In this study, twelve primers were used for ISSR （ inter simple sequence repeat） analysis of eight F1 hybrids of Erianthu.sfulvus and sugarcane （ Saccha- rum spp. ）. The results showed that totally 133 bands were ...In this study, twelve primers were used for ISSR （ inter simple sequence repeat） analysis of eight F1 hybrids of Erianthu.sfulvus and sugarcane （ Saccha- rum spp. ）. The results showed that totally 133 bands were amplified, including 111 polymorphic bands, showing a polymorphic rate of 83.46%, which indicated that there are abundant genetic variations among F1 progenies derived from crossing between cultivated sugarcane and E. fu/vus. The average genetic similarity coef- ficients between F1 progenies and female parent Yacheng 89-9, F1 progenies and male parent E. fidvas were 0.67 and 0.44, respectively. UPGMA clustering result of F1 progenies showed that all the progenies were clustered with female parent, suggesting that the genetic material of female parent was predominated in F： proge- nies, which also revealed that the chromosome number of F, progenies was not directly related to the clustering results based on ISSR analysis.展开更多
In this study, karyotype analysis of F2BCl progenies between sugarcane （Saccharum spp. ） and Erianthusfulvus was conducted. The result showed that most chromosomes of YAU04/14, YAU09/02, YAU09/05, YAU09/26 and YAU09...In this study, karyotype analysis of F2BCl progenies between sugarcane （Saccharum spp. ） and Erianthusfulvus was conducted. The result showed that most chromosomes of YAU04/14, YAU09/02, YAU09/05, YAU09/26 and YAU09/52 are median region （m） chromosomes, while only a small amount of chromosomes are submedian （sin） chromosomes and median point （M） chromosomes. The karyotype formulae of five experimental materials are 2n = 106 = 98m ＋ 8sm, 2n = 106 - 2M ＋ 104m, 2n = 106 = 4M ＋ 96m ＋ 6sin, 2n = 102 = 2M ＋ 92m ＋ 8sin and 2n = 106 = 4M ＋ 96m ＋ 6sin, respectively. The karyotypes of all materials belong to symmetrical 2B type, indicating that the chromosomes of progenies are primitive.展开更多
Erianthus produces substantial biomass,exhibits a good Brix value,and shows wide environmental adaptability,making it a potential biofuel plant.In contrast to closely related sorghum and sugarcane,Erianthus can grow i...Erianthus produces substantial biomass,exhibits a good Brix value,and shows wide environmental adaptability,making it a potential biofuel plant.In contrast to closely related sorghum and sugarcane,Erianthus can grow in degraded soils,thus releasing pressure on agricultural lands used for biofuel production.However,the lack of genomic resources for Erianthus hinders its genetic improvement,thus limiting its potential for biofuel production.In the present study,we generated a chromosome-scale reference genome for Erianthus fulvus Nees.The genome size estimated by flow cytometry was 937 Mb,and the assembled genome size was 902 Mb,covering 96.26%of the estimated genome size.A total of 35065 proteincoding genes were predicted,and 67.89%of the genome was found to be repetitive.A recent wholegenome duplication occurred approximately 74.10 million years ago in the E.fulvus genome.Phylogenetic analysis showed that E.fulvus is evolutionarily closer to S.spontaneum and diverged after S.bicolor.Three of the 10 chromosomes of E.fulvus formed through rearrangements of ancestral chromosomes.Phylogenetic reconstruction of the Saccharum complex revealed a polyphyletic origin of the complex and a sister relationship of E.fulvus with Saccharum sp.,excluding S.arundinaceum.On the basis of the four amino acid residues that provide substrate specificity,the E.fulvus SWEET proteins were classified as monoand disaccharide sugar transporters.Ortho-QTL genes identified for 10 biofuel-related traits may aid in the rapid screening of E.fulvus populations to enhance breeding programs for improved biofuel production.The results of this study provide valuable insights for breeding programs aimed at improving biofuel production in E.fulvus and enhancing sugarcane introgression programs.展开更多
基金Supported by Key New Product Development Project of Yunnan Province(2010BB011)National Natural Science Foundation of China (30960045)+2 种基金Yunnan Agricultural Science and Technology Key Project during the Eleventh Five-Year Plan (2006NG11)Talent Training Project of Yunnan Province(2006PY-38)Yunnan Provincial Modern Agricultural Industry Technology System Construction Project of Sugarcane Breeding Research Center
文摘In this study, twelve primers were used for ISSR （ inter simple sequence repeat） analysis of eight F1 hybrids of Erianthu.sfulvus and sugarcane （ Saccha- rum spp. ）. The results showed that totally 133 bands were amplified, including 111 polymorphic bands, showing a polymorphic rate of 83.46%, which indicated that there are abundant genetic variations among F1 progenies derived from crossing between cultivated sugarcane and E. fu/vus. The average genetic similarity coef- ficients between F1 progenies and female parent Yacheng 89-9, F1 progenies and male parent E. fidvas were 0.67 and 0.44, respectively. UPGMA clustering result of F1 progenies showed that all the progenies were clustered with female parent, suggesting that the genetic material of female parent was predominated in F： proge- nies, which also revealed that the chromosome number of F, progenies was not directly related to the clustering results based on ISSR analysis.
基金Supported by National Natural Science Foundation of China(30960045)Program for Construction of Modern Agricultural Sugarcane Industry Technology System of Yunnan Province(YCJ[2014]No.105)+3 种基金Key Project of New Product Development of Yunnan Province(Agriculture,2012BB014)Innovation Team Project for Sustainable Production System of Mountain Plateau Crops of Yunnan Agricultural University(YKRF[2012]No.18)Research Fund for the Doctoral Program of Yunnan Agricultural University(A2002181)General Program of Applied Basic Research of Yunnan Province(2013FB037)
文摘In this study, karyotype analysis of F2BCl progenies between sugarcane （Saccharum spp. ） and Erianthusfulvus was conducted. The result showed that most chromosomes of YAU04/14, YAU09/02, YAU09/05, YAU09/26 and YAU09/52 are median region （m） chromosomes, while only a small amount of chromosomes are submedian （sin） chromosomes and median point （M） chromosomes. The karyotype formulae of five experimental materials are 2n = 106 = 98m ＋ 8sm, 2n = 106 - 2M ＋ 104m, 2n = 106 = 4M ＋ 96m ＋ 6sin, 2n = 102 = 2M ＋ 92m ＋ 8sin and 2n = 106 = 4M ＋ 96m ＋ 6sin, respectively. The karyotypes of all materials belong to symmetrical 2B type, indicating that the chromosomes of progenies are primitive.
基金supported by grants from the Major Science and Technology Projects in Yunnan Province(202202AE090021)a special project of Yunnan Key Laboratory of Crop Production and Smart Agriculture(202105AG070007)+3 种基金a sub-project of the National Key Research and Development Program of China(2018YFD1000503)the National Natural Science Foundation of China(31960451,31560417)a Key Project of Applied Basic Research Program of Yunnan Province(2015FA024)the ESI Discipline Promotion Program of Yunnan Agricultural University(2019YNAUESIMS01).
文摘Erianthus produces substantial biomass,exhibits a good Brix value,and shows wide environmental adaptability,making it a potential biofuel plant.In contrast to closely related sorghum and sugarcane,Erianthus can grow in degraded soils,thus releasing pressure on agricultural lands used for biofuel production.However,the lack of genomic resources for Erianthus hinders its genetic improvement,thus limiting its potential for biofuel production.In the present study,we generated a chromosome-scale reference genome for Erianthus fulvus Nees.The genome size estimated by flow cytometry was 937 Mb,and the assembled genome size was 902 Mb,covering 96.26%of the estimated genome size.A total of 35065 proteincoding genes were predicted,and 67.89%of the genome was found to be repetitive.A recent wholegenome duplication occurred approximately 74.10 million years ago in the E.fulvus genome.Phylogenetic analysis showed that E.fulvus is evolutionarily closer to S.spontaneum and diverged after S.bicolor.Three of the 10 chromosomes of E.fulvus formed through rearrangements of ancestral chromosomes.Phylogenetic reconstruction of the Saccharum complex revealed a polyphyletic origin of the complex and a sister relationship of E.fulvus with Saccharum sp.,excluding S.arundinaceum.On the basis of the four amino acid residues that provide substrate specificity,the E.fulvus SWEET proteins were classified as monoand disaccharide sugar transporters.Ortho-QTL genes identified for 10 biofuel-related traits may aid in the rapid screening of E.fulvus populations to enhance breeding programs for improved biofuel production.The results of this study provide valuable insights for breeding programs aimed at improving biofuel production in E.fulvus and enhancing sugarcane introgression programs.
