Two new ent-kauranoids, tenuifolin A (3beta,6alpha, 15beta-trihydroxy-1alpha, 7beta-diacetoxy-11beta, 16beta-epoxy-ent-kaurane) (1) and tenuifolin B (1alpha,6alpha, 11beta-trihydroxy-3beta,7beta-diacetoxy-ent-kaur-16-...Two new ent-kauranoids, tenuifolin A (3beta,6alpha, 15beta-trihydroxy-1alpha, 7beta-diacetoxy-11beta, 16beta-epoxy-ent-kaurane) (1) and tenuifolin B (1alpha,6alpha, 11beta-trihydroxy-3beta,7beta-diacetoxy-ent-kaur-16-en-15-one) (2), together with four known compounds were isolated from the aerial parts of Isodon tenuifolia (W. W. Smith) Kudo collected from Zhongdian County, Yunnan Province, China. Their structures were determined by the spectral methods (including 2D NMR techniques).展开更多
Constitutively active Wnt signaling frequently occurs in most colon cancers.Therefore,inhibitors of Wnt signaling pathway could provide rational therapeutic effects for colorectal malignancy.Within this paper,we ident...Constitutively active Wnt signaling frequently occurs in most colon cancers.Therefore,inhibitors of Wnt signaling pathway could provide rational therapeutic effects for colorectal malignancy.Within this paper,we identified two inhibitors of Wnt signaling pathway,rabdoternin B and maoecrystal I from a natural ent-kauranoid library by a dualluciferase reporter gene assay.The two compounds inhibited Wnt signaling pathway in a concentration-dependent manner and exhibited selective cytotoxicity toward a number of colon carcinoma cell lines SW480,HCT116,and HT29,with only weak cytotoxicity towards the normal colonic epithelial cell line CCD-841-CoN.Rabdoternin B and maoecrystal I treatment induced G2/M phase arrest efficiently in SW480 cells as revealed by flow cytometry analysis.A further study found that maoecrystal I decreased the expression of Wnt signaling target genes,including c-myc,cyclin D1,survivin and Axin2 in colon cancer cells.Collectively our data suggests that rabdoternin B and maoecrystal I are novel inhibitors of canonical Wnt signaling pathway and may possess potentials for colon cancer therapy.展开更多
Two new ent-kaurane diterpenoids, sculponeatins L (1) and M (2), were isolated from the EtOAc extract of Isodon sculponeata. Their structures were elucidated by spectroscopic evidences. The cytotoxicities of 1 and 2...Two new ent-kaurane diterpenoids, sculponeatins L (1) and M (2), were isolated from the EtOAc extract of Isodon sculponeata. Their structures were elucidated by spectroscopic evidences. The cytotoxicities of 1 and 2 against human tumor cells K562 and T24 were tested.展开更多
Objective To study the ent-kaurane diterpenoids from Rabdosia rubescens. Methods The compounds were isolated by chromatographies and their structures were identified by spectral analyses. Results Four compounds were i...Objective To study the ent-kaurane diterpenoids from Rabdosia rubescens. Methods The compounds were isolated by chromatographies and their structures were identified by spectral analyses. Results Four compounds were isolated, and they were identified as bisrubescensin E (1), 2α,3α,24-trihydroxyurs-12-en-28-oic acid (2), 2α,3α,24-trihydroxyurs-12,20-(30)-dien-28-oic acid (3), and 6,7-dihydroxycoumarin (4). Conclusion Compound 1 is a new asymmetric ent-kauranoid dimer. Compound 2 is isolated from the plant for the first time. Compounds 3 and 4 are isolated from the plants of Rabdosia (Bl.) Hassk for the first time.展开更多
Two new ent-kauranoids, named maoyecrystals A (1) and B (2), were isolated from the EtOAc extract of the dried leaves of Isodon japonica (Burman f.) Hara collected in Tongbai mountains, Henan Province. Their structur...Two new ent-kauranoids, named maoyecrystals A (1) and B (2), were isolated from the EtOAc extract of the dried leaves of Isodon japonica (Burman f.) Hara collected in Tongbai mountains, Henan Province. Their structures were determined on the basis of spectral data, especially by 2D NMR.展开更多
文摘Two new ent-kauranoids, tenuifolin A (3beta,6alpha, 15beta-trihydroxy-1alpha, 7beta-diacetoxy-11beta, 16beta-epoxy-ent-kaurane) (1) and tenuifolin B (1alpha,6alpha, 11beta-trihydroxy-3beta,7beta-diacetoxy-ent-kaur-16-en-15-one) (2), together with four known compounds were isolated from the aerial parts of Isodon tenuifolia (W. W. Smith) Kudo collected from Zhongdian County, Yunnan Province, China. Their structures were determined by the spectral methods (including 2D NMR techniques).
基金the hundreds top talents program from Chinese Academy of Sciences,the NSFC(No.81173076,21322204)the projects of science and technology of Yunnan Province(2009C1120,2013FA047).
文摘Constitutively active Wnt signaling frequently occurs in most colon cancers.Therefore,inhibitors of Wnt signaling pathway could provide rational therapeutic effects for colorectal malignancy.Within this paper,we identified two inhibitors of Wnt signaling pathway,rabdoternin B and maoecrystal I from a natural ent-kauranoid library by a dualluciferase reporter gene assay.The two compounds inhibited Wnt signaling pathway in a concentration-dependent manner and exhibited selective cytotoxicity toward a number of colon carcinoma cell lines SW480,HCT116,and HT29,with only weak cytotoxicity towards the normal colonic epithelial cell line CCD-841-CoN.Rabdoternin B and maoecrystal I treatment induced G2/M phase arrest efficiently in SW480 cells as revealed by flow cytometry analysis.A further study found that maoecrystal I decreased the expression of Wnt signaling target genes,including c-myc,cyclin D1,survivin and Axin2 in colon cancer cells.Collectively our data suggests that rabdoternin B and maoecrystal I are novel inhibitors of canonical Wnt signaling pathway and may possess potentials for colon cancer therapy.
文摘Two new ent-kaurane diterpenoids, sculponeatins L (1) and M (2), were isolated from the EtOAc extract of Isodon sculponeata. Their structures were elucidated by spectroscopic evidences. The cytotoxicities of 1 and 2 against human tumor cells K562 and T24 were tested.
文摘Objective To study the ent-kaurane diterpenoids from Rabdosia rubescens. Methods The compounds were isolated by chromatographies and their structures were identified by spectral analyses. Results Four compounds were isolated, and they were identified as bisrubescensin E (1), 2α,3α,24-trihydroxyurs-12-en-28-oic acid (2), 2α,3α,24-trihydroxyurs-12,20-(30)-dien-28-oic acid (3), and 6,7-dihydroxycoumarin (4). Conclusion Compound 1 is a new asymmetric ent-kauranoid dimer. Compound 2 is isolated from the plant for the first time. Compounds 3 and 4 are isolated from the plants of Rabdosia (Bl.) Hassk for the first time.
文摘Two new ent-kauranoids, named maoyecrystals A (1) and B (2), were isolated from the EtOAc extract of the dried leaves of Isodon japonica (Burman f.) Hara collected in Tongbai mountains, Henan Province. Their structures were determined on the basis of spectral data, especially by 2D NMR.
