Effects of rising temperature on growth and energy budget of juvenile Eogammarus possjeticus(Amphipoda:Anisogammaridae)

Growth and energy budget of marine amphipod juvenile Eogammarus possjeticus at different temperatures(20℃,24℃,26℃,28℃,30℃,32℃ and 34℃)were investigated in this study.The results showed that the cumulative mortality rate increased significantly with rising temperature(p<0.01),and exceeded 50%after 24 h when temperature was above 30℃.With the temperature increasing from 20℃ to 26℃,the ingestion rate and absorption rate increased,but decreased significantly above 28℃(p<0.01),indicating a decline in feeding ability at high temperatures.The specific growth rate increased with rising temperature,but decreased significantly(p<0.01)after reaching the maximum value at 24℃.Similarly,the oxygen consumption and ammonia emission rates also showed a trend of first increase and then decrease.However,the O:N ratio decreased first and then increased with rising temperature,indicating that the energy demand of E.possjeticus juvenile transferred from metabolism of carbohydrate and lipid to protein.In the energy distribution of amphipods,the proportion of each energy is different.With rising temperature,the ratio of the energy deposited for growth accounted for ingested gross energy showing a trend of decrease,while the energy lost to respiration,ammonia excretion,and feces accounted for ingested gross energy being showed a trend of increase.It seemed that rising temperature increased the metabolism and energy consumption of the amphipods and,meanwhile,decreased the energy used for growth,which may be an important reason for the slow growth and small body size of the amphipods during the summer high-temperature period.

Effects of salinity, carbonate alkalinity, and pH on physiological indicators of nutrition transporter for potential habitat restoration of amphipod Eogammarus possjeticus

The effects of three environmental factors,salinity,carbonate alkalinity,and pH,on the survival,feeding,and respiratory metabolism of Eogammarus possjeticus(Amphipoda:Gammaridae)were investigated experimentally.The results show that E.possjeticus could tolerate a broad salinity range.The 24-h lowest median lethal salinity was 2.70,and the highest was 47.33.The 24-h median lethal alkalinity and pH were 23.05 mmol/L and 9.91,respectively;both values decreased gradually with time.Different values of salinity,carbonate alkalinity,and pH resulted in significant differences in the cumulative mortality(P<0.05).The ingestion rate and feed absorption efficiency were significantly affected by the coupling of the three environmental factors(P<0.05).With increases in carbonate alkalinity,salinity,and pH,both ingestion rate and feed absorption efficiency exhibited a downward trend,indicating a decline in feeding ability under high salinity and more alkaline water conditions.The coupling of salinity,carbonate alkalinity,and pH also had a significant effect on respiration and excretion(P<0.05).The oxygen consumption rate increased first and then decreased with increasing carbonate alkalinity.Under the same carbonate alkalinity values,the oxygen consumption rate increased with increasing salinity.Under the same carbonate alkalinity and salinity,the oxygen consumption rate initially increased and then decreased with increasing pH.The O:N ratio first increased and then decreased with increasing carbonate alkalinity.When carbonate alkalinity was less than 6 mmol/L,the O:N ratio increased with increasing salinity and decreased with increasing pH.The results demonstrate that changes in salinity,carbonate alkalinity,and pH had a measurable impact on the osmotic pressure equilibrium in E.possjeticus and affected the energy supply mode(i.e.ratio of metabolic substrate).