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Roasted fox nuts (Euryale Ferox L.) contain higher concentration of phenolics, flavonoids, minerals and antioxidants, and exhibit lower Glycemic Index (GI) in human subjects 被引量:2
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作者 Marrium Liaquat Imran Pasha +1 位作者 Muhammad Ahsin Abdullah Salik 《Food Production, Processing and Nutrition》 2022年第1期1-12,共12页
Fox nut (Euryale ferox) is categorized among superior quality foods because of its highly nutritious profile. It also serves as a good protein source for vegetarian diets. However, it is not widely consumed, due to so... Fox nut (Euryale ferox) is categorized among superior quality foods because of its highly nutritious profile. It also serves as a good protein source for vegetarian diets. However, it is not widely consumed, due to soggy texture and bland flavor as compared to other nuts. In this study, we used roasting and seasoning to enhance its consumer acceptance. Furthermore, we have also compared biochemical, nutritional and physical properties of the roasted and unroasted fox nuts;and their Glycemic Index (GI) in human subjects. Results showed that roasting significantly (P ≤ 0.05) improved the minerals content (from 0.58 to 0.66%), protein content (from 11.40 to 14.57%), total phenolics (from 346.02 to 470.62 mg GAE/100 g), flavonoids (from 4.15 to 4.43 mg CE/g), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (from 48.54 to 79.13% inhibition) and Ferric Reducing Antioxidant Power (from 662.46 to 957.14 μmol FeSO4/g). Consumer acceptance was evaluated with and without seasoning;both salt and spice seasoned roasted nuts had greater overall acceptability as compared to plain roasted and unroasted nuts. Fox nuts also reported low GI (37%) in human subjects (ethical approval #1772/01-06-2020). Thus, roasted fox nuts can be consumed as nutrient rich and low GI snack;not only as a component of healthy diet but can also be served to patients with metabolic disorders like obesity and diabetes. 展开更多
关键词 euryale ferox seeds Fox nuts Roasting Nutritious snack and low glycemic index foods
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芡实提取物对克氏原螯虾生长、抗氧化能力及免疫功能的影响 被引量:8
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作者 闫戈 顾晨 +2 位作者 王承鑫 李世平 魏万红 《淡水渔业》 CSCD 北大核心 2022年第1期83-89,共7页
为探究芡实(Euryale ferox)提取物对克氏原螯虾(Procambarus clarkii)生长免疫的影响,本实验按照饲喂饲料芡实提取物的添加浓度分为对照组(0 g/kg)、0.5%芡实组(5 g/kg)、1%芡实组(10 g/kg)和2%芡实组(20 g/kg),连续投喂克氏原螯虾28 d... 为探究芡实(Euryale ferox)提取物对克氏原螯虾(Procambarus clarkii)生长免疫的影响,本实验按照饲喂饲料芡实提取物的添加浓度分为对照组(0 g/kg)、0.5%芡实组(5 g/kg)、1%芡实组(10 g/kg)和2%芡实组(20 g/kg),连续投喂克氏原螯虾28 d,实验结束后测定克氏原螯虾的生长参数和嗜水气单胞菌攻毒后的存活率,并分析了抗氧化酶活、免疫相关酶活和相关基因表达情况。结果显示:芡实提取物对克氏原螯虾的生长和嗜水气单胞菌攻毒后的存活率没有影响。与对照组相比,0.5%芡实饲喂显著增加了克氏原螯虾血清中的酸性磷酸酶(ACP)和碱性磷酸酶(AKP)活性,而1%和2%芡实血清中的ACP活性显著降低,2%芡实组克氏原螯虾血清中的SOD活性显著增加。在肝胰腺中,0.5%芡实组克氏原螯虾的ACP活性也显著增加,但其它酶活没有显著变化。荧光定量PCR的结果显示,2%芡实显著增加了克氏原螯虾CypA和GPxs mRNA的表达,而Astacidin mRNA的表达在各组之间均无显著差异。综上结果表明,芡实提取物能在一定程度上增强克氏原螯虾的抗氧化能力和免疫力,而不影响其生长。 展开更多
关键词 克氏原螯虾(Procambarus clarkii) 芡实(euryale ferox) 药用植物 免疫增强剂
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芡实SBE3基因克隆及其生物信息学分析
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作者 王前 严洪楷 +4 位作者 邵永芳 景宗慧 刘莉成 鲍锞 吴啟南 《分子植物育种》 CAS 北大核心 2023年第19期6346-6355,共10页
芡实的主要组成成分是淀粉,淀粉分支酶SBE3能够调控支链淀粉的合成,为了深入研究芡实SBE3基因的功能与调控机制,本研究克隆出芡实淀粉分支酶SBE3基因并对其进行相关生物信息学分析。通过提取芡实种子总RNA,采用RT-PCR方法克隆芡实SBE3基... 芡实的主要组成成分是淀粉,淀粉分支酶SBE3能够调控支链淀粉的合成,为了深入研究芡实SBE3基因的功能与调控机制,本研究克隆出芡实淀粉分支酶SBE3基因并对其进行相关生物信息学分析。通过提取芡实种子总RNA,采用RT-PCR方法克隆芡实SBE3基因,利用软件和在线网站对该基因及其编码氨基酸序列的理化性质、蛋白质结构、系统发育树和启动子等进行生物信息学分析与预测。获得EfSBE3基因全长2830bp,开放阅读框长2736bp,编码911个氨基酸;预测其为亲水性蛋白,无跨膜区,无信号肽。系统进化分析表明该序列与同科植物睡莲亲缘关系最近,启动子分析共预测出10类顺式作用元件。本研究结果可为芡实分子育种提供理论依据。 展开更多
关键词 芡实(euryale ferox Salisb.) SBE3 基因克隆 生物信息学分析
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