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Extracting water body data based on SDWI and threshold segmentation:A case study in permafrost area surrounding Salt Lake in Hoh Xil,Qinghai-Tibet Plateau,China
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作者 QingSong Du GuoYu Li +4 位作者 Dun Chen ShunShun Qi Yu Zhou Fei Wang YaPeng Cao 《Research in Cold and Arid Regions》 CSCD 2023年第4期202-209,共8页
There are a large number of lakes,rivers,and other natural water bodies distributed in the permafrost area of the Qinghai-Tibet Plateau(QTP).The changes in water bodies will affect the distribution of water resources ... There are a large number of lakes,rivers,and other natural water bodies distributed in the permafrost area of the Qinghai-Tibet Plateau(QTP).The changes in water bodies will affect the distribution of water resources in sur-rounding areas and downstream areas,resulting in environmental impact and bringing potential flood disasters,which will induce more serious issues and problems in alpine and high-altitude areas with a fragile habitat(such as the QTP in China).Generally,effective,reasonable,and scientific monitoring of large-scale water bodies can not only document the changes in water bodies intuitively,but also provide important theoretical reference for subsequent environmental impact prediction,and disaster prevention and mitigation in due course of time.The large-scale water extraction technology derived from the optical remote sensing(RS)image is seriously affected by clouds,bringing about large differences among the extracted water result products.Synthetic aperture radar(SAR)RS technology has the unique advantage characteristics of all-weather,all-day,strong penetration,and not being affected by clouds,which is hopeful in extracting water body data,especially for days with cloudy weather.The data extraction of large-scale water bodies based on SAR images can effectively avoid the errors caused by clouds that become prevalent at present.In this paper,the Hoh Xil Salt Lake on the QTP and its surrounding five lakes are taken as the research objects.The 2-scene Sentinel-1 SAR image data covering the whole area on 22 August 2022 was used to verify the feasibility of extracting water body data in permafrost zones.Furthermore,on 22 August 2022,the wealth here was cloudy,which made the optical RS images,e.g.,Sentinel-2 images full of clouds.The results show that:using the Sentinel-1 image and threshold segmentation method to extract water body data is efficient and effective with excellent results in permafrost areas.Concretely,the Sentinel-1 dual-polarized water index(SDWI),calculated by combining dual vertical–vertical(VV)polarized and verti-cal–horizontal(VH)polarized data is a useful index for water extraction and the result is better than each of the VV or VH polarized images. 展开更多
关键词 Permafrost region Water body extraction Salt Lake in Hoh Xil Qinghai-Tibet Plateau Sentinel-1 Ecological environment impact Disaster prevention and mitigation
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On Some Problems of Extracting the Root from a Given Finite Language
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作者 Boris F. Melnikov Aleksandra A. Melnikova 《Journal of Applied Mathematics and Physics》 2023年第5期1471-1481,共11页
Based on the standard definition of the product (concatenation), the natural non-negative degree of the language is introduced. Root extraction is the reverse operation to it, and it can be defined in several differen... Based on the standard definition of the product (concatenation), the natural non-negative degree of the language is introduced. Root extraction is the reverse operation to it, and it can be defined in several different ways. Despite the simplicity of the formulation of the problem of extracting the root, the authors could not find any description of it in the literature (as well as on the Internet), including even its formulation. Most of the material in this article is devoted to the simplest version of the formulation: the root of the 2<sup>nd</sup> degree for the 1-letter alphabet, but many of the provisions of the article are generalized to more complex cases. Apparently, for a possible future description of a polynomial algorithm for solving at least one of the described statements of root extraction problems, it is first necessary to really analyze in detail such a special case, that is: either describe the necessary polynomial algorithm, or, conversely, show that the problem belongs to the class of NP-complete problems. Thus, in this article, we do not propose a polynomial algorithm for the problems under consideration;however, the models described here should help in constructing appropriate heuristic algorithms for their solution. A detailed description of the possible further application of such heuristic algorithms is beyond the scope of this article. . 展开更多
关键词 Formal Languages Iterations of Languages Root Extraction Algorithms The Boolean
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Extracting vanadium from stone-coal by oxygen pressure acid leaching and solvent extraction 被引量:15
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作者 邓志敢 魏昶 +3 位作者 樊刚 李旻廷 李存兄 李兴彬 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2010年第S1期118-122,共5页
Vanadium extraction from stone-coal was investigated by oxygen pressure acid leaching and solvent extraction.The mineralogy of the stone-coal from Tongren City of Guizhou Province,China,was investigated by various det... Vanadium extraction from stone-coal was investigated by oxygen pressure acid leaching and solvent extraction.The mineralogy of the stone-coal from Tongren City of Guizhou Province,China,was investigated by various determination methods. The effects of leaching time,leaching temperature,leaching agent concentration,leaching L/S ratio,granularity of material,additive consumption were investigated based on the mineralogy.The results show that under the conditions of leaching time of 3-4 h, temperature of 150℃,sulfuric acid consumption of 25%?30%,ratio of liquid to solid of 1.2:1,the granularity less than 0.074 mm, additive consumption of 3%-5%,and oxygen pressure of 1.2 MPa,and the vanadium leaching rate can be more than 92%by the method of two-step pressurized acid leaching.The powdery V2O5 product with 99.52%in V2O5 content is obtained by the flowsheet of acid recovery,removing iron by reduction process,solvent extraction,precipitating vanadium with ammonium water,and pyrolysis from the stone-coal oxygen pressure acid-leaching solution.The total recovery efficiency of vanadium is above 85%,which is more than 20%higher than that obtained in the conventional process.Furthermore,the new process does not cause air pollution since no HCl or Cl2 is released by calcination of the raw material. 展开更多
关键词 stone-coal extracting vanadium oxygen pressure acid leaching acid recovery solvent extraction
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Information-Theoretic Analysis for the Difficulty of Extracting Hidden Information 被引量:5
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作者 ZHANGWei-ming LIShi-qu +1 位作者 CAOJia LIUJiu-fen 《Wuhan University Journal of Natural Sciences》 EI CAS 2005年第1期315-318,共4页
The difficulty of extracting hidden information, which is essentially a kindof secrecy, is analyzed by information-theoretic method. The relations between key rate, messagerate, hiding capacity and difficulty of extra... The difficulty of extracting hidden information, which is essentially a kindof secrecy, is analyzed by information-theoretic method. The relations between key rate, messagerate, hiding capacity and difficulty of extraction are studied in the terms of unicity distance ofstego-key, and the theoretic conclusion is used to analyze the actual extracting attack on LeastSignificant Bit(LSB) steganographic algorithms. 展开更多
关键词 information hiding STEGANOGRAPHY extracting attack difficulty ofextraction unicity distance
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AUTO-EXTRACTING TECHNIQUE OF DYNAMIC CHAOS FEATURES FOR NONLINEAR TIME SERIES 被引量:6
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作者 CHEN Guo 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2006年第4期524-529,共6页
The main purpose of nonlinear time series analysis is based on the rebuilding theory of phase space, and to study how to transform the response signal to rebuilt phase space in order to extract dynamic feature informa... The main purpose of nonlinear time series analysis is based on the rebuilding theory of phase space, and to study how to transform the response signal to rebuilt phase space in order to extract dynamic feature information, and to provide effective approach for nonlinear signal analysis and fault diagnosis of nonlinear dynamic system. Now, it has already formed an important offset of nonlinear science. But, traditional method cannot extract chaos features automatically, and it needs man's participation in the whole process. A new method is put forward, which can implement auto-extracting of chaos features for nonlinear time series. Firstly, to confirm time delay r by autocorrelation method; Secondly, to compute embedded dimension m and correlation dimension D; Thirdly, to compute the maximum Lyapunov index λmax; Finally, to calculate the chaos degree Dch of Poincare map, and the non-circle degree Dnc and non-order degree Dno of quasi-phase orbit. Chaos features extracting has important meaning to fault diagnosis of nonlinear system based on nonlinear chaos features. Examples show validity of the proposed method. 展开更多
关键词 Nonlinear time series analysis Chaos Feature extracting Fault diagnosis
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Extracting intraocular foreign body at or near the ciliary body with scleral indentation in direct visualization 被引量:1
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作者 Jiang-Dong Ji Yu-Zhi Ding +3 位作者 Zi-Zhong Hu Ping Xie Song-Tao Yuan Qing-Huai Liu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2018年第3期533-535,共3页
Dear Editor,I'm Dr. Qing-Huai Liu from Department of Ophthalmology, the First Affiliated Hospital of Nanjing Medical University,Nanjing, China. I write to present four cases diagnosed with the intraocular foreign bo... Dear Editor,I'm Dr. Qing-Huai Liu from Department of Ophthalmology, the First Affiliated Hospital of Nanjing Medical University,Nanjing, China. I write to present four cases diagnosed with the intraocular foreign body (IOFB) at or near the ciliary body and to evaluate the effect of extracting IOFB with scleral indentation in direct visualization. 展开更多
关键词 extracting intraocular foreign body at or near the ciliary body scleral indentation direct visualization
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Minimum Amount of Extracting Solvent of a Separation of Two Rare Earth Components 被引量:1
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作者 Fuxiang Cheng Sheng Wu +4 位作者 Yan Liu Songling Wang Bo Zhang Chunsheng Liao Chunhua Yan 《Advances in Materials Physics and Chemistry》 2014年第12期275-283,共9页
A significant development in the theory of countercurrent extraction will be presented in this article. New expressions of the term in countercurrent extraction process analysis, “Adjacent Stage Impurity Ratio” (ASI... A significant development in the theory of countercurrent extraction will be presented in this article. New expressions of the term in countercurrent extraction process analysis, “Adjacent Stage Impurity Ratio” (ASIR), are deduced. Furthermore, based on the term together with mass balance and extraction equilibrium, the conditions where a given countercurrent extraction separation operation can have minimum amounts of both extracting solvent and scrubbing agent solution can be estimated, and the equations of the two minimum amounts can be deduced. It was found that the equations for a two-component separation using a single aqueous or organic feed are exactly the same as they appeared in the theory initially established in 1970s. Unlike its earlier version, the present derivation does not involve feed-stage-composition hypothesis, and also has the advantage of dealing with a double-feed system where both aqueous and organic feeds are simultaneously employed whereas the earlier theory can only analyze a separation using a single aqueous or organic feed. 展开更多
关键词 Theory of Countercurrent Extraction Minimum AMOUNT of extracting SOLVENT TWO-COMPONENT SEPARATION Adjacent Stage IMPURITY Ratio
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REFINING OF SULFATE SOAP AND EXTRACTING OF PHYTOSTEROLS
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作者 Jin Qi Liu Zhiqun Northeast Forestry University 《Journal of Northeast Forestry University》 SCIE CAS CSCD 1992年第1期82-88,共7页
The sulfatc soap is refined through extracting unsaponifiable neutral substance using ethyl acetate and hcxane-acetone. The content of neutral substance in tall oil decreases from 32.8% to 17.4% and 18%. The acid numb... The sulfatc soap is refined through extracting unsaponifiable neutral substance using ethyl acetate and hcxane-acetone. The content of neutral substance in tall oil decreases from 32.8% to 17.4% and 18%. The acid number of tall oil increases from 119 to 169 and 153. The quality of tall oil is greatly improved. This provides a favorable condition for distillation of tall oil. The unsaponifiable neutral substance from crude sulfate soap mainly consists of tcrpene aldehydes, tcrpcnc alcohols, fatty alcohols and stcrols in which larixol is 26.3% and 18. 5%, stcrols arc 17% and 27.1% separately. The white crystals containing stcrols of 96.9% are obtained by crystallization and rccrystallization. The stcrols arc mainly β-sitostcrol and campcsterol. 展开更多
关键词 REFINING Sulfatc soap extracting PHYTOSTEROL
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PARAMETER IDENTIFICATION IN OFFSHORE PLATFORM USING ARMA MODEL AND TECHNOLOGY OF EXTRACTING FREE VIBRATION SIGNAL
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作者 欧进萍 何林 肖仪清 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI 2003年第4期449-457,共9页
A procedure for identifying the dynamic parameter of offshore platform is presented. The present procedure consists of two key features. First uses random decrement (RD) technology to extract free vibration signal in ... A procedure for identifying the dynamic parameter of offshore platform is presented. The present procedure consists of two key features. First uses random decrement (RD) technology to extract free vibration signal in strong noise environment in which it may not white noise. Second technology which called autoregressive moving average (ARMA) was used to model the data treated by the random decrement method. In order to get rid of the color noise in the output signal response from the offshore platform an imaginary system is added in RD system and make the course of extracting performed under the state of color input by choosing the breakover condition and lead time. For eliminating multi_values of parameters identified, an updating moving average method is used. The dynamic parameters of structure under arbitrary input are identified. Example of the method as applied to a scale_model offshore platform was used to evaluate the technology of efficiency and the value of on_line. 展开更多
关键词 offshore platform structure ARMA extracting free vibration signal MA parameter updating parameters identification
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Comparison of Three Methods in Extracting Coherent Modes from a Doppler Backscatter System
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作者 张小辉 刘阿娣 +7 位作者 周楚 胡健强 王明远 俞昌旋 刘万东 李弘 兰涛 谢锦林 《Chinese Physics Letters》 SCIE CAS CSCD 2015年第12期92-95,共4页
We compare three different methods to extract coherent modes from Doppler backscattering (DBS), which are center of gravity (COG) of the complex amplitude spectrum, spectrum of DBS phase derivative (phase derivat... We compare three different methods to extract coherent modes from Doppler backscattering (DBS), which are center of gravity (COG) of the complex amplitude spectrum, spectrum of DBS phase derivative (phase derivative method), and phase spectrum, respectively. These three methods are all feasible to extract coherent modes, for example, geodesic acoustic mode oscillation. However, there are stilI differences between dealing with high frequency modes (several hundred kHz) and low frequency modes, (several kHz) hiding in DBS signal. There is a significant amount of power at low frequencies in the phase spectrum, which can be removed by using the phase derivative method and COG. High frequency modes are clearer by using the COO and the phase derivative method than the phase spectrum. The spectrum of DBS amplitude does not show the coherent modes detected by using COG, phase derivative method and phase spectrum. When two Doppler shifted peaks exist, coherent modes and their harmonics appear in the spectrum of DBS amplitude, which are introduced by the DBS phase. 展开更多
关键词 DBS Comparison of Three Methods in extracting Coherent Modes from a Doppler Backscatter System COG Figure
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Environmentally sound timber extracting techniques for small tree harvesting
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作者 王立海 《Journal of Forestry Research》 SCIE CAS CSCD 2000年第4期269-272,共4页
Due to large area disturbed and great deal of energy cost during skidding operations, introducing or applying the appropriate techniques of timber extracting could significantly reduce the impact of timber extraction ... Due to large area disturbed and great deal of energy cost during skidding operations, introducing or applying the appropriate techniques of timber extracting could significantly reduce the impact of timber extraction operations to forest environment while pursuing the reasonable operation costs. Four environmentally sound timber extraction techniques for small tree harvesting, particularly for thinning operations, were presented and introduced in this paper. The results of evaluation, test or practices indicated that these timber-extracting techniques are feasible, applicable and reasonable in small tree harvesting with a relatively low impact no environment and a moderate operation cost. 展开更多
关键词 Timber extracting Low environmental impact Small tree harvesting
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Study on the surface of hydrous titanium oxide adsorbent for extracting uranium from seawater-Ⅱ. The surface of HTO
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作者 Chen Huizhen and Jiang Anbei Third Institute of Oceanography, State Oceanic Administration, Xiamen, ChinaLanzhou Institute of Chemical Physics, Academia Sinica, Lanzhou, China Present address: Analysis and Testing Centre, Xiamen University, Xiamen, China 《Acta Oceanologica Sinica》 SCIE CAS CSCD 1991年第1期83-92,共10页
This is second paper summarizing the study on the hydrous titanium oxide absorbent for extracting uranium fromseawater. The investigation is performed by means of X- ray photoelectronic energy spectroscopy for chemica... This is second paper summarizing the study on the hydrous titanium oxide absorbent for extracting uranium fromseawater. The investigation is performed by means of X- ray photoelectronic energy spectroscopy for chemical analysis ( ESC A ) , determination of surface hydroxy radical, Fourier-transfer infrared spectrophotometry (FT-IR ) , electron paramagnetic resonance (EPR), inductively coupled Plasma torch (ICP), etc. The emphasis is laid upon the exploration of HTO surface and a discussion about the adsorption micromechanism. 展开更多
关键词 In Study on the surface of hydrous titanium oxide adsorbent for extracting uranium from seawater The surface of HTO
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True Random Number Generator Realized by Extracting Entropy from a Negative-Inductance Superconducting Quantum Interference Device
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作者 李浩 刘建设 +4 位作者 蔡涵 张颖珊 刘其春 李刚 陈炜 《Chinese Physics Letters》 SCIE CAS CSCD 2017年第1期129-132,共4页
A new type of superconductive true random number generator (TRNG) based on a negative-inductance superconducting quantum interference device (nSQUID) is proposed. The entropy harnessed to generate random numbers comes... A new type of superconductive true random number generator (TRNG) based on a negative-inductance superconducting quantum interference device (nSQUID) is proposed. The entropy harnessed to generate random numbers comes from the phenomenon of symmetry breaking in the nSQUID. The experimental circuit is fabricated by the Nb-based lift-off process. Low-temperature tests of the circuit verify the basic function of the proposed TRNG. The frequency characteristics of the TRNG have been analyzed by simulation. The generation rate of random numbers is expected to achieve hundreds of megahertz to tens of gigahertz. 展开更多
关键词 True Random Number Generator Realized by extracting Entropy from a Negative-Inductance Superconducting Quantum Interference Device
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A NEW METHOD FOR EXTRACTING CHARACTERISTIC SIGNAL IN EPILEPTIC EEG
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作者 Yuan Xu Dezhong Yao(University of Electronic Science and Technology of China, Chengdu 610054) 《Chinese Journal of Biomedical Engineering(English Edition)》 1999年第3期41-42,共2页
关键词 A NEW METHOD FOR extracting CHARACTERISTIC SIGNAL IN EPILEPTIC EEG BME
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A new process of extracting vanadium from stone coal 被引量:9
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作者 Yan-hua Liu Chao Yang +1 位作者 Pei-you Li Shi-qi Li 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2010年第4期381-388,共8页
A new process of extracting vanadium from the stone coal vanadium ore in Fangshankou, Dtmhuang area of Gansu Province, China was introduced. Various leaching experiments were carried out, and the results show that the... A new process of extracting vanadium from the stone coal vanadium ore in Fangshankou, Dtmhuang area of Gansu Province, China was introduced. Various leaching experiments were carried out, and the results show that the vanadium ore in Fangshankou is difficult to process due to its high consumption of acid and the high leaching rate of impurities. However, the leaching rate can be up to 80% and the content of V2O5 in the residue can be between 0.22%-0.25% in the process of ore fme grinding→oxidation roasting→mixing and ripening→aqueous leaching→P2O4 solvent extraction→sulfuric acid stripping→oxidation and precipitation→decomposition by heat. Also, the quality of flaky V205 produced by this process can meet the requirements of GB3283--87. The total leaching rate of vanadium is 70%. Also, three types of wastes are easy to treat. The vanadium extraction process is better in relation to the aspect of environmental protection than the sodium method. 展开更多
关键词 stone coal VANADIUM EXTRACTION LEACHING solvent extraction V2O5
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Thermodynamics and kinetics of extracting zinc from zinc oxide ore by the ammonium sulfate roasting method 被引量:15
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作者 Yi Sun Xiao-yi Shen Yu-chun Zhai 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2015年第5期467-475,共9页
Thermodynamic analyses and kinetic studies were performed on zinc oxide ore treatment by (NH4)2SO4 roasting technology. The results show that it is theoretically feasible to realize a roasting reaction between the z... Thermodynamic analyses and kinetic studies were performed on zinc oxide ore treatment by (NH4)2SO4 roasting technology. The results show that it is theoretically feasible to realize a roasting reaction between the zinc oxide ore and (NH4)2SO4 in a temperature range of 573-723 K. The effects of reaction temperature and particle size on the extraction rate of zinc were also examined. It is found that a surface chemical reaction is the rate-controlling step in roasting kinetics. The calculated activation energy of this process is about 45.57 kJ/mol, and the kinetic model can be expressed as follows: 1 - (1 - α)1/3 = 30.85 exp(-45.57/RT)·t. An extraction ratio of zinc as high as 92% could be achieved under the optimum conditions. 展开更多
关键词 zinc metallurgy extraction ammonium sulfate ore roasting THERMODYNAMICS KINETICS
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Extracting copper from copper oxide ore by a zwitterionic reagent and dissolution kinetics 被引量:7
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作者 Jiu-shuai Deng Shu-ming Wen +1 位作者 Jian-ying Deng Dan-dan Wu 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2015年第3期241-248,共8页
Sulfamic acid (SA), which possesses a zwitterionic structure, was applied as a leaching reagent for the first time for extracting copper from copper oxide ore. The effects of reaction time, temperature, particle siz... Sulfamic acid (SA), which possesses a zwitterionic structure, was applied as a leaching reagent for the first time for extracting copper from copper oxide ore. The effects of reaction time, temperature, particle size, reagent concentration, and stirring speed on this leach- ing were studied. The dissolution kinetics of malachite was illustrated with a three-dimensional diffusion model. A novel leaching effect of SA on malachite was eventually demonstrated. The leaching rate increased with decreasing particle size and increasing concentration, reac- tion temperature and stirring speed. The activation energy for SA leaching malachite was 33.23 kJ/mol. Furthermore, the effectiveness of SA as a new reagent for extracting copper from copper oxide ore was confirmed by experiment. This approach may provide a solution suitable for subsequent electrowinning. In addition, results reported herein may provide basic data that enable the leaching of other carbonate miner- als of copper, zinc, cobalt and so on in an SA system. 展开更多
关键词 MALACHITE COPPER extraction sulfamic acid DISSOLUTION kinetics
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Algorithmic Foundation and Software Tools for Extracting Shoreline Features from Remote Sensing Imagery and LiDAR Data 被引量:8
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作者 Hongxing Liu Lei Wang +2 位作者 Douglas J. Sherman Qiusheng Wu Haibin Su 《Journal of Geographic Information System》 2011年第2期99-119,共21页
This paper presents algorithmic components and corresponding software routines for extracting shoreline features from remote sensing imagery and LiDAR data. Conceptually, shoreline features are treated as boundary lin... This paper presents algorithmic components and corresponding software routines for extracting shoreline features from remote sensing imagery and LiDAR data. Conceptually, shoreline features are treated as boundary lines between land objects and water objects. Numerical algorithms have been identified and de-vised to segment and classify remote sensing imagery and LiDAR data into land and water pixels, to form and enhance land and water objects, and to trace and vectorize the boundaries between land and water ob-jects as shoreline features. A contouring routine is developed as an alternative method for extracting shore-line features from LiDAR data. While most of numerical algorithms are implemented using C++ program-ming language, some algorithms use available functions of ArcObjects in ArcGIS. Based on VB .NET and ArcObjects programming, a graphical user’s interface has been developed to integrate and organize shoreline extraction routines into a software package. This product represents the first comprehensive software tool dedicated for extracting shorelines from remotely sensed data. Radarsat SAR image, QuickBird multispectral image, and airborne LiDAR data have been used to demonstrate how these software routines can be utilized and combined to extract shoreline features from different types of input data sources: panchromatic or single band imagery, color or multi-spectral image, and LiDAR elevation data. Our software package is freely available for the public through the internet. 展开更多
关键词 SHORELINE Extraction Remote Sensing IMAGERY LiDAR Data ArcGIS ARCOBJECTS VB.NET
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New templates of CNN for extracting corners of objects in gray-scale images 被引量:3
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作者 Lequan Min, Ming Lei, and Xisong DongApplied Science School, University of Science and Technology Beijing, Beijing 100083, China 《Journal of University of Science and Technology Beijing》 CSCD 2003年第3期73-75,共3页
A new cellular neural network (CNN) with nonlinear templates is presented forextracting convex corners of objects in gray-scale images. Application examples showed that the newCNN can even detect convex corner charact... A new cellular neural network (CNN) with nonlinear templates is presented forextracting convex corners of objects in gray-scale images. Application examples showed that the newCNN can even detect convex corner characteristics of objects in images with Gaussian noise. 展开更多
关键词 cellular neural network extract convex comers gray-scale images
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Comparison of methods for extracting high-throughput sequencing RNA from Korean pine seeds 被引量:1
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作者 Yan Liang Hai-long Shen +2 位作者 Chun-ping Liu Ling Yang Peng Zhang 《Journal of Forestry Research》 SCIE CAS CSCD 2016年第1期33-40,共8页
Korean pine (Pinus koraiensis Sieb. et Zucc.) is an ecologically and economically important tree species in East Asia. Molecular studies of seed development of this species are limited due to the lack of effective R... Korean pine (Pinus koraiensis Sieb. et Zucc.) is an ecologically and economically important tree species in East Asia. Molecular studies of seed development of this species are limited due to the lack of effective RNA extraction protocols. This study aimed to obtain an effec- tive method to extract high-quality RNA from Korean pine seeds. The TRIzol kit and CTAB methods were used to extract the total RNA from Korean pine seeds at different developmental stages. The bands of RNA extracted by CTAB were not clear, whereas the bands of RNA extracted by the TRIzol kit were brighter and clearer, indicating higher quality and integrity of the RNA products extracted by the TRIzol kit. The 28S rRNA band was approximately 1.5- to 2-fold brighter than the 18S rRNA band on the agarose gel electrophoresis. The absorbance value A 260/280 was 1.8-2.0, and the absorbance value A 260/230 was 〉1.9. The Bioanalyzer RNA integrity results showed that the RNA integrity number of the RNA extracted using the TR/zol kit was acceptable for high-throughputsequencing. Therefore, the total RNA extracted using the TRIzol kit method can be used for high-throughput sequencing and other molecular biology experiments. 展开更多
关键词 Pinus koraiensis SEEDS RNA extraction CTAB TRIzol kit
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