目的:克隆粉尘螨过敏原Der f 11基因,表达、纯化该蛋白,并鉴定其免疫活性。方法:人工合成粉尘螨第11组过敏原Der f 11基因,将其连接至pET-32a表达载体,用异丙基-β-D-硫代半乳糖苷(IPTG)诱导表达后,通过Ni+亲和层析纯化重组过敏原Der f...目的:克隆粉尘螨过敏原Der f 11基因,表达、纯化该蛋白,并鉴定其免疫活性。方法:人工合成粉尘螨第11组过敏原Der f 11基因,将其连接至pET-32a表达载体,用异丙基-β-D-硫代半乳糖苷(IPTG)诱导表达后,通过Ni+亲和层析纯化重组过敏原Der f 11。以粉尘螨过敏患者血清作为一抗,经Western blot方法分析Der f 11的免疫学特性。结果:获得高纯度的重组Der f 11蛋白,SDS-PAGE结果显示表达产物pET-32a(+)-Der f 11分子质量约为118 ku。重组过敏原Der f 11检测15份尘螨过敏性患者血清中特异性IgE,阳性率为20%。结论:获得的Der f 11重组蛋白具有与天然蛋白相似的免疫学活性,为标准化抗原的临床特异性诊断和治疗及进一步的实验研究奠定基础。展开更多
-<sup>11</sup>C and <sup>17</sup>F secondary beams have been successfully tuned on the CIAE sec-ondary radioactive beam line at the HI-13 tandem accelerator.The setting parametersof the seconda...-<sup>11</sup>C and <sup>17</sup>F secondary beams have been successfully tuned on the CIAE sec-ondary radioactive beam line at the HI-13 tandem accelerator.The setting parametersof the secondary beam line and the beam quality for <sup>11</sup>C and <sup>17</sup>F are given.A <sup>11</sup>C beamis produced with intensity 1.2×10<sup>5</sup> particles/s and energy 41±1.0 MeV.展开更多
Primary hyperparathyroidism (PHPT) is a relative common medical problem caused by the inappropriate secretion of parathyroid hormone (PTH) by one or more parathyroid glands. The diagnosis is established by serum calci...Primary hyperparathyroidism (PHPT) is a relative common medical problem caused by the inappropriate secretion of parathyroid hormone (PTH) by one or more parathyroid glands. The diagnosis is established by serum calcium and PTH levels and once the diagnosis is established imaging studies help localize the hyperfunctioning adenoma in preparation for curative surgery. Until now, the imaging studies most commonly utilized in PHPT are ultrasonography and <sup>99m</sup>Tc-Sesta-methoxyisobutylisonitrile (MIBI) parathyroid scintigraphy. However, these studies often fail to localize the adenoma and inappropriately delay patient referral to a potentially curative surgery. We present the case of a 64-year-old female with symptomatic PHPT who had 3 negative <sup>99m</sup>Tc-Sestamibi Scans over a period of 5 years who eventually had a PET/CT with <sup>11</sup>C-Choline that identified a right lower parathyroid adenoma. She underwent a right lower parathyroidectomy and had a successful outcome. We present a review the current imaging techniques used in the management of PHPT including <sup>99m</sup>Tc-Sesta-MIBI scintigraphy and its limitations and novel use of PET/CT with <sup>11</sup>C-Choline and <sup>18</sup>F-Choline in this disease and emphasize the fact that, according to current guidelines, failure to localize the adenoma should not delay referral for curative surgery.展开更多
文摘目的:克隆粉尘螨过敏原Der f 11基因,表达、纯化该蛋白,并鉴定其免疫活性。方法:人工合成粉尘螨第11组过敏原Der f 11基因,将其连接至pET-32a表达载体,用异丙基-β-D-硫代半乳糖苷(IPTG)诱导表达后,通过Ni+亲和层析纯化重组过敏原Der f 11。以粉尘螨过敏患者血清作为一抗,经Western blot方法分析Der f 11的免疫学特性。结果:获得高纯度的重组Der f 11蛋白,SDS-PAGE结果显示表达产物pET-32a(+)-Der f 11分子质量约为118 ku。重组过敏原Der f 11检测15份尘螨过敏性患者血清中特异性IgE,阳性率为20%。结论:获得的Der f 11重组蛋白具有与天然蛋白相似的免疫学活性,为标准化抗原的临床特异性诊断和治疗及进一步的实验研究奠定基础。
基金The project supported in part by National Natural Science Foundation of ChinaChina Nuclear Industry Science Foundation.
文摘-<sup>11</sup>C and <sup>17</sup>F secondary beams have been successfully tuned on the CIAE sec-ondary radioactive beam line at the HI-13 tandem accelerator.The setting parametersof the secondary beam line and the beam quality for <sup>11</sup>C and <sup>17</sup>F are given.A <sup>11</sup>C beamis produced with intensity 1.2×10<sup>5</sup> particles/s and energy 41±1.0 MeV.
文摘Primary hyperparathyroidism (PHPT) is a relative common medical problem caused by the inappropriate secretion of parathyroid hormone (PTH) by one or more parathyroid glands. The diagnosis is established by serum calcium and PTH levels and once the diagnosis is established imaging studies help localize the hyperfunctioning adenoma in preparation for curative surgery. Until now, the imaging studies most commonly utilized in PHPT are ultrasonography and <sup>99m</sup>Tc-Sesta-methoxyisobutylisonitrile (MIBI) parathyroid scintigraphy. However, these studies often fail to localize the adenoma and inappropriately delay patient referral to a potentially curative surgery. We present the case of a 64-year-old female with symptomatic PHPT who had 3 negative <sup>99m</sup>Tc-Sestamibi Scans over a period of 5 years who eventually had a PET/CT with <sup>11</sup>C-Choline that identified a right lower parathyroid adenoma. She underwent a right lower parathyroidectomy and had a successful outcome. We present a review the current imaging techniques used in the management of PHPT including <sup>99m</sup>Tc-Sesta-MIBI scintigraphy and its limitations and novel use of PET/CT with <sup>11</sup>C-Choline and <sup>18</sup>F-Choline in this disease and emphasize the fact that, according to current guidelines, failure to localize the adenoma should not delay referral for curative surgery.
