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A、B、J亚群禽白血病病毒混合感染的鉴定及gp85基因序列分析 被引量:1
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作者 窦俊峰 汪最 +5 位作者 李丽 卢琴 金鑫鑫 凌小淳 罗青平 翟新国 《中国畜牧兽医》 CSCD 北大核心 2024年第1期302-311,共10页
【目的】了解湖北地区禽白血病病毒(Avian leukosis virus, ALV)亚群之间混合感染及其gp85基因遗传变异情况,为进一步研究ALV混合感染的流行病学提供参考。【方法】对来自湖北宜昌疑似感染ALV的一只病死鸡进行病理剖检,无菌采集病变组... 【目的】了解湖北地区禽白血病病毒(Avian leukosis virus, ALV)亚群之间混合感染及其gp85基因遗传变异情况,为进一步研究ALV混合感染的流行病学提供参考。【方法】对来自湖北宜昌疑似感染ALV的一只病死鸡进行病理剖检,无菌采集病变组织接种DF-1细胞进行病毒分离,通过PCR、ELISA及间接免疫荧光试验(IFA)等方法进行亚群鉴定,进一步对分离株gp85基因的相似性和变异情况进行分析。【结果】剖检结果可见病死鸡肾脏、脾脏肿大,肺脏出血,表面可见灰白色肿瘤结节;组织匀浆接种DF-1细胞后,ELISA检测发现细胞上清P27抗原呈阳性;IFA检测发现,感染细胞里有特异性绿色荧光;PCR鉴定能同时扩增出A(692 bp)、B(847 bp)和J(545 bp)亚群的特异性条带。鉴定结果表明,从该病死鸡中分离到A、B和J亚群ALV毒株,分别命名为HBYC2022-A、HBYC2022-B和HBYC2022-J。分离株gp85基因核苷酸相似性分析显示,HBYC2022-A与江苏株JS-A1201相似性最高,为99.6%;HBYC2022-B与江苏株JS-B1204相似性最高,为99.7%;而HBYC2022-J与黑龙江株PK19FA01、广西株GX20YL12 J及安徽株AHaq02相似性均为100%。此外,分离株gp85基因高突变区域(hr1、hr2)氨基酸序列并未出现特殊点突变。【结论】鸡群存在A、B和J不同亚群ALV的混合感染情况,提示国内养禽场需提高警惕并加强针对ALV各亚群的流行病学调查。 展开更多
关键词 禽白血病病毒(ALV) 分离鉴定 混合感染 GP85基因 序列分析
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J、K亚群禽白血病病毒的分离和囊膜蛋白(gp85)基因序列分析
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作者 周钊灿 游广炬 +4 位作者 杨金易 苏晓娜 高丽 王永强 郑世军 《中国兽医杂志》 CAS 北大核心 2024年第4期1-10,共10页
为了解现阶段我国禽白血病病毒(ALV)的流行特征和演化趋势,本试验于2021—2022年从国内某鸡场采集蛋清ALV衣壳蛋白(p27)抗原阳性的鸡血浆,接种鸡胚成纤维细胞(DF-1)分离病毒并进行亚群鉴定;采用聚合酶链式反应(PCR)技术扩增分离株的囊... 为了解现阶段我国禽白血病病毒(ALV)的流行特征和演化趋势,本试验于2021—2022年从国内某鸡场采集蛋清ALV衣壳蛋白(p27)抗原阳性的鸡血浆,接种鸡胚成纤维细胞(DF-1)分离病毒并进行亚群鉴定;采用聚合酶链式反应(PCR)技术扩增分离株的囊膜蛋白(gp85)基因片段,并对其进行测序,将基因序列与ALV不同亚群毒株进行比对和遗传进化分析;选取氨基酸变化差异较大的分离毒株进行间接免疫荧光鉴定。结果显示,共分离到7株ALV毒株(3株J亚群,4株K亚群),ALV-J分离毒株CAU4932、CAU4860和CAU2259的gp 85基因片段长度为918 bp,编码306个氨基酸,分离毒株之间gp 85基因核苷酸同源性为90.5%~95.2%,gp85蛋白氨基酸同源性为95.1%~99.3%;ALV-K分离毒株CAU7049、CAU5006、CAU7176和CAU7168的gp 85基因片段长度为1008 bp,编码336个氨基酸,分离毒株之间gp 85基因核苷酸同源性为89.4%~92.3%,gp85蛋白氨基酸同源性为94.0%~99.7%。gp85蛋白氨基酸序列同源性比对发现,分离株gp85蛋白氨基酸序列均会发生突变,但高变区hr1和hr2所占比例较多,证实了gp85的高变性。在gp85蛋白B细胞抗原表位中存在部分位点的氨基酸突变,可能导致gp85蛋白抗原性发生变化。间接免疫荧光鉴定结果显示,5株分离毒株均可在DF-1细胞内复制并存在可被抗体识别的p27抗原表位。结果表明,本试验分离的7株ALV毒株是国内ALV-J和ALV-K流行毒株的突变株,不仅丰富了ALV基因组库资源,且为后续研究ALV的遗传变异特征和流行趋势等提供参考依据。 展开更多
关键词 禽白血病病毒 J亚群 K亚群 gp 85基因 序列比较
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回归温度和时间对深冷变形7A85铝合金显微组织、力学性能和腐蚀行为的影响
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作者 王当 张文学 +3 位作者 易幼平 黄始全 何海林 张京京 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2024年第2期392-407,共16页
为了平衡合金的强度、塑性与耐蚀性,在不同回归温度和时间条件下对深冷变形7A85铝合金的显微组织和综合性能进行研究。结果表明,在较低回归温度和时间的回归再时效(RRA)制度和T6(120℃,24h)制度下,合金具有较高的强度,但耐蚀性较差。对... 为了平衡合金的强度、塑性与耐蚀性,在不同回归温度和时间条件下对深冷变形7A85铝合金的显微组织和综合性能进行研究。结果表明,在较低回归温度和时间的回归再时效(RRA)制度和T6(120℃,24h)制度下,合金具有较高的强度,但耐蚀性较差。对于RRA,在一定范围内增加回归温度和时间会降低合金的强度,但合金的电导率和耐蚀性显著提高。然而,过度地增加回归温度和时间使合金的耐蚀性降低。相同的回归温度下,随着回归时间的延长,合金的伸长率先减小后增大。与三向深冷变形7A85铝合金的最佳匹配的制度为RRA180-0.5((120℃,24 h)+(180℃,0.5 h)+(120℃,24 h))。 展开更多
关键词 深冷变形7A85铝合金 回归温度 回归时间 强度 耐蚀性 最佳制度
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基于低温制备色层法的溶解尾气中氪-85纯化装置与方法
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作者 张震 和文龙 +7 位作者 张罡 秦来来 张轩 姜国涛 龚伟 李连顺 杨廷贵 王健 《当代化工》 CAS 2024年第7期1525-1529,1534,共6页
^(85)Kr是一种非常有价值的放射性气态核素。目前,我国^(85)Kr气体基本依赖进口满足市场需求。纯化作为^(85)Kr产业提取-纯化一体化生产系统的重要技术,是获得高纯度^(85)Kr的重要手段,因此在进行纯化热实验以及正式的工业生产前需要进... ^(85)Kr是一种非常有价值的放射性气态核素。目前,我国^(85)Kr气体基本依赖进口满足市场需求。纯化作为^(85)Kr产业提取-纯化一体化生产系统的重要技术,是获得高纯度^(85)Kr的重要手段,因此在进行纯化热实验以及正式的工业生产前需要进行冷实验确定纯化过程的重要参数。搭建了基于低温制备色层法的纯化冷实验台架,研究了色层柱填料和温度对氪气纯化效果的影响,结果表明:椰壳活性炭具有比5A分子筛更好的纯化效果;采用椰壳活性炭填充色层柱,色层柱温度降低至-40℃时,氪气可以和氮气、氧气等杂质完全分离,氪气的纯化效果较好,产品气中氪-84的峰显著高于氮气和氧气,氪气的纯度大幅提高。 展开更多
关键词 ^(85)Kr 氪气 纯化 低温制备色层法
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Expert Experience and Data-Driven Based Hybrid Fault Diagnosis for High-SpeedWire Rod Finishing Mills 被引量:1
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作者 Cunsong Wang Ningze Tang +3 位作者 Quanling Zhang Lixin Gao Haichen Yin Hao Peng 《Computer Modeling in Engineering & Sciences》 SCIE EI 2024年第2期1827-1847,共21页
The reliable operation of high-speed wire rod finishing mills is crucial in the steel production enterprise.As complex system-level equipment,it is difficult for high-speed wire rod finishing mills to realize fault lo... The reliable operation of high-speed wire rod finishing mills is crucial in the steel production enterprise.As complex system-level equipment,it is difficult for high-speed wire rod finishing mills to realize fault location and real-time monitoring.To solve the above problems,an expert experience and data-driven-based hybrid fault diagnosis method for high-speed wire rod finishing mills is proposed in this paper.First,based on its mechanical structure,time and frequency domain analysis are improved in fault feature extraction.The approach of combining virtual value,peak value with kurtosis value index,is adopted in time domain analysis.Speed adjustment and side frequency analysis are proposed in frequency domain analysis to obtain accurate component characteristic frequency and its corresponding sideband.Then,according to time and frequency domain characteristics,fault location based on expert experience is proposed to get an accurate fault result.Finally,the proposed method is implemented in the equipment intelligent diagnosis system.By taking an equipment fault on site,for example,the effectiveness of the proposed method is illustrated in the system. 展开更多
关键词 High-speed wire rod finishing mills expert experience DATA-DRIVEN fault diagnosis
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热处理后7A85合金的组织和拉伸性能研究
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作者 童广泽 刘闪光 +2 位作者 刘东洋 高文理 王卫凯 《热加工工艺》 北大核心 2024年第8期16-18,22,共4页
7A85合金是我国自主研发的Al-Zn-Mg-Cu合金,被广泛应用于航空航天领域。研究了热处理后7A85合金的组织和拉伸性能,这对加速并拓宽7A85合金的工业化应用具有十分重要的意义。结果表明,热处理之后7A85合金的晶粒为长条形且存在很多亚晶。... 7A85合金是我国自主研发的Al-Zn-Mg-Cu合金,被广泛应用于航空航天领域。研究了热处理后7A85合金的组织和拉伸性能,这对加速并拓宽7A85合金的工业化应用具有十分重要的意义。结果表明,热处理之后7A85合金的晶粒为长条形且存在很多亚晶。热处理后的7A85合金由GP区和η'相协同强化。微米级的晶界第二相主要由Al、Zn、Mg、Cu元素在晶界处偏聚形成,Zr元素没有在晶界处发生偏聚形成晶界第二相。纳米级的晶界主要由Mg和Zn偏聚形成,在某些第二相中含有Cu。热处理后7A85合金的抗拉强度为596 MPa,屈服强度为572 MPa,伸长率为10.6%。 展开更多
关键词 7A85合金 晶粒 GP区 η'相 第二相 拉伸性能
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肝细胞癌组织核孔蛋白85(NUP85)高表达与免疫细胞浸润及预后相关性分析
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作者 刘新宇 丁泽邦 +2 位作者 吴焕 马俊杰 陆进 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2024年第6期508-519,共12页
目的 探究核孔蛋白85(NUP85)在肝细胞癌(HCC)中的表达意义及免疫相关性分析。方法 综合利用多种在线数据库分析NUP85在HCC中的mRNA表达、蛋白表达以及突变情况,并进行预后诊断价值分析;采用单细胞测序数据及肿瘤免疫评估资源(TIMER)和... 目的 探究核孔蛋白85(NUP85)在肝细胞癌(HCC)中的表达意义及免疫相关性分析。方法 综合利用多种在线数据库分析NUP85在HCC中的mRNA表达、蛋白表达以及突变情况,并进行预后诊断价值分析;采用单细胞测序数据及肿瘤免疫评估资源(TIMER)和基因表达谱交互作用分析2021(GEPIA2021)数据库分析NUP85的免疫相关性;利用基因组癌症分析(GSCA)和临床生信之家分析NUP85的药物敏感性;通过肝细胞癌综合分子数据库(HCCDB)筛选NUP85在HCC中的共表达基因,并利用R语言“limma包”分析NUP85及其相关基因的相关性;再利用R语言“clusterProfiler包”分析NUP85及其相关基因的基因本体论(GO)功能注释、京都基因与基因组百科全书(KEGG)以及基因集富集分析(GSEA);利用临床生信之家对NUP85及其相关基因进行列线图和预后风险评分模型的构建。结果 NUP85的mRNA和蛋白质在HCC中表达上调,在不同分期和分级均高表达,不利于患者预后;而其在HCC样本中突变率是19%,显著影响患者的总生存期(OS)、疾病特异性生存(DSS)以及无进展生存(PFS),且其在巨噬细胞、 B细胞、 T细胞等多种免疫细胞中高表达并与多种免疫细胞浸润水平呈正相关;此外,NUP85的表达与米尔达美替尼(PD0325901)、维莫非尼的结构类似物(PLX4720)和瑞法替尼(PD0325901)等多用药物具有显著相关性。NUP85及其共表达基因的GO功能主要富集在细胞器裂变、核分裂和染色体分离等,KEGG通路主要富集在细胞周期和运动蛋白等,并显著不利于HCC患者OS,且对HCC患者1年、 3年和5年OS预后诊断的ROC曲线下面积AUC均大于0.7。结论 NUP85高表达HCC患者预后差,并与多种免疫细胞和药物相关,可作为HCC诊断、治疗和预后的生物标志物。 展开更多
关键词 核孔蛋白85(NUP85) 肝细胞癌 单细胞分析 免疫浸润 生物信息学
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Mg_(85)Ni_(15)减反层对玻璃衬底上VO_(2)薄膜光电性能的影响
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作者 王坤 王麒雯 +1 位作者 刘初蕾 康朝阳 《真空科学与技术学报》 CAS CSCD 北大核心 2024年第7期577-582,共6页
采用脉冲激光沉积技术,在非晶玻璃衬底上生长单斜晶相的二氧化钒(M-VO_(2))薄膜,并研究了Mg_(85)Ni_(15)减反层对M-VO_(2)薄膜性能的影响。实验结果表明:在非晶玻璃衬底上制备的M-VO_(2)薄膜具有单一取向且纯度较高,并具有良好的光电热... 采用脉冲激光沉积技术,在非晶玻璃衬底上生长单斜晶相的二氧化钒(M-VO_(2))薄膜,并研究了Mg_(85)Ni_(15)减反层对M-VO_(2)薄膜性能的影响。实验结果表明:在非晶玻璃衬底上制备的M-VO_(2)薄膜具有单一取向且纯度较高,并具有良好的光电热致转变性能。Mg_(85)Ni_(15)作为减反层,在没有特别降低电学性能的基础上,提高了薄膜的可见光透过率和太阳能调节率。通过对减反层厚度的优化发现:在减反层厚度为60 nm时,相变温度最低,且热滞宽度最窄为6℃。此工作的开展有利于推进VO_(2)薄膜在智能窗方面的应用。 展开更多
关键词 脉冲激光沉积 VO_(2) 薄膜 Mg_(85)Ni_(15) 减反层 光电性能
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lnc85通过调节CDC42的表达促进肝癌细胞增殖
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作者 宋振宇 杨丽超 +4 位作者 廖舟翔 张起 温莎 黄雪静 何敏 《广西医科大学学报》 CAS 2024年第2期186-192,共7页
目的:探讨长链非编码RNA RP11-85G21.1(lnc85)对肝癌细胞增殖的作用及可能机制。方法:RNA全转录组测序结合实时荧光定量PCR(RT-qPCR)筛选验证肝癌中差异高表达的lnc85;利用lnc85过表达/空载慢病毒感染细胞,构建lnc85过表达肝癌细胞株(OE... 目的:探讨长链非编码RNA RP11-85G21.1(lnc85)对肝癌细胞增殖的作用及可能机制。方法:RNA全转录组测序结合实时荧光定量PCR(RT-qPCR)筛选验证肝癌中差异高表达的lnc85;利用lnc85过表达/空载慢病毒感染细胞,构建lnc85过表达肝癌细胞株(OE)和对照细胞(control);RNA下拉实验(RNA pull-down)联合质谱筛选lnc85互作蛋白并进行蛋白免疫印迹(western blotting)验证;MTT实验、细胞克隆形成实验分析lnc85过表达对细胞增殖的影响;生物信息学分析lnc85互作蛋白表达水平及对生存的影响。结果:RNA全转录组测序发现,与对照相比,lnc85在肝癌患者血浆外泌体中明显高表达(P<0.001),RT-qPCR验证lnc85在3种肝癌细胞中显著升高(P<0.01,P<0.001);RNA pull-down联合质谱分析发现CDC42为lnc85的互作蛋白,RT-qPCR证实CDC42水平在肝癌细胞显著升高(P<0.001);且lnc85过表达的肝癌细胞中,CDC42蛋白水平显著上调(P<0.001),细胞增殖能力显著增强(P<0.01);生物信息学分析发现,肝癌患者CDC42的表达水平显著高于健康对照(P<0.001),并随着肿瘤等级的增高而增高;高表达CDC42的肝癌患者预后不良(P<0.001)。结论:lnc85在肝癌中高表达,可能通过靶向调节CDC42的表达促进肝癌细胞的增殖。 展开更多
关键词 肝癌 长链非编码RNA RP11-85G21.1 细胞分裂周期蛋白42 RNA下拉实验 细胞增殖
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国外后处理厂氪-85管理实践及其启示
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作者 刘建权 曹龙浩 +2 位作者 赵学延 韦萌 任丽丽 《广东化工》 CAS 2024年第14期59-61,80,共4页
由于氪-85是后处理厂排放的放射性活度最高的同位素,且能造成全球较高的集体剂量,20世纪70年代起,以美国为代表的国家都对氪-85分离整备技术进行了科学探索和工程实践,发展出了以低温蒸馏为代表的技术路线,除在氪分离工艺、氪的长期贮... 由于氪-85是后处理厂排放的放射性活度最高的同位素,且能造成全球较高的集体剂量,20世纪70年代起,以美国为代表的国家都对氪-85分离整备技术进行了科学探索和工程实践,发展出了以低温蒸馏为代表的技术路线,除在氪分离工艺、氪的长期贮存中都遇到了安全问题。与此同时,由于氪-85放射性毒性低、对公众个人有效剂量的贡献很低、氪分离整备工程化存在一定安全问题且投资巨大,法国、英国的后处理厂将几乎全部的氪-85以气载流出物形式排放到环境中进行稀释扩散。另外,尽管氪-85是一种应用较为广泛的同位素,但其市场规模远小于后处理厂氪-85的年排放量。综上,建议我国参考法、英等国的氪-85管理实践选择稀释扩散方案对后处理厂氪-85进行管理。 展开更多
关键词 氪-85 后处理厂 管理方案 分离整备 稀释扩散
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Finding biomarkers of experience in animals
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作者 Sarah Babington Alan J.Tilbrook +9 位作者 Shane K.Maloney Jill N.Fernandes Tamsyn M.Crowley Luoyang Ding Archa H.Fox Song Zhang Elise A.Kho Daniel Cozzolino Timothy J.Mahony Dominique Blache 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第3期925-944,共20页
At a time when there is a growing public interest in animal welfare,it is critical to have objective means to assess the way that an animal experiences a situation.Objectivity is critical to ensure appropriate animal ... At a time when there is a growing public interest in animal welfare,it is critical to have objective means to assess the way that an animal experiences a situation.Objectivity is critical to ensure appropriate animal welfare outcomes.Existing behavioural,physiological,and neurobiological indicators that are used to assess animal welfare can verify the absence of extremely negative outcomes.But welfare is more than an absence of negative outcomes and an appropriate indicator should reflect the full spectrum of experience of an animal,from negative to positive.In this review,we draw from the knowledge of human biomedical science to propose a list of candidate biological markers(biomarkers)that should reflect the experiential state of non-human animals.The proposed biomarkers can be classified on their main function as endocrine,oxidative stress,non-coding molecular,and thermobiological markers.We also discuss practical challenges that must be addressed before any of these biomarkers can become useful to assess the experience of an animal in real-life. 展开更多
关键词 Animal experience Animal welfare BIOMARKER STRESS Welfare assessment
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Acceptance and Experience of HIV-Infected Adolescents in Two Hospitals in Yaoundé
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作者 Isabelle Mekone Nkwele Nelly Kamgaing +7 位作者 Pierrette Amanda Emah Manga Jeannette Épée Ngoué Hélène Kamo Sélangai Claude Audrey Meguieze Nsoh Ndeh-Fofang Serge Tchoukoua Jean-Baptiste Fotso Djemo Paul Koki Ndombo 《Open Journal of Pediatrics》 2024年第2期435-443,共9页
Introduction: Access to antiretroviral drugs has improved the survival of children infected with the Human Immunodeficiency Virus (HIV). As they reach adolescence, they are confronted with various constraints related ... Introduction: Access to antiretroviral drugs has improved the survival of children infected with the Human Immunodeficiency Virus (HIV). As they reach adolescence, they are confronted with various constraints related to the infection and its treatment, in addition to those of the growth period they are going through. The main aim of the study was to assess the acceptance and describe the experience of HIV infection by infected adolescents but also to investigate the factors associated with good acceptance and a positive experience. Methodology: The cross-sectional analytic study concerned HIV-infected adolescents aged 15 to 19 followed up at the Chantal Biya Foundation-Mother and Child Centre (CME-FCB) and the Yaoundé University Hospital Centre (CHUY) between February 2020 and June 2020. The study saw participants complete a questionnaire containing socio-demographic data and assessing acceptance and experience with the infection. Data analysis was accomplished using Epi info software version 7.2.2.6. Results: One hundred and thirteen HIV-infected adolescents were included in the study. The sex ratio was 0.68 and the mean age was 17 years. More than half of the adolescents had a good acceptance and positive experience with the infection. Related factors were the adolescent’s perception of good health and participation in an association with other infected adolescents. Conclusion: Emphasizing the psychological and educational follow-up of infected adolescents and encouraging their participation in associations for adolescents living with HIV could reduce the consequences of poor acceptance and ensure a better transition to adulthood. . 展开更多
关键词 HIV ADOLESCENT ACCEPTANCE experience
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Qualitative exploration of home life experiences and care needs among elderly patients with temporary intestinal stomas
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作者 Si-Meng Wang Jian-Ling Jiang +5 位作者 Rui Li Juan-Juan Wang Chun-Hong Gu Jia Zeng Xiao-Hui Wei Mei Chen 《World Journal of Gastroenterology》 SCIE CAS 2024年第22期2893-2901,共9页
BACKGROUND This study employed a phenomenological research approach within qualitative research to explore the challenges encountered by elderly individuals with temporary colostomies in managing their daily lives and... BACKGROUND This study employed a phenomenological research approach within qualitative research to explore the challenges encountered by elderly individuals with temporary colostomies in managing their daily lives and care needs.Protecting the anus surgery combined with temporary colostomy has emerged as a prevalent treatment modality for low rectal cancer.However,the ileostomy is susceptible to peri-stoma skin complications,as well as fluid,electrolyte,and nutritional imbalances,posing challenges to effective management.The successful selfmanagement of patients is intricately linked to their adjustment to temporary colostomy;nonetheless,there remains a dearth of research examining the factors influencing self-care among temporary colostomy patients and the obstacles they confront.AIM To investigate the lived experiences,perceptions,and care requirements of temporary colostomy patients within their home environment,with the ultimate goal of formulating a standardized management protocol.METHODS Over the period of June to August 2023,a purposive sampling technique was utilized to select 12 patients with temporary intestinal stomas from a tertiary hospital in Shanghai,China.Employing a phenomenological research approach,a semi-structured interview guide was developed,and qualitative interviews were conducted using in-depth interview techniques.The acquired data underwent coding,analysis,organization,and summarization following Colaizzi’s seven-step method.RESULTS The findings of this study revealed that the experiences and needs of patients with temporary intestinal stomas can be delineated into four principal themes:Firstly,Temporary colostomy patients bear various burdens and concerns about the uncertainty of disease progression;secondly,patients exhibit limited self-care capabilities and face information deficits,resulting in heightened reliance on healthcare professionals;thirdly,patients demonstrate the potential for internal motivation through proactive self-adjustment;and finally,patients express a significant need for emotional and social support.CONCLUSION Home-living patients with temporary intestinal stomas confront multifaceted challenges encompassing burdens,inadequate self-care abilities,informational deficits,and emotional needs.Identifying factors influencing patients’self-care at home and proposing strategies to mitigate barriers can serve as a foundational framework for developing and implementing nursing interventions tailored to the needs of patients with temporary intestinal stomas. 展开更多
关键词 ELDERLY Temporary intestinal stomas Life experiences Intestinal ostomy complications Qualitative study
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Experiences of Mothers on the Utilization of Elimination of Mother to Child Transmission of HIV Services at Mtendere Clinic, Lusaka
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作者 Ireen Kasongo Silavwe Patricia Katowa Mukwato +2 位作者 Sebean Mayimbo Febiano Phiri Laura Lubinda 《Journal of Biosciences and Medicines》 2024年第4期298-318,共21页
Introduction: Acquired immunodeficiency syndrome is one of the leading causes of mortality among women of reproductive age and Mother to Child Transmission of Human immunodeficiency virus is still a challenge affectin... Introduction: Acquired immunodeficiency syndrome is one of the leading causes of mortality among women of reproductive age and Mother to Child Transmission of Human immunodeficiency virus is still a challenge affecting many countries. Globally, an estimation of 180,000 children under 15 years acquire the Human immunodeficiency virus every day, and more than 90% of those infections are due to Mother to Child Transmission. The study sought to explore the experiences of mothers on the Elimination of Mother to Child Transmission-HIV services at Mtendere Clinic, Lusaka. Materials and Methods: Qualitative interpretive phenomenology study design was employed using in-depth interviews to collect data from a sample that was selected using purposive sampling technique. Thirteen participants were recruited, and these were HIV-positive mothers at least between the ages of 15 and 49 years and enrolled in the Elimination of Mother To Child Transmission-HIV program. The in-depth interviews were audiotape recorded and transcribed verbatim. Data was analyzed using thematic method. Findings: Three main themes that emerged are;living with HIV, support system and barriers to utilization of Elimination of Mother To Child Transmission-HIV services. Most of the participants expressed having acquired knowledge from the program, and received counselling and support from spouses, family as well as health personnel at Mtendere health facility which culminated into a positive experience and enhanced their uptake of the Elimination of Mother to Child Transmission-HIV services. However, barriers to service utilization were identified and these included fear of stigma, negative attitudes from health workers, long waiting times, lack of support and lack of transport to the health facility. Conclusion: Interventions such as community awareness campaigns on Elimination of Mother To Child Transmission-HIV, male involvement and implementing mother-to-mother peer support strategies in Elimination of Mother To Child Transmission-HIV service utilization should be prioritized so as to alleviate stigma and enhancing a positive experience for these mothers thus reducing on the Mother to Child HIV Transmission burden and mortality rates. 展开更多
关键词 Elimination of Mother-to-Child Transmission experiences Barriers UTILIZATION HIV/AIDS
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Community Caregivers’ Experiences and Programmatic Strategies to Improve Active Case Finding in Community Tuberculosis Care in Botswana 2016-2021
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作者 Unami Modongo-Mathebula Mary Moleki Tefera Agizew 《Journal of Tuberculosis Research》 2024年第2期105-118,共14页
Background: The community tuberculosis care program was started to reduce the impact of TB and increase successful treatment outcomes, thus contributing to meeting WHO targets on TB. According to the Botswana Ministry... Background: The community tuberculosis care program was started to reduce the impact of TB and increase successful treatment outcomes, thus contributing to meeting WHO targets on TB. According to the Botswana Ministry of Health, community tuberculosis care was introduced with the main goal of reducing tuberculosis-related morbidity and mortality among communities through the expansion of direct observed therapy and community involvement to community settings. The community caregivers were to support tuberculosis patients throughout their treatment period until they were cured or had completed their treatment. Settings: Two major cities of Botswana, Francistown and Gaborone, with more than twenty-two health clinics offering tuberculosis care. Objective: To investigate community caregivers’ experiences and identify programmatic strategies to improve active TB case findings under the community TB care (CTBC) program in Botswana during 2016-2021. Methods: We adopted a descriptive qualitative research design, followed by convenience purposive sampling. We obtained consent and interviewed 40 participants who met the inclusion criteria, 16 out of 73 in Gaborone and 24 out of 38 from Francistown. Results: We interviewed 40 caregivers with a mean age of 43.4 years. Accepting the caregiving role was identified as the main experience by more than two thirds of the caregivers. While at least more than two-fifth felt obligated to care for the patient at home due to personal relations. More than half of the respondents found caregiving difficult or frustrating due to some challenges encountered during the caregiving role. Behavioral modifications were suggested under different subthemes as strategies to improve active case finding. [-rId13-]Conclusion: Acceptance was the major experience in CTBC. Intensification of known programmatic strategies was suggested by caregivers to improve tuberculosis active case finding in CTBC. 展开更多
关键词 Community experiences CAREGIVERS Active Case Finding TUBERCULOSIS Community Tuberculosis Care
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Psychological Experience of Mothers of Children with Sickle Cell Disease Followed at the Pediatric Department of Bouaké University Teaching Hospital
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作者 Akanji Iburaima Alamun Aka-Tanoh Koko Aude Hélène +5 位作者 Yao Kouassi Christian Adou Leioh Romeo Sahi Gnantin Josette Landryse Amani Ehi Alexise Eleonore Boune Aboulaye Asse Kouadio Vincent 《Open Journal of Pediatrics》 2024年第1期149-163,共15页
Introduction: Sickle cell disease has physical and emotional repercussions on the child and his family. The aim of this study was to describe the psychosocial experiences of mothers of children with sickle cell diseas... Introduction: Sickle cell disease has physical and emotional repercussions on the child and his family. The aim of this study was to describe the psychosocial experiences of mothers of children with sickle cell disease in order to improve the overall care of the child. Methods: This was a descriptive cross-sectional study carried out in the pediatrics department of Bouaké University Teaching Hospital from June to September 2023. It focused on mothers of major sickle-cell-affected children followed up in the pediatrics department of the Bouaké University Teaching Hospital. The variables studied were sociodemographic, psychological, social and economic. Results: Of the 40 mothers surveyed, 15% were not in school and 32.5% were unemployed. For them, sickle cell disease was of natural (genetic) origin in 90% and supernatural in 10%. They stated that the child had an average age of 36 months (extremes 7 and 108 months) when the disease was discovered. And 52% of them were satisfied with the way the disease was clearly and completely announced. Following the announcement, the questioned mothers said they had felt shock (35%), sadness (31.7%), guilt (23.3%) and discouragement (10%). Anxiety and depression were experienced by 77.5% and 22.5% respectively. In 60% of cases, they stated that the disease was incurable, and the outcome was fatal in 2.5% of cases. The child’s illness was a source of problems in the home in 25% of cases, represented by arguments in 92% and divorce in 8%. In 97.5% of cases, the mother told her family and friends about the child’s illness. In 90% of cases, the mother and child benefited from psychological support from family and friends. Conclusion: Sickle cell disease is a serious illness with a psychological and social impact on mothers. We recommend psychological support for mothers from the moment of diagnosis and throughout follow-up. 展开更多
关键词 Psychosocial experience Sickle Cell Disease Côte d’Ivoire
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Citizens’Mental Health Issues and Psychological Trauma Experience due to a Crowd-Crush Disaster in Korea
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作者 Yun-Jung Choi Jae-Won Kwak Hae-In Namgung 《International Journal of Mental Health Promotion》 2024年第6期439-447,共9页
This study evaluated the state of anxiety, depression, post-traumatic stress disorder, general mental health, and mental well-beingamong citizens after a crowd-crush disaster in Korea. Individuals who experienced the ... This study evaluated the state of anxiety, depression, post-traumatic stress disorder, general mental health, and mental well-beingamong citizens after a crowd-crush disaster in Korea. Individuals who experienced the crowd crush had significantly higheranxiety, depression, and post-traumatic stress disorder (PTSD) scores than those who did not (p < 0.001). Additionally,people who avoided the disaster area had significantly higher depression and PTSD scores than those who did not avoid thearea (p < 0.001). Those who directly witnessed the Seoul Halloween crowd crush had a significant difference in PTSD levels ineither group than those who experienced it indirectly (p = 0.005). There was a significant difference in PTSD scores in cases ofdirect damage or death of an acquaintance (p < 0.001). The Seoul Halloween crowd crush caused psychological damagethrough indiscriminate exposure to the public, and symptoms of PTSD appeared over a long period. It is crucial to provideessential resources for ongoing treatment and case management. 展开更多
关键词 ANXIETY avoidance depression disaster experience mental health PTSD psychological trauma
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UAV maneuvering decision-making algorithm based on deep reinforcement learning under the guidance of expert experience
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作者 ZHAN Guang ZHANG Kun +1 位作者 LI Ke PIAO Haiyin 《Journal of Systems Engineering and Electronics》 SCIE CSCD 2024年第3期644-665,共22页
Autonomous umanned aerial vehicle(UAV) manipulation is necessary for the defense department to execute tactical missions given by commanders in the future unmanned battlefield. A large amount of research has been devo... Autonomous umanned aerial vehicle(UAV) manipulation is necessary for the defense department to execute tactical missions given by commanders in the future unmanned battlefield. A large amount of research has been devoted to improving the autonomous decision-making ability of UAV in an interactive environment, where finding the optimal maneuvering decisionmaking policy became one of the key issues for enabling the intelligence of UAV. In this paper, we propose a maneuvering decision-making algorithm for autonomous air-delivery based on deep reinforcement learning under the guidance of expert experience. Specifically, we refine the guidance towards area and guidance towards specific point tasks for the air-delivery process based on the traditional air-to-surface fire control methods.Moreover, we construct the UAV maneuvering decision-making model based on Markov decision processes(MDPs). Specifically, we present a reward shaping method for the guidance towards area and guidance towards specific point tasks using potential-based function and expert-guided advice. The proposed algorithm could accelerate the convergence of the maneuvering decision-making policy and increase the stability of the policy in terms of the output during the later stage of training process. The effectiveness of the proposed maneuvering decision-making policy is illustrated by the curves of training parameters and extensive experimental results for testing the trained policy. 展开更多
关键词 unmanned aerial vehicle(UAV) maneuvering decision-making autonomous air-delivery deep reinforcement learning reward shaping expert experience
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Experience on the Management of Priapism at the Yaounde Central Hospital
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作者 Philip Fernandez Owon’Abessolo Jean Cédric Fouda +5 位作者 Armel Essomba Cécile Etobe Achille Kpanou Yellem Yvon Gerard Beling Abanda Bright Awondo-Che Milama Steves Ndang Ngou 《Open Journal of Urology》 2024年第4期244-251,共8页
Introduction: Priapism is a urological emergency defined as a prolonged penile erection for more than 6 hours, painful and sustained, occurring without any sexual stimulation. The diagnosis is essentially clinical. Ho... Introduction: Priapism is a urological emergency defined as a prolonged penile erection for more than 6 hours, painful and sustained, occurring without any sexual stimulation. The diagnosis is essentially clinical. However, the management is often late because of the delay in consultation, which is characteristic in our context. The purpose of our study is to describe the clinical and therapeutic aspects of patients who presented with priapism at the Yaoundé Central Hospital as well as to propose a present synthesis concerning the management of priapism. Methodology: We conducted a descriptive cross-sectional study with a retrospective recruitment method in the urology department of the YCH. It extended over a period of 6 months from January 2022 to June 2022. We reviewed the files of patients seen within a period spanning 10 years from January 1<sup>st</sup>, 2011 to January 31<sup>st</sup>, December 2021. The study population consisted of patients who had been hospitalized and treated for priapism. We carried out consecutive and non-exhaustive sampling. Results: During the study, we collected data from the medical files of 13 patients. The median age was 25 years, with extremes of 12 and 82 years. Most of the patients were between 20 and 30 years old (38.5%). Most of the patients were single (69.2%). Sickle cell disease was the main comorbidity encountered during the study (76.9%). The median time from onset of symptoms to consultation was 10 [6 - 20.5] hours, with extremes of 1 and 264 hours. All patients presented with painful penile erection (100%). There was concomitant multifocal pain in 38.5% of cases suggestive of vaso-occlusive crises (VOC). The symptoms occurred in a non-traumatic context in the majority of cases (92.3%). The T-shunt was the most used surgical technique (84.6%). The median duration of surgery was 36 [35 - 40] minutes with extremes of 25 and 60 minutes, the majority being operated in less than 45 minutes (84.6%). The frequency of postoperative complications was 46.2%, dominated by weak erections/loss of erections (38.5%). The median duration of hospitalisation was 4 [3 - 5.5] days, with extremes 2 and 20 days of hospitalisation. Resumption of sexual activity was reported in 8 patients (61.5%). The time to recovery was mostly greater than 6 weeks (62.5%). Conclusion: Priapism remains a urological emergency. The prognosis depends on the patients’ promptness in consulting. The T-Shunt is an effective surgical technique in the event of failure of medical treatment. 展开更多
关键词 PRIAPISM TREATMENT experience
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First experience in laparoscopic surgery in low and middle income countries: A systematic review
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作者 Rebekka Troller Jasmine Bawa +1 位作者 Olivia Baker James Ashcroft 《World Journal of Gastrointestinal Surgery》 SCIE 2024年第2期546-553,共8页
BACKGROUND Laparoscopic surgery has reduced morbidity and mortality rates,shorter post-operative recovery periods and lower complication rates than open surgery.It is routine practice in high-income countries and is b... BACKGROUND Laparoscopic surgery has reduced morbidity and mortality rates,shorter post-operative recovery periods and lower complication rates than open surgery.It is routine practice in high-income countries and is becoming increasingly common in countries with limited resources.However,introducing laparoscopic surgery in low-and-middle-income countries(LMIC)can be expensive and requires resour-ces,equipment,and trainers.AIM To report the challenges and benefits of introducing laparoscopic surgery in LMIC as well as to identify solutions to these challenges for countries with limited finances and resources.METHODS MEDLINE,EMBASE and Cochrane databases were searched for studies reporting first experience in laparoscopic surgery in LMIC.Included studies were published between 1996 and 2022 with full text available in English.Exclusion criteria were studies considering only open surgery,ear,nose,and throat,endoscopy,arthro-scopy,hysteroscopy,cystoscopy,transplant,or bariatric surgery.RESULTS Ten studies out of 3409 screened papers,from eight LMIC were eligible for inclusion in the final analysis,totaling 2497 patients.Most reported challenges were related to costs of equipment and training programmes,equipment pro-blems such as faulty equipment,and access to surgical kits.Training-related challenges were reliance on foreign trainers and lack of locally trained surgeons and theatre staff.The benefits of introducing laparoscopic surgery were economic and clinical,including a reduction in hospital stay,complications,and morbidi-ty/mortality.The introduction of laparoscopic surgery also provided training opportunities for junior doctors.CONCLUSION Despite financial and technical challenges,many studies emphasise the overall benefit of introducing laparoscopic surgery in LMICs such as reduced hospital stay and the related lower cost for patients.While many of the clinical centres in LMICs have proposed practical solutions to the challenges reported,more support is critically required,in particular regarding training. 展开更多
关键词 Laparoscopic surgery Low and middle income country First experience Training in laparoscopic surgery
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