AIM： To produce an antibody against rat eosinophil cationic protein （ECP） and to examine the effects of the antibody in rats with dextran sulfate sodium （DSS）-induced colitis. METHODS： An antibody was raised aga...AIM： To produce an antibody against rat eosinophil cationic protein （ECP） and to examine the effects of the antibody in rats with dextran sulfate sodium （DSS）-induced colitis. METHODS： An antibody was raised against rat ECP. Rats were treated with 3% DSS in drinking water for 7 d and received the antibody or normal serum. The colons were examined histologically and correlated with clinical symptoms. Immunohistochemistry and Western blot analysis were estimated as a grade of inflammation. RESULTS： The ECP antibody stained the activated eosinophils around the injured crypts in the colonic mucosa. Antibody treatment reduced the severity of colonic ulceration and acute clinical symptoms （diarrhea and/or bloodstained stool）. Body weight gain was significantly greater and the colon length was significantly longer in anti-ECPtreated rats than in normal serum-treated rats. Expression of ECP in activated eosinophils was associated with the presence of erosions and inflammation. The number of Ki-67-positive cells in the regenerated surface epithelium increased in anti-ECP-treated rats compared with normal serum-treated rats. Western blot analysis revealed reduced expression of macrophage migration inhibitory factor （MIF） in anti-ECP-treated rats. CONCLUSION： Our results indicate that treatment with ECP antibody, improved DSS-induced colitis in rats, possibly by increasing the regenerative activity of the colonic epithelium and downregulation of the immune response, and suggest that anti-ECP may promote intestinal wound healing in patients with ulcerative colitis （UC）.展开更多
目的 分析嗜酸性粒细胞阳离子蛋白(ECP)/髓过氧化物酶(MPO)、高迁移率族蛋白B1(HMGB1)与鼻内窥镜微创手术患者术后症状控制效果的关系。方法 选取2020年8月至2022年4月新疆维吾尔自治区人民医院耳鼻喉诊疗中心门诊慢性鼻窦炎患者274例...目的 分析嗜酸性粒细胞阳离子蛋白(ECP)/髓过氧化物酶(MPO)、高迁移率族蛋白B1(HMGB1)与鼻内窥镜微创手术患者术后症状控制效果的关系。方法 选取2020年8月至2022年4月新疆维吾尔自治区人民医院耳鼻喉诊疗中心门诊慢性鼻窦炎患者274例为研究对象,其中随机选取137例作为训练组,其余137例作为验证组。均采用鼻内窥镜微创手术治疗。比较训练组慢性鼻窦炎患者术前及术后7 d ECP/MPO、HMGB1水平,统计术后3个月症状控制情况。分析训练组慢性鼻窦炎患者术后症状控制效果的影响因素,绘制受试者工作特征(ROC)曲线分析训练组ECP/MPO、HMGB1对慢性鼻窦炎患者术后症状控制效果的评估价值并进行决策曲线分析(DCA)。采用验证组数据评价指标的预测能力。结果 训练组患者术后7 d ECP/MPO、HMGB1水平低于术前(P<0.05)。二元logistic回归分析,结果显示,术后7 d Lund-Kennedy评分、五项主观症状视觉模拟量表(VAS)总评分、ECP/MPO、HMGB1水平是训练组经鼻内窥镜微创手术治疗的慢性鼻窦炎患者术后症状控制效果的独立影响因素(P<0.05)。训练组ECP/MPO、HMGB1联合检测的AUC值高于ECP/MPO、HMGB1单独评估的AUC值(χ2=3.036、2.976,P=0.002、0.003),净受益率最大为0.84,当风险阈值为0.10~0.72时,ECP/MPO、HMGB1联合检测的净受益率大于单独检测。验证组ECP/MPO、HMGB1水平联合预测症状控制效果敏感度为79.12%,特异度为76.09%,阴性预测值为64.81%,阳性预测值为86.75%。结论 鼻内窥镜微创手术患者ECP/MPO、HMGB1表达情况与术后症状控制效果关系密切,术后早期检测二者水平可为临床预测预后提供参考。展开更多
基金Supported by a grant-in-aid from the Ministry of Science, Education, Sports and Culture of Japan, No. 14570193
文摘AIM： To produce an antibody against rat eosinophil cationic protein （ECP） and to examine the effects of the antibody in rats with dextran sulfate sodium （DSS）-induced colitis. METHODS： An antibody was raised against rat ECP. Rats were treated with 3% DSS in drinking water for 7 d and received the antibody or normal serum. The colons were examined histologically and correlated with clinical symptoms. Immunohistochemistry and Western blot analysis were estimated as a grade of inflammation. RESULTS： The ECP antibody stained the activated eosinophils around the injured crypts in the colonic mucosa. Antibody treatment reduced the severity of colonic ulceration and acute clinical symptoms （diarrhea and/or bloodstained stool）. Body weight gain was significantly greater and the colon length was significantly longer in anti-ECPtreated rats than in normal serum-treated rats. Expression of ECP in activated eosinophils was associated with the presence of erosions and inflammation. The number of Ki-67-positive cells in the regenerated surface epithelium increased in anti-ECP-treated rats compared with normal serum-treated rats. Western blot analysis revealed reduced expression of macrophage migration inhibitory factor （MIF） in anti-ECP-treated rats. CONCLUSION： Our results indicate that treatment with ECP antibody, improved DSS-induced colitis in rats, possibly by increasing the regenerative activity of the colonic epithelium and downregulation of the immune response, and suggest that anti-ECP may promote intestinal wound healing in patients with ulcerative colitis （UC）.
文摘目的 分析嗜酸性粒细胞阳离子蛋白(ECP)/髓过氧化物酶(MPO)、高迁移率族蛋白B1(HMGB1)与鼻内窥镜微创手术患者术后症状控制效果的关系。方法 选取2020年8月至2022年4月新疆维吾尔自治区人民医院耳鼻喉诊疗中心门诊慢性鼻窦炎患者274例为研究对象,其中随机选取137例作为训练组,其余137例作为验证组。均采用鼻内窥镜微创手术治疗。比较训练组慢性鼻窦炎患者术前及术后7 d ECP/MPO、HMGB1水平,统计术后3个月症状控制情况。分析训练组慢性鼻窦炎患者术后症状控制效果的影响因素,绘制受试者工作特征(ROC)曲线分析训练组ECP/MPO、HMGB1对慢性鼻窦炎患者术后症状控制效果的评估价值并进行决策曲线分析(DCA)。采用验证组数据评价指标的预测能力。结果 训练组患者术后7 d ECP/MPO、HMGB1水平低于术前(P<0.05)。二元logistic回归分析,结果显示,术后7 d Lund-Kennedy评分、五项主观症状视觉模拟量表(VAS)总评分、ECP/MPO、HMGB1水平是训练组经鼻内窥镜微创手术治疗的慢性鼻窦炎患者术后症状控制效果的独立影响因素(P<0.05)。训练组ECP/MPO、HMGB1联合检测的AUC值高于ECP/MPO、HMGB1单独评估的AUC值(χ2=3.036、2.976,P=0.002、0.003),净受益率最大为0.84,当风险阈值为0.10~0.72时,ECP/MPO、HMGB1联合检测的净受益率大于单独检测。验证组ECP/MPO、HMGB1水平联合预测症状控制效果敏感度为79.12%,特异度为76.09%,阴性预测值为64.81%,阳性预测值为86.75%。结论 鼻内窥镜微创手术患者ECP/MPO、HMGB1表达情况与术后症状控制效果关系密切,术后早期检测二者水平可为临床预测预后提供参考。
