Background PB1-F2 protein has been proven to increase the pathogenicity of influenza A virus （IAV） strains in primary infection and in secondary bacterial infection. It can also regulate the activity of viral polyme...Background PB1-F2 protein has been proven to increase the pathogenicity of influenza A virus （IAV） strains in primary infection and in secondary bacterial infection. It can also regulate the activity of viral polymerase. However, it was shown in another retrospective study that a portion of IAVs do not express full-length PB1-F2 protein during virus development; different kinds of stop codons cause exits in the open reading frames and form PB1-F2 gene products with the corresponding genotypes. Truncated PB1-F2 in human H3N2 IAVs has long been detected in North America but its evolution in China is still unclear. Methods Influenza-like illnesses （ILls） from the whole of Jiangsu Province were collected and inspected to determine the type and subtype of the viruses. A portion of isolates collected in the epidemic period were selected as samples for later whole-genome sequencing, and the exact sequences were determined and analyzed. Results H3N2 influenza virus was one of the epidemical strains which had been prevalent during 2009-2010, in Jiangsu. Five H3N2 isolates with truncated PB1-F2 protein （25aa） were detected in influenza samples from Nanjing and Xuzhou, while seven similar H3N2 isolates were also reported in Niigata, Japan. Conclusion This emergence indicates the possibility that there has been transmission of the H3N2 virus between the two countries.展开更多
基金This work was supported by grants from the Natural Science Foundation of Jiangsu Province （No. BK20131450）, Jiangsu Province Key Medical Talent Foundation （No. RC2011084） and National Natural Science Foundation of China （No. 81273143）.
文摘Background PB1-F2 protein has been proven to increase the pathogenicity of influenza A virus （IAV） strains in primary infection and in secondary bacterial infection. It can also regulate the activity of viral polymerase. However, it was shown in another retrospective study that a portion of IAVs do not express full-length PB1-F2 protein during virus development; different kinds of stop codons cause exits in the open reading frames and form PB1-F2 gene products with the corresponding genotypes. Truncated PB1-F2 in human H3N2 IAVs has long been detected in North America but its evolution in China is still unclear. Methods Influenza-like illnesses （ILls） from the whole of Jiangsu Province were collected and inspected to determine the type and subtype of the viruses. A portion of isolates collected in the epidemic period were selected as samples for later whole-genome sequencing, and the exact sequences were determined and analyzed. Results H3N2 influenza virus was one of the epidemical strains which had been prevalent during 2009-2010, in Jiangsu. Five H3N2 isolates with truncated PB1-F2 protein （25aa） were detected in influenza samples from Nanjing and Xuzhou, while seven similar H3N2 isolates were also reported in Niigata, Japan. Conclusion This emergence indicates the possibility that there has been transmission of the H3N2 virus between the two countries.
基金Supported by National Natural Science Foundation of China(Grant No.30872398)Applied Basic Research Program of Yunnan(Grant No.2007C032M,2010ZC055)Scientific Research Fund Project of Yunnan Provincial Department of Education(Grant No.2010Y398)
