Comparative microsporogenesis and flower development in Eucalyptus urophylla×E.grandis

Microsporogenesis and flower development in Eucalyptus urophylla × E. grandis were examined using chromosome tableting to provide a method to predict the meiotic stages in this species. Although microsporogenesis was normal, cytokinesis during meiosis of pollen mother cells occurred simultaneously, with strong asynchronism observed in the two different lengths of stamens in a flower bud. In a single flower, the developmental period of microsporogenesis in anthers on the longer stamens was always ahead of those on the shorter stamens. Flower development was also asynchronous at different locations on a branch. Flower buds on the upper side of the branch were larger in diameter than those on the lower side. In addition, a correlation was observed between microsporogenesis development and flower bud diameter growth. The pachy- tene stage was first observed when the diameter of the flower buds increased to 3.0 mm, and the majority of the meiotic stages were observed when bud diameters ranged from 3.5 to 5.0 mm. This study showed that the developmental stages of microsporogenesis in Eucalyptus urophylla × E. grandis could be distinguished readily, which may be applicable to future breeding studies.

Soil microorganisms alleviate the allelopathic effect of Eucalyptus grandis × E. urophylla leachates on Brassica chinensis

Soil microbes may be critical players in determining the allelopathic potential of some plants. Low levels of plant community biodiversity in Eucalyptus plantations have been attributed to the allelopathic potential of these tree species. The role of soil microbes in the allelopathic effect of leaf leachates of the hybrid tree Eucalyptus grandis x E. urophylla, was tested in Petri dish assays with Brassica chinensis as a receiver plant. Soils were collected from either a local garden (soil A) or a Eucalyptus plantation (soil B) and half of each soil was sterilized to remove microbes. These soils were then treated with E. grandis x E. urophylla leachates for 0-72 h. Seed germination of B. chinensis was significantly inhibited in soils treated with leaf leachates relative to untreated soils. The inhibitory effect of the leaf leachates was more pronounced in sterilized soils. Total phenolic content was obvious lower in nonsterile leachate-treated soils than in sterile soils. Biomass of B. chinensis was negatively correlated with the total phenolic content in soils. Our findings suggest that soil microbes can alleviate the allelopathic potential of Eucalyptus and thereby its negative impact on plant growth.

Acacia mangium and Eucalyptus urophylla in Northern Vietnam： The Relationship between Silvicultural Activity and Quality of Stand

This paper sets out to determine the link between socio-economic attributes of smallholders, silvicultural activity and the stand quality of Acacia mangium and Eucalyptus urophylla plantations in two unrelated communes in Phu Tho province, Vietnam. The necessary data was collected by interviewing the smallholders and conducting inventory of the stands. The data was analyzed with descriptive statistics, Mann-Whitney tests and Spearman correlations. More silvicultural practices were conducted by A. mangium planters with larger areas. Most of the smallholders practiced the suggested silvicultural activities. Productivity was affected by pruning in medium quality sites. This research recommends that smallholders were supported with incentives for the plantation establishment, education for smallholders in silvicultural techniques, organization and development activities for the wider market.

广林九号桉叶精油的抑菌活性研究

研究了广林九号桉[Eucalyptus urophylla×Eucalyptus grandis(Guanglin 9)]叶精油的抑菌活性。利用气相色谱-质谱联用技术分析桉叶精油的化学成分,采用滤纸片法评价其抑菌活性,以平板稀释法测定了精油的最小抑菌浓度。广林九号桉叶精油的主要成分有:1,8-桉叶油素(40.844%)、α-蒎烯(24.221%)、α-松油醇(4.661%)、反式-松香芹醇(3.735%)、龙脑(2.762%)、乙酸α-松油脂(3.592%)和松香芹酮(1.978%)。广林九号桉叶精油对测试的菌种均有良好的抑菌活性,但对细菌和霉菌的抑菌效果有所差异。对金黄色葡萄球菌(Staphylococus aureus)、枯草芽孢杆菌(Bacillus subtilis)、大肠杆菌(Escherichia coli),黄曲霉(Aspergillus flavus)、黑曲霉(Aspergillus niger)和橘青霉(Penicillum citrinum)的最小抑菌浓度分别为10、20、20、10、10、5 mL/L。

Study on Machining Properties of Eucalyptus urophylla×E. grandis:I. Planing and Sanding Properties

The research method in this paper is based on the standard of American Society for Testing andMaterials (ASTM). Planing and sanding are selected to study the machining properties of E. urophylla × E.grandis plantation wood. Moreover, the reasons for machining defects are analyzed. The results show E.urophylla × E. grandis planted in south China is a good species with a great potential for solid woodutilization.

Growth Process of Eucalyptus urophylla × E.grandis Stand Based on Logistic Equation

Eucalyptus is the most valuable cultivated forest genus in the tropical and subtropical areas nowadays. It has been a challenge for foresters to model growth due to the genetic variations, management regimes, and multiple products generated from the plantations. In this paper, Logistic equation was used to study the stock growth process of E. urophylla × E. grandis plantation at age of 14 with 6 spacing treatments. And the biological interpretation of the parameters of Logistic equation was analyzed. The results show that it is flexible, precise and accurate to fit the growth process.

Diversity of mycorrhizal fungi and soil indicative species in coastal plantations of northeast Brazil

The aim of this work was to evaluate arbuscular mycorrhizal(AM)fungi as soil indicators and the mycorrhization of native and exotic tree species planted in the Acaraúbasin,a transition area from the coast to the Brazilian semiarid region.Plots with 6-year-old trees of four native and three non-native species as well as one non-forested area were evaluated in terms of the diversity of AM fungi in the mycorrhizosphere and the root colonization by AM and ectomycorrhizal(EcM)fungi.Twenty-four AM fungi were identified;Claroideoglomus etunicatum,Glomus sinuosum,Paraglomus albidum,Acaulospora laevis,and Acaulospora brasiliensis were abundant in the forest soil.Diversity,dominance,evenness and richness indices of AM fungi were higher in plots with native trees.All root samples were colonized by AM fungi and only A nadenanthera colubrina,Acacia mangium,Casuarina equisetifolia and Eucalyptus urophylla formed associations with EcM fungi.Acaulospora morphotypes served as soil indicators for coverings with the native species Astronium fraxinifolium and Colubrina glandulosa.Exotic species may favor the proliferation of rarer AM fungi.These fungi–plant relationships may be important in the management of forest systems,and the evidence with mycorrhizal associations allows the inclusion of Brazilian species in tropical reforestation.

Influence of Planting Spacing on Mechanical Properties of Eucalyptus grandis × E. urophylla Planted in China

The objective of this study was to understand influence of planting spacing on mechanical properties of Eucalyptus grandis × E. urophylla planted as a potential species for solid wood products in China. Four trees at age of 13 were selected from each of 6 groups of planting spacing and a total of 24 sampling trees were selected from the Dongmen Forestry Center of Guangxi Province in China. Furthermore, a one-meter-long log from each tree was cut into small blocks for wood properties testing. MOE, MOR and compression strength parallel to wood grain were determined in accordance with Chinese standards. In addition, wood density and shrinkage properties were measured. Statistical analysis by SAS software indicated significant differences among the 6 groups of planting spacings on the air-dried density, radial shrinkage and bending MOR properties at the 0.05 level, and also on the compression strength at the 0.10 level. However, it was observed that there is no significant differences among shrinkages in tangential and volume, and MOE.

Leaf water potential and gas exchange of eucalypt clonal seedlings to leaf solar protectant

This experiment was carried out in acclimatized greenhouses with seedlings of two hybrid clones of Eucalyptus urophylla×Eucalyptus grandis. A sunscreen protector consisting of 62.5% calcium carbonate was sprayed on the seedlings at weekly intervals. Water stress was induced by suspending irrigation until the soil reached 30% available water and water was then replaced so that it returned to field capacity. Gas exchange and leaf water status were measured after 50 days. The experiment was set up in a 4×2 factorial randomized block design in four distinct environments:(1) temperatures less than 21.2℃ and vapor pressure deficit of 0.15 kPa;(2) intermediate temperatures of 24.2℃ and vapor pressure deficit of 0.69 kPa;(3) high temperatures of 27.0℃ and high vapor pressure deficit of 1.4 kPa; and,(4) high temperature of 27.0℃ and vapor pressure deficit below 1.10 kPa. Two leaf sun protector treatments were used, with five replications each. High atmospheric demand acted as a stress factor for the seedlings during the initial growth phase.Applications of leaf sunscreen protector provided beneficial effects in maintaining optimum water status and gas exchanges of the plants under water stress.

Study on Machining Properties of Eucalyptus urophylla× E.grandis:Ⅱ.Shaping,Boring,Mortising and Turning

The research method of this paper is based on the standards of American Society for Testing and Materials (ASTM). Four items that contain boring, mortising, shaping and turning are selected to study the machining properties of E. urophylla × E. grandis plantation wood. The reasons for machining defects are analyzed. The resultshows thatE. urophylla × E. grandis planted in South China isa good specieswith great potential for solid wood utilization.

Study on Growth and Genetic Gains of Different Families in Eucalyptus urophylla Second Generation Seed Orchards

A second-generation seedling seed orchard including 32 open-pollinated families of Eucalyptus urophylla was built at Gaoyao City, Guangdong Province to examine the genetic performance of the orchard, which were selected from the superior trees in an improvement seed orchard. Compared to the characteristics of height, DBH, individual volume, stem form, branch and blossom quantities among different families at 22 months. The results indicate that: (1) The most superior families in individual volume and bl...

Effect of Initial Moisture Content on Solar Drying Ratefor Eucalyptus grandis × E. urophylla

Wood drying is one of the most important process links in wood products processing.However, the existing drying methods mainly depend on heat to desiccate wood, which makes wooddrying consume roughly 40% - 70% of the total energy in the entire wood process. Using solar energyfor drying is greatly significant in two aspects of energy conservation and environment protection. Themoisture content (MC) of lumber affects not only the target value of wood drying, but also the heattransfer properties, moisture rate of expansion, etc., affecting dried lumber quality and drying rate.Eucalyptus grandis × E.urophylla lumbers were collected from Guangdong as test materials and dried inthe solar dryer with low temperature schedule and the effect of initial moisture content were primarystudied on drying rate. Experiments showed that solar drying was suitable for timber with high MC. Interms of drying rate and dried lumber quality, solar drying was as good as traditional steam drying withthe same schedule. And the solar dryer had distinct energy-saving effect.

不同坡向对尾巨桉人工林林下草本植物多样性的影响

为了解不同坡向对尾巨桉(Eucalyptus urophylla×E.grandis)人工林林下草本植物多样性(Margalef指数dMa、Simpson指数D、Shannon-wiener指数H、Pielou指数J)的影响,以云南省临沧市双江县忙安林场8年生人工林为研究对象,设置不同坡向(阳坡、半阳坡、阴坡、半阴坡)的12个样地进行调查。研究结果显示:1)尾巨桉人工林共调查统计到林下草本植物30种,隶属16科28属,草本层植物物种数在阴坡、半阴坡相同且均高于阳坡、半阳坡。2)不同坡向尾巨桉人工林林下草本物种主要以禾本科的芭茅、菊科的紫茎泽兰为优势物种,占据主导地位。3)不同坡向尾巨桉人工林林下草本层植物在半阴坡的dMa、D、H、J值最大,阳坡最小。综合不同坡向尾巨桉人工林林下植物多样性指数分析比较,阴坡更有利于林下草本植物的生存。

尾巨桉等桉树无性系生长特性分析及选择

对尾巨桉(Eucalyptus urophylla×E.grandis)、巨尾桉(E.grandis×E.urophylla)、尾叶桉(E.urophylla)无性系试验林生长性状和干形进行了分析,为选择优良无性系提供科学依据。结果表明,不同无性系及同一无性系在不同林龄时的胸径、树高、单株材积生长率均有所不同,总体上QL4、QL7无性系生长率较大,而QL1、QL2无性系的生长率则较小;林龄1.5年及6.5年时无性系间胸径、单株材积均有显著差异,而无性系间树高仅在林龄1.5龄时差异显著;其余林龄无性系间的差异均不显著。用6.5年生参试无性系胸径、树高、单株材积、干形观测值对各无性系进行综合评定,结果QL7无性系的综合评定结果最佳,被评为优良无性系,其6.5年生时平均胸径、树高、单株材积分别是16.83 cm、21.42 m、0.233 1 m^3;而QL1无性系综合评定排最后一位;因此,不同无性系及同一无性系在不同阶段的生长表现均有所不同,无性系间胸径、单株材积在其生长的早期及后期均存在显著差异,而树高则在早期差异显著,所选择的QL7优良无性系可在试验地所在区域推广种植。

滇南尾巨桉人工林对不同间伐抚育措施的生长响应及其效益分析

本实验旨在揭示不同抚育措施对尾巨桉(Eucalyptus urophylla×E.grandis)人工林生长的影响,为滇南地区尾巨桉人工林的培育提供理论依据。以云南省宁洱市小黑江林区4年生尾巨桉林为研究对象,依立地特征将其划分为Ⅰ、Ⅱ、Ⅲ等3个类型,连续4年对样地进行观测,分别进行每木检尺、树高测量以及林下灌木和草本的统计,通过显著性差异分析、连年增长量分析以及生物多样性指数的比较,评价不同间伐措施的抚育效果。结果表明:在Ⅰ类样地中,间伐处理样地伐后第1年树高和胸径的连年增长量均高于对照YP(树高连年增长量约0.5 m/a,胸径连年增长量约1.28 cm/a),间伐强度为30%样地(BTQ1-30%-1/1、CCD1-30%-1/1和MMH1-30%-1/1等)伐后第1年树高和胸径的连年增长量均高于间伐强度为50%的样地(CCD2-50%-1/1和XSHK-50%-1/1等);同时,经过抚育间伐样地的林下植被多样性指数均高于对照样地,间伐强度为50%样地(MZP2-50%-1/1和BTQ2-50%-3/3)的林下灌木种类在各样地中最多(均达到10种),间伐方式为砍三留三样地比砍一留一样地的植被多样性指数高。在Ⅱ类样地中,间伐强度为50%的情况下,砍三留三样地的树高、胸径连年增长量均高于对照样地和砍一留一样地。在Ⅲ类样地中,仅开展间伐抚育样地的林分伐后第1年胸径连年增长量(0.90 cm/a)同对照样地(0.85 cm/a)并没有较大差别,而间伐抚育+施肥措施结合样地的伐后第1年胸径连年增长量(1.35 cm/a)却明显高于对照。在各立地条件样地中,间伐抚育林地的667 m^2蓄积量和经济总产值均大于对照样地,不同间伐措施样地之间也存在一定的差异;其中,Ⅰ类立地中间伐强度为30%,砍一留一样地的667 m^2产量最高,经济产值也最大;在Ⅱ类立地中,间伐强度为50%,砍三留三样地的产量最高,经济产值也明显高于对照和其他样地。可得出结论,间伐抚育能够提高滇南地区尾巨桉人工林胸径的增长量、667 m^2蓄积量以及经济产值,丰富林下植被多样性。其中,间伐强度为30%更适于Ⅰ类立地,间伐方式为砍三留三更适于Ⅱ类立地(间伐强度为50%的情况下),Ⅲ类立地在间伐的同时应该结合施肥等措施才能有效改善林分的生长。同时,砍三留三的间伐方式比砍一留一的间伐方式更有利于丰富林分的林下植被多样性。此外,样地的立地条件越好,进行间伐抚育所取得的效益一般越高。

巨尾桉人工林林木二元形高模型的研建

为研究巨尾桉(Eucalyptus grandis×E.urophylla)人工林林木二元形高模型,利用编制双江县巨尾桉二元立木材积表的138株造材样木实测数据(随机划分编表样本103株,检验样本35株),使用SPSS 21.0软件非线性回归分析工具对8个常用的二元形高备选模型进行拟合,根据分析计算的相关指数(R2)、均方差(RMSE)、平均绝对偏差(MAD)、总相对误差(RS)、模型预估精度(P)等指标对模型进行统计分析和综合评价,结果表明:模型FH=aHb Dc和FH=aHb/Dc的各项评价指标综合得分并列最高,基于国内外林学界在二元形高模型方面的应用传统,本研究选定FH=aHb/Dc作为巨尾桉人工林林木二元形高的编表模型,代入参数值的模型表达式为FH=0.633358 H^(1.356645)/D^(0.493597)。使用35株检验样本实测数据进行适用性检验的结果表明:模型的相关指数(R2)为0.90480,估计值的均方差(RMSE)为0.61151,平均绝对偏差(MAD)为0.50557,总相对误差(RS)为1.561%,预估精度(P)为98.52%;经残差检验,检验样本残差图以横轴为中轴线分布均匀;经进行F检验,计算得F值为0.96,小于F_(0.05)(1,33)的查表值4.14。生产性检验的结果表明:模型的相关指数(R2)为0.99035,估计值的均方差(RMSE)为3.37780,平均绝对偏差(MAD)为2.85151,总相对误差(RS)为1.836%,预估精度(P)为99.48%。编表样本和检验样本的各项评价指标结果表明,模型FH=0.633358 H^(1.356645)/D^(0.493597)的拟合精度高、误差小,适合作为巨尾桉人工林林木的二元形高模型,以此为基础编制的二元形高表可以在生产实践中应用。

Effects of Forest Types and Environmental Factors on Soil Microbial Biomass in a Coastal Sand Dune of Subtropical China

Coastal sand dune ecosystems generally have infertile soil with low water-holding capacity and high salinity. However, many plant species have adapted to the harsh sand environment along the southeastern coast of China. Studying the microbial biomass in such an ecosystem can improve our understanding of the roles that microbes play in soil fertility and nutrient cycling. We investigated the differences in soil microbial biomass carbon(MBC) and nitrogen(MBN) contents and their seasonal dynamics in five forest types(a secondary forest and plantations of Casuarinas, Pine, Acacia, and Eucalyptus). The results indicated that the seasonal variations of soil MBC and MBN contents in all five forest stands were higher in spring and winter, but lower in summer and autumn. The MBC content was lower in the Casuarinas plantation than in the other plantations in the same soil layer. However, no significant differences were observed in MBN contents among the different forest types. The MBC and MBN concentrations were positively correlated with soil moisture, but negatively correlated with soil temperature. The MBC and MBN contents also decreased with increasing soil depth. Across all soil layers, secondary forest had the highest MBC and MBN concentrations. Our study also showed that the MBC and MBN contents were positively affected by total soil carbon(TC), pH, and litter N content, but were negatively impacted by soil bulk density and litter C content. Moreover, the MBN content was positively correlated with root N content. In summary, environmental factors and the differences in litter and fine roots, soil nutrient contents, as well as the soil physical and chemical properties caused by different tree species collectively affected the concentrations of the soil MBC and MBN.

尾叶桉与赤桉正反交杂种F_(1)代生长、材性及其化学组分与抗风性能的分析

以广东江门尾叶桉(Eucalyptus urophylla)与赤桉(Eucalyptus camaldulensis)正反析因交配杂种F_1代9年生测定林为材料,用Excel计算各杂交组合抗风指数、生长性状、材质形状以及木素、综纤素的均值;用R软件对这些性状进行单因素方差分析、相关性分析以及多性状指数综合评价。单因素方差分析表明正交与反交组合杂种的生长性状如树高、胸径、材积和冠幅等差异都不显著;而材质形状如基本密度和纤维宽度差异则较为显著,但纤维长度和纤维长宽比差异不显著;另外,正反交组合杂种的木素和综纤素含量的差异也较为显著。相关性分析表明,抗风指数与木材基本密度及纤维长呈正显著相关性,而与纤维长宽比呈负显著相关性;但抗风指数与树高、胸径、材积、冠幅、纤维宽、木素含量及综纤素含量之间的相关性都不显著;木材基本密度及纤维长与抗风指数的显著相关性为抗风优质的桉树选择提供参考。综合评价结果表明,当各变量权重相同时,正交组合中综合得分前3名的是杂交组合77、81和82;反交组合中综合得分前3名的是杂交组合50、51和29;同时还选出5株优良单株29-3、51-4、77-1、77-3及50-2,为今后大规模人工制种提供优质亲本材料。

引入哑变量的广西尾巨桉人工林地位指数模型拟合效果分析

通过对广西中南部尾巨桉(Eucalyptus urophylla×Eucalyptus grandis)人工林树高—林龄生长规律及其建模的研究,为广西尾巨桉人工林产量预估、间伐抚育培育大径材和林分经营管理提供科学依据。以北回归线以南广西中南部地区118块尾巨桉人工林20 m×20 m的样地为研究对象,利用标准地调查获取的基础数据,对不同立地环境优势木高-树龄生长曲线模型进行拟合,经引入哑变量对最优生长模型进行选择。根据基准年龄时优势木树高变化值,结合生长特性确定指数级间距并划分地位指数级。结果表明:树高生长的最优基础模型和引入哑变量的优化模型均为Richards方程,哑变量模型的R^(2)和RMSE分别为0.983和2.160;单形和多形生长方程的卡方值χ^(2)分别为7.465和5.699,精确度p分别为0.877和0.970;地位指数表编制时,确定基准年龄为6年,并将指数级确定成2 m为间距,划分为6个地位指数级(17、19、21、23、25、27 m)。Richards导向曲线方程和引入哑变量的非线性混合效应模型的精度检验、卡方检验及残差检验均表明:所选理查德生长方程与所编地位指数表适用于研究区域人工林,能客观反映该区域尾巨桉人工林的生长状况。同时,构建的立地质量等级哑变量模型有益于提高建模的精确度和适用性。

尾叶桉全双列杂交试验研究

对江门市新会区会城镇都会2、3.5、12.9年生40个桉树(Eucalyptus urophylla)杂种子代/亲本家系和1个对照CK无性系进行生长性状和形质性状进行方差分析,结果表明:不同林龄的性状生长在家系间、胸径和在单株材积基本都达到显著到极显著差异水平,除少数性状存在异常与林分的保存率有关而未达到显著差异;10.9年生有19个家系的单株材积量超过总体平均值。‘32号’‘40号’50号’和‘55号’4个家系均排名前10且在林分早期和晚期生长中均表现优良,是稳定生长的家系。不同性状之间的相关关系随着林龄的增长具有一致性,表明桉树在生长上随时间具有一定稳定性,为优良家系/单株的早期选择提供参考。综合分析表明,‘21号’作为母本、‘2号’作为父本更易产生优良后代,这两个参试亲本值得杂交育种的长期关注。