OBJECTIVE： To explore the pathogenesis of dampness syndrome by detecting the changes of inter- leukin-2 （IL-2） and interleukin-8 （IL-8） levels. METHODS： Female Sprague Dawley rats were divided randomly into five...OBJECTIVE： To explore the pathogenesis of dampness syndrome by detecting the changes of inter- leukin-2 （IL-2） and interleukin-8 （IL-8） levels. METHODS： Female Sprague Dawley rats were divided randomly into five groups according to the random number table： a normal group （Group Ⅰ ）, an external dampness group （Group Ⅱ ）, an internal dampness group （Group Ⅲ）, and an external and internal dampness group （Group Ⅳ）. Twenty days after the model made, IL-2 and IL-8 levels were detected by radioimmunoassay method. RESULTS： The IL-2 and IL-8 levels among groups were significant （F=3.102, P〈0.05; F=2.657, P〈0.05, respectively）. The level of IL-2 in Group Ⅱ and Group Ⅲ were higher than that in Group Ⅰ （P〈0.05, P〈0.01, respectively）, especially higher in the Group Ⅲ compared with Group Ⅱ （P〈0.05）. The level of IL-8 in Group Ⅲ were higher than those in Group Ⅰ, Group Ⅱ and GroupⅣ （P〈0.05, P〈0.01, P〈 0.05, respectively）. In the Group Ⅲ, the 24-hour water and body weight were higher than that in the Group Ⅱ （all P〈0.05）, and spontaneous movement frequency was higher than those in Group Ⅱ and GroupⅣ （P〈0.05）. CONCLUSION： Immune activation and inflammatory reaction might be easily caused by external danpness other than internal dampness.展开更多
基金Supported by National Natural Science Foundation of China (No. 81072806)"Eleventh Five-year Plan" for Sci &Tech Research of China (No. 2009ZX10005-016)
文摘OBJECTIVE： To explore the pathogenesis of dampness syndrome by detecting the changes of inter- leukin-2 （IL-2） and interleukin-8 （IL-8） levels. METHODS： Female Sprague Dawley rats were divided randomly into five groups according to the random number table： a normal group （Group Ⅰ ）, an external dampness group （Group Ⅱ ）, an internal dampness group （Group Ⅲ）, and an external and internal dampness group （Group Ⅳ）. Twenty days after the model made, IL-2 and IL-8 levels were detected by radioimmunoassay method. RESULTS： The IL-2 and IL-8 levels among groups were significant （F=3.102, P〈0.05; F=2.657, P〈0.05, respectively）. The level of IL-2 in Group Ⅱ and Group Ⅲ were higher than that in Group Ⅰ （P〈0.05, P〈0.01, respectively）, especially higher in the Group Ⅲ compared with Group Ⅱ （P〈0.05）. The level of IL-8 in Group Ⅲ were higher than those in Group Ⅰ, Group Ⅱ and GroupⅣ （P〈0.05, P〈0.01, P〈 0.05, respectively）. In the Group Ⅲ, the 24-hour water and body weight were higher than that in the Group Ⅱ （all P〈0.05）, and spontaneous movement frequency was higher than those in Group Ⅱ and GroupⅣ （P〈0.05）. CONCLUSION： Immune activation and inflammatory reaction might be easily caused by external danpness other than internal dampness.
