AIM： To investigate the positive rate and types of cells that express Epstein-Barr virus-encoded small RNAs （EBERs） and to determine the distribution of EBER-expressing cells in idiopathic orbital inflammatory pseu...AIM： To investigate the positive rate and types of cells that express Epstein-Barr virus-encoded small RNAs （EBERs） and to determine the distribution of EBER-expressing cells in idiopathic orbital inflammatory pseudotumor （IOIP） tissues. METHODS： We retrospectively examined 40 archived paraffin specimens from two teaching hospitals in Southern China between January 2007 and January 2015 that were pathologically determined to exhibit IOIP. Eleven concurrent paraffin specimens of thyroid-associated ophthalmopathy （TAO） composed the control group. In situ hybridization was performed to detect EBERs. Immunohistochemistry was employed to detect CD3, CD20, Vimentin, and smooth muscle actin （SMA）, and the positive rate, types of positive cells, and distribution and location of EBERs were evaluated. RESULTS： The positive expression rate of EBERs was 47.5% （19/40） in the IOIP group, which was significantly higher than that in the TAO group [0 （0/11）, P=-0.011]. in the IOIP group, the lymphocyte infiltrative subtype, fibrotic subtype, and mixed subtype exhibited EBER-positive rates of 57.1% （12121）, 12.5% （118）, and 54.5% （6/11）, respectively, and no significant differences were found between these subtypes （P=0.085）. Positive signals of EBERs were mainly present in medium-small lymphocytes between or around follicles and in the nuclei of activated immunoblasts （14/19）. CONCLUSION： The positive rate, types, and distribution of EBER-expressing cells in IOIP have been documented.These findings are conducive for a better understanding of the underlying mechanisms of Epstein-Barr virus infection in IOIP pathogenesis.展开更多
Background Epstein-Barr virus （EBV） infection is one of the most important factors of nasopharyngeal carcinoma （NPC）endemic areas.Transcription of EBV-encoded non-polyadenylated RNAs （EBERs） are presented in mos...Background Epstein-Barr virus （EBV） infection is one of the most important factors of nasopharyngeal carcinoma （NPC）endemic areas.Transcription of EBV-encoded non-polyadenylated RNAs （EBERs） are presented in most of NPC tumors.Exploring EBERs as a prognostic marker for NPC might further be informative about the biology and the progression of the disease.The aim of this study was to analyze the role of EBV latency in the clinical management of nasopharyngeal carcinoma （NPC）,by detecting EBERs.Methods RNA in situ hybridization （ISH） for detecting EBERs was carried out on 908 NPC tumor tissues.Overall survival （OS） curves were analyzed with the Kaplan-Meier method and the Cox proportional-hazards regression models.Results The median follow-up time was 70 months （1-120 months）.Eight hundred and sixteen （89.9％） from a total of 908 consecutive NPC cases were found to be EBV-EBER positive.EBER-ISH staining revealed nuclear localization in NPC cells.In the Kaplan-Meier analysis for OS,high EBER expression levels in NPC patients were statistically significant positive prognostic factors for survival （log-rank,P=0.022）,especially in adults aged 17-40 years （P=0.023） and in those with advanced stage disease （log-rank,P=0.002）.Cox proportional-hazards regression model analysis showed that the EBER expression level was an independent risk factor for OS （hazard ratio 0.724,P=0.005）.Conclusions EBERs were frequently detected in NPC tumor tissues,and high-level EBER expression correlated with good prognosis in NPC patients,especially in adult patients and in those with advanced stage disease.EBER may serve as a potential prognostic predictor in NPC.展开更多
MicroRNAs(miRNAs) provide insight into both the biology and clinical behavior of many human cancers, including nasopharyngeal carcinoma(NPC). The dysregulation of miRNAs in NPC results in a variety of tumor-promoting ...MicroRNAs(miRNAs) provide insight into both the biology and clinical behavior of many human cancers, including nasopharyngeal carcinoma(NPC). The dysregulation of miRNAs in NPC results in a variety of tumor-promoting effects. Furthermore, several miRNAs are prognostic markers for NPC. In addition to cellular miRNAs, NPC samples also often contain miRNAs encoded by Epstein-Barr virus, and these miRNAs may impact NPC biology by targeting both cellular and viral genes. Given their numerous putative roles in NPC development and progression, a thorough understanding of the impact of miRNA dysregulation in NPC is expected to shed light on useful biomarkers and therapeutic targets for the clinical management of this disease. In this review, we describe the efforts to date to identify and characterize such miRNAs in the context of NPC.展开更多
To look for a more stable and convenient way of constructing short hairpin RNA expression vectors targeting the latent membrane protein-1(LMP-1)encoded by Epstein-Barr virus(pshLMP1),and to study the inhibition functi...To look for a more stable and convenient way of constructing short hairpin RNA expression vectors targeting the latent membrane protein-1(LMP-1)encoded by Epstein-Barr virus(pshLMP1),and to study the inhibition function of pshLMP1 expression vectors in HNE1 cells,we designed the pshLMP1 expression cassette and pshLMP1 expression vectors by both the annealing method and PCR method and then co-transfected with pEGFP-N1-1158 into HNE1 cells to observe the mRNA and protein levels of LMP-1 genes by green fluorescence analysis,RT-PCR and western blot.pshLMP1 expression vectors were successfully obtained by both methods but better cloning efficiency was achieved and fewer deletions and mutations of nucleotides were achieved with the PCR method.Furthermore,the mRNA and protein levels of LMP-1 genes were down-regulated by pshLMP1 expression vectors.According to our research,we found that the PCR method provides a more efficient way to construct pshLMP1 expression vectors which have the ability to inhibit the function of LMP-1 genes expressed in HNE1 cells,and also provides a novel application of RNA interference technology against-EBV.展开更多
基金Supported by the National Natural Science Foundation of China(No.81260149No.81360152+2 种基金No.81560162)Guangxi Natural Science Foundation(No.2016GXNSFAA380301)Youth Science Foundation of Guangxi Medical University(No.GXMUYSF2014040)
文摘AIM： To investigate the positive rate and types of cells that express Epstein-Barr virus-encoded small RNAs （EBERs） and to determine the distribution of EBER-expressing cells in idiopathic orbital inflammatory pseudotumor （IOIP） tissues. METHODS： We retrospectively examined 40 archived paraffin specimens from two teaching hospitals in Southern China between January 2007 and January 2015 that were pathologically determined to exhibit IOIP. Eleven concurrent paraffin specimens of thyroid-associated ophthalmopathy （TAO） composed the control group. In situ hybridization was performed to detect EBERs. Immunohistochemistry was employed to detect CD3, CD20, Vimentin, and smooth muscle actin （SMA）, and the positive rate, types of positive cells, and distribution and location of EBERs were evaluated. RESULTS： The positive expression rate of EBERs was 47.5% （19/40） in the IOIP group, which was significantly higher than that in the TAO group [0 （0/11）, P=-0.011]. in the IOIP group, the lymphocyte infiltrative subtype, fibrotic subtype, and mixed subtype exhibited EBER-positive rates of 57.1% （12121）, 12.5% （118）, and 54.5% （6/11）, respectively, and no significant differences were found between these subtypes （P=0.085）. Positive signals of EBERs were mainly present in medium-small lymphocytes between or around follicles and in the nuclei of activated immunoblasts （14/19）. CONCLUSION： The positive rate, types, and distribution of EBER-expressing cells in IOIP have been documented.These findings are conducive for a better understanding of the underlying mechanisms of Epstein-Barr virus infection in IOIP pathogenesis.
文摘Background Epstein-Barr virus （EBV） infection is one of the most important factors of nasopharyngeal carcinoma （NPC）endemic areas.Transcription of EBV-encoded non-polyadenylated RNAs （EBERs） are presented in most of NPC tumors.Exploring EBERs as a prognostic marker for NPC might further be informative about the biology and the progression of the disease.The aim of this study was to analyze the role of EBV latency in the clinical management of nasopharyngeal carcinoma （NPC）,by detecting EBERs.Methods RNA in situ hybridization （ISH） for detecting EBERs was carried out on 908 NPC tumor tissues.Overall survival （OS） curves were analyzed with the Kaplan-Meier method and the Cox proportional-hazards regression models.Results The median follow-up time was 70 months （1-120 months）.Eight hundred and sixteen （89.9％） from a total of 908 consecutive NPC cases were found to be EBV-EBER positive.EBER-ISH staining revealed nuclear localization in NPC cells.In the Kaplan-Meier analysis for OS,high EBER expression levels in NPC patients were statistically significant positive prognostic factors for survival （log-rank,P=0.022）,especially in adults aged 17-40 years （P=0.023） and in those with advanced stage disease （log-rank,P=0.002）.Cox proportional-hazards regression model analysis showed that the EBER expression level was an independent risk factor for OS （hazard ratio 0.724,P=0.005）.Conclusions EBERs were frequently detected in NPC tumor tissues,and high-level EBER expression correlated with good prognosis in NPC patients,especially in adult patients and in those with advanced stage disease.EBER may serve as a potential prognostic predictor in NPC.
文摘MicroRNAs(miRNAs) provide insight into both the biology and clinical behavior of many human cancers, including nasopharyngeal carcinoma(NPC). The dysregulation of miRNAs in NPC results in a variety of tumor-promoting effects. Furthermore, several miRNAs are prognostic markers for NPC. In addition to cellular miRNAs, NPC samples also often contain miRNAs encoded by Epstein-Barr virus, and these miRNAs may impact NPC biology by targeting both cellular and viral genes. Given their numerous putative roles in NPC development and progression, a thorough understanding of the impact of miRNA dysregulation in NPC is expected to shed light on useful biomarkers and therapeutic targets for the clinical management of this disease. In this review, we describe the efforts to date to identify and characterize such miRNAs in the context of NPC.
文摘To look for a more stable and convenient way of constructing short hairpin RNA expression vectors targeting the latent membrane protein-1(LMP-1)encoded by Epstein-Barr virus(pshLMP1),and to study the inhibition function of pshLMP1 expression vectors in HNE1 cells,we designed the pshLMP1 expression cassette and pshLMP1 expression vectors by both the annealing method and PCR method and then co-transfected with pEGFP-N1-1158 into HNE1 cells to observe the mRNA and protein levels of LMP-1 genes by green fluorescence analysis,RT-PCR and western blot.pshLMP1 expression vectors were successfully obtained by both methods but better cloning efficiency was achieved and fewer deletions and mutations of nucleotides were achieved with the PCR method.Furthermore,the mRNA and protein levels of LMP-1 genes were down-regulated by pshLMP1 expression vectors.According to our research,we found that the PCR method provides a more efficient way to construct pshLMP1 expression vectors which have the ability to inhibit the function of LMP-1 genes expressed in HNE1 cells,and also provides a novel application of RNA interference technology against-EBV.
