目的:观察参芪泄浊饮对慢性肾衰大鼠肾组织胶原蛋白Ⅲ(ColⅢ)、胶原蛋白Ⅳ(ColⅣ)表达的影响。方法:采用Yokozawa法复制慢性肾衰大鼠模型,随机分为参芪泄浊饮高剂量组、常规剂量组、氯沙坦组、造模组及正常组,分别予参芪泄浊饮水煎液20...目的:观察参芪泄浊饮对慢性肾衰大鼠肾组织胶原蛋白Ⅲ(ColⅢ)、胶原蛋白Ⅳ(ColⅣ)表达的影响。方法:采用Yokozawa法复制慢性肾衰大鼠模型,随机分为参芪泄浊饮高剂量组、常规剂量组、氯沙坦组、造模组及正常组,分别予参芪泄浊饮水煎液20、10 m L·kg-1·d-1、氯沙坦9 mg·kg-1·d-1,造模组与正常组均予纯净水10 m L·kg-1·d-1,给药8周,酶法测量血肌酐(Scr)、尿素氮(BUN),并采用免疫组化检测肾脏ColⅢ、Ⅳ的表达。结果:1高剂量组Scr水平明显低于常规剂量组、氯沙坦组及造模组;常规剂量组与氯沙坦组无明显差别,均低于造模组;2ColⅢ表达水平:高剂量组<常规剂量组<氯沙坦组<造模组,相邻组间均存在显著统计差异(P<0.01);3ColⅣ表达水平除高剂量组与常规剂量组间不存在明显差异,其余变化趋势同ColⅢ。结论:参芪泄浊饮可通过减少细胞外基质ColⅢ、Ⅳ在肾组织过度沉积来缓解氮质血症,保护肾功能。展开更多
目的研究哮喘患者血清被动致敏后,细胞外信号调节激酶(extracellular signal regulate dkinase,ERK)磷酸化的变化,以探讨ERK信号传导途径在哮喘发病中的作用。方法免疫细胞化学法及免疫印记法检测ERK蛋白及其磷酸化(p-ERK)水平。结果哮...目的研究哮喘患者血清被动致敏后,细胞外信号调节激酶(extracellular signal regulate dkinase,ERK)磷酸化的变化,以探讨ERK信号传导途径在哮喘发病中的作用。方法免疫细胞化学法及免疫印记法检测ERK蛋白及其磷酸化(p-ERK)水平。结果哮喘患者血清被动致敏后,ERK蛋白的表达无明显变化,而p-ERK的表达明显升高(n=4,P<0.05)。结论哮喘患者血清被动致敏后,HASMCs的ERK磷酸化显著增强,此变化可能是哮喘形成和发生的机制之一。展开更多
With Bowytis cinerea and its extracellular macromolecular toxins as the test materials, 30 speiees of plants belonging to 29 genera and 21 families were selected as the test plants to observe the infectivity of B. dne...With Bowytis cinerea and its extracellular macromolecular toxins as the test materials, 30 speiees of plants belonging to 29 genera and 21 families were selected as the test plants to observe the infectivity of B. dnerea and damage status of macromolecular toxins secreted by B. cinerea on plants. The resulsts showed that 17 species of plants were beth infected by B. cinerea and damaged by toxins, accounting for 56.7% of the total plants. Two species of plants could be neither infected by B. cinerea nor damaged by toxins. The study provided the reference for further understanding of pathogenic mechanism of plant pathogenic fungi toxins.展开更多
Over last 20 years, extracellular matrices have been shown to be useful in promoting tissue regeneration. Recently, they have been used and have had success in achieving neurogenesis. Recent developments in extracellu...Over last 20 years, extracellular matrices have been shown to be useful in promoting tissue regeneration. Recently, they have been used and have had success in achieving neurogenesis. Recent developments in extracellular matrix design have allowed their successful in vivo incorporation to engender an environment favorable for neural regeneration in animal models. Promising treatments under investigation include manipulation of the intrinsic extracellular matrix and incorporation of engineered naometer-sized scaffolds through which inhibition of molecules serving as barriers to neuroregeneration and delivery of neurotrophic factors and/or cells for successful tissue regeneration can be achieved. Further understanding of the changes incurred within the extracellular matrix following central nervous system injury will undoubtedly help design a clinically efficacious extracellular matrix scaffold that can mitigate or reverse neural degeneration in the clinical setting.展开更多
文摘目的:观察参芪泄浊饮对慢性肾衰大鼠肾组织胶原蛋白Ⅲ(ColⅢ)、胶原蛋白Ⅳ(ColⅣ)表达的影响。方法:采用Yokozawa法复制慢性肾衰大鼠模型,随机分为参芪泄浊饮高剂量组、常规剂量组、氯沙坦组、造模组及正常组,分别予参芪泄浊饮水煎液20、10 m L·kg-1·d-1、氯沙坦9 mg·kg-1·d-1,造模组与正常组均予纯净水10 m L·kg-1·d-1,给药8周,酶法测量血肌酐(Scr)、尿素氮(BUN),并采用免疫组化检测肾脏ColⅢ、Ⅳ的表达。结果:1高剂量组Scr水平明显低于常规剂量组、氯沙坦组及造模组;常规剂量组与氯沙坦组无明显差别,均低于造模组;2ColⅢ表达水平:高剂量组<常规剂量组<氯沙坦组<造模组,相邻组间均存在显著统计差异(P<0.01);3ColⅣ表达水平除高剂量组与常规剂量组间不存在明显差异,其余变化趋势同ColⅢ。结论:参芪泄浊饮可通过减少细胞外基质ColⅢ、Ⅳ在肾组织过度沉积来缓解氮质血症,保护肾功能。
文摘目的研究哮喘患者血清被动致敏后,细胞外信号调节激酶(extracellular signal regulate dkinase,ERK)磷酸化的变化,以探讨ERK信号传导途径在哮喘发病中的作用。方法免疫细胞化学法及免疫印记法检测ERK蛋白及其磷酸化(p-ERK)水平。结果哮喘患者血清被动致敏后,ERK蛋白的表达无明显变化,而p-ERK的表达明显升高(n=4,P<0.05)。结论哮喘患者血清被动致敏后,HASMCs的ERK磷酸化显著增强,此变化可能是哮喘形成和发生的机制之一。
基金Supported by National Natural Science Foundation of China(31260062,30860121)Talent Introduction Project of Kunming University(YJL11017)
文摘With Bowytis cinerea and its extracellular macromolecular toxins as the test materials, 30 speiees of plants belonging to 29 genera and 21 families were selected as the test plants to observe the infectivity of B. dnerea and damage status of macromolecular toxins secreted by B. cinerea on plants. The resulsts showed that 17 species of plants were beth infected by B. cinerea and damaged by toxins, accounting for 56.7% of the total plants. Two species of plants could be neither infected by B. cinerea nor damaged by toxins. The study provided the reference for further understanding of pathogenic mechanism of plant pathogenic fungi toxins.
文摘Over last 20 years, extracellular matrices have been shown to be useful in promoting tissue regeneration. Recently, they have been used and have had success in achieving neurogenesis. Recent developments in extracellular matrix design have allowed their successful in vivo incorporation to engender an environment favorable for neural regeneration in animal models. Promising treatments under investigation include manipulation of the intrinsic extracellular matrix and incorporation of engineered naometer-sized scaffolds through which inhibition of molecules serving as barriers to neuroregeneration and delivery of neurotrophic factors and/or cells for successful tissue regeneration can be achieved. Further understanding of the changes incurred within the extracellular matrix following central nervous system injury will undoubtedly help design a clinically efficacious extracellular matrix scaffold that can mitigate or reverse neural degeneration in the clinical setting.
