OBJECTIVE To investigate the effect of quercetin on primary cultured newborn rat cortex neuron cell which is estrogen depletion,and discuss the possible mechanism,to provide new ideas and strategies for developing a d...OBJECTIVE To investigate the effect of quercetin on primary cultured newborn rat cortex neuron cell which is estrogen depletion,and discuss the possible mechanism,to provide new ideas and strategies for developing a drug of neurodegenerative disease.METHODS Rat cortex neurons were isolated from one day old Sprague Dawley rats and treated with estrogen,quercetin and estrogen receptor antagonists(ICI182,780).Cell viability was determined by MTT assay,neurite outgrowth was measured by fluorescent microsope and estrogen receptors were determine by Western blot.RESULTS Quercetin functions like estrogen to increase cortex neuronal cell viability,the Que(50,100μmol·L^(-1))group compared with the control group could significantly improve the activity of the cortical neurons(P<0.05).It can also increase neurite out growth,the Que(50,100μmol·L^(-1))group significantly promoted the formation of synapse,most of the neurons were full,and the synapses of neurons became thick,growth,and connect to a dense neural network.And in the Western blot experiments,Que(50,100μmol·L^(-1))group could obviously increase the expression of estrogen receptor alpha protein,in addition,the neural protective effect of quercetin can be inhibited by ICI182,780.CONCLUSION Quercetin like estrogen can protected cortex neuronal and the effect of quercetin on cortex neuronal cells was mediated by estrogen receptor alpha.展开更多
目的:针对蜂王浆(RJ)的营养成分、功效,以及在围绝经期女性中的应用和疗效进行综述,以期为RJ的进一步研究和应用提供参考。方法:通过查阅Web of Science、CNKI、PubMed、EMBASE等数据库对RJ研究的国内外文献进行搜集、整理、选择、提炼...目的:针对蜂王浆(RJ)的营养成分、功效,以及在围绝经期女性中的应用和疗效进行综述,以期为RJ的进一步研究和应用提供参考。方法:通过查阅Web of Science、CNKI、PubMed、EMBASE等数据库对RJ研究的国内外文献进行搜集、整理、选择、提炼,并做出综合性介绍和阐述。结果:RJ是一种具有多种功效的蜂产品,在围绝经期女性中,它可通过激活雌激素受体起到改善围绝经期综合征症状评分、泌尿生殖系统症状以及血脂水平的作用。RJ安全性较高,过敏反应罕见,激素含量通常远低于牛奶或一般动物源性食物的雌激素检测限或定量限。结论:RJ可缓解围绝经期综合征症状、改善围绝经期女性的血脂水平。展开更多
Semiquantitive PCR is a method to evaluate expression of target genes by analyzing RT-PCR products in comparison to standards.Two measures are involved;one is that primers for target gene and internal standard are put...Semiquantitive PCR is a method to evaluate expression of target genes by analyzing RT-PCR products in comparison to standards.Two measures are involved;one is that primers for target gene and internal standard are put into the same tube to run PCR,and the other one is that primers for target gene and internal standard run PCR in separate tubes.In research of environmental endocrine disruption semiquantitive PCR is a usual means to measure estrogen-like effects which result from endocrine disruptors.In order to discuss advantages and disadvantages of these two methods for further research on endocrine disruption in Carassius auratus,RT-PCR was used to evaluate of expression of 776 bp of estrogen receptor gene(ER gene) in liver after taking the injection of E2 in C.auratus by following these two methods respectively,with 18S as internal standard.The results showed that ER gene and 18S gene were amplified successfully following both methods.The first method-putting primers for different gene in the same tube to run PCR might contain problems such as competition and different plat phase between target gene and internal standard,and it is needed to grope appropriate ratio of primers and numbers of cycles,yet it is more accurate.And another method putting primers for different genes in separate tubes to run PCR is easier because of the absence of no problemsexisted in the first method,whereas it is less accurate than the first one.In addition,based on the results,18S is an ideal internal standard for both methods in the research of estrogen-like effects in C.auratus.展开更多
基金supported by Science and Technology Research Project of Hebei Higher School,Hebei Education Department(ZD2015131)Natural Science Foundation of Hebei Province(H2012405016)
文摘OBJECTIVE To investigate the effect of quercetin on primary cultured newborn rat cortex neuron cell which is estrogen depletion,and discuss the possible mechanism,to provide new ideas and strategies for developing a drug of neurodegenerative disease.METHODS Rat cortex neurons were isolated from one day old Sprague Dawley rats and treated with estrogen,quercetin and estrogen receptor antagonists(ICI182,780).Cell viability was determined by MTT assay,neurite outgrowth was measured by fluorescent microsope and estrogen receptors were determine by Western blot.RESULTS Quercetin functions like estrogen to increase cortex neuronal cell viability,the Que(50,100μmol·L^(-1))group compared with the control group could significantly improve the activity of the cortical neurons(P<0.05).It can also increase neurite out growth,the Que(50,100μmol·L^(-1))group significantly promoted the formation of synapse,most of the neurons were full,and the synapses of neurons became thick,growth,and connect to a dense neural network.And in the Western blot experiments,Que(50,100μmol·L^(-1))group could obviously increase the expression of estrogen receptor alpha protein,in addition,the neural protective effect of quercetin can be inhibited by ICI182,780.CONCLUSION Quercetin like estrogen can protected cortex neuronal and the effect of quercetin on cortex neuronal cells was mediated by estrogen receptor alpha.
文摘目的:针对蜂王浆(RJ)的营养成分、功效,以及在围绝经期女性中的应用和疗效进行综述,以期为RJ的进一步研究和应用提供参考。方法:通过查阅Web of Science、CNKI、PubMed、EMBASE等数据库对RJ研究的国内外文献进行搜集、整理、选择、提炼,并做出综合性介绍和阐述。结果:RJ是一种具有多种功效的蜂产品,在围绝经期女性中,它可通过激活雌激素受体起到改善围绝经期综合征症状评分、泌尿生殖系统症状以及血脂水平的作用。RJ安全性较高,过敏反应罕见,激素含量通常远低于牛奶或一般动物源性食物的雌激素检测限或定量限。结论:RJ可缓解围绝经期综合征症状、改善围绝经期女性的血脂水平。
文摘Semiquantitive PCR is a method to evaluate expression of target genes by analyzing RT-PCR products in comparison to standards.Two measures are involved;one is that primers for target gene and internal standard are put into the same tube to run PCR,and the other one is that primers for target gene and internal standard run PCR in separate tubes.In research of environmental endocrine disruption semiquantitive PCR is a usual means to measure estrogen-like effects which result from endocrine disruptors.In order to discuss advantages and disadvantages of these two methods for further research on endocrine disruption in Carassius auratus,RT-PCR was used to evaluate of expression of 776 bp of estrogen receptor gene(ER gene) in liver after taking the injection of E2 in C.auratus by following these two methods respectively,with 18S as internal standard.The results showed that ER gene and 18S gene were amplified successfully following both methods.The first method-putting primers for different gene in the same tube to run PCR might contain problems such as competition and different plat phase between target gene and internal standard,and it is needed to grope appropriate ratio of primers and numbers of cycles,yet it is more accurate.And another method putting primers for different genes in separate tubes to run PCR is easier because of the absence of no problemsexisted in the first method,whereas it is less accurate than the first one.In addition,based on the results,18S is an ideal internal standard for both methods in the research of estrogen-like effects in C.auratus.
