秀珍菇枯萎病的病原菌鉴定及污染源分析

从感染枯萎病的秀珍菇(Pleurotus pulmonarius)样本中分离纯化病原菌,经柯赫氏法则验证TLH07菌株为病原菌,该菌株会导致菇体出现枯萎、菌盖凹陷等特征。经菌落形态、显微特征及16S rRNA、gyrB基因序列分析鉴定病原菌为美洲爱文菌(Ewingella americana)。病原菌对秀珍菇菌丝生长具有抑制作用。污染源调查发现,菌袋栽培料表层、内层、底层,棉塞和水源的细菌污染率分别为40%、6%、0%、17%和10%。接种操作不当可能是秀珍菇枯萎病发生的原因。本研究结果可为秀珍菇枯萎病病害的防控提供参考。

Evaluation of plant growth promoting potential of four rhizobacterial species for indigenous system

The aim of this work was to see whether Pseudomonas putida NWU12, Pseudomonas fluorescence NWU65, Vibrio fluvialis NWU37 and Ewingella americana NWU59 are beneficial to plants and are able to promote plant growth and development when inoculated as plant growth-promoting rhizobacteria (PGPR). The four rhizobacteria were tested in vitro for PGPR activities and on spinach and pepper in pot experiments. The inoculants are all positive for ammonia (NH3 ), catalase, hydrogen cyanide (HCN), phosphate solubilization and siderophore production. Among the inoculants, E. americana NWU59 is oxidase negative. P. putida NWU12 and P. fluorescence NWU65 are producing indole-3-acetic acid (IAA). The inoculants exhibit some PGPR activities and thus tested in the screen-house. Treatments are control (water) and the four inoculants. Rhizobacterial inoculants increase spinach (17.14%-21.43%) and pepper (15.0%-37.5%) plant heights over the control. Such inoculants have the potential of improving plant yield components and may be used as biofertilizer.

1例美洲爱文菌感染患儿抗感染治疗策略分析

目的:探讨罕见病原菌的抗感染治疗策略。方法:临床药师通过了解病原菌种属、致病力及常用治疗药物选择,结合1例美洲爱文菌感染患儿病理生理情况,为临床制定抗感染治疗方案提供建议。结果:临床药师协助医师及时、准确地选用抗菌药物、调整治疗方案,患儿病情好转出院。结论:临床药师从病原菌出发,从药物选择和机体对药物反应角度协助医师制定抗感染方案,发挥临床药师专业优势。