Comments on eurytrematosis in Brazil and the possibility of human infection

The manuscript "Eurytrematosis: An emerging and neglected disease in South Brazil" discusses some aspects of Eurytrema sp. fluke as an animal pathogen and based in some aspects of the parasitism in cattle and the life cycle of Eurytrema sp. Authors suggest the possibility of human infection, once there is no research on this subject in Brazil. In human cases reported, the mechanism of infection was not disclosed, so it keeps the discussion opened. Although we focused on animal eurytrematosis, we speculated the possibility of human infection by Eurytrema sp. in Brazil, but after all, the only way to determine it, would be a study searching for people infected through coprological or serological tests.

利用RAPD技术对胰阔盘吸虫和腔阔盘吸虫基因组DNA扩增的实验

利用RAPD技术，对胰阔金吸虫和胚阔盘吸虫两种吸虫的基因组DNA进行了扩增比较。对25种随机引物进行了筛选，得到一些能够对胰阔盘吸虫基因组DNA扩增的引物．并且利用三种引物对胰阔盘吸虫群体内个体间基因组DNA多态性进行了检测，以期能够利用RAPD技术对胰阔盘吸虫群体遗传结构进行研究。

山羊腔阔盘吸虫的形态学观察和分子鉴定

为了从形态学和分子水平对山羊腔阔盘吸虫(Eurytrema coelomaticum)的种类鉴定提供依据,对采集自广东佛山发病山羊胰脏的阔盘吸虫在形态学观察的基础上,抽提虫体基因组DNA,通过PCR方法对虫体核糖体18SrRNA基因和线粒体cox1基因进行扩增,将产物与pMD18-T载体连接,转化到大肠埃希菌DH5α感受态细胞进行克隆、测序和序列分析。结果获得虫体18SrRNA基因的大小为1 922bp,线粒体cox 1基因大小为444bp。序列分析显示,获得的18SrRNA基因与GenBank收录的腔阔盘吸虫(E.coelomaticum,登录号为DQ401035)同源性高达99%;线粒体cox1基因与GenBank收录的胰阔盘吸虫(E.pancreaticum,登录号为KC535544)的同源性仅为90%。研究结果从分子水平证明采集自广东佛山山羊体的阔盘吸虫为腔阔盘吸虫(E.coelomaticum),对山羊阔盘吸虫病的防控有指导意义。