The endogenous hydrogen sulfide （H2S）, as a new gasotransmitter, participates in many plant physio- logical processes. In this study, we found that both ethylene and drought strongly induced HaS synthetase AtD-CDes ...The endogenous hydrogen sulfide （H2S）, as a new gasotransmitter, participates in many plant physio- logical processes. In this study, we found that both ethylene and drought strongly induced HaS synthetase AtD-CDes gene expression and enzymatic activity. H2S synthesis inhibitors restrained the ethylene and drought induced stomatal closure in Arabidopsis. The H2S synthetase mutant Atd-cdes was insensitive to ethylene and drought, and overexpression of AtD-CDes conferred the transgenic plants more sensitive to ethylene and drought. Sequence analysis of AtD-CDes promoter showed that it contained ethylene response cis element ERE, and abiotic stress responsive cis elements MBS, LTR, and ABRE. The AtD- CDes promoter fused with GUS was transformed into Arabidopsis thaliana to get AtD-CDes promoter：：GUS transgenic plants. When treated with ethylene and drought stress, the enzymatic activity of fl-glucuronidase was higher in the leaves and stomata of transgenic Arabidopsis. Analyses of GUS activity from the transgenic plants har- boring different fragments of the promoter shows that the key section of AtD-CDes promoter response to ethylene was from the -697 to -408 bp, and the key section response to drought stress was from -90 to -1 bp. These results suggested that the H2S produced from AtD-CDesmay mediate ethylene and drought-induced stomatal movement.展开更多
基金supported by the National Natural Science Foundation of China (31170237, 31572107, 31401844, 31540090)
文摘The endogenous hydrogen sulfide （H2S）, as a new gasotransmitter, participates in many plant physio- logical processes. In this study, we found that both ethylene and drought strongly induced HaS synthetase AtD-CDes gene expression and enzymatic activity. H2S synthesis inhibitors restrained the ethylene and drought induced stomatal closure in Arabidopsis. The H2S synthetase mutant Atd-cdes was insensitive to ethylene and drought, and overexpression of AtD-CDes conferred the transgenic plants more sensitive to ethylene and drought. Sequence analysis of AtD-CDes promoter showed that it contained ethylene response cis element ERE, and abiotic stress responsive cis elements MBS, LTR, and ABRE. The AtD- CDes promoter fused with GUS was transformed into Arabidopsis thaliana to get AtD-CDes promoter：：GUS transgenic plants. When treated with ethylene and drought stress, the enzymatic activity of fl-glucuronidase was higher in the leaves and stomata of transgenic Arabidopsis. Analyses of GUS activity from the transgenic plants har- boring different fragments of the promoter shows that the key section of AtD-CDes promoter response to ethylene was from the -697 to -408 bp, and the key section response to drought stress was from -90 to -1 bp. These results suggested that the H2S produced from AtD-CDesmay mediate ethylene and drought-induced stomatal movement.
