目的:通过meta分析研究中国前列腺癌患者中TMPRSS2::ETS融合基因的发生率。方法:通过PubMed、Web of Science、Google Scholar、中国知识资源总库(CNKI)、万方医学在线数据库和维普数据库进行系统检索。用QUADAS-2评估偏倚风险,并用Stat...目的:通过meta分析研究中国前列腺癌患者中TMPRSS2::ETS融合基因的发生率。方法:通过PubMed、Web of Science、Google Scholar、中国知识资源总库(CNKI)、万方医学在线数据库和维普数据库进行系统检索。用QUADAS-2评估偏倚风险,并用Stata 12.0和Review Manager 5.3进行meta分析,评估TMPRSS2::ETS融合基因在中国前列腺癌患者中的发生率。结果:共纳入16项研究,发现在中国前列腺癌患者中TMPRSS2::ERG融合基因是TMPRSS2::ETS融合基因家族最主要的类型,其发生率为16.7%,95%可信区间为(14.5%,18.8%)。就样本类型而言,穿刺活检样本的融合基因发生率为17.9%,95%可信区间为(15.8%,20.1%);当使用免疫组化检测技术时,融合基因发生率为16.6%,95%可信区间为(13.0%,20.2%)。结论:中国前列腺癌患者中,TMPRSS2::ERG融合基因为主要类型,但其发生率较低,因此不宜将TMPRSS2::ERG融合基因作为国内前列腺癌患者的诊断标志。展开更多
AIM: To study the expression pattern of ETS2 (erythroblastosisvirus oncogene homolog 2) in human esophageal squamouscell carcinoma (ESCC).METHODS: Reverse transcription polymerase chain reaction(RT-PCR) and Northern b...AIM: To study the expression pattern of ETS2 (erythroblastosisvirus oncogene homolog 2) in human esophageal squamouscell carcinoma (ESCC).METHODS: Reverse transcription polymerase chain reaction(RT-PCR) and Northern blot were performed to examinethe expression level of ETS2 mRNA in 37 pairs of ESCCtissue samples. Western blot and immunohistochemistrywere carried out to check the expression level of ETS2 proteinin 30 pairs of ESCC tissue specimens.RESULTS: RT-PCR and Northern blot analysis showed thatETS2 mRNA upregulated in 75.7 % (28/37) examined ESCCtissues relative to matched normal tissues. From those 37cases, 14 cases were randomly selected to perform Westernblot and the results revealed that ETS2 protein overexpressedin 71.4 % (10/14) checked ESCC tissues compared with thecorresponding normal tissues. Moreover, the expressionpatterns of ETS2 protein in those 14 cases were identical tothose of ETS2 mRNA displayed by RT-PCR or Northern Blot.Immunohistochemistry analysis showed that the expressionlevel of ETS2 protein rose in 75 % (12/16) tumor epithelialcells contrasted to the normal cells. Altogether the expressionlevel of ETS2 protein increased in 73.3 % (22/30) checkedESCC tissue samples contrary to their normal counterparts.CONCLUSION: The results suggested that ETS2overexpressed in paired human ESCC tissue samples at bothmRNA and protein levels and may be associated with thetumorigenesis of esophagus.展开更多
基金China key program on basic research,No. G1998051021The Chinese Hi-tech R&D program(2001AA231041)National Natural Science Foundation of China,No.39993402
文摘AIM: To study the expression pattern of ETS2 (erythroblastosisvirus oncogene homolog 2) in human esophageal squamouscell carcinoma (ESCC).METHODS: Reverse transcription polymerase chain reaction(RT-PCR) and Northern blot were performed to examinethe expression level of ETS2 mRNA in 37 pairs of ESCCtissue samples. Western blot and immunohistochemistrywere carried out to check the expression level of ETS2 proteinin 30 pairs of ESCC tissue specimens.RESULTS: RT-PCR and Northern blot analysis showed thatETS2 mRNA upregulated in 75.7 % (28/37) examined ESCCtissues relative to matched normal tissues. From those 37cases, 14 cases were randomly selected to perform Westernblot and the results revealed that ETS2 protein overexpressedin 71.4 % (10/14) checked ESCC tissues compared with thecorresponding normal tissues. Moreover, the expressionpatterns of ETS2 protein in those 14 cases were identical tothose of ETS2 mRNA displayed by RT-PCR or Northern Blot.Immunohistochemistry analysis showed that the expressionlevel of ETS2 protein rose in 75 % (12/16) tumor epithelialcells contrasted to the normal cells. Altogether the expressionlevel of ETS2 protein increased in 73.3 % (22/30) checkedESCC tissue samples contrary to their normal counterparts.CONCLUSION: The results suggested that ETS2overexpressed in paired human ESCC tissue samples at bothmRNA and protein levels and may be associated with thetumorigenesis of esophagus.
