The rapid propagation of plantlets of Vaccinium corymbosum in vitro was studied by using the stem-segment with buds as the explant.The optimal culture conditions were set as follows:1)for the initial culture,the expla...The rapid propagation of plantlets of Vaccinium corymbosum in vitro was studied by using the stem-segment with buds as the explant.The optimal culture conditions were set as follows:1)for the initial culture,the explants were sterilized with alcohol,sap nation of cresol and HgCl2,and then cultured on WPM medium containing ZT 3.0 mg·L-1 and 3% sucrose;2)the medium for clump bud induction was WPM + ZT 2.0 mg·L-1 + 3% sugar;3)the medium for robust shoot was WPM + ZT 0～1.0 mg·L-1 + 3% sugar;4)for adventitious root induction from shoots,the medium was 1/2 WPM liquid nutritions containing IBA 1.0～5.0 mg·L-1 and 2% sugar,in which either vermiculite,filter paper or sterilized cotton were added,and shoots were cultured under a photoperiod of 12 h illumination and 12 h darkness alternatively after 14 days in darkness;5)the medium for transplanting was turfy-soil and mosses（V∶V= 1∶1）.展开更多
文摘The rapid propagation of plantlets of Vaccinium corymbosum in vitro was studied by using the stem-segment with buds as the explant.The optimal culture conditions were set as follows:1)for the initial culture,the explants were sterilized with alcohol,sap nation of cresol and HgCl2,and then cultured on WPM medium containing ZT 3.0 mg·L-1 and 3% sucrose;2)the medium for clump bud induction was WPM + ZT 2.0 mg·L-1 + 3% sugar;3)the medium for robust shoot was WPM + ZT 0～1.0 mg·L-1 + 3% sugar;4)for adventitious root induction from shoots,the medium was 1/2 WPM liquid nutritions containing IBA 1.0～5.0 mg·L-1 and 2% sugar,in which either vermiculite,filter paper or sterilized cotton were added,and shoots were cultured under a photoperiod of 12 h illumination and 12 h darkness alternatively after 14 days in darkness;5)the medium for transplanting was turfy-soil and mosses（V∶V= 1∶1）.
