This paper introduced the preparation work before transplanting big olive trees(Olea europaea L.),including key technical measures such as plant excavation,transportation,planting,and post-planting tending.The aim was...This paper introduced the preparation work before transplanting big olive trees(Olea europaea L.),including key technical measures such as plant excavation,transportation,planting,and post-planting tending.The aim was to provide scientific basis and feasible solutions for adjusting the density of olive trees in Shiyan City,and to assist in the high-quality development of the olive industry.展开更多
Olea europaea(Family:Oleaceae)is the characteristic fruit tree of the Mediterranean basin.The oil extracted from the fruit was the essential product,olive oil was used for cooking and as salad oil.It was preferred for...Olea europaea(Family:Oleaceae)is the characteristic fruit tree of the Mediterranean basin.The oil extracted from the fruit was the essential product,olive oil was used for cooking and as salad oil.It was preferred for its flavor and beneficial health effects.While,low grade oil was used in cosmetics,as lubricants and for soap production.Plant parts were traditionally used in the treatment of diabetes,malaria,hypertension,coughs,asthma,lumbago,rheumatism,kidney problems,urinary tract infections,nose bleeding,for eye infections and to relieve sore throats.About 676 distinct chemical compounds were identified in the Olea europaea,they were included(fatty acids,phenolic compounds,alcohols,volatiles,phospholipids,triterpenic acids,sterols,hydrocarbons,sugars,amino acids,tocopherols,pigments,and many other compounds).Olea europaea extracts and oil possessed many pharmacological activities included cardiovascular,anti-obesity,antidiabetic,inflammatory,analgesic,antioxidant,antimicrobial,antiparasitic,anti-anticancer,immunomodulatory,respiratory,endocrine,reproductive and protective effects.This review discussed the contents,pharmacological,nutritional and therapeutic activities of Olea europaea.展开更多
In order to study the possibility of hydroxytyrosol(HT)as a drug,we used SwissADME system to predict ADME of HT and pkCSM system to predict Tox of HT.The results show that hydroxytyrosol meets the Lipinski’s five pri...In order to study the possibility of hydroxytyrosol(HT)as a drug,we used SwissADME system to predict ADME of HT and pkCSM system to predict Tox of HT.The results show that hydroxytyrosol meets the Lipinski’s five principles of drug-like properties.With strong efficacy and pharmacological activity,HT has high drug-likeness degree.With good bioavailability,it can be easily absorbed by the gastrointestinal tract,though not absorbed by skin.Hydroxytyrosol has not only a strong potency and pharmacological activity,but also no liver toxicity and skin allergy.Tox data predicts that it has mutagenic potential,which may be the result of overreduction.展开更多
Objective: To evaluate the impact of Olea (O.) europaea extract on markers of oxidative stress and apoptosis of ovarian tissues in a rat model of torsion/detorsion-induced ovarian damage. Methods: A total of 28 Wistar...Objective: To evaluate the impact of Olea (O.) europaea extract on markers of oxidative stress and apoptosis of ovarian tissues in a rat model of torsion/detorsion-induced ovarian damage. Methods: A total of 28 Wistar female rats were randomly assigned into 4 groups, with 7 rats in each group. The sham group received a 2.5 cm longitudinal incision in the midline part of the abdomen which was then sutured with 5-0 nylon thread;the torsion/detorsion group underwent torsion induction for 3 h followed by reperfusion for 10 days;the torsion/detorsion+O. europaea group received 300 mg/kg hydro-alcoholic extract of O. europaea 30 min before detorsion, followed by reperfusion for 10 days;and the O. europaea group only received 300 mg/kg hydro-alcoholic extract of O. europaea for 10 days. After the treatment period, blood samples were taken;the levels of estrogen, glutathione peroxidase, superoxide dismutase, and malondialdehyde were assayed. The histological changes, as well as the rate of apoptosis in ovarian tissues, were also carried out by histomorphometric analysis at day 10 post-procedure. Results: Histological comparisons demonstrated a significant detrimental change in the torsion/detorsion group as compared with other groups. The number of pre-antral and antral follicles and corpus luteum was significantly decreased in the torsion/detorsion group compared with the sham group, while treatment with O. europaea could enhance their numbers (P<0.05). The index of apoptosis and the number of atretic body in the ovarian tissue were significantly higher in the torsion/detorsion group compared with the sham group (P<0.05). The concentrations of glutathione peroxidase, estrogen, and superoxide dismutase as well as the mRNA expression of Bcl-2 were considerably diminished in the torsion/detorsion group while they were elevated in the torsion/detorsion+O. europaea group (P<0.05) compared with the torsion/detorsion group. The serum malondialdehyde level and the mRNA expression of Bax were markedly increased during ischemia, while treatment with O. europaea significantly diminished the increased concentrations of malondialdehyde and Bax level in the torsion/detorsion+O. europaea group (P<0.05). Conclusions: O. europaea extract can reduce the degree of tissue damage induced by oxidative stress and apoptosis in the ovary following ovarian ischemia/reperfusion.展开更多
Olive fruits are seriously deteriorated by pre and postharvest damage due to the attack of insects, such as Bactrocera olaea, which strongly alters the quality of olives. Defence response in olive fruits injured both ...Olive fruits are seriously deteriorated by pre and postharvest damage due to the attack of insects, such as Bactrocera olaea, which strongly alters the quality of olives. Defence response in olive fruits injured both by pathogens and by mechanical damages has been associated with the enzyme β-glucosidase, which specifically hydrolyses oleuropein, producing highly reactive aldehyde molecules. In situ detection of ^-glucosidase activity in olive fruit tissues following injury, which simulates Bactrocera oleae punctures, is reported. The assay was performed in two cultivars showing different degrees of susceptibilities to fly infestation. In both cultivars, the histochemical assay for β-glucosidase showed that within 20 min after the injury, a strong ^-glucosidase activity could be observed in the damaged tissues. Thereafter a progressive enzyme inactivation occurred starting from tissues around the boundary of the injury with decrease of the enzyme activity and stopped after 3 h. Whereas the mass of active cells reached a distance of （300±50） μm from the edge of the injury. Biochemical analyses showed that in extracts of the injured fruit, β-glucosidase activity rapidly increased within 20 min from injury, thereafter decreasing and reaching values comparable with those in intact fruits. Following puncture, the oleuropein contents did not change significantly in the high susceptibility cultivar, whereas it rapidly decreased in the cultivar showing low susceptibility. The results strongly suggest that olive fruits susceptible towards fly infestation could be related to the ability of the oleuropein-degrading-β-glucosidase to produce the highly reactive molecules in the damaged tissues. As a consequence of puncture, high level of peroxidase activity was detected. This feature also suggested that this enzyme could play a key role in the defence response against insect injuries.展开更多
Objective:To evaluate the antipiroplasmic activities of methanolic extract of Olea europaea(MOE)and acetonic extract of Acacia laeta(AAL)against Babesia and Theileria parasites in vitro and evaluate the chemotherapeut...Objective:To evaluate the antipiroplasmic activities of methanolic extract of Olea europaea(MOE)and acetonic extract of Acacia laeta(AAL)against Babesia and Theileria parasites in vitro and evaluate the chemotherapeutic effects of these extracts against Babesia(B.)microti in vivo.Methods:Fluorescence assay using SYBR Green 1 nucleic acid stain was used to detect inhibitory effects of the two extracts as well as the combination effects of the two extracts with diminazene aceturate and atovaquone on four Babesia species and Theileria equi in vitro while for in vivo experiments,8-weekold female BALB/c mice were injected intraperitoneally with 1× 107 B.microti-iRBCs and treated orally at a dose of 150 mg/kg of both extracts.Results:The half maximal inhibitory concentration(IC50)values of AAL against B.bovis,B.bigemina,B.divergens,B.caballi,and Theileria equi were lower than those of MOE extracts.Toxicity assay on Madin-Darby bovine kidney,mouse embryonic fibroblast(NIH/3T3),and human foreskin fibroblast cell lines showed that MOE and AAL affected only the viability of Madin-Darby bovine kidney cell line with half maximal effective concentrations(EC50)of(794.7±41.9)and(873.9±17.5)μg/mL,respectively.The oral treatments of MOE and AAL at 150 mg/kg inhibited the growth of B.microti in mice by 80.4% and 64.4%,respectively.The MOE and diminazene aceturate combination showed a higher chemotherapeutic effect than that of monotherapy.Conclusions:MOE and AAL have the potential to be an alternative remedy for treating piroplasmosis.Furthermore,the combination therapy of MOE + DA was more potent against B.microti infection in mice than their monotherapies.展开更多
For the first time, the anatomical structure of the leaf, petiole and fruit of the emerald variety Olea europaea, which grows under the conditions of introduction in the Oltinsay district of the Surkhandarya region of...For the first time, the anatomical structure of the leaf, petiole and fruit of the emerald variety Olea europaea, which grows under the conditions of introduction in the Oltinsay district of the Surkhandarya region of Uzbekistan, was studied. The following characteristic diagnostic features were determined: in the leaf—the dorsiventral type of leaf mesophyll;thick-walled outer walls of the epidermis;slightly sinuous outlines of the cells of the adaxial epidermis, rectilinear-abaxial;hypostomotic leaves;non-submerged stomata of the anomocytic type;thyroid silver-gray and white-fluffy star-shaped squamous-numerous on the adaxial epidermis than on the adaxial;chlorophyll-bearing palisade and spongy parenchyma;closed collateral type of conductive bundles;the most scleralized leaf due to the presence of filamentous scleroids in it (long, thin, resembling fibers);in the petiole—parenchymal-beam type of structure;the location of the angular collenchyma under the epidermis;closed collateral type of conductive bundles;the presence of thick-walled parenchymal cells and the presence of filiformscleroids;pericarp of the fetus consists of a rigid 1-layer exocarp, parenchymal mesocarp, and sclerenchymal endocarp;the presence of scleroids—stony cells and branched sclerenchymal fibers with drops of oil in the parenchymal cells. Diagnostic signs revealed by us reflect xeromorphic of this species. All the signs were compared, and we came to the conclusion that the anatomical signs of the leaf and the fetus can be useful for providing diagnostic signs for distinguishing the studied taxa, can be used in taxonomy, and can serve to identify plant materials.展开更多
Olive(Olea europaea L.)is one of the most important and widely cultivated fruit trees,with high economic,ecological,cultural and scientific value.China began introducing and cultivating olive in the 1960s,and Yunnan P...Olive(Olea europaea L.)is one of the most important and widely cultivated fruit trees,with high economic,ecological,cultural and scientific value.China began introducing and cultivating olive in the 1960s,and Yunnan Province is one of the main growing areas.Improving the cultivation and productivity of this tree crop species is an important challenge.Olive is a typical mycotrophic species and the potential of arbuscular mycorrhizal fungi(AMF)for this plant is well recognized;nevertheless,studies of olive AMF in China are still very limited.Roots and rhizosphere soils of olive were sampled from five representative growing sites in the Yunnan Province of China to investigate the AMF colonization status in the root systems,the AMF community in the olive orchards and the edaphic factors influencing the arbuscular mycorrhizal(AM)parameters.Root samples of olive trees from different growing sites generally showed AMF colonization,suggesting that autochthonous AMF manifest a high efficiency in colonizing the roots of olive plants.The spore density on the five sites ranged from 81.6 to 350 spores per 20 g soil.Twenty-three AMF species from 9 genera were identified in total,and Glomeraceae was the dominant family.The findings of our study suggested a high AMF diversity harbored by olive growing in different areas of the Yunnan Province,Southwestern China.Furthermore,the hyphal colonization in roots positively correlated with soil pH and EC.The arbuscule colonization in olive roots negatively correlated with soil pH,EC,OM,TN,TP and AN.The spore density positively correlated with OM,TN,AN,AP and sand content.Finally,the Shannon index of AMF in the rhizosphere soil positively correlated with the clay content,but negatively correlated with soil pH,TN and silt content.The high diversity of autochthonous AMF in Yunnan is promising for screening AMF isolates for utilization in the efficient cultivation of this crop.展开更多
In order to improve the intensive cultivation and management technology of Olea europaea in the high density orchard,through an analysis on the 3 a growth,yield and orchard construction cost for different varieties an...In order to improve the intensive cultivation and management technology of Olea europaea in the high density orchard,through an analysis on the 3 a growth,yield and orchard construction cost for different varieties and different densities in Gansu Wudu ordinary O.europaea orchard and close O.europaea planting orchard,the appropriate conditions,varieties and density for intensive orchard construction were initially selected.The results showed that the tree height growth in the ordinary orchard was 119%of the tree height growth in the high density orchard,while the ground diameter growth in the ordinary orchard was 112%of the ground diameter growth in the high density orchard,but the yield in the ordinary orchard was only 62%of the yield in the high density orchard;of the 8 new varieties introduced from Spain,"Arbequina","Arbosana"and"Koroneiki"were the appropriate varieties for the intensive orchard construction;the ratio of the ordinary orchard construction cost to the high density orchard construction cost was 1∶6.24.Under high water and fertilizer conditions,the ratio of the ordinary orchard yield to the high density orchard yield was 1∶4.481.Therefore,in the flat land area,using the appropriate intensive cultivars,and integrating the high-tech achievements and advanced management tools,can increase output per unit area,and greatly save manpower and land costs,to achieve the purpose of high quality,high yield and stable production.In the production practice,it is necessary to vigorously promote the intensive cultivation technology of O.europaea.展开更多
基金Supported by Central Finance Forestry Science and Technology Demonstration and Extension Project (E[2023]TG14).
文摘This paper introduced the preparation work before transplanting big olive trees(Olea europaea L.),including key technical measures such as plant excavation,transportation,planting,and post-planting tending.The aim was to provide scientific basis and feasible solutions for adjusting the density of olive trees in Shiyan City,and to assist in the high-quality development of the olive industry.
文摘Olea europaea(Family:Oleaceae)is the characteristic fruit tree of the Mediterranean basin.The oil extracted from the fruit was the essential product,olive oil was used for cooking and as salad oil.It was preferred for its flavor and beneficial health effects.While,low grade oil was used in cosmetics,as lubricants and for soap production.Plant parts were traditionally used in the treatment of diabetes,malaria,hypertension,coughs,asthma,lumbago,rheumatism,kidney problems,urinary tract infections,nose bleeding,for eye infections and to relieve sore throats.About 676 distinct chemical compounds were identified in the Olea europaea,they were included(fatty acids,phenolic compounds,alcohols,volatiles,phospholipids,triterpenic acids,sterols,hydrocarbons,sugars,amino acids,tocopherols,pigments,and many other compounds).Olea europaea extracts and oil possessed many pharmacological activities included cardiovascular,anti-obesity,antidiabetic,inflammatory,analgesic,antioxidant,antimicrobial,antiparasitic,anti-anticancer,immunomodulatory,respiratory,endocrine,reproductive and protective effects.This review discussed the contents,pharmacological,nutritional and therapeutic activities of Olea europaea.
基金This work was supported by China Postdoctoral Science Foundation(2020M670675).
文摘In order to study the possibility of hydroxytyrosol(HT)as a drug,we used SwissADME system to predict ADME of HT and pkCSM system to predict Tox of HT.The results show that hydroxytyrosol meets the Lipinski’s five principles of drug-like properties.With strong efficacy and pharmacological activity,HT has high drug-likeness degree.With good bioavailability,it can be easily absorbed by the gastrointestinal tract,though not absorbed by skin.Hydroxytyrosol has not only a strong potency and pharmacological activity,but also no liver toxicity and skin allergy.Tox data predicts that it has mutagenic potential,which may be the result of overreduction.
文摘Objective: To evaluate the impact of Olea (O.) europaea extract on markers of oxidative stress and apoptosis of ovarian tissues in a rat model of torsion/detorsion-induced ovarian damage. Methods: A total of 28 Wistar female rats were randomly assigned into 4 groups, with 7 rats in each group. The sham group received a 2.5 cm longitudinal incision in the midline part of the abdomen which was then sutured with 5-0 nylon thread;the torsion/detorsion group underwent torsion induction for 3 h followed by reperfusion for 10 days;the torsion/detorsion+O. europaea group received 300 mg/kg hydro-alcoholic extract of O. europaea 30 min before detorsion, followed by reperfusion for 10 days;and the O. europaea group only received 300 mg/kg hydro-alcoholic extract of O. europaea for 10 days. After the treatment period, blood samples were taken;the levels of estrogen, glutathione peroxidase, superoxide dismutase, and malondialdehyde were assayed. The histological changes, as well as the rate of apoptosis in ovarian tissues, were also carried out by histomorphometric analysis at day 10 post-procedure. Results: Histological comparisons demonstrated a significant detrimental change in the torsion/detorsion group as compared with other groups. The number of pre-antral and antral follicles and corpus luteum was significantly decreased in the torsion/detorsion group compared with the sham group, while treatment with O. europaea could enhance their numbers (P<0.05). The index of apoptosis and the number of atretic body in the ovarian tissue were significantly higher in the torsion/detorsion group compared with the sham group (P<0.05). The concentrations of glutathione peroxidase, estrogen, and superoxide dismutase as well as the mRNA expression of Bcl-2 were considerably diminished in the torsion/detorsion group while they were elevated in the torsion/detorsion+O. europaea group (P<0.05) compared with the torsion/detorsion group. The serum malondialdehyde level and the mRNA expression of Bax were markedly increased during ischemia, while treatment with O. europaea significantly diminished the increased concentrations of malondialdehyde and Bax level in the torsion/detorsion+O. europaea group (P<0.05). Conclusions: O. europaea extract can reduce the degree of tissue damage induced by oxidative stress and apoptosis in the ovary following ovarian ischemia/reperfusion.
文摘Olive fruits are seriously deteriorated by pre and postharvest damage due to the attack of insects, such as Bactrocera olaea, which strongly alters the quality of olives. Defence response in olive fruits injured both by pathogens and by mechanical damages has been associated with the enzyme β-glucosidase, which specifically hydrolyses oleuropein, producing highly reactive aldehyde molecules. In situ detection of ^-glucosidase activity in olive fruit tissues following injury, which simulates Bactrocera oleae punctures, is reported. The assay was performed in two cultivars showing different degrees of susceptibilities to fly infestation. In both cultivars, the histochemical assay for β-glucosidase showed that within 20 min after the injury, a strong ^-glucosidase activity could be observed in the damaged tissues. Thereafter a progressive enzyme inactivation occurred starting from tissues around the boundary of the injury with decrease of the enzyme activity and stopped after 3 h. Whereas the mass of active cells reached a distance of （300±50） μm from the edge of the injury. Biochemical analyses showed that in extracts of the injured fruit, β-glucosidase activity rapidly increased within 20 min from injury, thereafter decreasing and reaching values comparable with those in intact fruits. Following puncture, the oleuropein contents did not change significantly in the high susceptibility cultivar, whereas it rapidly decreased in the cultivar showing low susceptibility. The results strongly suggest that olive fruits susceptible towards fly infestation could be related to the ability of the oleuropein-degrading-β-glucosidase to produce the highly reactive molecules in the damaged tissues. As a consequence of puncture, high level of peroxidase activity was detected. This feature also suggested that this enzyme could play a key role in the defence response against insect injuries.
基金supported by the Japan Society for the Promotion of Science(JSPS)(KAKEN Grant Number:18H02337)
文摘Objective:To evaluate the antipiroplasmic activities of methanolic extract of Olea europaea(MOE)and acetonic extract of Acacia laeta(AAL)against Babesia and Theileria parasites in vitro and evaluate the chemotherapeutic effects of these extracts against Babesia(B.)microti in vivo.Methods:Fluorescence assay using SYBR Green 1 nucleic acid stain was used to detect inhibitory effects of the two extracts as well as the combination effects of the two extracts with diminazene aceturate and atovaquone on four Babesia species and Theileria equi in vitro while for in vivo experiments,8-weekold female BALB/c mice were injected intraperitoneally with 1× 107 B.microti-iRBCs and treated orally at a dose of 150 mg/kg of both extracts.Results:The half maximal inhibitory concentration(IC50)values of AAL against B.bovis,B.bigemina,B.divergens,B.caballi,and Theileria equi were lower than those of MOE extracts.Toxicity assay on Madin-Darby bovine kidney,mouse embryonic fibroblast(NIH/3T3),and human foreskin fibroblast cell lines showed that MOE and AAL affected only the viability of Madin-Darby bovine kidney cell line with half maximal effective concentrations(EC50)of(794.7±41.9)and(873.9±17.5)μg/mL,respectively.The oral treatments of MOE and AAL at 150 mg/kg inhibited the growth of B.microti in mice by 80.4% and 64.4%,respectively.The MOE and diminazene aceturate combination showed a higher chemotherapeutic effect than that of monotherapy.Conclusions:MOE and AAL have the potential to be an alternative remedy for treating piroplasmosis.Furthermore,the combination therapy of MOE + DA was more potent against B.microti infection in mice than their monotherapies.
文摘For the first time, the anatomical structure of the leaf, petiole and fruit of the emerald variety Olea europaea, which grows under the conditions of introduction in the Oltinsay district of the Surkhandarya region of Uzbekistan, was studied. The following characteristic diagnostic features were determined: in the leaf—the dorsiventral type of leaf mesophyll;thick-walled outer walls of the epidermis;slightly sinuous outlines of the cells of the adaxial epidermis, rectilinear-abaxial;hypostomotic leaves;non-submerged stomata of the anomocytic type;thyroid silver-gray and white-fluffy star-shaped squamous-numerous on the adaxial epidermis than on the adaxial;chlorophyll-bearing palisade and spongy parenchyma;closed collateral type of conductive bundles;the most scleralized leaf due to the presence of filamentous scleroids in it (long, thin, resembling fibers);in the petiole—parenchymal-beam type of structure;the location of the angular collenchyma under the epidermis;closed collateral type of conductive bundles;the presence of thick-walled parenchymal cells and the presence of filiformscleroids;pericarp of the fetus consists of a rigid 1-layer exocarp, parenchymal mesocarp, and sclerenchymal endocarp;the presence of scleroids—stony cells and branched sclerenchymal fibers with drops of oil in the parenchymal cells. Diagnostic signs revealed by us reflect xeromorphic of this species. All the signs were compared, and we came to the conclusion that the anatomical signs of the leaf and the fetus can be useful for providing diagnostic signs for distinguishing the studied taxa, can be used in taxonomy, and can serve to identify plant materials.
基金supported by the National Natural Science Foundation of China(31760222)State Forestry Administration of China 948 Project“Introduction of Olive Water and Fertilizer Precise Control Technology”(2014-4-23)the“Plant Nutrition and Mycorrhiza Research”Discipline Team Building Project of Yunnan Academy of Forestry and Grassland(LKYTD-2018-6).
文摘Olive(Olea europaea L.)is one of the most important and widely cultivated fruit trees,with high economic,ecological,cultural and scientific value.China began introducing and cultivating olive in the 1960s,and Yunnan Province is one of the main growing areas.Improving the cultivation and productivity of this tree crop species is an important challenge.Olive is a typical mycotrophic species and the potential of arbuscular mycorrhizal fungi(AMF)for this plant is well recognized;nevertheless,studies of olive AMF in China are still very limited.Roots and rhizosphere soils of olive were sampled from five representative growing sites in the Yunnan Province of China to investigate the AMF colonization status in the root systems,the AMF community in the olive orchards and the edaphic factors influencing the arbuscular mycorrhizal(AM)parameters.Root samples of olive trees from different growing sites generally showed AMF colonization,suggesting that autochthonous AMF manifest a high efficiency in colonizing the roots of olive plants.The spore density on the five sites ranged from 81.6 to 350 spores per 20 g soil.Twenty-three AMF species from 9 genera were identified in total,and Glomeraceae was the dominant family.The findings of our study suggested a high AMF diversity harbored by olive growing in different areas of the Yunnan Province,Southwestern China.Furthermore,the hyphal colonization in roots positively correlated with soil pH and EC.The arbuscule colonization in olive roots negatively correlated with soil pH,EC,OM,TN,TP and AN.The spore density positively correlated with OM,TN,AN,AP and sand content.Finally,the Shannon index of AMF in the rhizosphere soil positively correlated with the clay content,but negatively correlated with soil pH,TN and silt content.The high diversity of autochthonous AMF in Yunnan is promising for screening AMF isolates for utilization in the efficient cultivation of this crop.
基金Supported by the Central Finance Forestry Science and Technology Extension Project(2016ZYTG8)
文摘In order to improve the intensive cultivation and management technology of Olea europaea in the high density orchard,through an analysis on the 3 a growth,yield and orchard construction cost for different varieties and different densities in Gansu Wudu ordinary O.europaea orchard and close O.europaea planting orchard,the appropriate conditions,varieties and density for intensive orchard construction were initially selected.The results showed that the tree height growth in the ordinary orchard was 119%of the tree height growth in the high density orchard,while the ground diameter growth in the ordinary orchard was 112%of the ground diameter growth in the high density orchard,but the yield in the ordinary orchard was only 62%of the yield in the high density orchard;of the 8 new varieties introduced from Spain,"Arbequina","Arbosana"and"Koroneiki"were the appropriate varieties for the intensive orchard construction;the ratio of the ordinary orchard construction cost to the high density orchard construction cost was 1∶6.24.Under high water and fertilizer conditions,the ratio of the ordinary orchard yield to the high density orchard yield was 1∶4.481.Therefore,in the flat land area,using the appropriate intensive cultivars,and integrating the high-tech achievements and advanced management tools,can increase output per unit area,and greatly save manpower and land costs,to achieve the purpose of high quality,high yield and stable production.In the production practice,it is necessary to vigorously promote the intensive cultivation technology of O.europaea.
