertility are pregnant from the condition. However, this proportion tends to be even greater due to the difficulty in diagnosing it: because it presents different symptoms, lack of information from health professionals, and a high-cost diagnostic test. Another aggravating factor is the average time from the onset of symptoms to the medical diagnosis, which even today ranges from five to ten years;this delay has consequences for disease progression, as it prevents early treatment, which is important for improving pain levels and physical and psychological well-being. Aware of these mishaps, it is therefore important to assess the difficulties faced by doctors in the diagnosis of endometriosis in the public health system, in Brazil, the Unified Health System (SUS). With this purpose, a cross sectional was carried out, developed in the virtual environment using traffic in Google forms, with the difficulties quantified in the Likert scale. Participating physicians pointed out a medium difficulty for patients with endometriosis to access the consultation (58%) and moderate difficulty (52%) to perform an ultrasound examination;also signaled great difficulty in performing magnetic resonance imaging (77%) and laparotomy (61%) in these patients. It is hoped that knowledge about the difficulties faced by physicians in the diagnosis of endometriosis in the SUS will give these physicians a voice and corroborate, even if minimally, so that there is more research that provokes the search for these difficulties, in order to improving the quality of life of these women.

腹腔镜联合亮丙瑞林治疗EMS伴不孕疗效及对血清性激素影响

目的:观察腹腔镜联合亮丙瑞林治疗子宫内膜异位症(EMS)伴不孕疗效及对血清雌二醇(E_(2))、卵泡刺激素(FSH)和抗缪勒管激素(AMH)的影响。方法:选取2019年4月-2022年4月本院接受治疗的EMS伴不孕患者103例,以接受腹腔镜治疗为对照组(52例),接受腹腔镜联合亮丙瑞林治疗为联合组(51例)。比较两组手术后治疗总有效率,手术前后血清FSH、E_(2)、AMH水平,血清肿瘤标志物糖链抗原125(CA125)、糖链抗原199(CA199)水平;两组并发症及术后1年妊娠率、复发率。结果:术后3个月,联合组总有效率(90.2%)高于对照组(73.1%);术后6个月联合组血清FSH、E_(2)水平均低于对照组(P<0.05),两组AMH无差异(P>0.05),联合组血清CA125(21.64±4.36 U/L)、CA199(24.36±2.24 U/L)水平均低于对照组(33.70±6.92 U/L、35.48±3.22 U/L)(P<0.05)。术后1年联合组妊娠率(86.3%)高于对照组(44.2%),复发率(7.8%)低于对照组(23.1%)(均P<0.05)。两组治疗期间并发症发生率(9.8%、5.8%)无差异(P>0.05)。结论:腹腔镜辅助亮丙瑞林治疗EMS伴不孕能够显著提高治疗效果,改善血清性激素及肿瘤标志物水平,提高妊娠率,减少复发,且安全性较好。

Identification of EML4 as a key hub gene for endometriosis and its molecular mechanism and potential drug prediction based on the GEO database

Objective:Key genes were screened to analyze molecular mechanisms and their drug targets of endometriosis by applying a bioinformatics approach.Methods:Gene expression profiles of endometriosis and healthy controls were obtained from the Gene Expression Omnibus database.Significant differentially expressed genes were screened using the limma package.Correlation pathways were screened by Spearman correlation analysis on the echinoderm microtubule-associated protein-like 4(EML4)and enrichment in endometriosis pathways and estimated by the GSVA package.Immune characteristics were assessed by the“ESTIMATE”R package.Potential regulatory pathways were determined by enrichment analysis.The SWISS-MODE website was used in homology modeling with EML4 and EML4 protein activity was predicted.VarElect was employed in molecular docking for screening potential compound inhibitors targeting endometriosis.Results:Ten endometriosis and 10 normal samples were included.EML4 was significantly upregulated in endometriosis(p<0.05).Thirty significantly correlated pathways involving 18 positive and 12 negative correlations,including GLYCOSAMINOGLYCAN_BIOSYNTHESIS_HEPARAN_SULFATE and GLYCOSPHINGOLIPID_BIOSYNTHESIS_GANGLIO_SERIES were screened between EML4 and endometriosis.Immunocorrelation analysis showed a significant difference in immune-related pathways in endometriosis and normal samples(p<0.05).In endometriosis,EML4 was associated with T-cell CD4 resting memory,activated mast cells,plasma cells,activated NK cells,M2 macrophages,and follicular helper T cells(p<0.05).Molecular docking identified five potential inhibitors of EML4,and compound DB05104(asimadoline)bound well to EML4 protein to exert its physiological effects.Conclusion:Differential gene expression and immune correlation analyses revealed that EML4 may affect endometriosis through multiple targets and pathways,the mechanism of which involved immune cell activation and infiltration.Molecular docking and dynamics simulation verified DB05104 as a potential inhibitor of EML4 and a powerful target for endometriosis treatment.

Epidemiological Profile and Location of Lesions According to #ENZIAN of Patients Operated on for Endometriosis in a Public Endoscopic Surgery Centre, Yaounde, Cameroon

Introduction: Endometriosis is a chronic disease affecting approximately 10% of women. Our aim was to describe the epidemiological profile, clinical presentation and location of lesions in patients operated on for endometriosis. Methodology: We conducted an analytical cross-sectional study with retrospective data collection over a period of 05 years from 1 January 2017 to 31 March 2022. Our study population consisted of women who had undergone laparoscopy for endometriosis. Data analysis was performed using SPSS software version 23.0. Results: In total we included 159 patients who had undergone laparoscopy for endometriosis. The mean age was 32.9 ± 5.9 years, most of whom were nuligravida 61.6% (n = 99). Infertility was the most frequent reason for consultation, accounting for 72.3% (n = 115), followed by dysmenorrhoea in 60.8% (n = 96) of cases and chronic pelvic pain in 37.7% (n = 60). In addition, 46 (28.9%) patients complained of deep dyspareunia, 17 (10.7%) of dyschezia and nine (5.7%) of dysuria. Magnetic Resonance Imaging (MRI) was used in one patient. Diagnostic laparoscopy was performed directly in 77 (48.4%) patients. The lesions were multifocal, according to the #ENZIAN classification nomenclature. Tubal lesions were most frequently found 107 (67.3%) on the left, followed by compartment B (uterosacral ligaments, cardinal ligaments and lateral pelvic wall) 49.7% (n = 79), and endometriomas 40.9% (n = 65). Conclusion: Women operated on for endometriosis were of a more advanced age, and predominantly nulliparous. Infertility was the most frequent reason for consultation and tubal location most frequently encountered for superficial lesions and compartiment B for deep lesions according to #ENZIA.

EMS高级应用软件和DTS培训系统在电网运行与调度员培训中的应用

本文主要介绍EMS高级应用软件和DTS培训系统在电网运行与调度员培训中的应用策略。EMS高级应用软件在电网运行中发挥重要作用,具有监测、控制、优化和安全管理等功能。DTS培训系统则满足调度员培训的需求,通过模拟和实践训练提升其应急处理和团队协作能力。在电网运行与调度员培训的应用策略中,安全管理、运行优化、紧急事件处理和团队协作是关键方面。

EMS在海上油气田电网的升级应用

与陆地电网不同,海上油气田电网总装机容量小、可调控负载裕量有限,因此对稳定性要求特别高。当电网出现类似发电机跳机、电网解列、线路过载或故障、变压器过载等影响电网稳定的因素时,需要一种稳定的控制策略,来调控电网的发电机出力与负荷相平衡。某海上油气田通过电网改造,实现多电站并网运行,构建的大电网既扩大了整个电网容量,又提高富余电力的利用率。同时,电网基于原有PMS的硬件基础,通过工控网络重构与扩容、PLC点数扩容、Case策略重规划的方式,升级为EMS。应用了EMS进行管控的电网,具有多电站并网运行、电力统一智能、抗干扰能力强和后期风力发电机并网扩容简易等诸多优点。

电网EMS中区域备自投控制技术运用研究

本文结合实际,阐述区域备自投控制技术在能源管理系统中的实际应用。该技术具备快速恢复供电、自动化操作、运行可靠的优势,在配电网中和输电网中被广泛应用。研究表明,区域备自投控制技术应用需建立系统模型,采用相应的算法加以控制优化,并在安装完成后测试,以确保其应用效果良好。