This work aims to understand the effect of nanoparticle-enzyme interactions and how such interactions affect starch based soil removal. Silica and laponite are used as the model nanoparticles, and s-amylase is employe...This work aims to understand the effect of nanoparticle-enzyme interactions and how such interactions affect starch based soil removal. Silica and laponite are used as the model nanoparticles, and s-amylase is employed as the model enzyme. The results show that, if the nanoparticles and enzyme are added simultaneously, laponite enhances the enzyme performance toward starch soil removal, whereas silica imposes a small effect on the enzymatic activity towards the same soil substrates. However, when nanoparticles are added first, the enzyme activity is not affected much by laponite but is hindered significantly by silica nanoparticles. Furthermore, sequential addition of the enzyme followed by silica nanoparticles improves soil removal. Electron microscopic analyses, measurements of the enzyme activity in suspen- sions of nanoparticles, and particle size characterisation suggest that dense coverage of soil surface by the silica nanoparticles be likely a mechanism for the experimentally observed hindrance of soil removal when silica nanoparticles are added before enzyme.展开更多
基金UK EPSRC for funding this project under Grants EP EP/F027389/1,EP/F023014/1,EP/D000645/1 and EP/F000464/1
文摘This work aims to understand the effect of nanoparticle-enzyme interactions and how such interactions affect starch based soil removal. Silica and laponite are used as the model nanoparticles, and s-amylase is employed as the model enzyme. The results show that, if the nanoparticles and enzyme are added simultaneously, laponite enhances the enzyme performance toward starch soil removal, whereas silica imposes a small effect on the enzymatic activity towards the same soil substrates. However, when nanoparticles are added first, the enzyme activity is not affected much by laponite but is hindered significantly by silica nanoparticles. Furthermore, sequential addition of the enzyme followed by silica nanoparticles improves soil removal. Electron microscopic analyses, measurements of the enzyme activity in suspen- sions of nanoparticles, and particle size characterisation suggest that dense coverage of soil surface by the silica nanoparticles be likely a mechanism for the experimentally observed hindrance of soil removal when silica nanoparticles are added before enzyme.
