Objective： To study the pathologic features of plasmacytoid transitional cell carcinoma of the bladder, and to analyze the diagnostic features, criteria for differential diagnosis and the clinical significance of the...Objective： To study the pathologic features of plasmacytoid transitional cell carcinoma of the bladder, and to analyze the diagnostic features, criteria for differential diagnosis and the clinical significance of the tumor. Methods： Two cases of bladder plasmacytoid transitional cell carcinoma were studied. Routine paraffin sections with HE staining, Pap smear and immunohistochemistry by S-P method were observed under a light microscope. Pathological and clinical data were analyzed by comparison with early reported cases in literatures. Results： A characteristic feature of this tumor was of deep invasion in the lamina propria and/or muscularis propria, in addition to the component of carcinoma in situ in the mucosa, when tumors were diagnosed. The histological pattern and cytological features showed similarity to a plasmacytoid tumor. The tumor cells were strongly positive for AE1/AE3, CEA and CK18. The prognosis appeared to be worse than ordinary transitional cell carcinoma. Conclusion： The plasmacytoid transitional cell carcinoma of bladder is rare but has typical pathological, immunohistological and clinical features. Pathologists should be aware of this kind of primary tumor of bladder.展开更多
Background:Squamous cell carcinoma(SCC)of the bladder is common in many regions around the world.Prognosis is very poor,as most cases are diagnosed at an advanced stage due to a lack of affordable and valid screening ...Background:Squamous cell carcinoma(SCC)of the bladder is common in many regions around the world.Prognosis is very poor,as most cases are diagnosed at an advanced stage due to a lack of affordable and valid screening markers for this type of cancer.The diagnostic accuracy of urinary nuclear matrix protein-22(NMP22),telomerase activity,and CD44 were evaluated in urine samples of patients with bladder SCC.Materials and methods:We conducted a case-control study comprised of 60 consecutive newly diagnosed bladder SCC patients diagnosed by cystoscopy and histopathological examination,and controls were 60 outpatients with benign urologic conditions and healthy clinic visitors.Urine samples collected from each subject underwent testing for NMP22,telomerase activity,and CD44.Descriptive and correlational statistical analysis of cases and controls were carried out and receiver operating characteristic curve analysis was used to determine optimal cut-off points for the three assays.Results:Area under the curve was calculated at 0.96,0.93,and 0.62 for NMP22,telomerase,and CD44,respectively.Urine levels of NMP22 and telomerase activity were significantly higher in the SCC group compared to controls(p<0.001).Urine CD44 levels were not significantly higher in the SCC group compared to controls(p=0.111).The overall sensitivity of NMP22,telomerase,and CD44 was 96.7%,87%,and 45%,respectively,while the specificity was 85%,88.6%,and 86.7%,respectively.Conclusions:Urinary telomerase activity,followed by NMP22 urine levels,showed high diagnostic yield and could hold potential promise as urinary biomarkers for the diagnosis of bladder SCC.展开更多
背景与目的:目前膀胱癌疗效和监测的主要方法是膀胱镜和尿细胞学检查,前者为侵入性检查,令患者感到不适;后者虽无创且特异性高,但敏感性太低,且受主观因素影响大。本研究拟对中、英文有关比较荧光原位杂交(fluorescence in situ hybridi...背景与目的:目前膀胱癌疗效和监测的主要方法是膀胱镜和尿细胞学检查,前者为侵入性检查,令患者感到不适;后者虽无创且特异性高,但敏感性太低,且受主观因素影响大。本研究拟对中、英文有关比较荧光原位杂交(fluorescence in situ hybridization,FISH)和尿细胞学检查诊断膀胱癌研究的结果进行系统分析,以明确FISH对膀胱癌的诊断意义。方法:采用Cochrane系统评价方法,MEDLINE(1966年1月～2008年6月)、EMBASE(1988年1月～2008年6月)、Cochrane图书馆、中国生物医学期刊文献数据库(CMCC,1979年～2008年6月)、CNKI数字图书馆(1979年1月～2008年6月)进行有关FISH和尿细胞学检查诊断膀胱癌文献的检索、质量评价和资料提取,采用MetaDiSc1.4软件进行Meta分析。结果:共检索到相关研究242篇,排除230篇,符合纳入标准12篇进入Meta分析,涉及研究对象3430例。异质性检验提示无阈值效应,但存在其它原因导致的异质性。按随机效应模型进行Meta分析,FISH和尿细胞学诊断膀胱癌的准确度指标敏感度、特异度、阳性似然比、阴性似然比以及诊断优势比等汇总及95%CI分别为74%(71%～77%)vs.57%(54%～61%)、88%(86%～90%)vs.85%(83%～87%)、6.18(3.56～10.73)vs.4.15(2.78～6.20)、0.29(0.19～0.45)vs.0.51(0.41～0.63)及24.17(9.33～62.64)vs.9.59(5.91～15.57)。FISH和尿脱落细胞学检查的敏感度随肿瘤分级、分期的升高而增高。综合受试者工作特征曲线下面积分别为0.8938、0.8247,Q*值分别为0.7847、0.7226。结论:FISH诊断膀胱癌的准确度较高,但对高分期的敏感度较细胞学低,目前尚不能取代传统的尿细胞学检查,但可作为膀胱癌术前诊断、术后监测和随访的指标。展开更多
文摘Objective： To study the pathologic features of plasmacytoid transitional cell carcinoma of the bladder, and to analyze the diagnostic features, criteria for differential diagnosis and the clinical significance of the tumor. Methods： Two cases of bladder plasmacytoid transitional cell carcinoma were studied. Routine paraffin sections with HE staining, Pap smear and immunohistochemistry by S-P method were observed under a light microscope. Pathological and clinical data were analyzed by comparison with early reported cases in literatures. Results： A characteristic feature of this tumor was of deep invasion in the lamina propria and/or muscularis propria, in addition to the component of carcinoma in situ in the mucosa, when tumors were diagnosed. The histological pattern and cytological features showed similarity to a plasmacytoid tumor. The tumor cells were strongly positive for AE1/AE3, CEA and CK18. The prognosis appeared to be worse than ordinary transitional cell carcinoma. Conclusion： The plasmacytoid transitional cell carcinoma of bladder is rare but has typical pathological, immunohistological and clinical features. Pathologists should be aware of this kind of primary tumor of bladder.
基金supported by a Senate Pilot Grant by the University of California San Diego.
文摘Background:Squamous cell carcinoma(SCC)of the bladder is common in many regions around the world.Prognosis is very poor,as most cases are diagnosed at an advanced stage due to a lack of affordable and valid screening markers for this type of cancer.The diagnostic accuracy of urinary nuclear matrix protein-22(NMP22),telomerase activity,and CD44 were evaluated in urine samples of patients with bladder SCC.Materials and methods:We conducted a case-control study comprised of 60 consecutive newly diagnosed bladder SCC patients diagnosed by cystoscopy and histopathological examination,and controls were 60 outpatients with benign urologic conditions and healthy clinic visitors.Urine samples collected from each subject underwent testing for NMP22,telomerase activity,and CD44.Descriptive and correlational statistical analysis of cases and controls were carried out and receiver operating characteristic curve analysis was used to determine optimal cut-off points for the three assays.Results:Area under the curve was calculated at 0.96,0.93,and 0.62 for NMP22,telomerase,and CD44,respectively.Urine levels of NMP22 and telomerase activity were significantly higher in the SCC group compared to controls(p<0.001).Urine CD44 levels were not significantly higher in the SCC group compared to controls(p=0.111).The overall sensitivity of NMP22,telomerase,and CD44 was 96.7%,87%,and 45%,respectively,while the specificity was 85%,88.6%,and 86.7%,respectively.Conclusions:Urinary telomerase activity,followed by NMP22 urine levels,showed high diagnostic yield and could hold potential promise as urinary biomarkers for the diagnosis of bladder SCC.
文摘背景与目的:目前膀胱癌疗效和监测的主要方法是膀胱镜和尿细胞学检查,前者为侵入性检查,令患者感到不适;后者虽无创且特异性高,但敏感性太低,且受主观因素影响大。本研究拟对中、英文有关比较荧光原位杂交(fluorescence in situ hybridization,FISH)和尿细胞学检查诊断膀胱癌研究的结果进行系统分析,以明确FISH对膀胱癌的诊断意义。方法:采用Cochrane系统评价方法,MEDLINE(1966年1月～2008年6月)、EMBASE(1988年1月～2008年6月)、Cochrane图书馆、中国生物医学期刊文献数据库(CMCC,1979年～2008年6月)、CNKI数字图书馆(1979年1月～2008年6月)进行有关FISH和尿细胞学检查诊断膀胱癌文献的检索、质量评价和资料提取,采用MetaDiSc1.4软件进行Meta分析。结果:共检索到相关研究242篇,排除230篇,符合纳入标准12篇进入Meta分析,涉及研究对象3430例。异质性检验提示无阈值效应,但存在其它原因导致的异质性。按随机效应模型进行Meta分析,FISH和尿细胞学诊断膀胱癌的准确度指标敏感度、特异度、阳性似然比、阴性似然比以及诊断优势比等汇总及95%CI分别为74%(71%～77%)vs.57%(54%～61%)、88%(86%～90%)vs.85%(83%～87%)、6.18(3.56～10.73)vs.4.15(2.78～6.20)、0.29(0.19～0.45)vs.0.51(0.41～0.63)及24.17(9.33～62.64)vs.9.59(5.91～15.57)。FISH和尿脱落细胞学检查的敏感度随肿瘤分级、分期的升高而增高。综合受试者工作特征曲线下面积分别为0.8938、0.8247,Q*值分别为0.7847、0.7226。结论:FISH诊断膀胱癌的准确度较高,但对高分期的敏感度较细胞学低,目前尚不能取代传统的尿细胞学检查,但可作为膀胱癌术前诊断、术后监测和随访的指标。
