Spermatogenesis is a complicated and highly regulated male gamete differentiation process that begins with the proliferation and differentiation of spermatogonia to the release of the mature spermatozoa.The autophagy-...Spermatogenesis is a complicated and highly regulated male gamete differentiation process that begins with the proliferation and differentiation of spermatogonia to the release of the mature spermatozoa.The autophagy-related gene atg7 has been reported as closely related to spermatogenesis and communication of Sertoli cell-germ cells in mice,including acrosome biogenesis,sperm flagellum development,and ectoplasmic specialization assembly.However,the function of es-ATG7 and its molecular regulatory mechanism during spermatogenesis in Crustacea remain largely unknown.Here,we cloned and identified es-atg7 from the testes of the Chinese mitten crabs Eriocheir sinensis and found that the expression of es-atg7 was relatively high in testes through semi-quantitative RT-PCR.The dynamic localization of es-ATG7 detected by immunofluorescence may convey information about its role in the spermatogenesis of E.sinensis.Furthermore,a knockdown of es-atg7 revealed that the malformed sperm with irregular sperm shape or loose nuclear cup and germ cell apoptosis were increased significantly.Accompanying this,we found an up-regulated expression of es-p53 during spermatogenesis in es-atg7 knockdown groups.Altogether,our results indicate that es-ATG7 plays an essential role during spermatogenesis of E.sinensis,and we demonstrated that es-ATG7 acts as an antagonist for p53-dependent apoptosis induction in this process.展开更多
基金supported by the National Natural Science Foundation of China(No.32072954 and No.41776144)Zhejiang Province Public Welfare Technology Application Research Project(including Natural Science Foundation)(No.LGF20C120001).
文摘Spermatogenesis is a complicated and highly regulated male gamete differentiation process that begins with the proliferation and differentiation of spermatogonia to the release of the mature spermatozoa.The autophagy-related gene atg7 has been reported as closely related to spermatogenesis and communication of Sertoli cell-germ cells in mice,including acrosome biogenesis,sperm flagellum development,and ectoplasmic specialization assembly.However,the function of es-ATG7 and its molecular regulatory mechanism during spermatogenesis in Crustacea remain largely unknown.Here,we cloned and identified es-atg7 from the testes of the Chinese mitten crabs Eriocheir sinensis and found that the expression of es-atg7 was relatively high in testes through semi-quantitative RT-PCR.The dynamic localization of es-ATG7 detected by immunofluorescence may convey information about its role in the spermatogenesis of E.sinensis.Furthermore,a knockdown of es-atg7 revealed that the malformed sperm with irregular sperm shape or loose nuclear cup and germ cell apoptosis were increased significantly.Accompanying this,we found an up-regulated expression of es-p53 during spermatogenesis in es-atg7 knockdown groups.Altogether,our results indicate that es-ATG7 plays an essential role during spermatogenesis of E.sinensis,and we demonstrated that es-ATG7 acts as an antagonist for p53-dependent apoptosis induction in this process.
