Objective: To determine the occurrence of CTX-M producing Escherichia coli(E. coli)from cattle feces in Bogor slaughterhouse, Indonesia.Methods: A total of 220 cattle feces samples were collected from Bogor slaughterh...Objective: To determine the occurrence of CTX-M producing Escherichia coli(E. coli)from cattle feces in Bogor slaughterhouse, Indonesia.Methods: A total of 220 cattle feces samples were collected from Bogor slaughterhouse from March to April 2015. Presence of extended-spectrum beta-lactamase(ESBL) producing E. coli was detected by disc diffusion test based on the recommendation from Clinical and Laboratory Standards Institute(2014). Bacterial strains which were confirmed as producing ESBLs were further analyzed for the presence of bla genes of the ESBL by PCR.Results: The results showed that CTX-M producing E. coli isolates were detected in 19 samples from 220 samples(8.6%). The b-lactamase genes detected were CTX-M-1(n = 10) and CTX-M-9(n = 9). All of the CTX-M producing E. coli isolates showed multidrug resistance phenotypes to at least four antibiotics. The highest incidence of antibiotics resistance was showed to ampicillin(100.0%), cefotaxime(100.0%), and cefpodoxime(100.0%), followed by streptomycin(84.3%), trimethoprim-sulfamethoxazole(73.7%), erythromycin(52.6%), kanamycin(26.3%), doxycycline(10.5%), and ceftazidime(0.0%).Conclusions: Detection of CTX-M-producing E. coli in cattle feces raises important questions as they can represent a potential risk factor to public health.展开更多
This study was conducted to investigate the drug sensitivity of Escherichia coli isolated from sheep, providing data reference for clinical medication. Fifty-four samples were collected from a certain sheep farm in Ya...This study was conducted to investigate the drug sensitivity of Escherichia coli isolated from sheep, providing data reference for clinical medication. Fifty-four samples were collected from a certain sheep farm in Yangling, Shaanxi Province for E. coli isolation and identification, and K-B disk diffusion method was used to carry out the dug sensitivity test of 40 E. coli to the eight antimicrobials of spectinomycin, amikacin, tobramycin, enrofloxacin, nalidixic acid, levofloxacin, salafloxacin and ciprofloxacin. The results showed that there were 40 positive E. coli strains and the isolation rate was 74.7%. The resistance rates of the 40 E. coli to spectinomycin, amikacin,tobramycin and nalidixic acid were 85%, 95%, 97.5% and 87.5%, respectively, while the resistance rate to enrofloxacin, levofloxacin, salafloxacin and ciprofloxacin were relatively low and the resistance rates were 35%, 20%, 25% and 17.5%, respectively. The isolates showed different levels of drug resistance to eight antimicrobials and the highest resistance rate was 97.5%. It reminds that the sheep farm should strictly control the dosage according to the antimicrobial sensitive data in clinic.展开更多
[ Objective] In order to provide a reference for prevention and control of contagious pleuropneumonia and E. coli mixed infection. [ Meth- od] A comprehensive introduction of the two diseases was given on the followin...[ Objective] In order to provide a reference for prevention and control of contagious pleuropneumonia and E. coli mixed infection. [ Meth- od] A comprehensive introduction of the two diseases was given on the following aspects： morbidity, clinical symptoms, pathological changes, la- boratory diagnosis, treatment, prevention, and prevention and control measures. [ Results] Sound development of sheep production was seriously influenced by the two diseases, effective prevention and control measures must be adopted to inhibit the occurrence and prevalence of sheep conta- gious pleuropneumonia and colibacillosis. [ Conclusion] The two epidemic diseases were controllable.展开更多
The use of antibiotics for prophylaxis and growth enhancement in livestock farming is on the increase globally. This practice has led to the emergence and spread of antimicrobial-resistant bacteria in livestock. Only ...The use of antibiotics for prophylaxis and growth enhancement in livestock farming is on the increase globally. This practice has led to the emergence and spread of antimicrobial-resistant bacteria in livestock. Only limited research has been done to establish the role of cattle farming in antimicrobial resistance. The current study sought to establish the carriage of multi-drug resistance and extended-spectrum beta-lactamase genes in Escherichia coli from farmers, their cattle, and cattle slurry within Kiambu County. A total of 286 (81%) E. coli isolates were recovered from 352 samples analysed. Antibiotic resistance profiles showed 114 (40%) isolates were resistant to ≥3 antimicrobial classes and were considered multidrug-resistant. Among multidrug-resistant (MDR) E. coli strains, 40 (14%) were resistant to 3 different antimicrobial classes, while 71 (25%) were resistant to between 4 and 7 antibiotic classes. Extended-spectrum β-lactamase resistance was found in 18 isolates: human (n = 14), cattle (n = 2), and environmental (n = 2). Both the bla<sub>CTX-M</sub> and bla<sub>TEM</sub> genes were detected in 10 and 15 strains, respectively. Sequence analysis showed that the isolates carried the bla<sub>TEM-116</sub> (n = 7), bla<sub>TEM-1</sub> (n = 5), and bla<sub>CTX-M-15</sub> (n = 8) genes. Genotyping MDR isolates using (GTG) <sub>5</sub> PCR demonstrated that the isolates were not clonal. This data shows antimicrobial resistance profiles and different types of resistance genes in the E. coli population on dairy farms. As a result, more effective, targeted public health policies and measures need to be put in place to control and prevent the emergence and spread of resistant bacteria.展开更多
[Objective] The aim of this study was to explore the sterilization effects on Escherichia coli by adding bacterial inhibitor(CaCN2)during the process of cattle manure composting so as to provide a theoretical basis fo...[Objective] The aim of this study was to explore the sterilization effects on Escherichia coli by adding bacterial inhibitor(CaCN2)during the process of cattle manure composting so as to provide a theoretical basis for cattle manure harmless treatment.[Method] Both experimental groups supplemented with 2.0% bacterial inhibitor and control groups without bacterial inhibitor were cultured under different temperatures(20,30,37,50,60 ℃)to determine the optimal composing temperature.Under 30 ℃,different bacterial inhibitor doses(0,2.0%,2.5%,3.0%)were added into the compost to obtain the optimal bacterial inhibitor addition dose.[Result] 30,50 and 60 ℃ were ideal temperatures for sterilization of E.coli.Under 30 ℃,E.coli couldn't be detected in 2.5% dose group and 3.0% dose group after culture for 48 h,demonstrating no less than 2.5% bacterial inhibitor should be added.[Conclusion] It has an important significance to enhance the sterilization effects on E.coli by adding CaCN2 into cattle manure compost especially in winter.展开更多
Three experiments were conducted to evaluate direct-fed microbial (<strong>DFM</strong>) supplementation on live performance, carcass characteristics, and fecal shedding of <em>E. coli</em> in ...Three experiments were conducted to evaluate direct-fed microbial (<strong>DFM</strong>) supplementation on live performance, carcass characteristics, and fecal shedding of <em>E. coli</em> in feedlot steers. In Exp. 1, 400 steers (BW = 348 kg) were assigned to treatments: <strong>CON</strong> = lactose carrier only, <strong>BOV</strong> =<em> P. freudenreichii </em>(NP24) +<em> L. acidophilus</em> (NP51), <strong>BOVD</strong> = <em>P. freudenreichii</em> (NP24) +<em> L. acidophilus</em> (NP51), and <strong>COMB</strong> = BOV fed for the first 101 d on feed, followed by BOVD for the final 28 d prior to harvest. In Exp. 2 (n = 1800;BW = 354 kg) and Exp. 3 (n = 112;BW = 397 kg), steers were utilized in a randomized complete block design and assigned to DFM treatments using low dose and high dose, respectively. Fecal samples were collected prior to harvest and analyzed for <em>E. coli</em> serogroups. In Exp. 1, DFM reduced (P < 0.01) the concentration of<em> E. coli</em> O157. Prevalence of O157 was reduced by BOVD supplementation in Exp. 2 and 3 (P < 0.01 and P = 0.08, respectively), and concentration of <em>E. coli</em> O157 in positive samples was reduced in both experiments where enumeration was performed (P ≤ 0.02). Weighted mean differences across the three experiments were equal to a 33% reduction in the prevalence of E. coli O157:H7 in BOVD treated cattle. A significant reduction in prevalence of O26, O45, O103, and O121 was observed in Exp. 2 (P ≤ 0.03). These results indicate that high levels of <em>L. acidophilus</em> (NP51) may represent an effective pre-harvest food safety intervention to reduce fecal shedding of several <em>E. coli</em> serogroups.展开更多
基金Support by Budget Implementation Registration Form of Bogor Agricultural University(No.571/IT3.11/PL/2015)
文摘Objective: To determine the occurrence of CTX-M producing Escherichia coli(E. coli)from cattle feces in Bogor slaughterhouse, Indonesia.Methods: A total of 220 cattle feces samples were collected from Bogor slaughterhouse from March to April 2015. Presence of extended-spectrum beta-lactamase(ESBL) producing E. coli was detected by disc diffusion test based on the recommendation from Clinical and Laboratory Standards Institute(2014). Bacterial strains which were confirmed as producing ESBLs were further analyzed for the presence of bla genes of the ESBL by PCR.Results: The results showed that CTX-M producing E. coli isolates were detected in 19 samples from 220 samples(8.6%). The b-lactamase genes detected were CTX-M-1(n = 10) and CTX-M-9(n = 9). All of the CTX-M producing E. coli isolates showed multidrug resistance phenotypes to at least four antibiotics. The highest incidence of antibiotics resistance was showed to ampicillin(100.0%), cefotaxime(100.0%), and cefpodoxime(100.0%), followed by streptomycin(84.3%), trimethoprim-sulfamethoxazole(73.7%), erythromycin(52.6%), kanamycin(26.3%), doxycycline(10.5%), and ceftazidime(0.0%).Conclusions: Detection of CTX-M-producing E. coli in cattle feces raises important questions as they can represent a potential risk factor to public health.
基金Supported by the Fundamental Research Funds for the Central Universities:the Young Teachers Fund of Southwest Minzu University(2017NZYQN38)Innovative Scientific Research Project for Postgraduates of Southwest Minzu University(CX2018SZ42)
文摘This study was conducted to investigate the drug sensitivity of Escherichia coli isolated from sheep, providing data reference for clinical medication. Fifty-four samples were collected from a certain sheep farm in Yangling, Shaanxi Province for E. coli isolation and identification, and K-B disk diffusion method was used to carry out the dug sensitivity test of 40 E. coli to the eight antimicrobials of spectinomycin, amikacin, tobramycin, enrofloxacin, nalidixic acid, levofloxacin, salafloxacin and ciprofloxacin. The results showed that there were 40 positive E. coli strains and the isolation rate was 74.7%. The resistance rates of the 40 E. coli to spectinomycin, amikacin,tobramycin and nalidixic acid were 85%, 95%, 97.5% and 87.5%, respectively, while the resistance rate to enrofloxacin, levofloxacin, salafloxacin and ciprofloxacin were relatively low and the resistance rates were 35%, 20%, 25% and 17.5%, respectively. The isolates showed different levels of drug resistance to eight antimicrobials and the highest resistance rate was 97.5%. It reminds that the sheep farm should strictly control the dosage according to the antimicrobial sensitive data in clinic.
文摘[ Objective] In order to provide a reference for prevention and control of contagious pleuropneumonia and E. coli mixed infection. [ Meth- od] A comprehensive introduction of the two diseases was given on the following aspects： morbidity, clinical symptoms, pathological changes, la- boratory diagnosis, treatment, prevention, and prevention and control measures. [ Results] Sound development of sheep production was seriously influenced by the two diseases, effective prevention and control measures must be adopted to inhibit the occurrence and prevalence of sheep conta- gious pleuropneumonia and colibacillosis. [ Conclusion] The two epidemic diseases were controllable.
文摘The use of antibiotics for prophylaxis and growth enhancement in livestock farming is on the increase globally. This practice has led to the emergence and spread of antimicrobial-resistant bacteria in livestock. Only limited research has been done to establish the role of cattle farming in antimicrobial resistance. The current study sought to establish the carriage of multi-drug resistance and extended-spectrum beta-lactamase genes in Escherichia coli from farmers, their cattle, and cattle slurry within Kiambu County. A total of 286 (81%) E. coli isolates were recovered from 352 samples analysed. Antibiotic resistance profiles showed 114 (40%) isolates were resistant to ≥3 antimicrobial classes and were considered multidrug-resistant. Among multidrug-resistant (MDR) E. coli strains, 40 (14%) were resistant to 3 different antimicrobial classes, while 71 (25%) were resistant to between 4 and 7 antibiotic classes. Extended-spectrum β-lactamase resistance was found in 18 isolates: human (n = 14), cattle (n = 2), and environmental (n = 2). Both the bla<sub>CTX-M</sub> and bla<sub>TEM</sub> genes were detected in 10 and 15 strains, respectively. Sequence analysis showed that the isolates carried the bla<sub>TEM-116</sub> (n = 7), bla<sub>TEM-1</sub> (n = 5), and bla<sub>CTX-M-15</sub> (n = 8) genes. Genotyping MDR isolates using (GTG) <sub>5</sub> PCR demonstrated that the isolates were not clonal. This data shows antimicrobial resistance profiles and different types of resistance genes in the E. coli population on dairy farms. As a result, more effective, targeted public health policies and measures need to be put in place to control and prevent the emergence and spread of resistant bacteria.
基金Supported by Key Project of Natural Science Foundation of Inner Mongolia Autonomous Region(200607010403)National Key Project of Scientific and Technical Supporting Programs Funded by Ministry of Science&Technology of China(2006BAD04A15)~~
文摘[Objective] The aim of this study was to explore the sterilization effects on Escherichia coli by adding bacterial inhibitor(CaCN2)during the process of cattle manure composting so as to provide a theoretical basis for cattle manure harmless treatment.[Method] Both experimental groups supplemented with 2.0% bacterial inhibitor and control groups without bacterial inhibitor were cultured under different temperatures(20,30,37,50,60 ℃)to determine the optimal composing temperature.Under 30 ℃,different bacterial inhibitor doses(0,2.0%,2.5%,3.0%)were added into the compost to obtain the optimal bacterial inhibitor addition dose.[Result] 30,50 and 60 ℃ were ideal temperatures for sterilization of E.coli.Under 30 ℃,E.coli couldn't be detected in 2.5% dose group and 3.0% dose group after culture for 48 h,demonstrating no less than 2.5% bacterial inhibitor should be added.[Conclusion] It has an important significance to enhance the sterilization effects on E.coli by adding CaCN2 into cattle manure compost especially in winter.
文摘Three experiments were conducted to evaluate direct-fed microbial (<strong>DFM</strong>) supplementation on live performance, carcass characteristics, and fecal shedding of <em>E. coli</em> in feedlot steers. In Exp. 1, 400 steers (BW = 348 kg) were assigned to treatments: <strong>CON</strong> = lactose carrier only, <strong>BOV</strong> =<em> P. freudenreichii </em>(NP24) +<em> L. acidophilus</em> (NP51), <strong>BOVD</strong> = <em>P. freudenreichii</em> (NP24) +<em> L. acidophilus</em> (NP51), and <strong>COMB</strong> = BOV fed for the first 101 d on feed, followed by BOVD for the final 28 d prior to harvest. In Exp. 2 (n = 1800;BW = 354 kg) and Exp. 3 (n = 112;BW = 397 kg), steers were utilized in a randomized complete block design and assigned to DFM treatments using low dose and high dose, respectively. Fecal samples were collected prior to harvest and analyzed for <em>E. coli</em> serogroups. In Exp. 1, DFM reduced (P < 0.01) the concentration of<em> E. coli</em> O157. Prevalence of O157 was reduced by BOVD supplementation in Exp. 2 and 3 (P < 0.01 and P = 0.08, respectively), and concentration of <em>E. coli</em> O157 in positive samples was reduced in both experiments where enumeration was performed (P ≤ 0.02). Weighted mean differences across the three experiments were equal to a 33% reduction in the prevalence of E. coli O157:H7 in BOVD treated cattle. A significant reduction in prevalence of O26, O45, O103, and O121 was observed in Exp. 2 (P ≤ 0.03). These results indicate that high levels of <em>L. acidophilus</em> (NP51) may represent an effective pre-harvest food safety intervention to reduce fecal shedding of several <em>E. coli</em> serogroups.
