To investigate the radioimmunotherapeutic efficacy, radio-immunoconjugate 131-I-3G9, 811-I-3H11 and 131-I-NMIgG (irre levant antibody) were i.p. injected into nude mice bearing human gastric cancer xenograftes. Each a...To investigate the radioimmunotherapeutic efficacy, radio-immunoconjugate 131-I-3G9, 811-I-3H11 and 131-I-NMIgG (irre levant antibody) were i.p. injected into nude mice bearing human gastric cancer xenograftes. Each animal received a single doses of 555MBq. Over 14 days the accumulative absorbed doses in tumors were 13.7 Gy for 131-I-3H11 and 12.17 Gy for 131-I-3G9. Both were significantly higher than that for 131-I-NMIgG (3.23 Gy). Thera peutic efficacy appeared most sharply from 2 to 3 weeks after injection. The inhibition ratio of tumor were 86% and 70% for 131-I-3H11 and 131-I-3G9 respectively. Histopathological evidance indicated that in tumor tissues radioactive damage was showed as karyopyknosis, karyorrhexis and necrosis or partial disappearance of tumor cells, while in the other tissues no radioactive damage was observed. WBC counts of all animals did not show significant difference before and after treatment, which indicated that the haemopoietic function of bone marrow was not affected.展开更多
Objective: To study the treatment of experimental metastatic lung carcinoma by intratracheal injection of IL-l8 gene recombinant adenovirus. Methods: (1)The mouse IL-18 mRNA was detected by RT-PCR, and the concentrati...Objective: To study the treatment of experimental metastatic lung carcinoma by intratracheal injection of IL-l8 gene recombinant adenovirus. Methods: (1)The mouse IL-18 mRNA was detected by RT-PCR, and the concentration of IL-18 and associated cytokines in lung lavages and blood were determined by ELISA at different time points after intratracheal injection of IL-18 recombinant adenovirus. (2)The lung metastasis nodes, mouse survival periods and survival rates were evaluated. NK activity and CTL activity were determined by 51Cr 4 h release method. Results: (1) IL-18 mRNA was detectable in lung tissue 6 h after intratracheal use of IL-18 recombinant adenovirus. and the concentration of IL-18 in lung lavage was higher than that in peripheral blood. Neither IL-18 mRNA nor IL-18 was detectable in control group. (2) Intratracheal use of IL-18 recombinant adenovirus resulted in increased CTL and NK activity, longer survival time and higher survival rates compared with the control group, showing significant therapeutic effect on expermental lung metastasis. Conclusion: Intratracheal use of adenovirus vector containing IL- 18 gene has therapeutic effect on the lung metastasis, denoting that gene therapy of lung diseases could be applied through airway directly with recombinant adenovirus.展开更多
Objectives: To explore the effect of acupuncture on cerebral infarction in rats and to try providing some experimental parameters for clinical practice. Methods: 27 healthy Wistar rats were randomly divided into pseud...Objectives: To explore the effect of acupuncture on cerebral infarction in rats and to try providing some experimental parameters for clinical practice. Methods: 27 healthy Wistar rats were randomly divided into pseudo operation (n=10), model (n=8) and acupuncture (n=9) groups. Neuro functional defect scoring, apoptosis of single brain slice and the number of bcl 2 immuno reaction (IR) positive neurons in CA1 area of the hippocampus were used as the indexes to investigate the possible mechanisms of acupuncture of "Nie San Zhen" (needling three acupoints in the temporal region) and "Si Shen Zhen" (needling four acupoints in the occiput region) in treating rats with cerebral infarction. Results: There existed significant differences between acupuncture group and model group in improving neurologic functional activities, inhibiting apoptosis of the brain cells and increasing bcl 2 IR positive neurons in the hippocampal CA1 area ( P <0.01). Conclusion: Acupuncture therapy can improve cerebral infarction in the rat by suppressing apoptosis and up regulation of the bcl 2 IR positive neuron expression.展开更多
To investigate the therapeutic effect of nigh-dosage γ-aminobutyric acid （GABA） on acute tetramine （TET） poisoning, 50 Kunming mice were divided into 5 groups at random and the antidotal effects of GABA or sodium...To investigate the therapeutic effect of nigh-dosage γ-aminobutyric acid （GABA） on acute tetramine （TET） poisoning, 50 Kunming mice were divided into 5 groups at random and the antidotal effects of GABA or sodium dimercaptopropane sulfonate （Na-DMPS） on poisoned mice in different groups were observed in order to compare the therapeutic effects of nigh-dosage GABA with those of Na-DMPS. Slices of brain tissue of the poisoned mice were made to examine pathological changes of cells. The survival analysis was employed. Our results showed that both high-dosage GABA and Na-DMPS could obviously prolong the survival time, delay onset of convulsion and muscular twitch, and ameliorate the symptoms after acute tetramine poisoning in the mice. Better effects could be achieved with earlier use of high dosage GABA or Na-DMPS. There was no significant difference in prolonging the survival time between high-dose GABA and Na-DMPS used immediately after poisioning. It is concluded that high-dosage GABA can effectively antagonize acute toxicity of terarnine in mice. And it is suggested that nigh-dosage GABA may be used as an excellent antidote for acute TET poisoning in clinical practice. The indications and correct dosage for clinical use awaits to be further studied.展开更多
MIT method was applied to assay the cytotoxicityof three reversors, verapamil (VER), dipyridamole (DPM)and cyclosporin (CSA) in K562, K562/ADM and KB celllines. S-P immunocytochemical technique was applied todetect cx...MIT method was applied to assay the cytotoxicityof three reversors, verapamil (VER), dipyridamole (DPM)and cyclosporin (CSA) in K562, K562/ADM and KB celllines. S-P immunocytochemical technique was applied todetect cxpressions of oncoproteins or tumor suppressoroncoproteins in these tumor cell lines before or aftertreatment with these reversors. Results showed that threereversors were capable of inhibiting to a certain extentgrowth of K562 or KB cell lines and reversing greatlyadriamycin (ADM)-resistance in K562/ADM cell line.DPM and CsA were observed to inhibit, partly or wholly,expressions of p53, p16, bcl-2, p21 or cerbB-2oncoproteins. VER showed whole inhibition ofexpressions of P53, p16, p21 and bcl-2 and partlyexpression of p53 oncoprotein in K562 cell line. Theseresults suggest that growth inhibition in K562 or KB celllines by the reversors may be associated with partial orwhole inhibition or expressions of p53, p16, p21 or c-erbB-2 oncoproteins. Inhibitions of expressions p53, p16,p21 oncoproteins by VER but not DPM or CsA, may berespossible for reversing activity of VER for ADM-resistance in K562/ADM cell line.展开更多
Objective: To determine the effect of ascorbic acid (AA) on DNA synthesis, intracellular accumulation of ADM and ADM resistance of tumor cell lines. Methods: K562, K562/ADM and KB cell lines were used to study the e...Objective: To determine the effect of ascorbic acid (AA) on DNA synthesis, intracellular accumulation of ADM and ADM resistance of tumor cell lines. Methods: K562, K562/ADM and KB cell lines were used to study the effect of ascorbic acid on DNA synthesis, intracellular accumulation of ADM and ADM resistance by fluid scintillometry, MTT method, spectrofluorophotometry and immunocytochemistry. Results: Results showed that AA was capable of inhibiting DNA synthesis of K562 and K562/ADM in a dosedependence fashion, but not KB cell line, and significantly reducing ADM sensitivity in K562 and KB cell lines, as well as potentiating obviously ADM resistance in K562/ADM cell line. Conclusion: These effects of AA may be closely correlated with significant elevation of intracellular accumulation of ADM in KB cell line, and significant reduction of that in K562 and K562/ADM cell lines but possibly not correlated with the expression of Pglycoprotein.展开更多
Objective: To understand whether verapamil (VER) resistance development in the multidrug-resistant cell line and its mechanism. Methods: K562/ADM/VER cell subline resistant to verapamil was established through a gradu...Objective: To understand whether verapamil (VER) resistance development in the multidrug-resistant cell line and its mechanism. Methods: K562/ADM/VER cell subline resistant to verapamil was established through a gradual increase of VER concentration in the media. MTT method was used to assay resistance to VER, cross resistance to dipyriamole (DPM), cyclosporin A (CsA) in the cells, and HPLC and spectrofluorometer to detect intracellular accumulation of VER or ADM respectively, as well as S-P immunocytochemical technique for detection of genes expression. Results: It were observed that 7.9—fold increase in VER resistance, significantly reduced intracellular accumulation of VER or ADM and also develop across resistance to DPM and CsA in K562/ADM/VER cells, compared with its parent cell, K562/ADM. High-level of p-glycoprotein(pgp), middle-level of p53, p16, was present in two cell lines without expression of GSTPI, C-myc, C-myc, C-fos and C-erbB-2. Bc1–2 protein expression was found only in K562/ADM cells. Conclusion: K562/ADM cells were capable of being induced to develop resistance to VER.展开更多
In the present study to investigate the biochemical mechanisms of therapeutic and prophylactic action of bioflavonoids, carried out a comparative evaluation of antioxidant and antiproteinase properties of certain biof...In the present study to investigate the biochemical mechanisms of therapeutic and prophylactic action of bioflavonoids, carried out a comparative evaluation of antioxidant and antiproteinase properties of certain bioflavonoids standards in vitro. Therapeutic and prophylactic efficacy of individual bioflavonoids as well as herbal medicines with bioflavonoids, was examined at an experimental pathology （toxic hepatitis, dental caries, periodontitis, stomatitis, dysbiosis, diabetes Types 1 and 2, gastric ulcer, osteopenia） in Wistar line rats. Condition of organs and tissues was assessed by biochemical markers of inflammation, antioxidant and antimicrobial defense systems of animals. Research has shown the ability ofbioflavonoids in varying degrees inhibit the formation of superoxide anion radicals and malondialdehyde, recover free radicals, bind ions of Fe2＋, inhibiting the activity of proteases, such as leukocyte elastase. Established partially competitive type of trypsin and elastase activity inhibition by bioflavonoids. Was revealed a positive effect of bioflavonoids in experimental pathology on animals. Therapeutic and prophylactic effects of bioflavonoids, in our opinion, are realized through a strong antioxidant and antiprotease properties of these compounds.展开更多
Glioblastoma(GBM)is one of the most aggressive(grade IV)gliomas characterized by a high rate of recurrence,resistance to therapy and a grim survival prognosis.The long-awaited improvement in GBM patients'survival ...Glioblastoma(GBM)is one of the most aggressive(grade IV)gliomas characterized by a high rate of recurrence,resistance to therapy and a grim survival prognosis.The long-awaited improvement in GBM patients'survival rates essentially depends on advances in the development of new therapeutic approaches.Recent preclinical studies show that nanoscale materials could greatly contribute to the improvement of diagnosis and management of brain cancers.In the current review,we will discuss how specific features of glioma pathobiology can be employed for designing efficient targeting approaches.Moreover,we willsummarize the main evidence for the potential of the IL-13R alpha 2 receptor(IL13α2R)targeting in GBM early diagnosis and experimental therapy.展开更多
文摘To investigate the radioimmunotherapeutic efficacy, radio-immunoconjugate 131-I-3G9, 811-I-3H11 and 131-I-NMIgG (irre levant antibody) were i.p. injected into nude mice bearing human gastric cancer xenograftes. Each animal received a single doses of 555MBq. Over 14 days the accumulative absorbed doses in tumors were 13.7 Gy for 131-I-3H11 and 12.17 Gy for 131-I-3G9. Both were significantly higher than that for 131-I-NMIgG (3.23 Gy). Thera peutic efficacy appeared most sharply from 2 to 3 weeks after injection. The inhibition ratio of tumor were 86% and 70% for 131-I-3H11 and 131-I-3G9 respectively. Histopathological evidance indicated that in tumor tissues radioactive damage was showed as karyopyknosis, karyorrhexis and necrosis or partial disappearance of tumor cells, while in the other tissues no radioactive damage was observed. WBC counts of all animals did not show significant difference before and after treatment, which indicated that the haemopoietic function of bone marrow was not affected.
基金National Natural Science Foundation of China (No.39730420 )
文摘Objective: To study the treatment of experimental metastatic lung carcinoma by intratracheal injection of IL-l8 gene recombinant adenovirus. Methods: (1)The mouse IL-18 mRNA was detected by RT-PCR, and the concentration of IL-18 and associated cytokines in lung lavages and blood were determined by ELISA at different time points after intratracheal injection of IL-18 recombinant adenovirus. (2)The lung metastasis nodes, mouse survival periods and survival rates were evaluated. NK activity and CTL activity were determined by 51Cr 4 h release method. Results: (1) IL-18 mRNA was detectable in lung tissue 6 h after intratracheal use of IL-18 recombinant adenovirus. and the concentration of IL-18 in lung lavage was higher than that in peripheral blood. Neither IL-18 mRNA nor IL-18 was detectable in control group. (2) Intratracheal use of IL-18 recombinant adenovirus resulted in increased CTL and NK activity, longer survival time and higher survival rates compared with the control group, showing significant therapeutic effect on expermental lung metastasis. Conclusion: Intratracheal use of adenovirus vector containing IL- 18 gene has therapeutic effect on the lung metastasis, denoting that gene therapy of lung diseases could be applied through airway directly with recombinant adenovirus.
文摘Objectives: To explore the effect of acupuncture on cerebral infarction in rats and to try providing some experimental parameters for clinical practice. Methods: 27 healthy Wistar rats were randomly divided into pseudo operation (n=10), model (n=8) and acupuncture (n=9) groups. Neuro functional defect scoring, apoptosis of single brain slice and the number of bcl 2 immuno reaction (IR) positive neurons in CA1 area of the hippocampus were used as the indexes to investigate the possible mechanisms of acupuncture of "Nie San Zhen" (needling three acupoints in the temporal region) and "Si Shen Zhen" (needling four acupoints in the occiput region) in treating rats with cerebral infarction. Results: There existed significant differences between acupuncture group and model group in improving neurologic functional activities, inhibiting apoptosis of the brain cells and increasing bcl 2 IR positive neurons in the hippocampal CA1 area ( P <0.01). Conclusion: Acupuncture therapy can improve cerebral infarction in the rat by suppressing apoptosis and up regulation of the bcl 2 IR positive neuron expression.
基金This project was supported by a grant from Research and Development Program of Science and Technology of Hubei Province (No 2002AA301C93)
文摘To investigate the therapeutic effect of nigh-dosage γ-aminobutyric acid （GABA） on acute tetramine （TET） poisoning, 50 Kunming mice were divided into 5 groups at random and the antidotal effects of GABA or sodium dimercaptopropane sulfonate （Na-DMPS） on poisoned mice in different groups were observed in order to compare the therapeutic effects of nigh-dosage GABA with those of Na-DMPS. Slices of brain tissue of the poisoned mice were made to examine pathological changes of cells. The survival analysis was employed. Our results showed that both high-dosage GABA and Na-DMPS could obviously prolong the survival time, delay onset of convulsion and muscular twitch, and ameliorate the symptoms after acute tetramine poisoning in the mice. Better effects could be achieved with earlier use of high dosage GABA or Na-DMPS. There was no significant difference in prolonging the survival time between high-dose GABA and Na-DMPS used immediately after poisioning. It is concluded that high-dosage GABA can effectively antagonize acute toxicity of terarnine in mice. And it is suggested that nigh-dosage GABA may be used as an excellent antidote for acute TET poisoning in clinical practice. The indications and correct dosage for clinical use awaits to be further studied.
文摘MIT method was applied to assay the cytotoxicityof three reversors, verapamil (VER), dipyridamole (DPM)and cyclosporin (CSA) in K562, K562/ADM and KB celllines. S-P immunocytochemical technique was applied todetect cxpressions of oncoproteins or tumor suppressoroncoproteins in these tumor cell lines before or aftertreatment with these reversors. Results showed that threereversors were capable of inhibiting to a certain extentgrowth of K562 or KB cell lines and reversing greatlyadriamycin (ADM)-resistance in K562/ADM cell line.DPM and CsA were observed to inhibit, partly or wholly,expressions of p53, p16, bcl-2, p21 or cerbB-2oncoproteins. VER showed whole inhibition ofexpressions of P53, p16, p21 and bcl-2 and partlyexpression of p53 oncoprotein in K562 cell line. Theseresults suggest that growth inhibition in K562 or KB celllines by the reversors may be associated with partial orwhole inhibition or expressions of p53, p16, p21 or c-erbB-2 oncoproteins. Inhibitions of expressions p53, p16,p21 oncoproteins by VER but not DPM or CsA, may berespossible for reversing activity of VER for ADM-resistance in K562/ADM cell line.
文摘Objective: To determine the effect of ascorbic acid (AA) on DNA synthesis, intracellular accumulation of ADM and ADM resistance of tumor cell lines. Methods: K562, K562/ADM and KB cell lines were used to study the effect of ascorbic acid on DNA synthesis, intracellular accumulation of ADM and ADM resistance by fluid scintillometry, MTT method, spectrofluorophotometry and immunocytochemistry. Results: Results showed that AA was capable of inhibiting DNA synthesis of K562 and K562/ADM in a dosedependence fashion, but not KB cell line, and significantly reducing ADM sensitivity in K562 and KB cell lines, as well as potentiating obviously ADM resistance in K562/ADM cell line. Conclusion: These effects of AA may be closely correlated with significant elevation of intracellular accumulation of ADM in KB cell line, and significant reduction of that in K562 and K562/ADM cell lines but possibly not correlated with the expression of Pglycoprotein.
文摘Objective: To understand whether verapamil (VER) resistance development in the multidrug-resistant cell line and its mechanism. Methods: K562/ADM/VER cell subline resistant to verapamil was established through a gradual increase of VER concentration in the media. MTT method was used to assay resistance to VER, cross resistance to dipyriamole (DPM), cyclosporin A (CsA) in the cells, and HPLC and spectrofluorometer to detect intracellular accumulation of VER or ADM respectively, as well as S-P immunocytochemical technique for detection of genes expression. Results: It were observed that 7.9—fold increase in VER resistance, significantly reduced intracellular accumulation of VER or ADM and also develop across resistance to DPM and CsA in K562/ADM/VER cells, compared with its parent cell, K562/ADM. High-level of p-glycoprotein(pgp), middle-level of p53, p16, was present in two cell lines without expression of GSTPI, C-myc, C-myc, C-fos and C-erbB-2. Bc1–2 protein expression was found only in K562/ADM cells. Conclusion: K562/ADM cells were capable of being induced to develop resistance to VER.
文摘In the present study to investigate the biochemical mechanisms of therapeutic and prophylactic action of bioflavonoids, carried out a comparative evaluation of antioxidant and antiproteinase properties of certain bioflavonoids standards in vitro. Therapeutic and prophylactic efficacy of individual bioflavonoids as well as herbal medicines with bioflavonoids, was examined at an experimental pathology （toxic hepatitis, dental caries, periodontitis, stomatitis, dysbiosis, diabetes Types 1 and 2, gastric ulcer, osteopenia） in Wistar line rats. Condition of organs and tissues was assessed by biochemical markers of inflammation, antioxidant and antimicrobial defense systems of animals. Research has shown the ability ofbioflavonoids in varying degrees inhibit the formation of superoxide anion radicals and malondialdehyde, recover free radicals, bind ions of Fe2＋, inhibiting the activity of proteases, such as leukocyte elastase. Established partially competitive type of trypsin and elastase activity inhibition by bioflavonoids. Was revealed a positive effect of bioflavonoids in experimental pathology on animals. Therapeutic and prophylactic effects of bioflavonoids, in our opinion, are realized through a strong antioxidant and antiprotease properties of these compounds.
基金This work was financed by the Ministry of Science and Higher Education of the Russian Federation within the framework of state support for the creation and development of World-Class Research Centers"Digital Biodesign and Personalized Healthcare"(No.075-15-2020-926).
文摘Glioblastoma(GBM)is one of the most aggressive(grade IV)gliomas characterized by a high rate of recurrence,resistance to therapy and a grim survival prognosis.The long-awaited improvement in GBM patients'survival rates essentially depends on advances in the development of new therapeutic approaches.Recent preclinical studies show that nanoscale materials could greatly contribute to the improvement of diagnosis and management of brain cancers.In the current review,we will discuss how specific features of glioma pathobiology can be employed for designing efficient targeting approaches.Moreover,we willsummarize the main evidence for the potential of the IL-13R alpha 2 receptor(IL13α2R)targeting in GBM early diagnosis and experimental therapy.
