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以EVENS综合征为主要表现的高龄B细胞淋巴瘤1例
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作者 邢明泉 葛洪峰 《菏泽医学专科学校学报》 2024年第1期83-84,共2页
患者,男,89岁,因“发现血小板减少2天”于2022年12月5日来院就诊。血常规:WBC:5.10×10^(9)/L,Hb:84g/L,PLT:33×10^(9)/L,总胆红素36.3μmol/L,间接胆红素25.1μmol/L,LDH:1123 U/L,血清铁蛋白>1675.56 ng/m L,网织红细胞计... 患者,男,89岁,因“发现血小板减少2天”于2022年12月5日来院就诊。血常规:WBC:5.10×10^(9)/L,Hb:84g/L,PLT:33×10^(9)/L,总胆红素36.3μmol/L,间接胆红素25.1μmol/L,LDH:1123 U/L,血清铁蛋白>1675.56 ng/m L,网织红细胞计数:0.155,网织红细胞比值:5.32%。 展开更多
关键词 B细胞淋巴瘤 EVENS综合征 免疫组织化学
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Spatio-temporal evolution analysis of landscape pattern and habitat quality in the Qinghai Province section of the Yellow River Basin from 2000 to 2022 based on InVEST model
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作者 ZHENG Guoqiang Li Cunxiu +3 位作者 LI Runjie LUO Jing FAN Chunxia ZHU Hailing 《Journal of Arid Land》 SCIE CSCD 2024年第9期1183-1196,共14页
Habitat quality is an important indicator for evaluating the quality of ecosystem.The Qinghai Province section of the Yellow River Basin plays an important role in the ecological protection of the upper reaches of the... Habitat quality is an important indicator for evaluating the quality of ecosystem.The Qinghai Province section of the Yellow River Basin plays an important role in the ecological protection of the upper reaches of the Yellow River Basin.To comprehensively analysis the alterations of habitat quality in the Qinghai Province section of the Yellow River Basin,this study utilized the Integrated Valuation of Ecosystem Services and Tradeoffs(InVEST)model to calculate the habitat quality index and analyze the spatio-temporal variation characteristics of habitat quality in the study area from 2000 to 2022,and calculated seven landscape pattern indices(number of patches,patch density,largest patch index(LPI),landscape shape index(LSI),contagion index(CONTAG),Shannon diversity index,and Shannon evenness index)to research the variation of landscape pattern in the study area.The results showed that the number of patches,patch density,LPI,LSI,Shannon diversity index,and Shannon evenness index increased from 2000 to 2022,while the CONTAG decreased,indicating that the landscape pattern in the Qinghai Province section of the Yellow River Basin changed in the direction of distribution fragmentation,shape complexity,and heterogeneity.The average value of the habitat quality index in the Qinghai Province section of the Yellow River Basin from 2000 to 2022 was 0.90.Based on the value of habitat quality index,we divided the level of habitat quality into five categories:lower(0.00-0.20),low(0.20-0.40),moderate(0.40-0.60),high(0.60-0.80),and higher(0.80-1.00).Most areas were at the higher habitat quality level.The lower habitat quality patches were mainly distributed in Longyang Gorge and Yellow River-Huangshui River Valley.From 2000 to 2022,the habitat quality in most areas was stable;the increase areas were mainly distributed in Guinan County,while the decrease areas were mainly distributed in Xining City,Maqen County,Xinghai County,Qumarleb County,and Darlag County.To show the extent of habitat quality variation,we calculated Sen index.The results showed that the higher habitat quality area had a decrease trending,while other categories had an increasing tendency,and the decreasing was faster than increasing.The research results provide scientific guidance for promoting ecological protection and high-quality development in the Qinghai Province section of the Yellow River Basin. 展开更多
关键词 InVEST model landscape pattern index habitat quality largest patch index landscape shape index Shannon evenness index
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Combination therapy using evening primrose oil and electrical stimulation to improve nerve function following a crush injury of sciatic nerve in male rats 被引量:7
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作者 Omid Badri Parviz Shahabi +4 位作者 Jalal Abdolalizadeh Mohammad Reza Alipour Hadi Veladi Mehdi Farhoudi Mohsen Sharif Zak 《Neural Regeneration Research》 SCIE CAS CSCD 2017年第3期458-463,共6页
Peripheral nerve injuries with a poor prognosis are common.Evening primrose oil(EPO) has beneficial biological effects and immunomodulatory properties.Since electrical activity plays a major role in neural regenerat... Peripheral nerve injuries with a poor prognosis are common.Evening primrose oil(EPO) has beneficial biological effects and immunomodulatory properties.Since electrical activity plays a major role in neural regeneration,the present study investigated the effects of electrical stimulation(ES),combined with evening primrose oil(EPO),on sciatic nerve function after a crush injury in rats.In anesthetized rats,the sciatic nerve was crushed using small haemostatic forceps followed by ES and/or EPO treatment for 4 weeks.Functional recovery of the sciatic nerve was assessed using the sciatic functional index.Histopathological changes of gastrocnemius muscle atrophy were investigated by light microscopy.Electrophysiological changes were assessed by the nerve conduction velocity of sciatic nerves.Immunohistochemistry was used to determine the remyelination of the sciatic nerve following the interventions.EPO + ES,EPO,and ES obviously improved sciatic nerve function assessed by the sciatic functional index and nerve conduction velocity of the sciatic nerve at 28 days after operation.Expression of the peripheral nerve remyelination marker,protein zero(P0),was increased in the treatment groups at 28 days after operation.Muscle atrophy severity was decreased significantly while the nerve conduction velocity was increased significantly in rats with sciatic nerve injury in the injury + EPO + ES group than in the EPO or ES group.Totally speaking,the combined use of EPO and ES may produce an improving effect on the function of sciatic nerves injured by a crush.The increased expression of P0 may have contributed to improving the functional effects of combination therapy with EPO and ES as well as the electrophysiological and histopathological features of the injured peripheral nerve. 展开更多
关键词 nerve regeneration peripheral nerve injury sciatic nerve injury evening primrose oil electrical stimulation sciatic functional index cuff electrode neural regeneration
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独立林雪夜——Stopping by Woods on a Snowy Evening一诗的翻译和欣赏
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作者 贾荣江 《襄樊职业技术学院学报》 2002年第4期63-65,共3页
分析了《StoppingbyWoodsOnaSnowyEvening》一诗的原义 ,并用适当的汉语和诗歌形式将原义表达出来。说明了此诗所描写的一种典型环境对揭示诗中人物性格所起的作用。
关键词 《独立林雪夜》 诗歌欣赏 典型环境 诗歌翻译 英译文 人物性格 《Stopping by WOODS on a SNOWY evening
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International Wedding & Evening Dress Week Inaugurated in Chaozhou
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作者 Linda Zhao 《China's Foreign Trade》 2017年第6期58-58,共1页
On November 10, China (Chaozhou) International Wedding & Evening Dress Week, sponsored by the China National Garment Association and the China Fashion Association, was inaugurated in Chaozhou.
关键词 International Wedding evening Dress Week Inaugurated Chaozhou
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Stopping by Woods on a Snowy Evening两个中译本对比赏析
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作者 李婷婷 《文教资料》 2018年第10期22-23,共2页
罗伯特·弗罗斯特是20世纪美国最受欢迎的诗人之一,曾四度获得普利策奖。其代表作之一"Stopping by Woods on a Snowy Evening"已被译成多种版本来供中国的广大读者阅读与欣赏。本文通过文学文体学理论,从词汇表达、句法结构、修... 罗伯特·弗罗斯特是20世纪美国最受欢迎的诗人之一,曾四度获得普利策奖。其代表作之一"Stopping by Woods on a Snowy Evening"已被译成多种版本来供中国的广大读者阅读与欣赏。本文通过文学文体学理论,从词汇表达、句法结构、修辞特征、衔接与语境四个方面对比与欣赏余光中和顾子欣对该诗的译本,揭示文学文体学理论对评价诗歌文体翻译的指导意义。 展开更多
关键词 罗伯特·弗罗斯特 STOPPING by WOODS on a SNOWY evening 文学文体学 中译
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A Blue Sky to Be Shared by Humankind——A Special Evening Party Titled “Green Olympics, Energy-Saving & Environment Protection”
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《China & The World Cultural Exchange》 2002年第1期21-21,共1页
关键词 BE A Blue Sky to Be Shared by Humankind A Special evening Party Titled ENERGY-SAVING Environment Protection Green Olympics
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A Critical Discourse Analysis of the 315 Evening Party
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作者 胡喜喜 《海外英语》 2014年第23期231-232,269,共3页
According to Norman Fairclough's three-dimensional model of discourse analysis, this paper studies the transcription of the 315 evening party to explore the ideologies hidden behind. Based on the analysis, this pa... According to Norman Fairclough's three-dimensional model of discourse analysis, this paper studies the transcription of the 315 evening party to explore the ideologies hidden behind. Based on the analysis, this paper concludes that the coexistence of the legal conscience and the ethnics and their competing relationship are the underlying causes of the emergence of the 315 evening party. 展开更多
关键词 315 evening part CRITICAL DISCOURSE analysis LEGAL
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40~65岁人群睡眠时型与血脂异常的关联性分析 被引量:3
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作者 梁晓仙 杨瑾 +6 位作者 金菊珍 周静 胡瑾 盖云 丁晓云 汪俊华 王子云 《中国全科医学》 北大核心 2023年第34期4277-4282,共6页
背景 中老年人的晚睡行为可能影响其血脂代谢,但二者的关联性仍需深入分析。目的 探讨不同性别、中心性肥胖、睡前加餐和吸烟人群睡眠时型与血脂异常的关联性。方法 对2022年3—8月在福泉市第一人民医院体检中心体检的40~65岁人群开展... 背景 中老年人的晚睡行为可能影响其血脂代谢,但二者的关联性仍需深入分析。目的 探讨不同性别、中心性肥胖、睡前加餐和吸烟人群睡眠时型与血脂异常的关联性。方法 对2022年3—8月在福泉市第一人民医院体检中心体检的40~65岁人群开展问卷调查(n=697),收集研究对象的一般资料和睡眠情况,采用“清晨型-夜晚型量表”的单项问题来评价睡眠时型。采用非条件二分类Logistic回归模型评估睡眠时型与血脂异常患病风险的关联,在总人群中以性别、中心性肥胖、睡前加餐进行分层分析,在男性人群中以是否吸烟进行分层分析。采用敏感性分析排除夜班工作的影响。结果 纳入体检者中,睡眠时型为清晨型偏好393人(56.4%)、夜晚型偏好304人(43.6%),检出血脂异常334人(47.9%)。剔除夜班人群后的非条件二分类Logistic回归分析结果显示,睡眠时型是血脂异常的影响因素[OR(95%CI)=1.54(1.10,2.16)](P<0.05),夜晚型人群患高TG血症、低HDL-C血症的风险高于清晨型人群[OR(95%CI)分别为1.48(1.04,2.12)、1.79(1.18,2.72)](P<0.05)。在总人群中进行分层分析结果显示:在男性人群和中心性肥胖人群中,夜晚型人群患低HDL-C血症的风险分别是清晨型的1.80倍[95%CI为(1.12,2.91)](P<0.05)和1.73倍[95%CI为(1.02,2.81)](P<0.05);在睡前加餐人群中,夜晚型人群患高TG血症的风险是清晨型的3.43倍[95%CI为(1.30,8.99)](P<0.05),在女性人群、非中心性肥胖人群中睡眠时型对血脂异常及其各指标均无明显影响(P>0.05)。在男性人群中以吸烟分层,结果显示:在吸烟的男性人群中,夜晚型人群患低HDL-C血症的风险是清晨型的1.83倍[95%CI为(1.03,3.26)](P<0.05),在吸烟和不吸烟的男性人群中,睡眠时型对高TC血症、高TG血症、高LDL-C血症和非HDL-C异常均无明显影响(P>0.05)。结论 在40~65岁人群中,夜晚型睡眠偏好可能是血脂异常的危险因素。不同性别、是否为中心性肥胖、睡前是否加餐、是否吸烟人群的睡眠时型与血脂异常之间的关联性可能存在一定差异。 展开更多
关键词 睡眠时型 昼夜节律 清晨型-夜晚型 血脂异常
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早-晚偏好型新手驾驶员危险感知的特征分析
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作者 陈雯 马蓉 王华容 《人类工效学》 2023年第1期27-31,43,共6页
目的为了探讨早晚偏好型新手驾驶员的危险感知特点,为交通安全教育提供启示和参考。方法采用2(早-晚型偏好类型:早间型vs晚间型)×2(实验时间:早间vs晚间)混合实验设计。运用早晚型量表(MEQ),从南通某高校随机选择持有驾照的新手大... 目的为了探讨早晚偏好型新手驾驶员的危险感知特点,为交通安全教育提供启示和参考。方法采用2(早-晚型偏好类型:早间型vs晚间型)×2(实验时间:早间vs晚间)混合实验设计。运用早晚型量表(MEQ),从南通某高校随机选择持有驾照的新手大学生。采用经过汉化修订的英国驾驶理论测试中的危险感知任务检测被试危险感知水平,同时运用Biopac多导生理仪,数据结果采用SPSS20.0进行分析。结果早间型被试危险感知得分显著低于晚间型被试。驾驶员晚间实验时的呼吸频率显著高于早间,早间型驾驶员在晚间实验时的肌电值显著高于其在早间实验时的肌电值。结论驾驶员危险感知过程中的肌电、呼吸受时间的影响。不同偏好新手驾驶员的的危险感知水平受到时间的影响,晚间型驾驶员危险感知能力稍高于早间型驾驶员。 展开更多
关键词 驾驶行为 交通安全 早晚型偏好 驾驶员 危险感知 生理变化 事故预防
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清晨型与夜晚型评定量表的信度与效度 被引量:43
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作者 张斌 郝彦利 荣润国 《中国行为医学科学》 CSCD 2006年第9期856-858,共3页
目的完成清晨型与夜晚型评定量表(MEQ)中文版的引进工作。方法首先进行MEQ量表项目的翻译定稿,然后应用中文版评定163名健康受试者的日周期倾向,并通过两周睡眠记录表记录他们的睡眠习惯。根据测量的结果,进行量表信度与效度检验... 目的完成清晨型与夜晚型评定量表(MEQ)中文版的引进工作。方法首先进行MEQ量表项目的翻译定稿,然后应用中文版评定163名健康受试者的日周期倾向,并通过两周睡眠记录表记录他们的睡眠习惯。根据测量的结果,进行量表信度与效度检验,并试图寻找其划界点。结果MEQ量表中文版的19个条目被确定为两个因子:睡眠时相因子和最佳表现时间因子。中文版的划界点:绝对清晨型:70-86、中度清晨型:63-69、中间型:50-62、中度夜晚型:43-49、绝对夜晚型:16-42。中文版的Chronbachα系数为0.701-0.738、Spearman-Brown分半信度为0.584-0.697、重测信度为0.638-0.831,均达到了心理测量学可接受的水平。结论MEQ量表中文版有良好的心理测量学特征,新划界点可以有效地区分清晨型和夜晚型。 展开更多
关键词 清晨型与夜晚型评定量表 信度 效度
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清晨型/夜晚型睡眠者的社会心理学特征 被引量:6
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作者 张斌 郝彦利 荣润国 《中国心理卫生杂志》 CSSCI CSCD 北大核心 2006年第9期621-624,共4页
目的:了解清晨型/夜晚型睡眠者的社会心理学特征。方法:筛选出163名健康睡眠者,包括26例清晨型、108例中间型、29例夜晚型。在面对面的临床访谈中,了解他们的社会人口学特征。使用两周睡眠记录表了解他们的睡眠习惯,使用中文版清晨型/... 目的:了解清晨型/夜晚型睡眠者的社会心理学特征。方法:筛选出163名健康睡眠者,包括26例清晨型、108例中间型、29例夜晚型。在面对面的临床访谈中,了解他们的社会人口学特征。使用两周睡眠记录表了解他们的睡眠习惯,使用中文版清晨型/夜晚型量表评定他们的日周期类型,使用A型人格量表和艾森克人格量表了解他们的人格类型,使用自评问卷了解他们的焦虑抑郁水平。结果:清晨型\中间型\夜晚型睡眠者的社会人口学特征和精神活性物质应用情况相似。在艾森克人格量表评定中,清晨型比中间型和夜晚型有更低的内外向分量表分(清晨型:8.00±3.33、中间型:9.97±3.61、夜晚型:10.28±3.74,F=3.54,P=0.03),而夜晚型比清晨型和中间型有更高的说谎分量表分(清晨型:7.62±3.24、中间型:8.54±3.25、夜晚型:10.24±2.73,F=4.60,P=0.01)。其他人格和心理评定未见差别。结论:夜晚型比清晨型具有更明显的外向性。 展开更多
关键词 睡眠 清晨型/夜晚型 横断面调查 社会心理学特征
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日周期类型与心理健康 被引量:10
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作者 宋晶晶 郑涌 《心理科学进展》 CSSCI CSCD 北大核心 2014年第9期1446-1455,共10页
个体间表现出的昼夜节律差异现象可以从日周期类型上进行分类,包括偏爱早睡早起的清晨型,偏爱晚睡晚起的夜晚型以及处于两者之间的中间型。已有研究表明,夜晚型与心境障碍、注意缺陷多动障碍和进食障碍等心理疾病有关,而日周期类型与心... 个体间表现出的昼夜节律差异现象可以从日周期类型上进行分类,包括偏爱早睡早起的清晨型,偏爱晚睡晚起的夜晚型以及处于两者之间的中间型。已有研究表明,夜晚型与心境障碍、注意缺陷多动障碍和进食障碍等心理疾病有关,而日周期类型与心理健康的关系可能受到基因、社会时差、人格等因素的影响。未来应改进研究设计和测量方法,深入考察日周期类型与心理健康的关系及其作用机制,以及在我国文化背景下推进日周期类型与心理健康的关系研究。 展开更多
关键词 日周期类型 清晨型 夜晚型 心理健康
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Landscape pattern and diversity of natural secondary forests in the eastern mountainous region, northeast China:A case study of Mao'ershan region in Heilongjiang Province
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作者 李淑娟 隋玉正 +2 位作者 冯海清 王凤友 李玉文 《Journal of Forestry Research》 SCIE CAS CSCD 2004年第3期181-186,共6页
Mao抏rshan region is a representative natural secondary forested region in the eastern mountainous region, north-east of China. Under the support of ARC/INFO, the landscape pattern and landscape diversity of Maoerhsha... Mao抏rshan region is a representative natural secondary forested region in the eastern mountainous region, north-east of China. Under the support of ARC/INFO, the landscape pattern and landscape diversity of Maoerhshan region were studied by combining the forest type map (1:10000), which was drawn from the aerial photographs (1999), field investigation and land utilization map (1:10000). The selected indices included patch number, patch size, patch density index, richness index, dominance index, evenness index and diversity index. The results showed that the landscape dominant forest type in Mao抏rshan region was softwood broad-leaved forest. In all landscape types, the average patch area of natural secondary for-ests was bigger than that of artificial forest. The patch density index of each landscape formed in artificial forest was higher than that of natural secondary forest. The landscape diversity index and landscape evenness index of natural forest were highest, the landscape heterogeneity was also, but the landscape dominance was lower. In natural forest, the control effects of landscape elements on landscape-structure, function and its change were weakened. The artificial forest was on the contrary. 展开更多
关键词 Landscape pattern Landscape diversity DOMINANCE EVENNESS Diversity index Maoershan region Heilongjiang
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这个从句中的that起什么作用?
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作者 肖云枢 《语言教育》 2002年第7期45-45,共1页
[问][387]新修订的《大学英语》(上海外语教育出版社,1997年12月第2版)精读第三册第8单元中有一个句子(p.175):Attitudes to wards daydreamingare changing in much the same way thatattitudes towards night dreaming havechanged.请... [问][387]新修订的《大学英语》(上海外语教育出版社,1997年12月第2版)精读第三册第8单元中有一个句子(p.175):Attitudes to wards daydreamingare changing in much the same way thatattitudes towards night dreaming havechanged.请问that在这里起什么作用呢? 展开更多
关键词 that 《大学英语》 night 上海外语教育 关系代词 大学外语 THINGS FRIEND 最大力量 evening
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张学良诗二首译介
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作者 汪榕培 《语言教育》 2001年第11期12-12,共1页
关键词 不怕死 evening
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中小学教师作息型态、调节模式导向与职业倦怠的关系:工作拖延、工作投入的中介效应 被引量:4
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作者 王蕾 陈毅文 杨莎 《中国健康心理学杂志》 2020年第2期294-300,共7页
目的:研究了中小学教师作息型态、调节模式导向对职业倦怠的影响,考查了工作拖延、工作投入在其中起到的中介作用。方法:选取北京、山西、河南3个省的424名中小学教师为研究对象,采用问卷调查的方式研究了中小学教师作息型态、调节模式... 目的:研究了中小学教师作息型态、调节模式导向对职业倦怠的影响,考查了工作拖延、工作投入在其中起到的中介作用。方法:选取北京、山西、河南3个省的424名中小学教师为研究对象,采用问卷调查的方式研究了中小学教师作息型态、调节模式导向对职业倦怠的影响,探讨了工作拖延、工作投入在其中起到的中介作用。结果:作息型态可以负向预测工作拖延(β=-0.22,P<0.001)。运动模式可以负向预测工作拖延(β=-0.49,P<0.001),可以正向预测工作投入(β=0.71,P<0.001);评估模式可以正向预测工作拖延(β=0.27,P<0.001)。工作拖延可以正向预测职业倦怠(β=0.24,P<0.001)。工作投入可以负向预测职业倦怠(β=-0.72,P<0.001)。结论:中小学教师作息型态可以通过工作拖延对职业倦怠发挥作用;运动模式可以通过工作投入和工作拖延对职业倦怠发挥作用;评估模式可以通过工作拖延对职业倦怠发挥作用。 展开更多
关键词 中小学教师 职业倦怠 工作投入 工作拖延 作息型态 调节模式导向
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Supportive therapies for prevention of hepatocellular carcinoma recurrence and preservation of liver function 被引量:4
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作者 Taro Takami Takahiro Yamasaki +3 位作者 Issei Saeki Toshihiko Matsumoto Yutaka Suehiro Isao Sakaida 《World Journal of Gastroenterology》 SCIE CAS 2016年第32期7252-7263,共12页
Hepatocellular carcinoma(HCC) is one of the deadliest cancers in the world and is associated with a high risk of recurrence. The development of a wide range of new therapies is therefore essential. In this study, from... Hepatocellular carcinoma(HCC) is one of the deadliest cancers in the world and is associated with a high risk of recurrence. The development of a wide range of new therapies is therefore essential. In this study, from the perspective of supportive therapy for the prevention of HCC recurrence and preservation of liver function in HCC patients, we surveyed a variety of different therapeutic agents. We show that branched chain amino acids(BCAA) supplementation and late evening snack with BCAA, strategies that address issues of protein-energy malnutrition, are important for liver cirrhotic patients with HCC. For chemoprevention of HCC recurrence, we show that viral control after radical treatment is important. We also reviewed the therapeutic potential of antiviral drugs, sorafenib, peretinoin, iron chelators. Sorafenib is a kinase inhibitor and a standard therapy in the treatment of advanced HCC. Peretinoin is a vitamin A-like molecule that targets the retinoid nuclear receptor to induce apoptosis and inhibit tumor growth in HCC cells. Iron chelators, such as deferoxamine and deferasirox, act to prevent cancer cell growth. These chelators may have potential as combination therapies in conjunction with peretinoin. Finally, we review the potential inhibitory effect of bone marrow cells on hepatocarcinogenesis. 展开更多
关键词 Hepatocellular carcinoma Liver cirrhosis Branched-chain amino acids Late evening SNACK Iron CHELATORS Bone MARROW cells
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Forest Community Structure and Species Diversity of Eight Forest Communities in Wenchang City
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作者 洪仁辉 薛杨 +3 位作者 王小燕 李敦禧 林之盼 宿少锋 《Agricultural Science & Technology》 CAS 2016年第9期2137-2141,共5页
The research took different forest communities in Wenchang Satellite Launch Center as the research object and selected 8 forests to explore species diversity, evenness, and dominance of forests. The results showed tha... The research took different forest communities in Wenchang Satellite Launch Center as the research object and selected 8 forests to explore species diversity, evenness, and dominance of forests. The results showed that species diversity tended to be volatile upon forest communities; semi-mangrove species was the richest, as high as 2.38, followed by natural secondary forest and mixed forests. Plant evenness from high to low was as follows: herbaceous layer〉shrub layer〉tree layer. Natural secondary forest was higher in canopy density, and fewer in herbaceous plants; diversity and evenness were lower than shrub layer. Mangrove forests held lower dominance, and dominance showed insignificant differences among species. 展开更多
关键词 Different forest types Diversity EVENNESS Degree of dominance
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Sequencing a Juglans regia×J.microcarpa hybrid yields high-quality genome assemblies of parental species 被引量:8
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作者 Tingting Zhu Le Wang +15 位作者 Frank M.You Juan C.Rodriguez Karin R.Deal Limin Chen Jie Li Sandeep Chakraborty Bipin Balan Cai-Zhong Jiang Patrick J.Brown Charles ALeslie Mallikarjuna KAradhya Abhaya M.Dandekar Patrick E.McGuire Daniel Kluepfel Jan Dvorak Ming-Cheng Luo 《Horticulture Research》 SCIE 2019年第1期1271-1286,共16页
Members of the genus Juglans are monecious wind-pollinated trees in the family Juglandaceae with highly heterozygous genomes,which greatly complicates genome sequence assembly.The genomes of interspecific hybrids are ... Members of the genus Juglans are monecious wind-pollinated trees in the family Juglandaceae with highly heterozygous genomes,which greatly complicates genome sequence assembly.The genomes of interspecific hybrids are usually comprised of haploid genomes of parental species.We exploited this attribute of interspecific hybrids to avoid heterozygosity and sequenced an interspecific hybrid Juglans microcarpa×J.regia using a novel combination of single-molecule sequencing and optical genome mapping technologies.The resulting assemblies of both genomes were remarkably complete including chromosome termini and centromere regions.Chromosome termini consisted of arrays of telomeric repeats about 8 kb long and heterochromatic subtelomeric regions about 10 kb long.The centromeres consisted of arrays of a centromere-specific Gypsy retrotransposon and most contained genes,many of them transcribed.Juglans genomes evolved by a whole-genome-duplication dating back to the Cretaceous-Paleogene boundary and consist of two subgenomes,which were fractionated by numerous short gene deletions evenly distributed along the length of the chromosomes.Fractionation was shown to be asymmetric with one subgenome exhibiting greater gene loss than the other.The asymmetry of the process is ongoing and mirrors an asymmetry in gene expression between the subgenomes.Given the importance of J.microcarpa×J.regia hybrids as potential walnut rootstocks,we catalogued disease resistance genes in the parental genomes and studied their chromosomal distribution.We also estimated the molecular clock rates for woody perennials and deployed them in estimating divergence times of Juglans genomes and those of other woody perennials. 展开更多
关键词 JUGLANS PARENTAL evenly
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