Dose-response effect of pre-exercise carbohydrates under muscle glycogen unavailability:Insights from McArdle disease

Background:This study aimed to determine the effect of different carbohydrate(CHO)doses on exercise capacity in patients with McArdle disease—the paradigm of“exercise intolerance”,characterized by complete muscle glycogen unavailability—and to determine whether higher exogenous glucose levels affect metabolic responses at the McArdle muscle cell(in vitro)level.Methods:Patients with McArdle disease(n=8)and healthy controls(n=9)underwent a 12-min submaximal cycling constant-load bout followed by a maximal ramp test 15 min after ingesting a non-caloric placebo.In a randomized,double-blinded,cross-over design,patients repeated the tests after consuming either 75 g or 150 g of CHO(glucose:fructose=2:1).Cardiorespiratory,biochemical,perceptual,and electromyographic(EMG)variables were assessed.Additionally,glucose uptake and lactate appearance were studied in vitro in wild-type and McArdle mouse myotubes cultured with increasing glucose concentrations(0.35,1.00,4.50,and 10.00 g/L).Results:Compared with controls,patients showed the“classical”second-wind phenomenon(after prior disproportionate tachycardia,myalgia,and excess electromyographic activity during submaximal exercise,all p<0.05)and an impaired endurance exercise capacity(-51%ventilatory threshold and55%peak power output,both p<0.001).Regardless of the CHO dose(p<0.05 for both doses compared with the placebo),CHO intake increased blood glucose and lactate levels,decreased fat oxidation rates,and attenuated the second wind in the patients.However,only the higher dose increased ventilatory threshold(+27%,p=0.010)and peak power output(+18%,p=0.007).In vitro analyses revealed no differences in lactate levels across glucose concentrations in wild-type myotubes,whereas a doseresponse effect was observed in McArdle myotubes.Conclusion:CHO intake exerts beneficial effects on exercise capacity in McArdle disease,a condition associated with total muscle glycogen unavailability.Some of these benefits are dose dependent.

Does ergogenic effect of caffeine supplementation depend on CYP1A2 genotypes? A systematic review with meta-analysis

Background:The ergogenic effects of caffeine intake on exercise performance are well-established,even if differences exist among individuals in response to caffeine intake.The genetic variation of a specific gene,human cytochrome P450 enzyme 1A2(CYP1A2)(rs762551),may be one reason for this difference.This systematic review and meta-analysis aimed to comprehensively evaluate the influence of CYP1A2 gene types on athletes’exercise performance after caffeine intake.Methods:A literature search through 4 databases(Web of Science,PubMed,Scopus,and China National Knowledge Infrastructure)was conducted until March 2023.The effect size was expressed as the weighted mean difference(WMD)by calculating fixed effects meta-analysis if heterogeneity was not significant(I^(2)≤50%and p≥0.1).Subgroup analyses were performed based on AA and AC/CC genotype of CYP1A2.Results:The final number of studies meeting the inclusion criteria was 12(n=666 participants).The overall analysis showed that the cycling time trial significantly improved after caffeine intake(WMD=-0.48,95%confidence interval(95%CI):-0.83 to-0.13,p=0.007).In subgroup analyses,acute caffeine intake improved cycling time trial only in individuals with the A allele(WMD=-0.90,95%CI:-1.48 to-0.33,p=0.002),but not the C allele(WMD=-0.08,95%CI:-0.32 to 0.17,p=0.53).Caffeine supplementation did not influence the Wingate(WMD=8.07,95%CI:-22.04 to 38.18,p=0.60)or countermovement jump test(CMJ)performance(WMD=1.17,95%CI:-0.02 to 2.36,p=0.05),and these outcomes were not influenced by CYP1A2 genotype.Conclusion:Participants with the CYP1A2 genotype with A allele improved their cycling time trials after caffeine supplementation.However,compared to placebo,acute caffeine supplementation failed to increase the Wingate or CMJ performance,regardless of CYP1A2 genotype.

Optimized operation scheme of flash-memory-based neural network online training with ultra-high endurance

With the rapid development of machine learning,the demand for high-efficient computing becomes more and more urgent.To break the bottleneck of the traditional Von Neumann architecture,computing-in-memory(CIM)has attracted increasing attention in recent years.In this work,to provide a feasible CIM solution for the large-scale neural networks(NN)requiring continuous weight updating in online training,a flash-based computing-in-memory with high endurance(10^(9) cycles)and ultrafast programming speed is investigated.On the one hand,the proposed programming scheme of channel hot electron injection(CHEI)and hot hole injection(HHI)demonstrate high linearity,symmetric potentiation,and a depression process,which help to improve the training speed and accuracy.On the other hand,the low-damage programming scheme and memory window(MW)optimizations can suppress cell degradation effectively with improved computing accuracy.Even after 109 cycles,the leakage current(I_(off))of cells remains sub-10pA,ensuring the large-scale computing ability of memory.Further characterizations are done on read disturb to demonstrate its robust reliabilities.By processing CIFAR-10 tasks,it is evident that~90%accuracy can be achieved after 109 cycles in both ResNet50 and VGG16 NN.Our results suggest that flash-based CIM has great potential to overcome the limitations of traditional Von Neumann architectures and enable high-performance NN online training,which pave the way for further development of artificial intelligence(AI)accelerators.

From Trends to Drivers:Key Factors Propelling Electric Vehicle Sales

With the rapid advancement of human economic levels and modern civilization,the automobile manufacturing industry is increasingly confronted with challenges related to energy scarcity and environmental pollution.Low carbon emissions and energy savings have become the main focus of automotive development.Under the influence of government incentives,the sales of household electric vehicles(EVs)have increased significantly,although they still represent a small share of the overall car market.To examine the factors influencing consumer purchases of household EVs,this report integrates both qualitative and quantitative analyses,controlling for single variables.Using linear regression,an empirical analysis was conducted on 18 BYD models with varying ranges and prices.The results indicate a strong positive correlation between driving range,selling price,and EV sales.Looking ahead,the development of new energy vehicles should prioritize longer ranges,high-quality features,and cost-effective performance.

Effect of N-Acetylcysteine Combined with Lung Rehabilitation Therapy on Exercise Endurance and Quality of Life of Patients with Rheumatoid Arthritis-Related Interstitial Lung Disease

Objective:To explore the effect of N-acetylcysteine combined with lung rehabilitation therapy on exercise endurance and quality of life in patients with rheumatoid arthritis-related interstitial lung disease(RA-ILD).Methods:Fifty-six patients with RA-ILD admitted to Xijing Hospital from May 2022 to January 2024 were randomly divided into two groups:a non-rehabilitation group and a pulmonary rehabilitation group,with 28 patients in each group.Both groups received routine treatment.Additionally,the non-rehabilitation group received N-acetylcysteine treatment,while the lung rehabilitation group received lung rehabilitation treatment in addition to N-acetylcysteine.The improvement in exercise endurance and dyspnea between the two groups after treatment was compared and the quality of life of the patients was observed.Results:After treatment,the exercise endurance score in the lung rehabilitation group(335.67±45.29)was higher than that in the non-rehabilitation group(P<0.05).The dyspnea score in the lung rehabilitation group(0.72±0.16)was lower than that in the non-rehabilitation group(P<0.05).Additionally,FVC(3.18±0.58 L),FEV1(2.28±0.56 L),FEV1/FVC(69.69±5.56),and DLCO(60.53±5.92 mL/mmHg/min)were higher in the lung rehabilitation group compared to the non-rehabilitation group after treatment(P<0.05).Conclusion:Lung rehabilitation therapy combined with N-acetylcysteine treatment can effectively improve dyspnea symptoms,lung function,and exercise endurance in patients with RA-ILD.This approach helps to improve patient’s quality of life and is beneficial for their prognosis.

欧盟ENDURE研究计划及其对中国农业工程化研发的启示

农业功能的拓展使农业科研多学科合作成为必然。欧盟成立之来,积极探索成员国间在农业生产、农产品供给、农产品价格和农业产业结构调整等方面一体化发展,确保了欧盟食品安全生产和农业竞争力。其中ENDURE研究计划为适应欧洲农业高度多样化的需求,通过集群创新整体优化欧洲农作物保护策略,集成交叉学科探索生态工程、景观生态学和品种对区域农业条件的适应,使欧洲农业生产更加稳定和可预测。多学科集成研究的科学组织方式也为中国农业工程化研发提供了参考。

Study on Agronomic Characters and Storage Durability of a New Line of Endurable Storage Hybrid Rice Chucangyou No.1

[Objective] The aim of this study was to investigate the storage durability of a new line of endurable storage hybrid rice Chucangyou No.1 selected by endurable storage genes of Yunhui 290.[Method] Agronomic characters of the new line and the control II you 838 and Shanyou 63 were comparatively studied,and storage durability of the new line and indica,japonica or hybrid rice were also comparatively analyzed by the accelerated ageing test.[Result] Agronomic characters of the new line were excellent,and germination rate in the artificial accelerated ageing test was significantly higher than those in other materials.[Conclusion] Chucangyou No.1 has the better storage durability,which can be used in large scale of extention.

《大学英语》(上外版)系列辅导(2)

Unit 3 The Present Language Points: 1.rare:a.稀有的,罕见的,不常发生的。例如: (1)The panda is now a rare animal.熊猫现在是稀有动物。 (2)It is rare for her to go to class late.她上课很少迟到。

废餐油催化裂化制备生物燃料的工艺研究

利用催化裂化方法,以废餐油为原料,研究了不同反应温度、不同催化剂和掺炼不同比例的海南炼化减三线油对制备生物燃料的影响。采用固定流化床装置进行催化裂化反应,测定生成油组分和含量,再生烟气,PONA和炼厂气中各组分的含量。实验结果表明:在废餐油催化试验中,Endurance比CGP-1HN的产物轻质油、总收率高,分别为65%和97%;CGP-1HN液化气收率较高为33%,且其催化活性更为稳定,转化率基本保持在98%。反应温度对催化剂为Endurance的影响较明显,而对催化剂CGP-1HN,反应温度没有明显的影响。海南炼化减三线油中掺入废餐油对海南炼化减三线油的催化裂化产品分布无明显影响,故废餐油是一种良好的催化裂化原料油。

分叉型腹主动脉覆膜支架可疑不良事件相关数据分析

目的:分析Endurant IIs分叉型腹主动脉覆膜支架植入所致可疑不良事件数据,为临床合理、安全使用该器械提供有效信息。方法:通过检索美国食品药品监督管理局(FDA)制造商和用户设备体验(MAUDE)数据库,获取Endurant IIs分叉型腹主动脉覆膜支架植入的219例不良事件数据,并进行统计分析。结果:Endurant IIs分叉型腹主动脉覆膜支架器械故障主要表现为泄漏(占48.37%),对患者产生的影响主要是造成损伤(占70.3%),其不良事件在研究统计的每个月份分布较为均匀。同时分析了引起内漏、血管栓塞和肢体疼痛的可能原因。结论:为有效控制分叉型腹主动脉覆膜支架的不良事件发生,生产企业需努力改进产品性能,临床医务人员要提升自身技术水平和专业技能。

Potential of weed species applied to remediation of soils contaminated with heavy metals

To screen out a series of ideal plants that can effectively remedy contaminated soils by heavy metals is the main groundwork of phytoremediation engineering and the first step of its commercial application on a large scale. In this study, accumulation and endurance of 45 weed species in 16 families from an agricultural site were in situ examined by using the pot-culture field experiment, and the remediation potential of some weed species with high accumulation of heavy metals was assayed. The results showed that Solanum nigrum and Conyza canadensis can not only accumulate high concentration of Cd, but also strongly endure to single Cd and Cd-Pb-Cu-Zn combined pollution. Thus 2 weed species can be regarded as good hyperaccumulators for the remediation of Cd-contaminated soils. Although there were high Cd-accumulation in Artemigia selengensis, Znula britannica and Cephalanoplos setosum, their biomass was adversely affected due to action of heavy metals in the soils. If the problem of low endurance to heavy metals can be solved by a reinforcer, 3 weed species can be perhaps applied commercially.

Endurance exercise and gut microbiota:A review

Background: The physiological and biochemical demands of intense exercise elicit both muscle-based and systemic responses. The main adaptations to endurance exercise include the correction of electrolyte imbalance, a decrease in glycogen storage and the increase of oxidative stress, intestinal permeability, muscle damage, and systemic inflammatory response. Adaptations to exercise might be influenced by the gut microbiota, which plays an important role in the production, storage, and expenditure of energy obtained from the diet as well as in inflammation,redox reactions, and hydration status.Methods: A systematic and comprehensive search of electronic databases, including MEDLINE, Scopus, Clinical Trials.gov, Science Direct,Springer Link, and EMBASE was done. The search process was completed using the keywords: "endurance", "exercise", "immune response","microbiota", "nutrition", and "probiotics".Results: Reviewed literature supports the hypothesis that intestinal microbiota might be able to provide a measureable, effective marker of an athlete's immune function and that microbial composition analysis might also be sensitive enough to detect exercise-induced stress and metabolic disorders. The review also supports the hypothesis that modifying the microbiota through the use of probiotics could be an important therapeutic tool to improve athletes' overall general health, performance, and energy availability while controlling inflammation and redox levels.Conclusion: The present review provides a comprehensive overview of how gut microbiota may have a key role in controlling the oxidative stress and inflammatory responses as well as improving metabolism and energy expenditure during intense exercise.

Prevalence of physical fitness in Chinese school-aged children： Findings from the 2016 Physical Activity and Fitness in China -The Youth Study

Purpose： This study was to present the 2016 prevalence estimates of Chinese school-aged children meeting physical fitness standards and to examine differences by sex and residence locales in children who did not meet fitness standards. Methods： We conducted cross-sectional analyses of 171,991 children and adolescents （boy： 50.0%, Grades 1-12） who participated in the 2016 Physical Activity and Fitness in China--The Youth Study. The main outcomes were fitness measures, assessed by the 2014 revised Chinese National Student Physical Fitness Standard （CNSPFS）, covering areas of aerobic capacity, upper body strength, flexibility, body mass index, abdominal strength, and trunk strength. Children＇s overall physical fitness performance was categorized, per CNSPFS standards, as excellent, good, pass, or no pass. Data on the prevalence of physical fitness categories and not meeting fitness standards （i.e., among children who received a ＂no pass＂ mark） were analyzed, through logistic regression, by sex （boy, girl） and residence locales （urban, rural） across 3 school grades （primary, junior middle, and junior high）. Results： In 2016, 5.95% of Chinese children and adolescents achieved an ＂excellent＂ mark, 25.80% received a ＂good＂ rating, 59.90% received a ＂pass＂, and 8.35% received a ＂no pass＂. Overall, boys were more likely to not pass the fitness standards compared with girls （adjusted odds ratio （aOR） = 1.710; 95% confidence interval （CI）： 1.708-1.712） and children living in urban areas were more likely to not pass the standards than those living in rural areas （aOR = 1.298; 95%CI： 1.296-1.299）. Consistent patterns of not meeting fitness standards were also found by sex and residence locales across all 3 school grades. Conclusion： In the Chinese school-aged population, about 3 in 10 children achieved an ＂excellent＂ or ＂good＂ fitness standard in 2016, and about 8% of this population did not meet CNSPFS standards. Children living in urban areas were more likely to not meet minimum fitness performance levels, and boys in school were more likely to not meet minimum fitness performance levels than girls. 2017 Production and hosting by Elsevier B.V. on behalf of Shanghai University of Sport. This is an open access article under the CC BY-NC-ND license （http：//creativecommons.org/licenses/by-nc-nd/4.0/）.

Endurance tests are the most reliable core stability related measurements

Purpose:To determine the intra-tester reliability of clinical measurements that assess five components related to core stability:strength. endurance,flexibility,motor control,and function. Methods:Participants were 15 college-aged males who had not suffered any orthopedic injury in the past year.Core strength measurements included eight isometric tests and a sit-up test.The four core endurance tests were the trunk flexor test,trunk extensor test,and bilateral side bridge tests.Flexibility tests included the sit-and-reach test and active range of the trunk and hip joint motions.Proprioception via passive reposition tests of the hips and a single limb balance test on an unsteady platform were used to evaluate core motor control.Functional measurements consisted of a squat test and a single leg hop test for time and distance.Measurements were performed during two data collection sessions with a week’s rest between the sessions.Intra-class correlation coefficients were calculated to establish reliability. Results:The overall intra-rater reliability for all core stability related measurements ranged from low(ICC = 0.35,left hip reposition) to very high(ICC = 0.98,sit-and-reach).As a group,the core endurance tests were observed to be the most reliable. Conclusion:There are highly reliable tests in each of the five groups.Overall,core endurance tests are the most reliable measurements,followed by the flexibility,strength,neuromuscular control,and functional tests,respectively.

American ginseng supplementation attenuates creatine kinase level induced by submaximal exercise in human beings

AIM: To investigate whether American ginseng (AG, Panax quinquefolium) supplementation was able to improve endurance exercise performance.METHODS: Thirteen physically active male college students were divided into two groups (AG or placebo)and received supplementation for 4 wk, before the exhaustive running exercise. Treadmill speed was increased to a pace equivalent to 80% VO2max of the subject. A 4-wk washout period followed before the subjects crossed over and received the alternate supplement for the next 4 wk.They then completed a second exhaustive running exercise. The physiological variables that were examined included time to exhaustion and oxygen pulse. Moreover,the plasma creatine kinase (CK) and lactate were measured prior to the exercise, at 15 and 30 min during exercise,immediately after exercise, and 20, 40, 60, and 120 min after exercise.RESULTS: The major finding of this investigation was that the production plasma CK during the exercise significantly decreased for group AG than for group P. Secondary physiological finding was that 80% VO2max running was not improved over a 4-wk AG supplementation regimen.CONCLUSION: Supplementation with AG for 4 wk prior to an exhaustive aerobic treadmill running reduced the leakage of CK during exercise, but did not enhance aerobic work capacity. The reduction of plasma CK may be due to the fact that AG is effective for the decrease of skeletal muscle cell membrane damage, induced by exercise during the high-intensity treadmill run.

Effect of Dissolved Oxygen on Swimming Ability andPhysiological Response to Swimming Fatigue ofWhiteleg Shrimp （Litopenaeus vannamet3

The swimming endurance of whiteleg shrimp （Litopenaeus vannamei, 87.66 mm±0.25 mm, 7.73 g±0.06 g） was exam-ined at various concentrations of dissolved oxygen （DO, 1.9, 3.8, 6.8 and 13.6mgL-1） in a swimming channel against one of the fiveflow velocities （vb v2, v3, v4 and vs）. Metabolite contents in the plasma, hepatopancreas and pleopods muscle of the shrimp werequantified before and after swimming fatigue. The results revealed that the swimming speed and DO concentration were significantfactors that affected the swimming endurance ofL. vannamei. The relationship between swimming endurance and swimming speedat various DO concentrations can be described by the power model （vtb= a）. The relationship between DO concentration （mgL-l） e 9000and the swimming ability index （SAI）, defined as SAI= ∫9000vdt （cm）, can be described as SAI=27.947DO0.137 （R2=0.9312）. Thelevel of DO concentration directly affected the physiology of shrimp, and exposure to low concentrations of DO led to the increasesin lactate and energetic substrate content in the shrimp. In responding to the low DO concentration at 1.9 mg L-1 and the swimmingstress, L. vannamei exhibited a mix of aerobic and anaerobic metabolism to satisfy the energetic demand, mainly characterized by theutilization of total protein and glycogen and the production of lactate and glucose. Fatigue from swimming led to severe loss ofplasma triglyceride at Vl, v2, and v3 with 1.9mgL-1DO, and at V1 with 3.8, 6.8 and 13.6mgL-1 DO, whereas the plasma glucose con-tent increased significantly at v3, v4 and v5 with 3.8 and 6.8mgL-1 DO, and at v5 with 13.6mgL-1 DO. The plasma total protein andhepatopancreas glycogen were highly depleted in shrimp by swimming fatigue at various DO concentrations, whereas the plasmalactate accumulated at high levels after swimming fatigue at different velocities. These results were of particular value to under-standing the locomotory ability of whiteleg shrimp and its physiological changes, further contributing to the improvement of captureand rearing technique.

Reliability evaluation on sense-switch p-channel flash

In this paper,the reliability of sense-switch p-channel flash is evaluated extensively.The endurance result indicates that the p-channel flash could be programmed and erased for more than 10000 cycles;the room temperature read stress shows negligible influence on the p-channel flash cell;high temperature data retention at 150℃ is extrapolated to be about 5 years and 53 years corresponding to 30% and 40% degradation in the drive current,respectively.Moreover,the electrical parameters of the p-channel flash at different operation temperature are found to be less affected.All the results above indicate that the sense-switch p-channel flash is suitable to be used as the configuration cell in flash-based FPGA.

Efficient structural seismic performance evaluation method using improved endurance time analysis

Evaluation of structural performance under seismic excitations from low intensity to high intensity is essential to verify the seismic resistant capacity of a structure, and usually carried out by the incremental dynamic analysis (IDA) method or pushover method. The recently developed endurance time (ET) method is another method that uses dynamic pushover excitations, i.e., endurance time acceleration function, to obtain results similar to those obtained by IDA or pushover methods with low computational cost and acceptable accuracy. This study proposes an improvement on the ET method by considering more restrictions for both the elastic and inelastic response spectra in the generation procedure, and by specifying a target duration. Four reinforced concrete frame structures with 4, 8, 12, and 16 stories are adopted to verify the accuracy of the improved method. Comparison of the results obtained by the proposed method, the ET method and the IDA method shows that the improved method has a higher accuracy than the ET method. For evaluation of structural responses under specifi c ground motion intensity, which is typically required in seismic design codes, the results obtained by the proposed method are compared with fi ve commonly used ground motion selection methods, and shows the proposed method provides acceptable accuracy for engineering applications.

Anti-free radical,anti-oxidative ability and anti-fatigue effects of Huanshaodan An experiment of aging mice

In the theory of traditional Chinese medicine, aging is mainly thought renal deficiency caused renal failure, mainly involving decline of kidney-Yang and deficiency of kidney-essence. Huanshaodan, a Chinese traditional preparation for kidney-replenishing essence, was used to be the preparation for reinforcing renal deficiency and preventing aging for aged people. OBJECTIVE： To observe the effects of Huanshaodan on swimming durance and the abilities of catalase （CAT） in serum and monoamine oxidase-B （MAO-B） in brain tissue as well as in vitro anti-oxidative ability of aging mouse. DESIGN： A controlled animal experiment. SETTING： College of Basic Medicine, Hunan University of Traditional Chinese Medicine. MATERIALS： Fifty-four healthy NIH mice, aged 18 months old, of either gender, weighing （48.9±5.4） g, and one SD male rat, aged 16 months old, weighing 51.7 g, were provided by Animal Experimental Center, Hunan University of Traditional Chinese Medicine. Thirty NIH mice were randomly chosen for swimming test, and divided into experimental group and control group, with 15 in each; The other 24 NIH mice were used for enzyme activity assay, and also divided into experimental group and control group, with 12 in each. SD rat was used for in vitro anti-oxidative ability test, Huanshaodan water decoction was composed of Cheqianzi, Wuweizi, Huaishan, Danggui, Huangbai, Shudi, Baizhi, Niuxi, Baishen, Tusizi, Buguzhi, Roucongrong and Heshouwu 13 Chinese herbs. METHODS： This study was carried out in the Second Laboratory, Department of Biochemistry, Hunan University of Traditional Chinese Medicine in June 2006. Swimming and enzyme activity assay： Mice in the two experimental groups were intragastrically administrated with l0 μL/g Huanshaodan water decoction. Mice in the two control groups were intragastrically administrated with the same amount of normal saline. All the mice were intragastrically administrated for 5 days, and they were free to access to medicine in the other 2 days in a week. Each mouse was administrated for 8 weeks. MAIN OUTCOME MEASURES： ① Forty days after administration, mice in the experimental group and control group for swimming test were loaded at tails and allowed to swim in the water-tank. Swimming durance was recorded. ② Following the method of Chen Qi, the activities of CAT in serum and MAO-B in brain tissue as well as the inhibitory rate of each medicine on malonaldehyde （MDA） content in the in vitro rat hepatic tissue were determined; Meanwhile, the inhibitory rate of different doses of Tusizi liquid to MDA content in the rat hepatic tissue in vitro was also assayed. RESULTS： Fifty-four NIH mice and one SD rat were recruited in this experiment. Three mice died in the swimming test, and all the other animals were involved in the final analysis. ① Swimming durance of mice in the experimental group was significantly longer than that in the control group （ t =7.502, P 〈 0.01 ） . The activity of CAT in serum of mice in the experimental group was significantly higher than that in the control group （ t =13.307, P 〈 0.01 ） . ② The activity of MAO-B in brain tissue of mice in the experimental group was significantly lower than that in the control group （t =l3.27, P 〈 0.01 ） . ③The inhibitory rate of Cheqianzi, Wuweizi, Huaishan, Danggui, Huangbai, Shudi, Baizhi, Niuxi, Baishen, Tusizi, Buguzhi, Roucongrong and Heshouwu 13 Chinese herbs of Huanshaodan to MDA in the rat hepatic tissue in vitro was - 62.9, - 95.1, - 34.9, - 65.1, - 99.1, - 87.2, - 94.1, - 20.0, - 67.0, - 83.7, - 91.0, - 98.4, - 93.0, respectively. ④ The inhibitory rate of low to high dose of Tusizi liquid to MDA content in the rat hepatic tissue in vitro was - 3.41, - 18.1, - 26.6, - 83.7, respectively. CONCLUSION： Huanshaodan enhances swimming endurance, anti-oxygen free radical and anti-oxidativeabilities, and thus, it can delay aging.