含溴结构域蛋白7(bromodomain-containing protein 7,BRD7)是含溴结构域蛋白家族成员之一,该类蛋白因含有高度保守的溴区结构域得名[1]。BRD7通过参与信号依赖的基因转录过程从而广泛参与并调控细胞增殖、分化、迁移、乏氧及凋亡等过程[...含溴结构域蛋白7(bromodomain-containing protein 7,BRD7)是含溴结构域蛋白家族成员之一,该类蛋白因含有高度保守的溴区结构域得名[1]。BRD7通过参与信号依赖的基因转录过程从而广泛参与并调控细胞增殖、分化、迁移、乏氧及凋亡等过程[2-4]。编码BRD7蛋白的相关基因是由余鹰等[5]在鼻咽癌的活检组织标本中通过cDNA差异分析法克隆得到的一个基因。展开更多
AIM: APMCF1 is a novel human gene whose transcripts are up-regulated in apoptotic MCF-7 cells. In order to learn more about this gene′s function in other tumors, we cloned its full length cDNA and prepared its polycl...AIM: APMCF1 is a novel human gene whose transcripts are up-regulated in apoptotic MCF-7 cells. In order to learn more about this gene′s function in other tumors, we cloned its full length cDNA and prepared its polyclonal antibody to investigate its expression in colon cancers with immunohistochemistry.METHODS: With the method of 5′ rapid amplification of cDNA end (RACE) and EST assembled in GenBank, we extended the length of APMCF1 at 5′ end. Then the sequence encoding the APMCF1 protein was amplified by RT-PCR from the total RNA of apoptotic MCF-7 cells and cloned into the prokaryotic expression vector pGEX-KG to construct recombinant expression vector pGEX-APMCF1. The GSTAPMCF1 fusion protein was expressed in E. coli and used to immunize rabbits to get the rabbit anti-APMCF1 serum. The specificity of polyclonal anti-APMCF1 antibody was determined by Western blot. Then we investigated the expression of Apmcf1 in colon cancers and normal colonic mucosa with immunohistochemistry.RESULTS: A cDNA fragment with a length of 1 745 bp was obtained. APMCF1 was mapped to chromosome 3q22.2and spanned at least 14.8 kb of genomic DNA with seven exons and six introns contained. Bioinformatic analysis showed the protein encoded by APMCF1 contained a small GTP-binding protein (G proteins) domain and was homologous to mouse signal recognition particle receptor β(SRβ). A coding region covering 816 bp was cloned and polyclonal anti-APMCF1 antibody was prepared successfully.The immunohistochemistry study showed that APMCF1 had a strong expression in colon cancer.CONCLUSION: APMCF1 may be the gene coding human signal recognition particle receptor β and belongs to the small-G protein superfamily. Its strong expression pattern in colon cancer suggests it may play a role in colon cancer development.展开更多
E1AF基因是ETS（E26 transforming specific or E twenty six specific）癌基因家族的新成员，是鼠PEA3癌基因的同源类似物。以往研究发现，ElAF苯因对恶性肿瘤的进展有促进作用，其作用可能与基质金属蛋白酶（matrix metalloproteinase...E1AF基因是ETS（E26 transforming specific or E twenty six specific）癌基因家族的新成员，是鼠PEA3癌基因的同源类似物。以往研究发现，ElAF苯因对恶性肿瘤的进展有促进作用，其作用可能与基质金属蛋白酶（matrix metalloproteinase，MMP）有关。本研究的目的在于阐明E1AF基因对大肠癌侵袭能力的影响，并同时研究E1AF对MMP。2和MMP-7的调控，以明确E1AF基因促进大肠癌侵袭能力的机制。展开更多
文摘含溴结构域蛋白7(bromodomain-containing protein 7,BRD7)是含溴结构域蛋白家族成员之一,该类蛋白因含有高度保守的溴区结构域得名[1]。BRD7通过参与信号依赖的基因转录过程从而广泛参与并调控细胞增殖、分化、迁移、乏氧及凋亡等过程[2-4]。编码BRD7蛋白的相关基因是由余鹰等[5]在鼻咽癌的活检组织标本中通过cDNA差异分析法克隆得到的一个基因。
基金National Natural Science Foundation of China,No.30270667
文摘AIM: APMCF1 is a novel human gene whose transcripts are up-regulated in apoptotic MCF-7 cells. In order to learn more about this gene′s function in other tumors, we cloned its full length cDNA and prepared its polyclonal antibody to investigate its expression in colon cancers with immunohistochemistry.METHODS: With the method of 5′ rapid amplification of cDNA end (RACE) and EST assembled in GenBank, we extended the length of APMCF1 at 5′ end. Then the sequence encoding the APMCF1 protein was amplified by RT-PCR from the total RNA of apoptotic MCF-7 cells and cloned into the prokaryotic expression vector pGEX-KG to construct recombinant expression vector pGEX-APMCF1. The GSTAPMCF1 fusion protein was expressed in E. coli and used to immunize rabbits to get the rabbit anti-APMCF1 serum. The specificity of polyclonal anti-APMCF1 antibody was determined by Western blot. Then we investigated the expression of Apmcf1 in colon cancers and normal colonic mucosa with immunohistochemistry.RESULTS: A cDNA fragment with a length of 1 745 bp was obtained. APMCF1 was mapped to chromosome 3q22.2and spanned at least 14.8 kb of genomic DNA with seven exons and six introns contained. Bioinformatic analysis showed the protein encoded by APMCF1 contained a small GTP-binding protein (G proteins) domain and was homologous to mouse signal recognition particle receptor β(SRβ). A coding region covering 816 bp was cloned and polyclonal anti-APMCF1 antibody was prepared successfully.The immunohistochemistry study showed that APMCF1 had a strong expression in colon cancer.CONCLUSION: APMCF1 may be the gene coding human signal recognition particle receptor β and belongs to the small-G protein superfamily. Its strong expression pattern in colon cancer suggests it may play a role in colon cancer development.
文摘E1AF基因是ETS（E26 transforming specific or E twenty six specific）癌基因家族的新成员，是鼠PEA3癌基因的同源类似物。以往研究发现，ElAF苯因对恶性肿瘤的进展有促进作用，其作用可能与基质金属蛋白酶（matrix metalloproteinase，MMP）有关。本研究的目的在于阐明E1AF基因对大肠癌侵袭能力的影响，并同时研究E1AF对MMP。2和MMP-7的调控，以明确E1AF基因促进大肠癌侵袭能力的机制。
