Objectives. To analyze the expressions of the protein and mRNA of Eph A2 and Ep hrinA-1 in squamous cell cervical carcinomas and explore their prognostic value in cervical carcinoma. Methods. Immunohistochemistry was ...Objectives. To analyze the expressions of the protein and mRNA of Eph A2 and Ep hrinA-1 in squamous cell cervical carcinomas and explore their prognostic value in cervical carcinoma. Methods. Immunohistochemistry was used to assess the pro tein expressions of EphA2 and EphrinA-1 in 206 patients with squamous cervical carcinoma FIGO stage Ia-IVb. Frozen tissues from 20 cases in which the tumors s howed variable EphA2 and EphrinA-1protein expressions were used for laser captu re microdissection (LCM). About 50 cancer cells in each frozen section were capt ured with the LCM method and processed for RT-PCR detection of EphA2 and Ephrin A-1 mRNA. Results. Among the 206 squamous cervical carcinomas, 23 (11.2%) show ed negative, 94(45.6%) weakly positive, 72 (35.0%)moderately positive,and 17 ( 8.3%) strongly positive for EphA2 immunostaining. For EphrinA-1 protein expres sion, 17 tumors (8.3%) showed negative, 95 (46.1%)weakly positive, 71(34.5%) moderately positive, and 23 (11.2%) strongly positive. EphA2 and EphrinA-1 oft en colocalized in the same tumor areas and vascular endothelial cells. Variable amount of mRNA expressions of EphA2 and EphrinA-1 were observed in the 20 tumor s analyzed. There was no significant correlation between the overexpressions of EphA2/EphrinA-1 and age and FIGO stage. High level of EphA2 was significantly a ssociated with overall survival in univariate and multivariate analysis. Moderat e to high EphrinA-1 protein expression was significantly associated with overal l survival in multivariate analysis. The combined high level of expression of Eh pA2 and moderate to high level of expression of EphrinA-1 was a strong predicto r of overall survival. Conclusions. High levels of EphA2 together with moderate to high level of EphrinA-1 protein expressions in squamous cervical carcinoma w ere predictive for a shorter overall survival and these proteins may be valuable prognostic markers.展开更多
文摘Objectives. To analyze the expressions of the protein and mRNA of Eph A2 and Ep hrinA-1 in squamous cell cervical carcinomas and explore their prognostic value in cervical carcinoma. Methods. Immunohistochemistry was used to assess the pro tein expressions of EphA2 and EphrinA-1 in 206 patients with squamous cervical carcinoma FIGO stage Ia-IVb. Frozen tissues from 20 cases in which the tumors s howed variable EphA2 and EphrinA-1protein expressions were used for laser captu re microdissection (LCM). About 50 cancer cells in each frozen section were capt ured with the LCM method and processed for RT-PCR detection of EphA2 and Ephrin A-1 mRNA. Results. Among the 206 squamous cervical carcinomas, 23 (11.2%) show ed negative, 94(45.6%) weakly positive, 72 (35.0%)moderately positive,and 17 ( 8.3%) strongly positive for EphA2 immunostaining. For EphrinA-1 protein expres sion, 17 tumors (8.3%) showed negative, 95 (46.1%)weakly positive, 71(34.5%) moderately positive, and 23 (11.2%) strongly positive. EphA2 and EphrinA-1 oft en colocalized in the same tumor areas and vascular endothelial cells. Variable amount of mRNA expressions of EphA2 and EphrinA-1 were observed in the 20 tumor s analyzed. There was no significant correlation between the overexpressions of EphA2/EphrinA-1 and age and FIGO stage. High level of EphA2 was significantly a ssociated with overall survival in univariate and multivariate analysis. Moderat e to high EphrinA-1 protein expression was significantly associated with overal l survival in multivariate analysis. The combined high level of expression of Eh pA2 and moderate to high level of expression of EphrinA-1 was a strong predicto r of overall survival. Conclusions. High levels of EphA2 together with moderate to high level of EphrinA-1 protein expressions in squamous cervical carcinoma w ere predictive for a shorter overall survival and these proteins may be valuable prognostic markers.
