Objective:To evaluate the antibacterial activity of sea snake(Enhydrina schistosa)venom protein against drug-resistant human pathogenic bacterial strains.Methods:The venom was collected by milking process from the liv...Objective:To evaluate the antibacterial activity of sea snake(Enhydrina schistosa)venom protein against drug-resistant human pathogenic bacterial strains.Methods:The venom was collected by milking process from the live specimens of sea snake are using capillary tubes or glass plates.Venom was purified by ion exchange chromatography and it was tested for in-vitro antibacterial activity against 10 drug-resistant human pathogenic bacterial strains using the standard disc diffusion method.Results:The notable antibacterial activity was observed at 150μg/mL concentration of purified venom and gave its minimum inhibitory concentrations values exhibited between 200-100μg/mL against all the tested bacterial strains.The maximum zone of inhibition was observed at 16.4 mm against Salmonella boydii and the minimum activity was observed at 7.5 mm against Pseudomonas aeruginosa.After the sodium-dodecyl-sulfate-polyacrylamide gel electrophoresis there were a clear single band was detected in the gel that corresponding to purified venom protein molecular weight of 44 kDa.Conclusions:These results suggested that the sea snake venom might be a feasible source for searching potential antibiotics agents against human pathogenic diseases.展开更多
基金Supported by Ministry of Earth Science(MoES major project reference No.MOSE/9-DS/06/2007/Pc-IV-IV),Govt.of India,for providing financial supports under the scheme“Drugs from the sea”programs.
文摘Objective:To evaluate the antibacterial activity of sea snake(Enhydrina schistosa)venom protein against drug-resistant human pathogenic bacterial strains.Methods:The venom was collected by milking process from the live specimens of sea snake are using capillary tubes or glass plates.Venom was purified by ion exchange chromatography and it was tested for in-vitro antibacterial activity against 10 drug-resistant human pathogenic bacterial strains using the standard disc diffusion method.Results:The notable antibacterial activity was observed at 150μg/mL concentration of purified venom and gave its minimum inhibitory concentrations values exhibited between 200-100μg/mL against all the tested bacterial strains.The maximum zone of inhibition was observed at 16.4 mm against Salmonella boydii and the minimum activity was observed at 7.5 mm against Pseudomonas aeruginosa.After the sodium-dodecyl-sulfate-polyacrylamide gel electrophoresis there were a clear single band was detected in the gel that corresponding to purified venom protein molecular weight of 44 kDa.Conclusions:These results suggested that the sea snake venom might be a feasible source for searching potential antibiotics agents against human pathogenic diseases.
