RACK1蛋白是一个高度保守的支架蛋白,介导了植物细胞许多信号转导途径,对许多重要的生物学过程起关键调控作用,包括种子萌发、幼苗生长、根系发育、激素介导的信号转导、对非生物胁迫的应答、植物的免疫防御等。南方科技大学梁建生教授...RACK1蛋白是一个高度保守的支架蛋白,介导了植物细胞许多信号转导途径,对许多重要的生物学过程起关键调控作用,包括种子萌发、幼苗生长、根系发育、激素介导的信号转导、对非生物胁迫的应答、植物的免疫防御等。南方科技大学梁建生教授团队于2023年3月在《Journal of Integrative Plant Biology》上发表了题为“Scaffold protein RACK1A positively regulates leaf senescence by coordinating the EIN3-miR164-ORE1 transcriptional cascade in Arabidopsis”的研究论文。展开更多
The plant signaling pathway that regulates pathogen-associated molecular pattern(PAMP)-triggered immunity(PTI)involves mitogen-activated protein kinase(MAPK)cascades that comprise sequential activation of several prot...The plant signaling pathway that regulates pathogen-associated molecular pattern(PAMP)-triggered immunity(PTI)involves mitogen-activated protein kinase(MAPK)cascades that comprise sequential activation of several protein kinases and the ensuing phosphorylation of MAPKs,which activate transcription factors(TFs)to promote downstream defense responses.To identify plant TFs that regulate MAPKs,we investigated TF-defective mutants of Arabidopsis thaliana and identified MYB44 as an essential constituent of the PTI pathway.MYB44 confers resistance against the bacterial pathogen Pseudomonas syringae by cooperating with MPK3 and MPK6.Under PAMP treatment,MYB44 binds to the promoters of MPK3 and MPK6 to activate their expression,leading to phosphorylation of MPK3 and MPK6 proteins.In turn,phosphorylated MPK3 and MPK6 phosphorylate MYB44 in a functionally redundant manner,thus enabling MYB44 to activate MPK3 and MPK6 expression and further activate downstream defense responses.Activation of defense responses has also been attributed to activation of EIN2 transcription by MYB44,which has previously been shown to affect PAMP recognition and PTI development.AtMYB44 thus functions as an integral component of the PTI pathway by connecting transcriptional and posttranscriptional regulation of the MPK3/6 cascade.展开更多
木薯是一种重要的能源和淀粉作物,具有较强的抗逆性和耐贫瘠等能力。为研究木薯中EIN3基因如何被逆境信号调控,对木薯基因组数据库中的EIN3基因进行了序列分析,表明其具有典型的EIN3-like protein DNA结合结构域;其启动子区具有茉莉酸...木薯是一种重要的能源和淀粉作物,具有较强的抗逆性和耐贫瘠等能力。为研究木薯中EIN3基因如何被逆境信号调控,对木薯基因组数据库中的EIN3基因进行了序列分析,表明其具有典型的EIN3-like protein DNA结合结构域;其启动子区具有茉莉酸响应元件等多种响应元件。本研究以木薯品种"华南八号"悬浮培养细胞为材料,利用q RT-PCR检测木薯EIN3基因在乙烯利和茉莉酸甲酯处理后的表达特性。结果显示:EIN3在乙烯处理后,基因表达在8 h内呈现持续上升趋势;而茉莉酸甲酯处理后则整体呈现先上升后下降的趋势,且在2 h基因表达量最大。说明EIN3基因可被乙烯和茉莉酸信号调控,并推测其可受不同激素信号的整合后的综合调控,可参与到植物体内一系列多种生理生化过程。展开更多
文摘RACK1蛋白是一个高度保守的支架蛋白,介导了植物细胞许多信号转导途径,对许多重要的生物学过程起关键调控作用,包括种子萌发、幼苗生长、根系发育、激素介导的信号转导、对非生物胁迫的应答、植物的免疫防御等。南方科技大学梁建生教授团队于2023年3月在《Journal of Integrative Plant Biology》上发表了题为“Scaffold protein RACK1A positively regulates leaf senescence by coordinating the EIN3-miR164-ORE1 transcriptional cascade in Arabidopsis”的研究论文。
基金supported by the Natural Science Foundation of China(grant numbers 31772247,32072399,32170202)the Natural Science Foundation of Shandong Province(grant numbers ZR2020MC113,ZR2020MC120,ZR2020QC126).
文摘The plant signaling pathway that regulates pathogen-associated molecular pattern(PAMP)-triggered immunity(PTI)involves mitogen-activated protein kinase(MAPK)cascades that comprise sequential activation of several protein kinases and the ensuing phosphorylation of MAPKs,which activate transcription factors(TFs)to promote downstream defense responses.To identify plant TFs that regulate MAPKs,we investigated TF-defective mutants of Arabidopsis thaliana and identified MYB44 as an essential constituent of the PTI pathway.MYB44 confers resistance against the bacterial pathogen Pseudomonas syringae by cooperating with MPK3 and MPK6.Under PAMP treatment,MYB44 binds to the promoters of MPK3 and MPK6 to activate their expression,leading to phosphorylation of MPK3 and MPK6 proteins.In turn,phosphorylated MPK3 and MPK6 phosphorylate MYB44 in a functionally redundant manner,thus enabling MYB44 to activate MPK3 and MPK6 expression and further activate downstream defense responses.Activation of defense responses has also been attributed to activation of EIN2 transcription by MYB44,which has previously been shown to affect PAMP recognition and PTI development.AtMYB44 thus functions as an integral component of the PTI pathway by connecting transcriptional and posttranscriptional regulation of the MPK3/6 cascade.
