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Research on Improvement Strategies of Psychological Training and Stage Expressiveness in Percussion Performance
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作者 Yu Yan 《Journal of Contemporary Educational Research》 2024年第10期240-246,共7页
With the continuous development of music education,percussion,as an important form of performance,has led to growing attention to the psychological training of its performers.This study aims to explore how psychologic... With the continuous development of music education,percussion,as an important form of performance,has led to growing attention to the psychological training of its performers.This study aims to explore how psychological factors in percussion performance impact stage expressiveness and to propose corresponding psychological training strategies.By analyzing relevant domestic and international literature,we found that psychological training not only enhances performers’confidence and alleviates performance anxiety but also contributes to an overall improvement in performance quality.This study shows that methods such as emotional management and cognitive restructuring exhibit promising application potential in practice.Therefore,exploring a systematic psychological training program is significant for improving the stage expressiveness of percussion performers. 展开更多
关键词 Percussion performance techniques Psychological training Stage expressiveness Emotional management Cognitive restructuring
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The Impact of Contextual Teaching Method on Sichuan Folk Song Education and Students’Musical Expressiveness
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作者 Jing Li 《Journal of Contemporary Educational Research》 2024年第9期100-105,共6页
This study explores the application of the contextual teaching method in Sichuan folk song education and its impact on students’musical expressiveness.By incorporating contextual teaching methods in music classes,thi... This study explores the application of the contextual teaching method in Sichuan folk song education and its impact on students’musical expressiveness.By incorporating contextual teaching methods in music classes,this research investigates the effectiveness of this approach in enhancing students’understanding of Sichuan folk songs and improving their musical expressiveness and emotional expression.A mixed-method research approach is employed,utilizing classroom observations,questionnaires,interviews,and statistical analysis to assess the practical outcomes of contextual teaching in folk song education. 展开更多
关键词 Contextual teaching Sichuan folk songs Musical expressiveness Music education
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Efficient Expressive Attribute-Based Encryption with Keyword Search over Prime-Order Groups
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作者 Qing Miao Lan Guo +1 位作者 Yang Lu Zhongqi Wang 《Intelligent Automation & Soft Computing》 SCIE 2023年第3期2737-2754,共18页
Attribute-based encryption with keyword search(ABEKS)is a novel cryptographic paradigm that can be used to implementfine-grained access control and retrieve ciphertexts without disclosing the sensitive information.It i... Attribute-based encryption with keyword search(ABEKS)is a novel cryptographic paradigm that can be used to implementfine-grained access control and retrieve ciphertexts without disclosing the sensitive information.It is a perfect combination of attribute-based encryption(ABE)and public key encryption with keyword search(PEKS).Nevertheless,most of the existing ABEKS schemes have limited search capabilities and only support single or simple conjunctive keyword search.Due to the weak search capability and inaccurate search results,it is difficult to apply these schemes to practical applications.In this paper,an effi-cient expressive ABEKS(EABEKS)scheme supporting unbounded keyword uni-verse over prime-order groups is designed,which supplies the expressive keyword search function supporting the logical connectives of“AND”and“OR”.The proposed scheme not only leads to low computation and communica-tion costs,but also supports unbounded keyword universe.In the standard model,the scheme is proven to be secure under the chosen keyword attack and the cho-sen plaintext attack.The comparison analysis and experimental results show that it has better performance than the existing EABEKS schemes in the storage,com-putation and communication costs. 展开更多
关键词 Searchable encryption expressive keyword search attribute-based encryption unbounded keyword universe prime-order group
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The Possibility of Semantic Features and Analysis of Figures of Speech —— Cognitive Figures and Expressive Figures 被引量:2
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作者 Liu Dawei Wang Zhiguang 《宏观语言学》 2007年第1期10-24,共15页
In this paper, linguistic methodology is suggested for approaching figures of speech formed due to shift of cognitive relations. First, requisite features, possible features and impossible features are proposed on the... In this paper, linguistic methodology is suggested for approaching figures of speech formed due to shift of cognitive relations. First, requisite features, possible features and impossible features are proposed on the basis of semantic analysis and their interrelationships and expressions are discussed. Then it is further argued that all figures formed due to shift of cognitive relation are expressed to accept an impossible feature; and there are four semantic forms to accept it: “zero distance/distanced”, “implicit/explicit”, “direct/indirect”, “positive/negative”. Finally, starting from the two approaches to figures we comment on the traditional idea of separating decoration and substance and the new idea of “function as form”. 展开更多
关键词 FIGURES of SPEECH requisite FEATURES POSSIBLE FEATURES impossible FEATURES COGNITIVE expressive
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Analysis of English Majors' Expressiveness of Chinese Culture in English
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作者 于慧玲 《海外英语》 2012年第7X期107-108,共2页
Currently English has spread around the world so extensively that it has become a link language across many cultures.Since 1980s,China saw a warm discussion of language,culture and cross-cultural communication.An incr... Currently English has spread around the world so extensively that it has become a link language across many cultures.Since 1980s,China saw a warm discussion of language,culture and cross-cultural communication.An increasing number of researchers and educators began to be aware of the vital importance of culture in EFL teaching and learning and cultural content has been available in classrooms teaching.However,the development of English teaching and cross-cultural communicative competence is target culture-oriented and the ability of expressiveness of home culture in English is almost ignored.Thus,the thesis seeks to collect the students' perceptions on expressiveness of Chinese culture in English and the culture teaching in EFL in China. 展开更多
关键词 ENGLISH majors’ expressiveNESS CROSS-CULTURAL COMM
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Versatile Routing and Self-Certifying Features Support for Secure Mobility in eXpressive Internet Architecture
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作者 Hongwei Meng Zhong Chen +2 位作者 Jianbin Hu Chuck Song Cong Tang 《China Communications》 SCIE CSCD 2017年第4期73-88,共16页
Integrating mobility and security in the network layer has become a key factor for Future Internet Architecture(FIA). This paper proposes a secure mobility support mechanism in e Xpressive Internet Architecture(XIA),a... Integrating mobility and security in the network layer has become a key factor for Future Internet Architecture(FIA). This paper proposes a secure mobility support mechanism in e Xpressive Internet Architecture(XIA),a new FIA currently under development as part of the US National Science Foundation's(NSF) program. Utilizing the natural features of ID/locator decoupling and versatile routing in XIA, a general mechanism to support host mobility is proposed. Exploiting the self-certifying identifier, a secure binding update protocol to overcome the potential threats introduced by the proposed mobility support mechanism is also given. We demonstrate that our design in XIA outperforms IP based solutions in terms of efficiency and flexibility. We also outline our initial design to illustrate one derivative benefit of an evolvable architecture:mobility support customizability with no sacrifice of architectural generality. 展开更多
关键词 future Internet architecture expressive Internet architecture MOBILITY security self-certifying
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Posttraumatic stress symptom trajectories of Chinese university students during the first eight months of the COVID-19 pandemic and their association with cognitive reappraisal,expressive suppression,and posttraumatic growth
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作者 Hong-juan Jiang Jiang Nan +1 位作者 Zhi-yue Lv Juan Yang 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2022年第10期451-460,共10页
Objective: To identify the moderating effects of cognitive reappraisal(CR) and expressive suppression(ES) on the relationship between posttraumatic stress(PTS) symptoms and posttraumatic growth(PTG) in university stud... Objective: To identify the moderating effects of cognitive reappraisal(CR) and expressive suppression(ES) on the relationship between posttraumatic stress(PTS) symptoms and posttraumatic growth(PTG) in university students. Methods: The survey included 1 987 Chinese university students who completed questionnaires on PTS symptoms in February 2020, with three follow-up surveys at two-month intervals until August 2020. We assessed CR and ES at February 2020 and PTG at August 2020. Growth mixture modeling was used to classify the PTS symptom trajectories. Multinomial logistic regression was used to recognize the predictors of class membership. The relationships among PTS symptoms, CR, ES, and PTG were examined using multi-group path analysis.Results: Sex, SARS-Co V-2 infection of a family member or friend, number of siblings, CR, and ES were significantly associated with PTS symptoms. Three latent classes were identified: ‘Increasing PTS’(n=205, 10.0%) who had rapid deterioration of PTS symptoms, ‘Moderate PTS’(n=149, 8.0%) who had a high level of PTS symptoms at the beginning and slightly increasing, and ‘Persistent Minimal PTS’(n=1 633, 82.0%), who had slow resolution of PTS symptoms over time. Male, SARS-Co V-2 infection of a family member or friend, and having a lower CR and a higher ES, were more likely to have ‘Increasing PTS’. PTS at February 2020 predicted PTG only in ‘Increasing PTS’ class, and both CR and ES had moderating effects on the conversion between them.Conclusions: Most students recovered from posttraumatic stress of COVID-19 pandemic, but a small proportion expeienced increasing PTS symptoms, and those with this condition may benefit from emotional regulation intervention. 展开更多
关键词 COVID-19 pandemic Posttraumatic stress symptoms Cognitive reappraisal expressive suppression Posttraumatic growth Chinese university students
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Effect of Ginsenoside Rbl on Expressive Proteome of Rat Neurons
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作者 Chunyan ZHANG Qian XU +1 位作者 Chunling ZHAO Chunlai ZHANG 《Agricultural Biotechnology》 CAS 2012年第2期17-19,共3页
[ Objective ] This study aimed to explore the effect of ginsenoside Rbl on expressive proteome of rat neurons by technologies of proteomics, bioinformat- ies and MS peptide fingerprinting. [ Method ] Rat neurons were ... [ Objective ] This study aimed to explore the effect of ginsenoside Rbl on expressive proteome of rat neurons by technologies of proteomics, bioinformat- ies and MS peptide fingerprinting. [ Method ] Rat neurons were cultured conventionally and randomly separated into two groups. The experimental group was trea- ted with 5 μg/rnl Rbl for 20 min, while control group was added with the same amount of medium. After cell lysis, the whole-cell protein was extracted. Two-di- mensional electrophoresis (2-DE) was used to separate the extracts. Differential expression of proteome between the two groups was analyzed by using ImageMaster 2D Platinum v5.0 software and the two protein spots expressed differently were selected for differential identification with MALDI-TOF-MS. [ Result] Based on the matching and comparative analysis of the protein spots, 418 protein spots were detected in experimental group, including 226 protein spots with differently expres- sive levels; according to the mass spectrometry, the two ginsenoside Rbl-related and differentially-expressed protein spots were identified as cytochrome P-450 and phosducin-like protein, and both of them were phosphorylated proteins. [ Conclusion ] This study showed that the functions of those identified proteins were in- volved in signal transduction, suggesting that the effect of ginsenoside Rbl on expressive proteome of rat neurons might be related to the corresponding signal trans- duction networks. 展开更多
关键词 Ginsenoside Rbl Rat neurons expressive proteome BIOINFORMATICS
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Adorno on Art as Expressive Language
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作者 Feng Tao 《Sociology Study》 2014年第5期438-445,共8页
Adorno suggests that art is an essentially expressive language since it is the imitation of natural language. Adorno's language thought is derived from Benjamin's extensive language theory in which Benjamin claims t... Adorno suggests that art is an essentially expressive language since it is the imitation of natural language. Adorno's language thought is derived from Benjamin's extensive language theory in which Benjamin claims that all things including human being have languages. The telos of art's expression is expressing what the repressed things and human long for speaking. The authentic art preserves the trace of primordial human's instinct: mimesis. Through the subject miming the object in the expression of art, the real cognition is realized. According to this model, we can ascertain that artist is the real epistemological subject, the "collective subject" is factually the speaker of the expression and the epistemological objet which is primary in the production of art. Since art is a language, the standard of art is truth-false, and therefore, the truth content is the criterion of authentic art. Negativity is the core character of truth content. 展开更多
关键词 ART expressive language MIMESIS truth content
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Expressive study on realizing safe and quick blood supplement
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《中国输血杂志》 CAS CSCD 2001年第S1期321-,共1页
关键词 expressive study on realizing safe and quick blood supplement
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Control Emotion Intensity for LSTM-Based Expressive Speech Synthesis
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作者 Xiaolian Zhu Liumeng Xue 《国际计算机前沿大会会议论文集》 2019年第2期654-656,共3页
To improve the performance of human-computer interaction interfaces, emotion is considered to be one of the most important factors. The major objective of expressive speech synthesis is to inject various expressions r... To improve the performance of human-computer interaction interfaces, emotion is considered to be one of the most important factors. The major objective of expressive speech synthesis is to inject various expressions reflecting different emotions to the synthesized speech. To effectively model and control the emotion, emotion intensity is introduced for expressive speech synthesis model to generate speech conveyed the delicate and complicate emotional states. The system was composed of an emotion analysis module with the goal of extracting control emotion intensity vector and a speech synthesis module responsible for mapping text characters to speech waveform. The proposed continuous variable “perception vector” is a data-driven approach of controlling the model to synthesize speech with different emotion intensities. Compared with the system using a one-hot vector to control emotion intensity, this model using perception vector is able to learn the high-level emotion information from low-level acoustic features. In terms of the model controllability and flexibility, both the objective and subjective evaluations demonstrate perception vector outperforms one-hot vector. 展开更多
关键词 EMOTION INTENSITY expressive SPEECH synthesis CONTROLLABLE TEXT-TO-SPEECH NEURAL networks
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Application and Practice of Expressive Painting in Middle School Art Education
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作者 Wen Meng Lei Cai 《Journal of Contemporary Educational Research》 2021年第10期104-108,共5页
By combing the theoretical framework of contemporary expressive painting and expressive art therapy,this study explores the application and practice of integrating expressive painting into middle school art education ... By combing the theoretical framework of contemporary expressive painting and expressive art therapy,this study explores the application and practice of integrating expressive painting into middle school art education in hope to provide new ideas for art education and teaching in contemporary middle schools. 展开更多
关键词 Art education expressive painting Art teaching application
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Identifi cation of the differential expressive tumor associated genes in rectal cancers by cDNA microarray 被引量:1
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作者 Xue-Qin Gao Jin-Xiang Han +3 位作者 Zhong-Fa Xu Wei-Dong Zhang Hua-Ning Zhang Hai-Yan Huang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第3期341-348,共8页
AIM: To identify tumor associated genes of rectal cancer and to probe the application possibility of gene expression profiles for the classification of tumors.METHODS: Rectal cancer tissues and their paired normal m... AIM: To identify tumor associated genes of rectal cancer and to probe the application possibility of gene expression profiles for the classification of tumors.METHODS: Rectal cancer tissues and their paired normal mucosa were obtained from patients undergoing surgical resection of rectal cancer. Total RNA was extracted using Trizol reagents. First strand cDNA synthesis was indirectly labeled with aminoallyl-dUTP and coupled with Cy3 or Cy5 dye NHS mono-functional ester. After normalization to total spots, the genes which background subtracted intensity did not exceed 2 SD above the mean blank were excluded. The data were then sorted to obtain genes differentially expressed by ≥ 2 fold up or down in at least 5 of the 21 patients.RESULTS: In the 21 rectal cancer patients, 23 genes were up-regulated in at least 5 samples and 15 genes were down-regulated in at least 5 patients. Hierachical cluster analysis classified the patients into two groups according to the clinicopathological stage, with one group being all above stage Ⅱ and one group all below stage Ⅱ.CONCLUSION: The up-regulated genes and downregulated genes may be molecular markers of rectal cancer. The expression profiles can be used for classification of rectal cancer. 展开更多
关键词 Rectal cancer Tumor associated genes cDNA microarray Differential expression genes
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明清木结构古建筑底层数据标准化研究
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作者 王茹 张小峰 +2 位作者 胡又文 吕明倩 王垚 《湖南大学学报(自然科学版)》 EI CAS CSCD 北大核心 2024年第9期206-216,共11页
现有IFC标准体系中,存在无法表达中国古建筑中特有的形制、结构、属性、艺术等特性的问题.基于UML,构建了斗栱、榫卯等古建筑结构的语义关系图,根据IFC四层架构体系,采用实体扩展和属性集扩展的方式,分层扩展了相应的构件实体、关系实... 现有IFC标准体系中,存在无法表达中国古建筑中特有的形制、结构、属性、艺术等特性的问题.基于UML,构建了斗栱、榫卯等古建筑结构的语义关系图,根据IFC四层架构体系,采用实体扩展和属性集扩展的方式,分层扩展了相应的构件实体、关系实体和属性集等,并以EXPRESS语言对扩展内容进行描述.通过使用IfcOpenShell工具包解析、VScode编辑的模型文件;并以Solibri和Blender为验证平台,检验IFC文件的可视化、可读性和实体与属性集信息的内容.根据验证结果,文中提出的古建筑领域的扩展符合IFC的扩展体系方法,对实现古建筑的结构和艺术等特性在信息模型中的交互和共享具有重要意义,可为古建筑工程勘察、修缮和运维的信息化保护提供强有力的支撑. 展开更多
关键词 木结构古建筑 信息化 工业基础类 语义 标准化 EXPRESS
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Application of Fair-faced Concrete Material in Architectural Design
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作者 LIU Wenyu 《Journal of Landscape Research》 2024年第2期77-80,86,共5页
With the development of the society,the function of the building also becomes rich from single refuge,and modern architects can’t satisfy the pursuit of traditional architectural form,and design goal is transformed i... With the development of the society,the function of the building also becomes rich from single refuge,and modern architects can’t satisfy the pursuit of traditional architectural form,and design goal is transformed into emphasizing the dialogue between architecture and people,architecture and emotions,and architecture and environment.As a kind of new building materials,fair-faced concrete expresses almost any emotion and meets people’s emotional needs with its excellent structural performance and good plasticity.In this paper,the characteristics,expressive force and application of fair-faced concrete are studied and analyzed,and the application and development prospect of fair-faced concrete in architectural design are summarized. 展开更多
关键词 Fair-faced concrete expressive force Architectural design Tadao Ando
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The Advertisement Translation Based on the Prototype Theory
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作者 XUE Hai-bin 《Sino-US English Teaching》 2024年第3期132-137,共6页
Based on the Prototype Theory,the prototypical feature of advertisement is found to be the combination of three language functions:the informative function,the expressive function,and the vocative function.The adverti... Based on the Prototype Theory,the prototypical feature of advertisement is found to be the combination of three language functions:the informative function,the expressive function,and the vocative function.The advertisement translation means the adjustment of the informative function and the expressive function according to the differences between languages or cultures in order to maximize the vocative function.The faithful translation is the closest to the prototype of the source text but not necessarily the best translation. 展开更多
关键词 the Prototype Theory ADVERTISEMENT the informative function the expressive function the vocative function
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Astrocytic endothelin-1 overexpression impairs learning and memory ability in ischemic stroke via altered hippocampal neurogenesis and lipid metabolism 被引量:3
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作者 Jie Li Wen Jiang +9 位作者 Yuefang Cai Zhenqiu Ning Yingying Zhou Chengyi Wang Sookja Ki Chung Yan Huang Jingbo Sun Minzhen Deng Lihua Zhou Xiao Cheng 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第3期650-656,共7页
Vascular etiology is the second most prevalent cause of cognitive impairment globally.Endothelin-1,which is produced and secreted by endothelial cells and astrocytes,is implicated in the pathogenesis of stroke.However... Vascular etiology is the second most prevalent cause of cognitive impairment globally.Endothelin-1,which is produced and secreted by endothelial cells and astrocytes,is implicated in the pathogenesis of stroke.However,the way in which changes in astrocytic endothelin-1 lead to poststroke cognitive deficits following transient middle cerebral artery occlusion is not well understood.Here,using mice in which astrocytic endothelin-1 was overexpressed,we found that the selective overexpression of endothelin-1 by astrocytic cells led to ischemic stroke-related dementia(1 hour of ischemia;7 days,28 days,or 3 months of reperfusion).We also revealed that astrocytic endothelin-1 overexpression contributed to the role of neural stem cell proliferation but impaired neurogenesis in the dentate gyrus of the hippocampus after middle cerebral artery occlusion.Comprehensive proteome profiles and western blot analysis confirmed that levels of glial fibrillary acidic protein and peroxiredoxin 6,which were differentially expressed in the brain,were significantly increased in mice with astrocytic endothelin-1 overexpression in comparison with wild-type mice 28 days after ischemic stroke.Moreover,the levels of the enriched differentially expressed proteins were closely related to lipid metabolism,as indicated by Kyoto Encyclopedia of Genes and Genomes pathway analysis.Liquid chromatography-mass spectrometry nontargeted metabolite profiling of brain tissues showed that astrocytic endothelin-1 overexpression altered lipid metabolism products such as glycerol phosphatidylcholine,sphingomyelin,and phosphatidic acid.Overall,this study demonstrates that astrocytic endothelin-1 overexpression can impair hippocampal neurogenesis and that it is correlated with lipid metabolism in poststroke cognitive dysfunction. 展开更多
关键词 astrocytic endothelin-1 dentate gyrus differentially expressed proteins HIPPOCAMPUS ischemic stroke learning and memory deficits lipid metabolism neural stem cells NEUROGENESIS proliferation
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基于PCIE的多嵌入式人工智能处理器低延迟数据交换技术
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作者 魏璇 温凯林 +3 位作者 李斌 刘淑涛 褚洁 蔡觉平 《电子科技》 2024年第5期32-37,46,共7页
针对多嵌入式人工智能(Artificial Intelligence,AI)处理器板卡之间的任务调度和数据交换冲突以及提高多板卡堆叠扩展时的可靠性和运行效率问题,文中提出了一种虫洞交换结构多嵌入式人工智能处理器高速数据交换技术和数据帧结构的解决... 针对多嵌入式人工智能(Artificial Intelligence,AI)处理器板卡之间的任务调度和数据交换冲突以及提高多板卡堆叠扩展时的可靠性和运行效率问题,文中提出了一种虫洞交换结构多嵌入式人工智能处理器高速数据交换技术和数据帧结构的解决方法。该方法基于PCIE(PCI Express)高速数据接口,将数据以数据单元的形式进行信息传递,并设计多重权重决策算法避免数据传输中的冲突,实现任务的并发多线程处理。搭建FPGA(Field Programmable Gate Array)平台进行设计和测试,结果表明PCIE的传输带宽利用效率达到了85%以上,数据交换延迟小于20μs,系统中断任务响应平均最大延迟时间为8.775μs。该技术适用于多处理器协同的高速交换电路,可扩展至混合PCIE和RapidIO交换电路结构。 展开更多
关键词 嵌入式人工智能处理器 数据交换 外围组件互连快速 PCI Express 交换开关 虫洞技术 数据仲裁 多重权重决策
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Smart prediction of liquefaction-induced lateral spreading 被引量:1
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作者 Muhammad Nouman Amjad Raja Tarek Abdoun Waleed El-Sekelly 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第6期2310-2325,共16页
The prediction of liquefaction-induced lateral spreading/displacement(Dh)is a challenging task for civil/geotechnical engineers.In this study,a new approach is proposed to predict Dh using gene expression programming(... The prediction of liquefaction-induced lateral spreading/displacement(Dh)is a challenging task for civil/geotechnical engineers.In this study,a new approach is proposed to predict Dh using gene expression programming(GEP).Based on statistical reasoning,individual models were developed for two topographies:free-face and gently sloping ground.Along with a comparison with conventional approaches for predicting the Dh,four additional regression-based soft computing models,i.e.Gaussian process regression(GPR),relevance vector machine(RVM),sequential minimal optimization regression(SMOR),and M5-tree,were developed and compared with the GEP model.The results indicate that the GEP models predict Dh with less bias,as evidenced by the root mean square error(RMSE)and mean absolute error(MAE)for training(i.e.1.092 and 0.815;and 0.643 and 0.526)and for testing(i.e.0.89 and 0.705;and 0.773 and 0.573)in free-face and gently sloping ground topographies,respectively.The overall performance for the free-face topology was ranked as follows:GEP>RVM>M5-tree>GPR>SMOR,with a total score of 40,32,24,15,and 10,respectively.For the gently sloping condition,the performance was ranked as follows:GEP>RVM>GPR>M5-tree>SMOR with a total score of 40,32,21,19,and 8,respectively.Finally,the results of the sensitivity analysis showed that for both free-face and gently sloping ground,the liquefiable layer thickness(T_(15))was the major parameter with percentage deterioration(%D)value of 99.15 and 90.72,respectively. 展开更多
关键词 Lateral spreading Intelligent modeling Gene expression programming(GEP) Closed-form solution Feature importance
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Knockdown of the atypical protein kinase genes GhABC1K2-A05 and GhABC1K12-A07 make cotton more sensitive to salt and PEG stress 被引量:1
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作者 Caixiang Wang Meili Li +3 位作者 Dingguo Zhang Xueli Zhang Juanjuan Liu Junji Su 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第10期3370-3386,共17页
Activity of bc1 complex kinase(ABC1K)is an atypical protein kinase(aPK)that plays a crucial role in plant mitochondrial and plastid stress responses,but little is known about the responses of ABC1Ks to stress in cotto... Activity of bc1 complex kinase(ABC1K)is an atypical protein kinase(aPK)that plays a crucial role in plant mitochondrial and plastid stress responses,but little is known about the responses of ABC1Ks to stress in cotton(Gossypium spp.).Here,we identified 40 ABC1Ks in upland cotton(Gossypium hirsutum L.)and found that the Gh ABC1Ks were unevenly distributed across 17 chromosomes.The GhABC1K family members included 35 paralogous gene pairs and were expanded by segmental duplication.The GhABC1K promoter sequences contained diverse cis-acting regulatory elements relevant to hormone or stress responses.The qRT-PCR results revealed that most Gh ABC1Ks were upregulated by exposure to different stresses.Gh ABC1K2-A05 and Gh ABC1K12-A07 expression levels were upregulated by at least three stress treatments.These genes were further functionally characterized by virus-induced gene silencing(VIGS).Compared with the controls,the Gh ABC1K2-A05-and Gh ABC1K12-A07-silenced cotton lines exhibited higher malondialdehyde(MDA)contents,lower catalase(CAT),peroxidase(POD)and superoxide dismutase(SOD)activities and reduced chlorophyll and soluble sugar contents under NaCl and PEG stress.In addition,the expression levels of six stress marker genes(Gh DREB2A,Gh SOS1,Gh CIPK6,Gh SOS2,Gh WRKY33,and Gh RD29A)were significantly downregulated after stress in the Gh ABC1K2-A05-and Gh ABC1K12-A07-silenced lines.The results indicate that knockdown of Gh ABC1K2-A05 and Gh ABC1K12-A07 make cotton more sensitive to salt and PEG stress.These findings can provide valuable information for intensive studies of Gh ABC1Ks in the responses and resistance of cotton to abiotic stresses. 展开更多
关键词 COTTON ABC1K abiotic stress responses expression patterns virus-induced gene silencing(VIGS)
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