The effect of lanthanum on the growth and extracellular enzyme activity of Erwinia chrysanthemi(Ech) was studied. Lanthanum inhibited the growth of Ech in solid culture at the concentration of 50, 100, 150, 200, 250, ...The effect of lanthanum on the growth and extracellular enzyme activity of Erwinia chrysanthemi(Ech) was studied. Lanthanum inhibited the growth of Ech in solid culture at the concentration of 50, 100, 150, 200, 250, 300 and 350 mgL-1, and the inhibition increased with the increase of the RE concentration. The formation of colony delayed and the diameter of colony decreased. Lanthanum stimulated the growth of Ech within 24h when the lanthanum concentration was less than 200 mgL-1 in liquid medium, but significantly inhibited the growth of Ech with the increase of lanthanum concentration and culture time. When the concentration was higher than 350 mgL-1, the growth of Ech was inhibited completely both in solid and liquid culture. The extracellular enzyme acitivity of Ech was affected by lanthanum at 200 mgL-1. The activity of cellulose was promoted more than that of protease but the activity of pectinase was decreased. The maceration to potato tuber tissue decreased when the cell free filtrate was tr展开更多
为了明确引起云南香蕉细菌性软腐病的病原菌,为该病害防控提供基础,在植蕉区采集香蕉细菌性软腐病病害标样,对病原菌进行分离、致病性测定、寄主范围测定、形态学观察、生理生化反应及16S r DNA序列分析。结果表明:分离获得的菌株均有...为了明确引起云南香蕉细菌性软腐病的病原菌,为该病害防控提供基础,在植蕉区采集香蕉细菌性软腐病病害标样,对病原菌进行分离、致病性测定、寄主范围测定、形态学观察、生理生化反应及16S r DNA序列分析。结果表明:分离获得的菌株均有致病性,病原菌形态特征、寄主范围、生理生化测定结果与Erwinia chrysanthemi相符。16S r DNA序列分析表明:代表性菌株的测序结果与E.chrysanthemi(序列登录号:GU811708)的同源性达到99%以上,系统发育树分析结果与之一致,表明引起云南香蕉细菌性软腐病的病原菌为菊欧氏杆菌(E.chrysanthemi),该病害是近年来发生在云南植蕉区的一种新病害。展开更多
文摘The effect of lanthanum on the growth and extracellular enzyme activity of Erwinia chrysanthemi(Ech) was studied. Lanthanum inhibited the growth of Ech in solid culture at the concentration of 50, 100, 150, 200, 250, 300 and 350 mgL-1, and the inhibition increased with the increase of the RE concentration. The formation of colony delayed and the diameter of colony decreased. Lanthanum stimulated the growth of Ech within 24h when the lanthanum concentration was less than 200 mgL-1 in liquid medium, but significantly inhibited the growth of Ech with the increase of lanthanum concentration and culture time. When the concentration was higher than 350 mgL-1, the growth of Ech was inhibited completely both in solid and liquid culture. The extracellular enzyme acitivity of Ech was affected by lanthanum at 200 mgL-1. The activity of cellulose was promoted more than that of protease but the activity of pectinase was decreased. The maceration to potato tuber tissue decreased when the cell free filtrate was tr
文摘为了明确引起云南香蕉细菌性软腐病的病原菌,为该病害防控提供基础,在植蕉区采集香蕉细菌性软腐病病害标样,对病原菌进行分离、致病性测定、寄主范围测定、形态学观察、生理生化反应及16S r DNA序列分析。结果表明:分离获得的菌株均有致病性,病原菌形态特征、寄主范围、生理生化测定结果与Erwinia chrysanthemi相符。16S r DNA序列分析表明:代表性菌株的测序结果与E.chrysanthemi(序列登录号:GU811708)的同源性达到99%以上,系统发育树分析结果与之一致,表明引起云南香蕉细菌性软腐病的病原菌为菊欧氏杆菌(E.chrysanthemi),该病害是近年来发生在云南植蕉区的一种新病害。
