[ Objective] The purpose was to study the occurrence law and control effect of brown patch of E. lathyris. [ Method ] The pathogen of brown patch in E. lathyris was isolated and identified in Xingyi of Guizhou Provinc...[ Objective] The purpose was to study the occurrence law and control effect of brown patch of E. lathyris. [ Method ] The pathogen of brown patch in E. lathyris was isolated and identified in Xingyi of Guizhou Province in 2008 -2009, and disease symptom observation and field fungicide efficacy test were conducted. [ Result ] The pathogen of brown patch in E. lathyris was fungus in Pseudoc ercospora sp., its suitable growth temperature was 20 -35 ℃, its occurrence peak was from June to September, and its field incidence were 30% -60%. Field efficacy test showed that 5 kinds of fungicides such as Thiophanate-methyl, Mancozeb, etc. had certain control effect on the disease, the relative control effect was 54. 3% -82.5%, in which mancozeb had better control effect, while Thiediazole copper had worse control effect. [Conclusion] The study would provide theoretical reference for further research on the cultivation of E. lathyris with hiah vield and aualitv and control of brown hatch.展开更多
Euphorbia factor L2, a lathyrane diterpenoid isolated from caper euphorbia seed(the seeds of Euphorbia lathyris L.), has been traditionally applied to treat cancer. This article focuses on the cytotoxic activity of Eu...Euphorbia factor L2, a lathyrane diterpenoid isolated from caper euphorbia seed(the seeds of Euphorbia lathyris L.), has been traditionally applied to treat cancer. This article focuses on the cytotoxic activity of Euphorbia factor L2 against lung carcinoma A549 cells and the mechanism by which apoptosis is induced. We analyzed the cytotoxicity and related mechanism of Euphorbia factor L2 with an MTT assay, an annexin V-FITC/PI test, a colorimetric assay, and immunoblotting. Euphorbia factor L2 showed potent cytotoxicity to A549 cells. Euphorbia factor L2 led to an increase in reactive oxygen species(ROS) generation,a loss of mitochondrial electrochemical potential, release of cytochrome c, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase, suggesting that Euphorbia factor L2 induced apoptosis through a mitochondrial pathway. The cytotoxic activity of Euphorbia factor L2 in A549 cells and the related mechanisms of apoptotic induction provide support for the further investigation of caper euphorbia seeds.展开更多
基金Supported by Agricultural Scientific and Technological Project in Guizhou Province"Selection and Demonstration of New Variety of Energy Plant Euphorbia lathyris L.with High Oil"(QKH NY[2008]No.3060)~~
文摘[ Objective] The purpose was to study the occurrence law and control effect of brown patch of E. lathyris. [ Method ] The pathogen of brown patch in E. lathyris was isolated and identified in Xingyi of Guizhou Province in 2008 -2009, and disease symptom observation and field fungicide efficacy test were conducted. [ Result ] The pathogen of brown patch in E. lathyris was fungus in Pseudoc ercospora sp., its suitable growth temperature was 20 -35 ℃, its occurrence peak was from June to September, and its field incidence were 30% -60%. Field efficacy test showed that 5 kinds of fungicides such as Thiophanate-methyl, Mancozeb, etc. had certain control effect on the disease, the relative control effect was 54. 3% -82.5%, in which mancozeb had better control effect, while Thiediazole copper had worse control effect. [Conclusion] The study would provide theoretical reference for further research on the cultivation of E. lathyris with hiah vield and aualitv and control of brown hatch.
基金supported by National Natural Science Foundation of China (No. 81473320)Fund from Guangdong Science and Technology Department & Guangdong Academy of Traditional Chinese Medicine (2016A020226024)+1 种基金Fund of Guangdong Education Department (2015KTSCX112)the Science Fund of the Education Bureau of Guangzhou City (1201410039 and 2012C208)
文摘Euphorbia factor L2, a lathyrane diterpenoid isolated from caper euphorbia seed(the seeds of Euphorbia lathyris L.), has been traditionally applied to treat cancer. This article focuses on the cytotoxic activity of Euphorbia factor L2 against lung carcinoma A549 cells and the mechanism by which apoptosis is induced. We analyzed the cytotoxicity and related mechanism of Euphorbia factor L2 with an MTT assay, an annexin V-FITC/PI test, a colorimetric assay, and immunoblotting. Euphorbia factor L2 showed potent cytotoxicity to A549 cells. Euphorbia factor L2 led to an increase in reactive oxygen species(ROS) generation,a loss of mitochondrial electrochemical potential, release of cytochrome c, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase, suggesting that Euphorbia factor L2 induced apoptosis through a mitochondrial pathway. The cytotoxic activity of Euphorbia factor L2 in A549 cells and the related mechanisms of apoptotic induction provide support for the further investigation of caper euphorbia seeds.
