Objective:To identify unique immunogenic epitopes of Zika virus non-structural 1(NS1)antigen and produce immunoglobulin Y(IgY)for potential use in he diagnosis of of Zika virus infection.Methods:Immunogenic epitopes w...Objective:To identify unique immunogenic epitopes of Zika virus non-structural 1(NS1)antigen and produce immunoglobulin Y(IgY)for potential use in he diagnosis of of Zika virus infection.Methods:Immunogenic epitopes were identified using in silico B-cell epitope prediction.A synthetic peptide analog of the predicted epitope was used to induce antipeptide IgY production in hens which was purified using affinity chromatography.Presence of purified IgY and its binding specificity were performed by gel electrophoresis and ELISA,respectively.Results:Out of the nine continuous epitopes identified,the sequence at position 193-208(LKVREDYSLECDPAVI)was selected and used to produce anti-peptide IgY.The produced IgY was found to bind to the synthetic analog of the Zika virus NS1 immunogenic epitope but not to other flaviviruses and random peptides from other pathogens.Conclusions:In this study,we identified an immunogenic epitope unique to Zika virus that can be used to develop a serodiagnostic tool that specifically detect Zika virus infection.展开更多
Ether extrilcls of 1693 Chinesc medicinal herbs and plilnts from 268 families werestudied for the induction of Epstcin-Barr viral (EBV ) early antigcn (EA ) expression in theRaji cell line. Fifty-two from 18 families ...Ether extrilcls of 1693 Chinesc medicinal herbs and plilnts from 268 families werestudied for the induction of Epstcin-Barr viral (EBV ) early antigcn (EA ) expression in theRaji cell line. Fifty-two from 18 families were found to have inducing activity. Twenty-fiveand seven of them were from Euphorbiaccae and Thymclaeaceae, respectively. Some ofthem, such as Croton tiglium, Euphorbia kansui, Daphnc genkwa, Wikstrocmia chamacdaphen, Wikstroemia indica, Prunus mandshurica Koehne and Achyranthes bidentata arecommonly used drugs. The significance of these herbs in the activation of EBV in vivo andtheir relation to the development of nasopharyngeal carcinoma were discussed.展开更多
The Epstein-Barr virus membrane antigen was constructed and inserted into vaccinia virus, Tian-tan strain in order to study the effect of this virus on EB infection and tumorogenesis. The EBV-derived membrane antigen ...The Epstein-Barr virus membrane antigen was constructed and inserted into vaccinia virus, Tian-tan strain in order to study the effect of this virus on EB infection and tumorogenesis. The EBV-derived membrane antigen was expressed under the control of a 7.5 K promoter of vaccinia virus. The antibody against the membrane antigen of EB virus was produced on rabbits vaccinated with recombinant vaccinia virus.展开更多
Hepatitis C virus(HCV) is the main cause of chronic liver disease and cirrhosis in Western countries. Over time, the majority of cirrhotic patients develop hepatocellular carcinoma(HCC), one of the most common fatal c...Hepatitis C virus(HCV) is the main cause of chronic liver disease and cirrhosis in Western countries. Over time, the majority of cirrhotic patients develop hepatocellular carcinoma(HCC), one of the most common fatal cancers worldwide- fourth for incidence rate. A high public health priority need is the development of biomarkers to screen for liver disease progression and for early diagnosis of HCC development, particularly in the high risk population represented by HCV-positive patients with cirrhosis. Several studies have shown that serological determination of a novel biomarker, squamous cell carcinoma antigen-immunoglobulins M(SCCA-Ig M), might be useful to identify patients with progressive liver disease. In the initial part of this review we summarize the main clinical studies that have investigated this new circulating biomarker on HCV-infected patients, providing evidence that in chronic hepatitis C SCCA-Ig M may be used to monitor progression of liver disease, and also to assess the virological response to antiviral treatment. In the last part of this review we address other, not less important, clinical applications of this biomarker in hepatology.展开更多
To clarify whether hemorrhagic fever with renal syndrome (HFRS) virus is cytopathic or not for the liver, HFRS virus RNA in liver tissue from 19 patients with HFRS and the livers of naturally infected rats with virus ...To clarify whether hemorrhagic fever with renal syndrome (HFRS) virus is cytopathic or not for the liver, HFRS virus RNA in liver tissue from 19 patients with HFRS and the livers of naturally infected rats with virus were examined by in situ hybridization展开更多
Background Non-invasive nasopharyngeal carcinoma （NPC） screening usually involves serological testing for the presence of IgA antibodies to Epstein-Barr virus （EBV） capsid antigen （VCA）. The present meta-analysi...Background Non-invasive nasopharyngeal carcinoma （NPC） screening usually involves serological testing for the presence of IgA antibodies to Epstein-Barr virus （EBV） capsid antigen （VCA）. The present meta-analysis determined the accuracy of VCA-lgA in the diagnosis of NPC.Methods A systematic review of studies was conducted and data on the accuracy of VCA-lgA concentrations in the diagnosis of NPC were pooled using random effects models. Receiver operating characteristic curves were used to summarize the overall test performance. Results Twenty studies met the inclusion criteria for the meta-analysis. The summary estimates for VCA-lgA in the diagnosis of NPC were： sensitivity 0.91 （95% confidence interval （Cl）： 0.90-0.92）, specificity 0.92 （95% Cl： 0.92-0.93）, positive likelihood ratio 31.65 （95% Cl： 10.99-91.15）, negative likelihood ratio 0.10 （95% Cl： 0.07-0.13） and diagnostic odds ratio 414.59 （95% Cl： 174.96-982.42）. The area under the summary receiver operating characteristic curves was 0.98.Conclusion The sensitivity and the specificity of serum VCA-lgA are very high, suggesting that the presence of VCA-lgA in peripheral blood is a valuable predictor for NPC.展开更多
Approximately 240 million people are chronically infected with hepatitis B. The implementation of rigorous vaccination programs has led to an overall decrease in the prevalence of this disease worldwide but this may a...Approximately 240 million people are chronically infected with hepatitis B. The implementation of rigorous vaccination programs has led to an overall decrease in the prevalence of this disease worldwide but this may also have led to emergence of viral mutations that can escape the protection of hepatitis B surface antibody. As this phenomenon is increasingly recognized, concern for transmission to vaccinated individuals has also been raised. Herein, we describe two cases where the suspected presence of a hepatitis B surface antigen escape mutation impacted the decision to initiate early antiviral therapy, as well as provide a brief review of these mutations. Our findings described here suggest that a lower threshold for initiating therapy in these individuals should be considered in order to reduce the risk of transmission, as vaccination does not provide protection.展开更多
Background Intestinal T-cell lymphoma （ITCL） is a heterogeneous lymphoid neoplastic group with variable clinical and pathological features. ITCL in oriental countries is different from enteropathy-type intestinal T-...Background Intestinal T-cell lymphoma （ITCL） is a heterogeneous lymphoid neoplastic group with variable clinical and pathological features. ITCL in oriental countries is different from enteropathy-type intestinal T-cell lymphoma （ETCL） in relation to celiac disease and Epstein-Barr virus （EBV）. The objective of this study was to investigate the clinicopathological features, immunophenotype, expression of cytotoxic molecule （TIA-1）, T-cell receptor （TCR）-γ gene rearrangement, and Epstein-Barr virus （EBV） latent infection in primary ITCL without celiac disease in Chinese. Methods The clinical data of 42 patients were analyzed, and the patients were followed up. Compared with human reactive lymphoid tissues, in situ hybridization for EBER1/2, polymerase chain reaction for TCR-~/gene rearrangement, and immunohistochemical staining for immunophenotypes, TIA-1 and EBV latent membrane proteins （LMP-1） were investigated. Survival curves of different clinicopathological features, immunophenotypes, expression of LMPI , TCR-γ/gene rearrangement and therapy were analyzed. Results Three fourths of the patients suffered from ITCL in China were men with a peak age incidence in the 4th decade. Common presenting features included fever and hemotochezia. The prognosis was poor with a median survival of 3.0 months. The lesions were mostly localized in the ileocecum and colon. About 38/42 （90. 5% ） patients demonstrated pleomorphic medium-sized on large seen. All 42 patients with ITCL revealed CD45RO cells. Histological features of celiac disease were rarely positive. Neoplastic cells partially expressed T-cell differentiated antigens （CD3ε, CD4, CD8） and NK cell associated antigen （CD56）. The positive frequency of CD3e, CIM, CD8 and CD56 was 28/42 （66.7%） 7/42 （16.7%）, 10/42 （23.8%） and 12/42 （28.6%） respectively. Thirty-nine cells （92. 9% ） expressed TIA-1, but none expressed CD20 and CD68. More than half of the patients （64. 3% , 64.3% and 59.5% ） revealed TCR-γ primers respectively. EBER1/2 was detected in 41 （97.6%） of LMP-1 was 38. 1% （16/42）. gene rearrangement by three different TCR-γ the 42 patients. The expression frequency ofConclusions Primary ITCL without celiac disease in Chinese is a special highly EBV-associated clinicopathological entity. There are few similarities in patients with celiac disease in western countries. A small proportion of primary ITCLs in Chinese and extranodal NK/T-eell lymphoma of nasal type belong to the same speetrum.展开更多
The peptide of amino acids 141～160 of VP1 protein of foot\|and\|mouth disease virus (FMDV) is a major B cell epitope and the peptide of amino acids 21～40 is an important T cell epitope.In this study,the DNA fragment...The peptide of amino acids 141～160 of VP1 protein of foot\|and\|mouth disease virus (FMDV) is a major B cell epitope and the peptide of amino acids 21～40 is an important T cell epitope.In this study,the DNA fragments of 141～160 and 21～40 peptide epitopes of a strain of type O FMDV was chemically synthesized and arranged into a tandem repeat 141～160 (20AA)\|21～40 (20AA)\|141～160 (20AA).This tandem sequence was fused to the 3′ end of the heavy chain constant region gene of swine immunoglobulin G and was then cloned into mammalian expression vector pCDM8 to form a recombinant plasmid pCDM8FZ3.After pCDM8FZ3 was inoculated intramuscularly into guinea pigs,it elicited a neutralizing antibody response and a specific spleen T cell proliferative response,and 66% of the vaccinated animals were protected from viral challenge.Our study indicated that the heavy chain constant region of swine IgG can act as the carrier protein for FMDV peptide epitopes,and pCDM8FZ3 is a potential DNA vaccine candidate to prevent FMDV infection.展开更多
目的探究EB病毒抗原免疫球蛋白A(EB-IgA)联合内径窄带成像术(narrow band imaging,NBI)在鼻咽癌筛查中的临床应用价值。方法选取2020年12月—2022年12月于河池市人民医院就诊的以涕中带血、耳闷堵感、听力下降等为主诉的521例患者为研...目的探究EB病毒抗原免疫球蛋白A(EB-IgA)联合内径窄带成像术(narrow band imaging,NBI)在鼻咽癌筛查中的临床应用价值。方法选取2020年12月—2022年12月于河池市人民医院就诊的以涕中带血、耳闷堵感、听力下降等为主诉的521例患者为研究对象。分别对其开展EB-IgA及NBI内镜检查,以病理检查结果为参照标准。比较EB-IgA、NBI和EB-IgA+NBI三种技术对鼻咽癌筛查价值的差异。结果EB-IgA筛选出鼻咽癌患者61例、非鼻咽癌患者236例;灵敏度为70.11%、特异度为54.38%、准确度为57.01%、阳性预测值为23.55%。NBI筛选出鼻咽癌患者72例、非鼻咽癌患者362例;灵敏度为82.76%、特异度为83.41%、准确度为83.30%、阳性预测值为50.00%。EB-IgA+NBI筛选出鼻咽癌患者83例、非鼻咽癌患者405例,灵敏度为95.40%、特异度为93.32%、准确度为93.67%、阳性预测值为74.11%。EB-IgA+NBI鼻咽癌诊断的灵敏度、特异度、准确度、阳性预测值高于NBI及EB-IgA,差异有统计学意义(P<0.05)。结论NBI有助于更好地观察到黏膜表面细微结构的变化,其在鼻咽癌筛查中的诊断价值要优于EB-IgA,但如能够将EB-IgA同NBI检测联合起来,则可以明显提高鼻咽癌筛查的准确率,建议临床上选择两种方式联合筛查作为鼻咽癌初筛手段。展开更多
文摘Objective:To identify unique immunogenic epitopes of Zika virus non-structural 1(NS1)antigen and produce immunoglobulin Y(IgY)for potential use in he diagnosis of of Zika virus infection.Methods:Immunogenic epitopes were identified using in silico B-cell epitope prediction.A synthetic peptide analog of the predicted epitope was used to induce antipeptide IgY production in hens which was purified using affinity chromatography.Presence of purified IgY and its binding specificity were performed by gel electrophoresis and ELISA,respectively.Results:Out of the nine continuous epitopes identified,the sequence at position 193-208(LKVREDYSLECDPAVI)was selected and used to produce anti-peptide IgY.The produced IgY was found to bind to the synthetic analog of the Zika virus NS1 immunogenic epitope but not to other flaviviruses and random peptides from other pathogens.Conclusions:In this study,we identified an immunogenic epitope unique to Zika virus that can be used to develop a serodiagnostic tool that specifically detect Zika virus infection.
文摘Ether extrilcls of 1693 Chinesc medicinal herbs and plilnts from 268 families werestudied for the induction of Epstcin-Barr viral (EBV ) early antigcn (EA ) expression in theRaji cell line. Fifty-two from 18 families were found to have inducing activity. Twenty-fiveand seven of them were from Euphorbiaccae and Thymclaeaceae, respectively. Some ofthem, such as Croton tiglium, Euphorbia kansui, Daphnc genkwa, Wikstrocmia chamacdaphen, Wikstroemia indica, Prunus mandshurica Koehne and Achyranthes bidentata arecommonly used drugs. The significance of these herbs in the activation of EBV in vivo andtheir relation to the development of nasopharyngeal carcinoma were discussed.
文摘The Epstein-Barr virus membrane antigen was constructed and inserted into vaccinia virus, Tian-tan strain in order to study the effect of this virus on EB infection and tumorogenesis. The EBV-derived membrane antigen was expressed under the control of a 7.5 K promoter of vaccinia virus. The antibody against the membrane antigen of EB virus was produced on rabbits vaccinated with recombinant vaccinia virus.
文摘Hepatitis C virus(HCV) is the main cause of chronic liver disease and cirrhosis in Western countries. Over time, the majority of cirrhotic patients develop hepatocellular carcinoma(HCC), one of the most common fatal cancers worldwide- fourth for incidence rate. A high public health priority need is the development of biomarkers to screen for liver disease progression and for early diagnosis of HCC development, particularly in the high risk population represented by HCV-positive patients with cirrhosis. Several studies have shown that serological determination of a novel biomarker, squamous cell carcinoma antigen-immunoglobulins M(SCCA-Ig M), might be useful to identify patients with progressive liver disease. In the initial part of this review we summarize the main clinical studies that have investigated this new circulating biomarker on HCV-infected patients, providing evidence that in chronic hepatitis C SCCA-Ig M may be used to monitor progression of liver disease, and also to assess the virological response to antiviral treatment. In the last part of this review we address other, not less important, clinical applications of this biomarker in hepatology.
文摘To clarify whether hemorrhagic fever with renal syndrome (HFRS) virus is cytopathic or not for the liver, HFRS virus RNA in liver tissue from 19 patients with HFRS and the livers of naturally infected rats with virus were examined by in situ hybridization
文摘Background Non-invasive nasopharyngeal carcinoma （NPC） screening usually involves serological testing for the presence of IgA antibodies to Epstein-Barr virus （EBV） capsid antigen （VCA）. The present meta-analysis determined the accuracy of VCA-lgA in the diagnosis of NPC.Methods A systematic review of studies was conducted and data on the accuracy of VCA-lgA concentrations in the diagnosis of NPC were pooled using random effects models. Receiver operating characteristic curves were used to summarize the overall test performance. Results Twenty studies met the inclusion criteria for the meta-analysis. The summary estimates for VCA-lgA in the diagnosis of NPC were： sensitivity 0.91 （95% confidence interval （Cl）： 0.90-0.92）, specificity 0.92 （95% Cl： 0.92-0.93）, positive likelihood ratio 31.65 （95% Cl： 10.99-91.15）, negative likelihood ratio 0.10 （95% Cl： 0.07-0.13） and diagnostic odds ratio 414.59 （95% Cl： 174.96-982.42）. The area under the summary receiver operating characteristic curves was 0.98.Conclusion The sensitivity and the specificity of serum VCA-lgA are very high, suggesting that the presence of VCA-lgA in peripheral blood is a valuable predictor for NPC.
文摘Approximately 240 million people are chronically infected with hepatitis B. The implementation of rigorous vaccination programs has led to an overall decrease in the prevalence of this disease worldwide but this may also have led to emergence of viral mutations that can escape the protection of hepatitis B surface antibody. As this phenomenon is increasingly recognized, concern for transmission to vaccinated individuals has also been raised. Herein, we describe two cases where the suspected presence of a hepatitis B surface antigen escape mutation impacted the decision to initiate early antiviral therapy, as well as provide a brief review of these mutations. Our findings described here suggest that a lower threshold for initiating therapy in these individuals should be considered in order to reduce the risk of transmission, as vaccination does not provide protection.
文摘Background Intestinal T-cell lymphoma （ITCL） is a heterogeneous lymphoid neoplastic group with variable clinical and pathological features. ITCL in oriental countries is different from enteropathy-type intestinal T-cell lymphoma （ETCL） in relation to celiac disease and Epstein-Barr virus （EBV）. The objective of this study was to investigate the clinicopathological features, immunophenotype, expression of cytotoxic molecule （TIA-1）, T-cell receptor （TCR）-γ gene rearrangement, and Epstein-Barr virus （EBV） latent infection in primary ITCL without celiac disease in Chinese. Methods The clinical data of 42 patients were analyzed, and the patients were followed up. Compared with human reactive lymphoid tissues, in situ hybridization for EBER1/2, polymerase chain reaction for TCR-~/gene rearrangement, and immunohistochemical staining for immunophenotypes, TIA-1 and EBV latent membrane proteins （LMP-1） were investigated. Survival curves of different clinicopathological features, immunophenotypes, expression of LMPI , TCR-γ/gene rearrangement and therapy were analyzed. Results Three fourths of the patients suffered from ITCL in China were men with a peak age incidence in the 4th decade. Common presenting features included fever and hemotochezia. The prognosis was poor with a median survival of 3.0 months. The lesions were mostly localized in the ileocecum and colon. About 38/42 （90. 5% ） patients demonstrated pleomorphic medium-sized on large seen. All 42 patients with ITCL revealed CD45RO cells. Histological features of celiac disease were rarely positive. Neoplastic cells partially expressed T-cell differentiated antigens （CD3ε, CD4, CD8） and NK cell associated antigen （CD56）. The positive frequency of CD3e, CIM, CD8 and CD56 was 28/42 （66.7%） 7/42 （16.7%）, 10/42 （23.8%） and 12/42 （28.6%） respectively. Thirty-nine cells （92. 9% ） expressed TIA-1, but none expressed CD20 and CD68. More than half of the patients （64. 3% , 64.3% and 59.5% ） revealed TCR-γ primers respectively. EBER1/2 was detected in 41 （97.6%） of LMP-1 was 38. 1% （16/42）. gene rearrangement by three different TCR-γ the 42 patients. The expression frequency ofConclusions Primary ITCL without celiac disease in Chinese is a special highly EBV-associated clinicopathological entity. There are few similarities in patients with celiac disease in western countries. A small proportion of primary ITCLs in Chinese and extranodal NK/T-eell lymphoma of nasal type belong to the same speetrum.
文摘The peptide of amino acids 141～160 of VP1 protein of foot\|and\|mouth disease virus (FMDV) is a major B cell epitope and the peptide of amino acids 21～40 is an important T cell epitope.In this study,the DNA fragments of 141～160 and 21～40 peptide epitopes of a strain of type O FMDV was chemically synthesized and arranged into a tandem repeat 141～160 (20AA)\|21～40 (20AA)\|141～160 (20AA).This tandem sequence was fused to the 3′ end of the heavy chain constant region gene of swine immunoglobulin G and was then cloned into mammalian expression vector pCDM8 to form a recombinant plasmid pCDM8FZ3.After pCDM8FZ3 was inoculated intramuscularly into guinea pigs,it elicited a neutralizing antibody response and a specific spleen T cell proliferative response,and 66% of the vaccinated animals were protected from viral challenge.Our study indicated that the heavy chain constant region of swine IgG can act as the carrier protein for FMDV peptide epitopes,and pCDM8FZ3 is a potential DNA vaccine candidate to prevent FMDV infection.
文摘目的探究EB病毒抗原免疫球蛋白A(EB-IgA)联合内径窄带成像术(narrow band imaging,NBI)在鼻咽癌筛查中的临床应用价值。方法选取2020年12月—2022年12月于河池市人民医院就诊的以涕中带血、耳闷堵感、听力下降等为主诉的521例患者为研究对象。分别对其开展EB-IgA及NBI内镜检查,以病理检查结果为参照标准。比较EB-IgA、NBI和EB-IgA+NBI三种技术对鼻咽癌筛查价值的差异。结果EB-IgA筛选出鼻咽癌患者61例、非鼻咽癌患者236例;灵敏度为70.11%、特异度为54.38%、准确度为57.01%、阳性预测值为23.55%。NBI筛选出鼻咽癌患者72例、非鼻咽癌患者362例;灵敏度为82.76%、特异度为83.41%、准确度为83.30%、阳性预测值为50.00%。EB-IgA+NBI筛选出鼻咽癌患者83例、非鼻咽癌患者405例,灵敏度为95.40%、特异度为93.32%、准确度为93.67%、阳性预测值为74.11%。EB-IgA+NBI鼻咽癌诊断的灵敏度、特异度、准确度、阳性预测值高于NBI及EB-IgA,差异有统计学意义(P<0.05)。结论NBI有助于更好地观察到黏膜表面细微结构的变化,其在鼻咽癌筛查中的诊断价值要优于EB-IgA,但如能够将EB-IgA同NBI检测联合起来,则可以明显提高鼻咽癌筛查的准确率,建议临床上选择两种方式联合筛查作为鼻咽癌初筛手段。
