Asperterzine,a symmetric aromatized derivative of epipolythiodioxopiperazine,from the endophytic fungus Aspergillus terreus PR-P-2

A new epipolythiodioxopiperazine （ETP）, asperterzine （1）, along with two known analogs, bisdethiobis （methylthio）-acetylaranotin （2） and bisdethiobis（methylthio）-acetylapoaranotin （3）, was isolated from the plant endophytic fungus Aspergillus terreus PR-P-2. The structure elucidation of 1 was accomplished by a combination of spectral methods and electronic circular dichroism （ECD） spectrum. Asperterzine （1） was a symmetric aromatized ETP found as a natural product for the first time. Compounds 2 and 3 showed strong cytotoxicity against HL-60 cell line. The putative biosynthetic pathway of 1 was also detailed in the text.