THE STUDY OF ELEMENE OF INDUCTION APOPTOSIS ON ASCITES HEPATOMA CELL LINE Hca-F25/CL-16A3

Objective: To investigate the effect of inducing apoptosis of Elemene on ascites hepatoma cell line Hca F25/cL 16A3. By using immunhistochemistry and DNA electrophoresis, the mechanism of Elemene antitumor was studied. Results: The results showed that the Elemene can inhibit expression of bcl 2 in ascites hepatoma cell line Hca F25/CL 16A3, and the Elemene also can make DNA fragmentation in this cell line in vitro and in vivo . Conclusion: The data suggest that Elemene can inhibit the growth of tumor by inducing apoptosis.

两个不同转移力小鼠肝癌细胞系的细胞遗传学比较

Hca-F25/CL-16A_3(以下简称16A_3)和Hca-F25/CL-A_2(以下简称A_2)是两个来自共同祖先Hca-F25/L的具特异性淋巴道转移能力的小鼠腹水型肝癌细胞系。转移率前者为78.6～89.4％,后者为15～15.4％。通过细胞遗传学某些指标的观察,我们发现两系细胞存在着差异。核型分析发现,A_2系众数为42,标记染色体有四种,各一条;16A_3系众数为81和84,标记染色体也有四种,各双份,显带带型与A_2系之标记染色体完全相同。对银染核仁组织者区的观察发现:问期细胞的Ag-NORs数平均为:A_2 44个、16A_368个;中期细胞Ag-NORs平均数为:A_28.4个、16A_3 13.8个,高转移力的16_3A细胞明显高于低转移力的A_2细胞。结果提示:16A_3细胞可能是在祖先群体中由A_2细胞经核内复制而来;一些基因(如γDNA)的倍增和活性表达与16A_3高转移能力的形成可能有关。