Allograft inflammatory factor-1 （ AIF-1 ） is a cytoplasmic calcium-binding protein involved in inflammatory response-related diseases in mammals. Previously an identified AIF-1 gene was simply reported in yellow gro...Allograft inflammatory factor-1 （ AIF-1 ） is a cytoplasmic calcium-binding protein involved in inflammatory response-related diseases in mammals. Previously an identified AIF-1 gene was simply reported in yellow grouper. The characterization of AIF-1 gene and its expression at the gene and protein level are further described. Yellow grouper AIF-1 is composed of 147 amino acids, and 64% ~ 84% identical to other homologues. Basal level AIF-1 mRNA expression was noted in spleen, anterior kidney and kidney, using reverse-transcription polymerase chain reaction （RT-PCR）. After stimulation of LPS, the AIF-1 mRNA expression was up-regulated in tissues examined： spleen, anterior kidney, kidney, heart and liver, but not in muscle. The recombinant AIF-1 protein was expressed in Escherichia coli, and then purified for the development of antiserum. Western blotting analysis revealed a band with a molecular mass of about 17 ku.展开更多
In order to clarify the mechanism of retinomotor movements in banded grouper, this paper utilizes inhibition factors of microfilaments and microtubules cytochalasin-B and colchicine to study the roles of actin microfi...In order to clarify the mechanism of retinomotor movements in banded grouper, this paper utilizes inhibition factors of microfilaments and microtubules cytochalasin-B and colchicine to study the roles of actin microfilaments and microtubules in the movements of the REP pigment granles and cones. It provides evidences to explain the mechanism of retinomotor movements in fish.展开更多
基金The National Natural Science Foundation of China under contract No. 30671619
文摘Allograft inflammatory factor-1 （ AIF-1 ） is a cytoplasmic calcium-binding protein involved in inflammatory response-related diseases in mammals. Previously an identified AIF-1 gene was simply reported in yellow grouper. The characterization of AIF-1 gene and its expression at the gene and protein level are further described. Yellow grouper AIF-1 is composed of 147 amino acids, and 64% ~ 84% identical to other homologues. Basal level AIF-1 mRNA expression was noted in spleen, anterior kidney and kidney, using reverse-transcription polymerase chain reaction （RT-PCR）. After stimulation of LPS, the AIF-1 mRNA expression was up-regulated in tissues examined： spleen, anterior kidney, kidney, heart and liver, but not in muscle. The recombinant AIF-1 protein was expressed in Escherichia coli, and then purified for the development of antiserum. Western blotting analysis revealed a band with a molecular mass of about 17 ku.
文摘In order to clarify the mechanism of retinomotor movements in banded grouper, this paper utilizes inhibition factors of microfilaments and microtubules cytochalasin-B and colchicine to study the roles of actin microfilaments and microtubules in the movements of the REP pigment granles and cones. It provides evidences to explain the mechanism of retinomotor movements in fish.
